Detection of hemorrhagic septicemia virus of salmonid fishes by use of an enzyme-linked immunosorbent assay containing high sodium chloride concentration and two noncompetitive monoclonal antibodies against early viral nucleoproteins.     

F A Sanz  J M Coll 《American journal of veterinary research》1992,53(6):897-903

Inclusion of high-ionic strength buffers helped us to develop a sandwich ELISA to detect hemorrhagic septicemia virus (HSV) in cell culture and infected trout tissue extracts. For maximal sensitivity of 0.1 to 0.2 ng/well/100 microliters or about 10 to 50 TCID50/well/100 microliters, trout extracts were diluted 1:1 and assayed for the earliest synthesized nucleoprotein N. Simultaneous binding of the N protein from HSV in the sample to the wells coated with monoclonal antibody (2D5 against the N protein) and to the peroxidase-labeled monoclonal antibody (2C9 against the N protein) proceeded during a 2-hour incubation at 20 to 22 C (room temperature). The response was linear between 6 to 60 ng/well of purified virus. Monoclonal antibodies used were noncompetitive with each other and reacted with F1, F2, 23.75, and 5 Spanish isolates of HSV, but not with infectious hematopoietic necrosis or infectious pancreatic necrosis viruses. Tissue specimens with low content of HSV virus may now be assayed directly without use of cell culture, rapidly, and with high precision, during the acute phase of the disease in salmonid fishes.  相似文献   

Does grass sickness cross the placenta? A preliminary study.     

K E Whitwell 《The British veterinary journal》1992,148(1):81-83

  相似文献   

Effects of predator-induced visual and olfactory cues on 0+ perch (Perca fluviatilis L.) foraging behaviour     

V. N. Mikheev  J. Wanzenböck  A. F. Pasternak 《Ecology of Freshwater Fish》2006,15(2):111-117

Abstract –  Foraging juvenile fish with relatively high food demands are usually vulnerable to various aquatic and avian predators. To compromise between foraging and antipredator activity, they need exact and reliable information about current predation risk. Among direct predator-induced cues, visual and olfactory signals are considered to be most important. Food intake rates and prey-size selectivity of laboratory-reared, naive young-of-the-year (YOY) perch, Perca fluviatilis , were studied in experiments with Daphnia magna of two size classes: 2.8 and 1.3 mm as prey and northern pike, Esox lucius , as predator. Neither total intake rate nor prey-size selectivity was modified by predator kairomones alone (water from an aquarium with a pike was pumped into the test aquaria) under daylight conditions. Visual presentation of pike reduced total food intake by perch. This effect was significantly more pronounced (synergistic) when visual and olfactory cues were presented simultaneously to foraging perch. Moreover, the combination of cues caused a significant shift in prey-size selection, expressed as a reduced proportion of large prey in the diet. Our observations demonstrate that predator-induced olfactory cues alone are less important modifiers of the feeding behaviour of naive YOY perch than visual cues under daylight conditions. However, pike odour acts as a modulatory stimulus enhancing the effects of visual cues, which trigger an innate response in perch.  相似文献   

Verification by polymerase chain reaction of vertical transmission of Theileria sergenti in cows   总被引：2，自引：0，他引：2       下载免费PDF全文

Byeong K. Baek  Kim B. Soo  Jin H. Kim  Jin Hur  Bou O. Lee  J. M. Jung  Misao Onuma  Anthony O. Oluoch  Chang-Hyun Kim    Ibulaimu Kakoma 《Canadian journal of veterinary research》2003,67(4):278-282

To evaluate the transplacental transfer of Theileria sergenti infection in cattle, we used DNA probes to detect T. sergenti in 6 pregnant cows and their calves. All the animals were monitored by parasitologic, serologic, and polymerase chain reaction (PCR) assays for a predicted 875-base-pair (bp) DNA product and a 684-bp amplicon detected by nested PCR in the blood and spleens of aborted fetuses. An open reading frame (ORF) starting at nucleotide 170 and terminating at position 1021 was shown to code for a polypeptide of 283 amino acid residues. All 6 dams and 5 calves were positive for T. sergenti in all tests. One calf was positive only with nested PCR. We conclude that transplacental transmission of T. sergenti is a significant problem. The relevance of the data in the programmed introduction of new (especially pregnant) animals into established clean herds needs serious consideration with regard to control of theileriosis and other tickborne diseases.  相似文献   

The use of a Giardia vaccine as an immunotherapeutic agent in dogs.          下载免费PDF全文

M E Olson  C J Hannigan  P F Gaviller    L A Fulton 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(11):865-868

Dogs (n = 13), which had failed to be cured of giardiosis following chemotherapeutic measures, were treated with a Giardia vaccine (2-3 injections). Clinical signs resolved between 16 and 42 days postvaccination and cessation of fecal cyst shedding was between 21 and 70 days. Vaccination is a potential method of treating giardiosis in dogs.  相似文献   

Site index estimation in Scots pine (Pinus sylvestris L.) stands in the High Ebro Basin (northern Spain) using soil attributes     

Bravo  F.; Montero  G. 《Forestry》2001,74(4):395-406

  相似文献   

A Preliminary Trial of Detomidine/Opiate Combination in the Horse     

B.S. Paton  K.W. Clarke 《Veterinary anaesthesia and analgesia》1986,14(1):44-52

  相似文献   

Canine distal renal tubular acidosis and urolithiasis   总被引：1，自引：0，他引：1  

D J Polzin  C A Osborne  F W Bell 《Veterinary Clinics of North America: Small Animal Practice》1986,16(2):241-250

Distal RTA is characterized by decreased distal renal tubular hydrogen ion secretion, decreased ability to acidify urine, hypercalciuria, hyperphosphaturia, hypocitraturia, and metabolic acidosis. Because of the resulting alterations in urine composition and pH, patients with distal RTA are predisposed to urolithiasis and renal calcification. Diagnosis of distal RTA is important because it is a potentially reversible disorder that, left untreated, may cause nephrocalcinosis, recurrent urolith formation, moderate to severe metabolic acidosis, and renal failure.  相似文献   

Bücheranzeigen     

Köftler  Dr. Rohmeder 《European Journal of Forest Research》1938,60(10):322-325

  相似文献   

Qntgegnung auf die im Abgeordnetenhaufe zu Berlin in der Sisung vom 3. Decbr. 1867 gegen das hannover’fche Forftmefen gefallenen Aeuserungen. Qin Schreiben des Forftdirectors Burdhardt an bie Mitglieder bes Landtags aus der Brobing Hannober. 14 Seiten in 4°. Hannober. Q. Rümpler 1868. Breis 18 fr     

F. Baur 《European Journal of Forest Research》1868,12(1):113-120

Ohne Zusammenfassung  相似文献