Regulation of aging and age-related disease by DAF-16 and heat-shock factor   总被引：1，自引：0，他引：1  

Hsu AL  Murphy CT  Kenyon C 《Science (New York, N.Y.)》2003,300(5622):1142-1145

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Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocyst     

Hwang WS  Ryu YJ  Park JH  Park ES  Lee EG  Koo JM  Jeon HY  Lee BC  Kang SK  Kim SJ  Ahn C  Hwang JH  Park KY  Cibelli JB  Moon SY 《Science (New York, N.Y.)》2004,303(5664):1669-1674

Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells.  相似文献   

Nod2 mutation in Crohn's disease potentiates NF-kappaB activity and IL-1beta processing   总被引：1，自引：0，他引：1  

Maeda S  Hsu LC  Liu H  Bankston LA  Iimura M  Kagnoff MF  Eckmann L  Karin M 《Science (New York, N.Y.)》2005,307(5710):734-738

Variants of NOD2, an intracellular sensor of bacteria-derived muramyl dipeptide (MDP), increase susceptibility to Crohn's disease (CD). These variants are thought to be defective in activation of nuclear factor kappaB (NF-kappaB) and antibacterial defenses, but CD clinical specimens display elevated NF-kappaB activity. To illuminate the pathophysiological function of NOD2, we introduced such a variant to the mouse Nod2 locus. Mutant mice exhibited elevated NF-kappaB activation in response to MDP and more efficient processing and secretion of the cytokine interleukin-1beta (IL-1beta). These effects are linked to increased susceptibility to bacterial-induced intestinal inflammation and identify NOD2 as a positive regulator of NF-kappaB activation and IL-1beta secretion.  相似文献   

Forming electrical networks in three dimensions by self-assembly     

Gracias DH  Tien J  Breen TL  Hsu C  Whitesides GM 《Science (New York, N.Y.)》2000,289(5482):1170-1172

Self-assembly of millimeter-scale polyhedra, with surfaces patterned with solder dots, wires, and light-emitting diodes, generated electrically functional, three-dimensional networks. The patterns of dots and wires controlled the structure of the networks formed; both parallel and serial connections were generated.  相似文献   

Diversity and extinction of tropical american mollusks and emergence of the isthmus of panama     

Jackson JB  Jung P  Coates AG  Collins LS 《Science (New York, N.Y.)》1993,260(5114):1624-1626

The gradual closure of the Panamanian seaway and the resulting environmental change stimulated an increase in Caribbean molluscan diversity rather than the mass extinction hypothesized previously on the basis of inadequate data. Upheaval of molluscan faunas did occur suddenly throughout tropical America at the end of the Pliocene as a result of more subtle, unknown causes. There is no necessary correlation between the magnitude of regional shifts in abiotic conditions and the subsequent biological response.  相似文献   

Expression of interleukin-10 activity by Epstein-Barr virus protein BCRF1   总被引：34，自引：0，他引：34  

D H Hsu  R de Waal Malefyt  D F Fiorentino  M N Dang  P Vieira  J de Vries  H Spits  T R Mosmann  K W Moore 《Science (New York, N.Y.)》1990,250(4982):830-832

Cytokine synthesis inhibitory factor (CSIF; interleukin-10), a product of mouse TH2 T cell clones that inhibits synthesis of cytokines by mouse TH1 T cell clones, exhibits extensive sequence similarity to an uncharacterized open reading frame in the Epstein-Barr virus BCRF1. Recombinant BCRF1 protein mimics the activity of interleukin-10, suggesting that BCRF1 may have a role in the interaction of the virus with the host's immune system.  相似文献   

Response     

Hsu CY  Li CW 《Science (New York, N.Y.)》1995,269(5232):1890

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Magnetoreception in honeybees     

Hsu CY  Li CW 《Science (New York, N.Y.)》1994,265(5168):95-97

Magnetoreception by honeybees (Apis mellifera) is demonstrated by such activities as comb building and homing orientation, which are affected by the geomagnetic field. In other magnetoreceptive species, iron oxide crystals in the form of magnetite have been shown to be necessary for primary detection of magnetic fields. Here it is shown that trophocytes, which are apparently the only iron granule-containing cells in honeybees, contain super-paramagnetic magnetite. These cells are innervated by the nervous system, which suggests that trophocytes might be primarily responsible for magnetoreception. Electron microscopy also shows cytoskeletal attachments to the iron granule membrane.  相似文献   

A magnetic nanoprobe technology for detecting molecular interactions in live cells     

Won J  Kim M  Yi YW  Kim YH  Jung N  Kim TK 《Science (New York, N.Y.)》2005,309(5731):121-125

Technologies to assess the molecular targets of biomolecules in living cells are lacking. We have developed a technology called magnetism-based interaction capture (MAGIC) that identifies molecular targets on the basis of induced movement of superparamagnetic nanoparticles inside living cells. Efficient intracellular uptake of superparamagnetic nanoparticles (coated with a small molecule of interest) was mediated by a transducible fusogenic peptide. These nanoprobes captured the small molecule's labeled target protein and were translocated in a direction specified by the magnetic field. Use of MAGIC in genome-wide expression screening identified multiple protein targets of a drug. MAGIC was also used to monitor signal-dependent modification and multiple interactions of proteins.  相似文献   

Graphoepitaxy of self-assembled block copolymers on two-dimensional periodic patterned templates     

Bita I  Yang JK  Jung YS  Ross CA  Thomas EL  Berggren KK 《Science (New York, N.Y.)》2008,321(5891):939-943

Self-assembling materials are the building blocks of bottom-up nanofabrication processes, but they need to be templated to impose long-range order and eliminate defects. In this work, the self-assembly of a thin film of a spherical-morphology block copolymer is templated using an array of nanoscale topographical elements that act as surrogates for the minority domains of the block copolymer. The orientation and periodicity of the resulting array of spherical microdomains are governed by the commensurability between the block copolymer period and the template period and is accurately described by a free-energy model. This method, which forms high-spatial-frequency arrays using a lower-spatial-frequency template, will be useful in nanolithography applications such as the formation of high-density microelectronic structures.  相似文献