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51.
Vaccination of four calves with Leptavoid (Wellcome New Zealand Limited) gave rise to Leptospira interrogans serovars hardjo and pomona microscopic agglutination test titres that could not be distinguished in magnitude from post-infection titres. Vaccination of four calves with Lepto-3 (ICI Tasman Limited) gave rise to much lower titres.

Revaccination of cows with Leptavoid caused a rise in hardjo titres which was significantly greater than after use of Lepto-3.

The possibility that titres were due to the simultaneous infection with serovars pomona and hardjo of only the animals vaccinated with Leptavoid must he discounted.  相似文献   
52.
Worldwide, most sea cucumber fisheries are ineffectively managed, leading to declining stocks and potentially eroding the resilience of fisheries. We analyse trends in catches, fishery status, fishing participation and regulatory measures among 77 sea cucumber fisheries through data from recent fishery reports and fishery managers. Critical gaps in fisheries biology knowledge of even commonly targeted species undermine the expected success of management strategies. Most tropical fisheries are small‐scale, older and typified by numerous (>8) species, whereas temperate fisheries are often emerging, mono‐specific and industrialized. Fisher participation data indicated about 3 million sea cucumber fishers worldwide. Fisher participation rates were significantly related to the average annual yield. permanova analysis showed that over‐exploited and depleted fisheries employed different sets of measures than fisheries with healthier stocks, and a non‐metric multidimensional scaling ordination illustrated that a broad set of regulatory measures typified sustainable fisheries. SIMPER and regression tree analyses identified that the dissimilarity was most related to enforcement capacity, number of species harvested, fleet (vessel) controls, limited entry controls and rotational closures. The national Human Development Index was significantly lower in countries with over‐exploited and depleted fisheries. Where possible, managers should limit the number of fishers and vessel size and establish short lists of permissible commercial species in multispecies fisheries. Our findings emphasize an imperative to support the enforcement capacity in low‐income countries, in which risk of biodiversity loss is exceptionally high. Solutions for greater resilience of sea cucumber stocks must be embedded within those for poverty reduction and alternative livelihood options.  相似文献   
53.
The inclusion of legumes in semi‐arid native grasslands may promote the productivity and nutritive value of forage. This study was designed to assess the effect of legumes (the introduced legume Medicago sativa or the native legume Dalea purpurea) and soil P fertility (addition of 0, 50, or 200 P2O5 kg/ha at seeding) on the dry matter and nutrient content of native grasses mixtures, compared with the commonly used introduced forage grass Bromus biebersteinii grown with M. sativa. Plant harvests were performed in September 2008, July 2009 and September 2009. Plants nutrient content, δ15N value and dry matter were analysed. Results show that the M. sativa enhanced the N and P concentrations of native grass mixtures early in the summer, as well as the N concentration in Bouteloua gracilis in late summer of the driest year, 2009. The higher AM fungal diversity promoted by M. sativa was positively correlated with the dry matter and nitrogen uptake of M. sativa and with the P concentration of native grasses, in early summer. Overall, this study shows that M. sativa promoted beneficial AM fungal taxa and improved forage production in the semi‐arid prairies.  相似文献   
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The suspension‐feeding sea cucumber Cucumaria frondosa is widely distributed in cold waters and is commercially exploited in the North Atlantic. While the species is considered to have potential for aquaculture, its feeding and reproductive biology differs markedly from that of currently cultivated sea cucumbers. Here, for the first time, the influence of food sources on the condition of C. frondosa was experimentally tested. Individuals were fed with either diatoms or fish eggs for 3 months. Specific growth rate (SGR), organ indices, fecundity, gonad maturity and profiles of lipids and fatty acids (FA) in tissues were compared among treatments and with sea cucumbers collected from the field. Individuals fed with fish eggs showed higher SGR and organ indices than all other treatments. The highest proportion of large oocytes was also found in gonad tubules of females from the fish egg treatment, although individuals fed with diatoms were the only ones in which spontaneous spawning occurred. Moreover, gonad and muscle tissues of sea cucumbers from the fish egg treatment presented the highest levels of lipids and essential FA. In contrast, non‐fed sea cucumbers showed negative SGR, relatively low female fecundity and low concentrations of lipids and FA in tissues. While the fish egg diet presented several obvious benefits, phytoplankton remains an important source of carotenoids, which are vital for vitellogenesis in echinoderms. This indicates that mixed diets rich in lipids, essential FA and carotenoids can be further investigated to optimize growth and reproductive output of this species in captivity.  相似文献   
56.
No-tillage systems contribute to physical, chemical and biological changes in the soil. The effects of different tillage practices and phosphorus (P) fertilization on soil microbial biomass, activity, and community structure were studied during the maize growing season in a maize–soybean rotation established for 18 years in eastern Canada. Soil samples were collected at two depths (0–10 and 10–20 cm) under mouldboard plow (MP) and no-till (NT) management and fertilized with 0, 17.5, and 35 kg P ha?1. Results show that the duration of the growing season had a greater effect on soil microbiota properties than soil tillage or P fertilization at both soil depths. Seasonal fluctuations in soil microbial biomass carbon (SMB-C) and nitrogen (SMB-N), in dehydrogenase and alkaline phosphomonoesterase activities, and in total phospholipids fatty acid (PLFA) level, were greater under NT than MP management. The PLFA biomarkers separated treatments primarily by sampling date and secondly by tillage management, but were not significantly affected by P fertilization. The abundance of arbuscular mycorrhizal fungi (AMF; C16:1ω5) and fungi (C18:2ω6,9) was lower under NT than MP at the 10–20-cm soil depth in July. Phosphorus fertilization increased soil microbial biomass phosphorus (SMB-P) and Mehlich-3 extractable P, but had a limited impact on the other soil properties. In conclusion, soil environmental factors and tillage had a greater effect on microorganisms (biomass and activity) and community structure than P fertilization.  相似文献   
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SUMMARY The effects of induced parturition on calf mortality rates were studied in 24 winter-calving, pasture-fed, dairy herds in south-western Victoria. Parturition was induced when most cows were between 27 and 35 weeks of pregnancy. Control groups were selected from cows in the same herds in which calving was not induced. The incidence of mortality within 7 days of birth was much higher among calves from cows in induced groups relative to those in control groups (estimated median herd mortality incidence 72% and 7%, respectively). The incidence of calf mortality was high among groups induced at all stages of pregnancy that were investigated. However, differences in calf mortality incidence, relative to controls, were increased among groups induced at earlier stages of pregnancy.  相似文献   
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SUMMARY A combination of tiletamine hydrochloride and zolazepam hydrochloride in a 1:1 ratio by weight was used successfully to sedate mountain brushtail possums, Trichosurus caninus, in the field. A standard total dose of 50 to 60 mg provided adequate sedation for the completion of a range of handling procedures. We describe the induction time, dose rate and side-effects associated with the use of tiletamine and zolazepam in T caninus.  相似文献   
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