Multi-drug resistant Escherichia coli with extended-spectrum β -lactamase activity and fluoroquinolone resistance isolated from clinical infections in dogs     

AL WARREN  KM TOWNSEND  T KING  S MOSS  D O''BOYLE  RM YATES  DJ TROTT 《Australian veterinary journal》2001,79(9):621-623

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Understanding Brown Planthopper Resistance in Rice: Genetics,Biochemical and Molecular Breeding Approaches     

Muduli Lakesh  Kumar Pradhan Sukanta  Mishra Abinash  Nath Bastia Debendra  Chandra Samal Kailash  Kumar Agrawal Pawan  Dash Manasi 《水稻科学》2021,28(6):532-546

Brown planthopper (BPH, Nilaparvata lugens Stål) is the most devastating pest of rice in Asia and causes significant yield loss annually. Around 37 BPH resistance genes have been identified so far in indica, African rice varieties along with wild germplasms such as Oryza officinalis, O. minuta, O. nivara, O. punctata, O. rufipogon and O. latifolia. Genes/QTLs involved in BPH resistance, including Bph1, bph2/BPH26, Bph3, Bph6, bph7, BPH9, Bph12, Bph14, Bph15, Bph17, BPH18, bph19, Bph20, Bph21(t), Bph27, Bph27(t), Bph28(t), BPH29, QBph3, QBph4, QBph4.2, Bph30, Bph32, Bph33, Bph35 and Bph36, have been fine-mapped by different researchers across the globe. The majority of genes/QTLs are located on rice chromosomes 1, 3, 4, 6, 11 and 12. Rice plants respond to BPH attack by releasing various endogenous metabolites like proteinase inhibitors, callose, secondary metabolites (terpenes, alkaloids, flavonoid, etc.) and volatile compounds. Besides that, hormonal signal pathways mediating (antagonistic/synergistic) resistance responses in rice have been well studied. Marker-assisted breeding and genome editing techniques can also be adopted for improving resistance to novel BPH biotypes.  相似文献   

Effect of spraying selected pesticides on the contents of specified minerals in cabbage     

Snehalatha Reddy  N.  Dash  Sagarika  Sontakke 《Plant foods for human nutrition (Dordrecht, Netherlands)》1997,51(4):357-363

The present study was conducted to assess the effect of spraying of pesticides on the content of minerals in cabbage harvested at varying waiting periods (0, 7, 14 and 21 days) after spraying. The amounts of phosphorus and zinc in cabbage were significantly (p < 0.05) decreased while those of iron, calcium and potassium were significantly (p < 0.05) increased by the sprayings of pesticides. At varying waiting periods after the spraying of pesticides calcium, phosphorus, zinc and potassium contents of cabbage were found to be decreased remarkably (p < 0.05) while the content of iron was increased significantly (p < 0.05). Between the two pesticides, the spraying of endosulfan exerted a more pronounced effect in altering the contents of minerals than the spraying of malathion.  相似文献   

Mycotic dermatitis with digital gangrene and osteomyelitis,and protozoal intestinal parasitism in Marlborough green geckos (Naultinus manukanus)     

BD Gartrell  KM Hare 《New Zealand veterinary journal》2013,61(5):363-367

CASE HISTORY: Thirty adult Marlborough green geckos (Naultinus manukanus) were collected from Stephens Island and held over winter, prior to their translocation. Five adult geckos developed skin lesions after husbandry changes affected the humidity of their enclosures. Two geckos underwent ecdysis and recovered. One animal died and two others progressively worsened and were presented for treatment.

CLINICAL AND PATHOLOGICAL FINDINGS: The geckos were in poor body condition and had multiple black powdery lesions and solitary raised white nodules on their skin. Both geckos died despite topical and supportive treatment. Histopathology showed the skin nodules contained branching non-septate hyphae infiltrating necrotic epidermal tissue, and associated dermal inflammation. There was necrosis of several digits and mycotic osteomyelitis. Mucor ramosissimus was cultured from skin biopsies from each animal. Large numbers of motile protozoa, resembling Trichomonas, and another unidentifiable, were recovered from fresh faecal smears, and Nyctotherus sp protozoa were present in the lumen of the intestine of one animal post mortem.

DIAGNOSIS: Mycotic dermatitis with digital gangrene and osteomyelitis due to Mucor ramosissimus, and enteric protozoal parasitism with Trichomonas sp and Nyctotherus sp.

CLINICAL RELEVANCE: The clinical course and pathological findings of mycotic dermatitis in two Marlborough green geckos involved in a wildlife translocation in New Zealand are reported, and also the first record of the Marlborough green gecko as a host for the enteric protozoa Trichomonas sp and Nyctotherus sp.  相似文献   

Detection of Mycoplasma conjunctivae in sheep affected with conjunctivitis and infectious keratoconjunctivitis     

MXJ Motha  J Frey  MF Hansen  R Jamaludin  KM Tham 《New Zealand veterinary journal》2013,61(4):186-190

AIM: To determine the aetiolog y of a recurring and severe form of infectious keratoconjunctivitis (IKC) in sheep.

METHODS: Five sheep flocks that had experienced a severe form of IKC were examined. Clinical history, conjunctival swabs and blood samples were collected from affected animals. Culture for bacteria, and also specifically for Mycoplasma and Chlamydophila spp, and detection of Mycoplasma conjunctivae DNA by polymerase chain reaction (PCR) were attempted. Serum samples were tested for antibodies to M. agalactiae, M. capricolum, M. conjunctivae and Chlamydophila spp.

RESULTS: Mycoplasma conjunctivae DNA was detected using PCR in 3/5 flocks, and in all flocks antibodies to M. conjunctivae were detected in sera. A pure growth of Branhamella ovis was cultured from conjunctival swabs from a small proportion of sheep in two flocks. No other pathogens were detected.

CONCLUSIONS: This investigation demonstrated that M. conjunctivae was a primary pathogen causing severe IKC in sheep, and is the first report of detection of this organism in sheep in New Zealand. Introduction of clinically normal carrier sheep appeared to have caused the outbreaks.  相似文献   

Sensitivities to DMI fungicides in populations of Podosphaera fusca in south central Spain     

Francisco J López‐Ruiz  Alejandro Pérez‐García  Dolores Fernández‐Ortuño  Diego Romero  Emilio García  Antonio de Vicente  James KM Brown  Juan A Torés 《Pest management science》2010,66(7):801-808

BACKGROUND: Cucurbit powdery mildew elicited by Podosphaera fusca (Fr.) U Braun & N Shishkoff limits crop production in Spain. Disease control is largely dependent on fungicides such as sterol demethylation inhibitors (DMIs). Fungicide resistance is an increasing problem in this pathogen. To overcome such risk, it is necessary to design rational control programmes based upon knowledge of field resistance. The aim of this study was to investigate the state of DMI sensitivity of Spanish P. fusca populations and provide tools for improved disease management. RESULTS: Using a leaf‐disc assay, sensitivity to fenarimol, myclobutanil and triadimenol of 50 isolates of P. fusca was analysed to determine discriminatory concentrations between sensitive and resistant isolates. As no clearly different groups of isolates could be identified, discriminatory concentrations were established on the basis of maximum fungicide field application rate, 100 mg L^?1 for the three fungicides tested. Subsequently, a survey of DMI resistance was carried out in different provinces located in the south central area of Spain during the cucurbit growing seasons in 2002, 2003 and 2004. Examination of a collection of 250 isolates revealed that 23% were resistant to fenarimol and 7% to triadimenol, the provinces of Almería, Badajoz and Murcia being the locations with the highest frequencies of resistance. By contrast, no resistance to myclobutanil was found. CONCLUSION: Results show that fenarimol and, to a lesser extent, triadimenol have become less efficient for controlling cucurbit powdery mildew in Spain. These are important observations that should lead to reconsideration of the current disease management programmes. Copyright © 2010 Society of Chemical Industry  相似文献   

Retrospective study of 108 foals with septic osteomyelitis     

KM Neil  JE Axon  AP Begg  PG Todhunter  PL Adams  AE Fine  JP Caron  AR Adkins 《Australian veterinary journal》2010,88(1-2):4-12

Objective   To determine the clinical characteristics, short-term outcome and future athletic performance of foals with septic osteomyelitis.
Design   Retrospective clinical study of 108 Thoroughbred foals with radiographic evidence of bone infection that were presented at the Scone Veterinary Hospital between August 1995 and December 2001. Medical records were reviewed and information concerning signalment, the clinical, laboratory and radiographic findings, treatment and outcome was obtained. Racing records were obtained and evaluated for surviving foals that had reached racing age.
Results   Mean age of foals at initial evaluation was 39 days (range 1–180 days); 21 foals had multiple radiographic bone lesions (19.4%), and 76 had concurrent septic arthritis (70.4%). The most frequently affected bones were the femur, tibia and distal phalanx. In total, 87 foals were discharged from the hospital (80.6%), 79 survived long-term to reach racing age and 52 raced (65.8%). Overall, 48% (52/108) of the foals treated for osteomyelitis raced. Foals less than 30 days of age at the time of diagnosis, critically ill foals and those with multiple bones or joints affected were significantly less likely to be discharged from hospital. Multiple septic joints, but not multiple bone involvement, had an unfavourable prognosis for racing.
Conclusions   The prognosis for survival of foals with septic osteomyelitis or osteitis is favourable. Multiple bone or joint involvement is an important short-term prognostic indicator; however, the involvement of multiple joints, but not multiple infected bones, is associated with an unfavourable prognosis for racing.  相似文献   

Characterising the spatial pattern of phenology for the tropical vegetation of India using multi-temporal MERIS chlorophyll data     

C. Jeganathan  J. Dash  Peter M. Atkinson 《Landscape Ecology》2010,25(7):1125-1141

The annual growth cycles of terrestrial ecosystems are related to long-term regional/global climatic patterns. Understanding vegetation phenology and its spatio-temporal variation is required to reveal and predict ongoing changes in Earth system dynamics. The study attempts to characterize the phenology of the major tropical vegetation types in India, since such information is not yet available for India. Multi-temporal Medium Resolution Imaging Spectrometer (MERIS) Terrestrial Chlorophyll Index (MTCI) data were utilized to derive onset of greenness (OG) and end of senescence (ES) for four major tropical vegetation types. The study found that Fourier-smoothed results using the first four components revealed adequately the annual phenological variation of the natural vegetation types in India. From these smoothed data, inflection points were located iteratively through a spatio-temporal search, spanning over 18 months of 8-day composite data, per pixel such as to derive the OG and ES. The median OG and ES was extracted from the available annual results for the years 2003–04, 2004–05, 2005–06 and 2006–07. The GLC2000 land cover map (1 km spatial resolution) was utilized to determine the locations of the major vegetation types. The percentage of each vegetation type falling beneath a MTCI composite pixel (4.6 km spatial resolution) was calculated. MTCI composite pixels with homogeneity of ≥80% vegetative cover were used for examining pattern of phenology in different regions, different years and at different latitudes. The most common dates for the occurrence of OG for the tropical evergreen, semi-evergreen, moist-deciduous, and dry-deciduous vegetation types were found to be during February–April, January–April, March–May, and February–May, respectively. Similarly, for ES the most common dates were in February–April, January–April, February–April, and December–April, respectively. The phenological pattern was uniquely different for each vegetation type, as expected, and also differed with regions and latitudes. A general trend of early occurrence of OG in the lower latitudes was observed.  相似文献   

Epizootics of sudden death in tammar wallabies (Macropus eugenii) associated with an orbivirus infection     

KA Rose  PD Kirkland  RJ Davis  D Blumstein  LI Pritchard  KM Newberry  RA Lunt 《Australian veterinary journal》2012,90(12)

Epizootics of sudden death in tammar wallabies (Macropus eugenii) occurred at six research facilities and zoological gardens in New South Wales, Australia, in late 1998 and at one Queensland research facility in March 1999. There were 120 confirmed tammar wallaby deaths during this period; however, population censuses indicated that up to 230 tammar wallabies may have died. The majority of animals died without premonitory signs. A small proportion of wallabies exhibited increased respiratory rate, sat with a lowered head shortly before death or were discovered in lateral recumbency, moribund and with muscle fasciculations. Gross postmortem findings consistently included massive pulmonary congestion, mottled hepatic parenchyma and subcutaneous oedema throughout the hindlimbs and inguinal region. Approximately 30% of the animals examined also had extensive haemorrhage within the fascial planes and skeletal muscle of the hindlimb adductors, inguinal region, ventral thorax, dorsal cervical region and perirenal retroperitoneal area. The tissues of affected animals became autolytic within a short period after death. Bacteriological examination of tissues from 14 animals did not provide any significant findings. Toxicological examination of the gastric and colonic contents of four animals did not reveal evidence of brodifacoume or other rodenticides. Viruses from the Eubenangee serogroup of the Orbivirus genus were isolated from the cerebral cortex of nine, and the myocardium of two, tammar wallabies and the liver and intestine of another tammar wallaby. A similar orbivirus was also isolated from the cerebrospinal fluid of another tammar wallaby that died suddenly. The disease agent appears to be a previously unrecognised orbivirus in the Eubenangee serogroup. This is the first report of epizootics of sudden deaths in tammar wallabies apparently associated with an orbivirus infection.  相似文献   

Improving oil quality by altering levels of fatty acids through marker-assisted selection of <Emphasis Type="Italic">ahfad2</Emphasis> alleles in peanut (<Emphasis Type="Italic">Arachis hypogaea</Emphasis> L.)     

Sandip K. Bera  Jignesh H. Kamdar  Swati V. Kasundra  Pitabas Dash  Anil K. Maurya  Mital D. Jasani  Ajay B. Chandrashekar  N. Manivannan  R. P. Vasanthi  K. L. Dobariya  Manish K. Pandey  Pasupuleti Janila  T. Radhakrishnan  Rajeev K. Varshney 《Euphytica》2018,214(9):162

Peanut plays a key role to the livelihood of millions in the world especially in Arid and Semi-Arid regions. Peanut with high oleic acid content aids to increase shelf-life of peanut oil as well as food products and extends major health benefits to the consumers. In peanut, ahFAD2 gene controls quantity of two major fatty acids viz, oleic and linoleic acids. These two fatty acids together with palmitic acid constitute 90% fat composition in peanut and regulate the quality of peanut oil. Here, two ahfad2 alleles from SunOleic 95R were introgressed into ICGV 05141 using marker-assisted selection. Marker-assisted breeding effectively increased oleic acid and oleic to linoleic acid ratio in recombinant lines up to 44% and 30%, respectively as compared to ICGV 05141. In addition to improved oil quality, the recombinant lines also had superiority in pod yield together with desired pod/seed attributes. Realizing the health benefits and ever increasing demand in domestic and international market, the high oleic peanut recombinant lines will certainly boost the economical benefits to the Indian farmers in addition to ensuring availability of high oleic peanuts to the traders and industry.  相似文献