Screening and optimization of Andrographis paniculata (Burm.f.) Nees for total andrographolide content,yield and its components     

M.K. Bhan  A.K. DharS. Khan  S.K. LattooK.K. Gupta  D.K. Choudhary 《Scientia Horticulturae》2006

Andrographis paniculata (Burm.f.) Nees (Acanthaceae), commonly known as Kalmegh is used both in Ayurvedic and Unani system of medicines for a number of ailments related to digestion, hepatoprotection, hypoglycemic, and as anti-bacterial, analgesic, anti-inflammatory, vermicidal and antiacene. Ten accessions of A. paniculata from different sources were screened to assess the yield and its components besides andrographolides on three harvesting dates viz. 70, 100, 130 days after plantation corresponding to calendar months of September, October and November. The results revealed its successful growth in the subtropical region of Jammu, with last week of October ideal for obtaining maximum dry herbage (931.3 kg/ha) and total andrographolide yield (61.83 kg/ha), respectively. On the basis of leaf/stem ratio the relative estimates of total andrographolide is higher in October (46%) than September (15%) or November (39%). Accession Acc.1 and Acc.9 have been identified as the best sources for obtaining higher drug yield.  相似文献   

Genetics and molecular mapping of gynoecious (F) locus in cucumber (Cucumis sativus L.)     

Gograj Singh Jat  Anilabh Das Munshi  Tusar Kanti Behera  Harshwardhan Choudhary  Prasanta Dash  Amarnath Ravindran 《The Journal of Horticultural Science and Biotechnology》2019,94(1):24-32

Gynoecious is an important economic trait of cucumber for determinant of earliness and yield, yet genetic mechanism is not well understood for this trait. The experiment was conducted using F₂ mapping population by crossing of PPC-2, a gynoecious and parthenocarpic line with Pusa Uday (monoecious and non-parthenocarpic cultivar). Out of 179 SSR markers screened, 39 markers differentiated the gynoecious and monoecious parents. However, only 17 markers were segregating with F₂ mapping population, those were used for genotyping and linkage map analysis and these markers were placed along with F locus on chromosome 6 covering a total distance of 100.4cM. The SSR markers, SSR13251 and UW020605 were found to be closely linked to gynoecious (F) locus at 1.0 and 4.5 cM, respectively. The segregation of F₂ population of PPC-2 × Pusa Uday and GPC-1 × Punjab Naveen and test crosses for sex type herein suggested that single dominant gene controlled the gynoecious sex expression in cucumber particularly in gynoecious genotypes PPC-2 and GPC-1. Therefore, the monogenic dominant nature of gynoecious sex identified in the present experiment and SSR markers closely linked to the F locus will be useful in marker-assisted backcross breeding for transfering gynoecious trait into horticulturally desirable varieties.  相似文献   

Preliminary observations on biological activity of pure aflatoxin B1 in chick embryos     

P G Choudhary  S L Manjrekar 《The Indian veterinary journal》1967,44(7):543-548

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Effects of harvesting on productivity of bay leaf tree （Cinnamomum tamala Nees ＆ Eberm）： Case from Udayapur district of Nepal     

Dyutiman Choudhary  S.P Kala  N.P Todaria  S. Dasgupta  M. Kollmair 《林业研究》2014,25(1):163-170

Cultivation of bay leaves （Cinnamomum tamalaNees ＆ Eberm） to fulfil household income needs is a long established practice in Udayapur district of Nepal. The practices adopted by farmers for bay leaf harvesting have not, however, been validated by scientific investigation for their sustainability. To investigate the impacts of harvesting on the yield of branch, leaves and biomass of leaves, a two-year research project was conducted in farm fields at Kopche village of Routa VDC in Udayapur district, Nepal. Four different harvesting treatments, the orien-tation and the order of branches were taken as independent variables to test their effects on number of branches, leaves and biomass of leaves. Orientation, harvesting treatments and order of branches had a significant effect on the number of branches, but not on the number of leaves or biomass （fresh and dry weight） of leaves in the year of harvest. Between two consecutive harvests there was no significant difference in the num-ber of branches, leaves or biomass. Lower two-thirds portion of the trees produced the largest number of leaves and branches of the fourth order in both years. Therefore, lower two-thirds portion of the trees were suitable for harvesting. Our findings support farmer experience that no change in productivity of leaves is observed when harvesting each year. For long term sustainability, harvesting should be conducted without debarking of trees or damage to branches. Our findings could be extrapolated to and tested in other areas with different access and user rights where the rota-tion for harvest is fixed or regulated without research evidence.  相似文献   

Seropositivity of Toxoplasma gondii in domestic donkeys (Equus asinus) and isolation of T. gondii from farm cats     

J.P. Dubey  S.L. Ness  O.C.H. Kwok  S. Choudhary  L.D. Mittel  T.J. Divers 《Veterinary parasitology》2014,199(1-2):18-23

Donkeys (Equus asinus) are used as both companion and working animals throughout the world and in some countries, their meat and milk are used for human consumption. Here we report the first serological survey of Toxoplasma gondii in donkeys in the United States. Serum samples from 373 donkeys from eight farms in five states were tested for T. gondii antibodies by the modified agglutination test (MAT). Twenty-four of 373 (6.4%) of donkeys were seropositive, with MAT titers ranging from 25 to ≥200. All seropositive donkeys were Miniature breed. Seropositivity prevalence was 7.0% in female donkeys (20/282) and 4.1% in male donkeys (4/91). No donkeys less than 24 months of age (129) were seropositive, suggesting postnatal transmission of infection. Domestic cats were present on six of the eight farms. Three cats from one farm had MAT titers of 200. Viable T. gondii was isolated from the hearts of two cats, but not from brain tissues. Genotyping of isolate DNA extracted from culture-derived tachyzoites using 10 PCR-restriction fragment length polymorphism (RFLP) markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, PK1, L358 and Apico loci) revealed that both isolates were clonal Type II (ToxoDB PCR-RFLP genotype #1). This is the first serological survey for T. gondii in donkeys in the United States, and suggests that donkey milk and meat should be considered as a potential source for human infection. The role of barn cats in the transmission of T. gondii to donkeys on farms warrents further investigation.  相似文献   

Conservation tillage and seeding systems in the South Pacific     

M.A. Choudhary  C.J. Baker 《Soil & Tillage Research》1993,27(1-4):283-302

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Retrospective analysis of 736 cases of canine gastric dilatation volvulus     

KK Song  SE Goldsmid  J Lee  DJ Simpson 《Australian veterinary journal》2020,98(6):232-238

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Towards marker‐assisted selection in pulses: a review     

Jitendra Kumar  Arbind K. Choudhary  Ramesh K. Solanki  Aditya Pratap 《Plant Breeding》2011,130(3):297-313

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Herbicide resistant Phalaris minor in India—history of evolution,present status and its management     

Soni  Jeetendra Kumar  Nibhoria  Amarjeet  Punia  S. S.  Yadav  Dharam Bir  Choudhary  V. K.  Lalramhlimi  B.  Navik  Omprakash 《Phytoparasitica》2023,51(2):353-378

Phytoparasitica - The introduction and large scale cultivation of wheat cultivars which are semi-dwarf and high yielding in the north-western Indo-Gangetic Plains (IGPs) of India has led to the...  相似文献   

Association mapping,trait variation,interaction and population structure analysis in cucumber (Cucumis sativus L.)     

Kumar  Rahul  Das Munshi  Anilabh  Behera  Tusar Kanti  Jat  Gograj Singh  Choudhary  Harshawardhan  Talukdar  Akshay  Dash  Prasanta  Singh  Deepak 《Genetic Resources and Crop Evolution》2022,69(5):1901-1917

Genetic Resources and Crop Evolution - In several regions of the world, low productivity in this crop is attributed to several factors including poor understanding of the genomic complexity of...  相似文献