BRUCELLA OVIS INFECTION   总被引：4，自引：0，他引：4  

K. G. Haughey  B.V.Sc.  K. L. Hughes  M.V.Sc.  Dip.Bact. W. J. Hartley  M.V.Sc.  M.R.C.V.S. 《Australian veterinary journal》1968,44(12):531-535

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The recovery after oral infection of immature Fasciola hepatica from the peritoneal cavity of two strains of mice     

E Harness  T G Doy  D L Hughes 《Research in veterinary science》1973,15(3):393-395

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GROWTH STIMULATION OF CABBAGE BY DIELDRIN   总被引：1，自引：1，他引：0  

W. A. Hughes 《Plant pathology》1960,9(4):149-149

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Toxicity of arsenic present in lakeweed   总被引：2，自引：0，他引：2  

R J Lancaster  M R Coup  J W Hughes 《New Zealand veterinary journal》1971,19(7):141-145

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Progesterone concentrations in the peripheral plasma of pregnant ewes following infection with an abortifacient organism     

I D Smith  K L Hughes 《Research in veterinary science》1974,16(1):116-118

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Immune electron microscopy of transmissible gastroenteritis virus and rotavirus (reovirus-like agent) of swine.   总被引：8，自引：0，他引：8  

L J Saif  E H Bohl  E M Kohler  J H Hughes 《American journal of veterinary research》1977,38(1):13-20

Immune electron microscopy (IEM) was developed as a diagnostic aid for detecting and identifying transmissible gastroenteritis virus and rotavirus (reovirus-like agent) in fecal and intestinal contents from cases of gastroenteritis in young pigs. Variables involved in use of direct IEM and its sensitivity were determined. Aggregates of virus coated with specific antibody were seen in virus samples mixed with homologous convalescent antiserum, but not in control samples containing preexposure serum or antibody directed against a heterologous virus. At least a ten fold enhancement of the sensitivity of direct IEM for virus detection was accomplished using indirect IEM employing rabbit anti-porcine IgG to further aggregate virus-antibody complexes. The technique was used to investigate the size and morphology of the porcine rotavirus. Particles ranged from 55 to 70 nm in diameter and had capsomere structures. Morphologically, the porcine rotavirus resembled the calf and human rotaviruses. By IEM, employing specific antiserums for each virus, porcine rotavirus was found to be antigenically related to these 2 viruses, but not to the reovirus type 3.  相似文献   

A Manual of the Care and Treatment of Children's and Exotic Pets, Edited by A. F. Cowie     

Ian Hughes 《The Journal of small animal practice》1976,17(10):711-712

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Ecology of Rhodococcus equi   总被引：7，自引：0，他引：7  

M D Barton  K L Hughes 《Veterinary microbiology》1984,9(1):65-76

A selective broth enrichment technique was used to study the distribution of Rhodococcus equi in soil and grazing animals. Rhodococcus equi was isolated from 54% of soils examined and from the gut contents, rectal faeces and dung of all grazing herbivorous species examined. Rhodococcus equi was not isolated from the faeces or dung of penned animals which did not have access to grazing. The isolation rate from dung was much higher than from other samples and this was found to be due to the ability of R. equi to multiply more readily in dung. Delayed hypersensitivity tests were carried out on horses, sheep and cattle, but only horses reacted significantly. The physiological characteristics of R. equi and the nature of its distribution in the environment suggested that R. equi is a soil organism.  相似文献   

Cloning and sequencing of badger (Meles meles) interferon gamma and its detection in badger lymphocytes     

Dalley DJ  Hogarth PJ  Hughes S  Hewinson RG  Chambers MA 《Veterinary immunology and immunopathology》2004,101(1-2):19-30

The European badger (Meles meles) has been identified as a reservoir for Mycobacterium bovis and is implicated in the maintenance and transmission of tuberculosis in cattle. There is a need for a sensitive test of M. bovis infection in badgers and the current serodiagnostic test used for this purpose has low sensitivity. As observed for other species, assay of interferon-gamma (IFNgamma) produced in response to M. bovis antigens is a more sensitive test of tuberculosis. With this objective in sight, we report the first step in the development of an ELISA for badger IFNgamma. The badger IFNgamma gene was cloned and sequenced and used to generate a specific polyclonal antibody to the cytokine. The gene sequence demonstrated regions that were conserved within the IFNgamma genes of other mammals. The badger sequence was most similar to the canine, showing similar structural organisation of the gene and 88% amino acid identity. Rabbits were immunised with DNA encoding badger IFNgamma and the resulting polyclonal antiserum demonstrated specificity for canine IFNgamma by immunoblot of a commercial recombinant canine IFNgamma. The antiserum was used to detect intracellular badger IFNgamma by flow cytometry analysis of badger lymphocytes stimulated with mitogen.  相似文献   

Ecology. Globalization, roving bandits, and marine resources     

Berkes F  Hughes TP  Steneck RS  Wilson JA  Bellwood DR  Crona B  Folke C  Gunderson LH  Leslie HM  Norberg J  Nyström M  Olsson P  Osterblom H  Scheffer M  Worm B 《Science (New York, N.Y.)》2006,311(5767):1557-1558

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