Predicting beef tenderness using near-infrared spectroscopy     

Rust SR  Price DM  Subbiah J  Kranzler G  Hilton GG  Vanoverbeke DL  Morgan JB 《Journal of animal science》2008,86(1):211-219

The objective of this multiple-phase study was to determine the accuracy of an on-line near-infrared (NIR) spectral reflectance system to predict 14-d-aged cooked beef tenderness. In phase I, 292 carcasses (140 US Select, 152 US Choice) were selected (d 2) from 2 commercial beef processing facilities. After carcass selection, longissimus lumborum (LL) muscle sections (ribs 9 to 12) were individually identified, vacuum-packaged, and transported to the Oklahoma State University Meats Laboratory, where a 2.54-cm-thick steak (n = 1) was fabricated and stored in refrigerated conditions (1 degrees C +/- 1). Following a 30-min oxygenation period, a NIR spectral scan was obtained on the 12th-rib LL steak. Steaks (d 3) were individually vacuum-packaged and aged at 4 degrees C for a total of 14 d before cooking slice shear force (SSF) analysis. In phases II and III, 476 carcasses (258 US Select, 218 US Choice) were immediately NIR scanned after carcass presentation to in-plant USDA grading personnel. In a similar fashion, all LL steaks were aged (1 degrees C +/- 1) for 14 d before cooking (70 degrees C) and conducting SSF. Of the phase I and II samples, 39 (6.77%) were categorized as being tough (i.e., >/= 25 kg of SSF after the 14-d postmortem aging period). Of these 39 tough samples, 20 (3.7% error rate) were correctly placed in the 90% certification level. Another 10 tough samples were placed in the 80% certification level (2.0% error rate). The overall NIR certified tender group was 1.67 kg more tender (P < 0.05) than LL samples from the noncertified samples. When the NIR predicted samples to be tough, 10% of the samples were eliminated from the phase I and II LL populations at 90% certification. The population SSF mean improved in excess of 6.5 kg. For phase III, SSF evaluation by an independent third party indicated the NIR system was able to successfully sort tough from tender LL samples to 70% certification levels. It was concluded that NIR scanning offers an in-plant opportunity to sort carcasses into tenderness outcome groups for guaranteed-tender branded beef programs.  相似文献   

Ostertagiasis in calves. I. The effect of control measures on infection levels and body weight gains during the grazing season in Denmark     

SV AA Henriksen  R.J. Jørgensen  P. Nansen  KR Sejrsen  J. Brolund Larsen  S. Klausen 《Veterinary parasitology》1976,2(3):259-272

The investigation was designed to study the gastro-intestinal helminth infection established in yearling calves with reference to fluctuations in the larval contamination of the herbage during the grazing season. In a 2 × 3 factor experiment, comprising six groups, each of six calves, a comparison was made between infection levels, growth rates etc. in calves grazing the same paddock over an entire season and calves moved before the July rise in herbage contamination to paddocks not grazed earlier in the same season. A comparison was also made between animals receiving no anthelminthic treatment, animals treated 3 weeks after the start of the grazing season and again when moved, and animals treated every 3 weeks during the season.Before July there were no weight gain differences between the various groups of calves. During the rest of the season, the calves which were moved gained 647–869 g, while those that remained on the same paddock, and were exposed to a high level of larval contamination gained only 81–361 g per day. The effect of anthelmintic treatment was less pronounced, though significant. The weight gains were correlated to a high degree with the larval contamination levels of the respective paddocks (r = ? 0.79) as well as with the serum pepsinogen (r = ? 0.75) and albumin levels (r = 0.75) of the calves and to a minor degree also with faecal egg counts (r = ? 0.45). The observed weight gain differences could not be accounted for by differences in grass quantity or quality.  相似文献   

The effects of DDT upon flat and vesicular bilayers studied by a fluorescent probe     

R.D. O&#x;Brien  T.J. Herbert  B.D. Hilton 《Pesticide biochemistry and physiology》1977,7(5):416-425

The effect of DDT and several analogs upon bilayer membranes was studied, using the fluorescent dye di (octadecyl)oxycarbocyanin as a probe. It was shown in flat bilayers of Torpedo electroplax phospholipids, using fluorescence correlation spectroscopy, that neither DDT nor its physiologically inactive analog, p,p′-hydroxy-DDT, affected the fluidity of the membrane. However DDT, and not the analog, displaced half of the dye from the membrane. In studies with lecithin or Torpedo electroplax phospholipid vesicles, using simple fluorescence measurements, no displacement of the dye was observed by DDT or its analogs.  相似文献   

Integrase inhibitors and cellular immunity suppress retroviral replication in rhesus macaques     

Hazuda DJ  Young SD  Guare JP  Anthony NJ  Gomez RP  Wai JS  Vacca JP  Handt L  Motzel SL  Klein HJ  Dornadula G  Danovich RM  Witmer MV  Wilson KA  Tussey L  Schleif WA  Gabryelski LS  Jin L  Miller MD  Casimiro DR  Emini EA  Shiver JW 《Science (New York, N.Y.)》2004,305(5683):528-532

We describe the efficacy of L-870812, an inhibitor of HIV-1 and SIV integrase, in rhesus macaques infected with the simian-human immunodeficiency virus (SHIV) 89.6P. When initiated before CD4 cell depletion, L-870812 therapy mediated a sustained suppression of viremia, preserving CD4 levels and permitting the induction of virus-specific cellular immunity. L-870812 was also active in chronic infection; however, the magnitude and durability of the effect varied in conjunction with the pretreatment immune response and viral load. These studies demonstrate integrase inhibitor activity in vivo and suggest that cellular immunity facilitates chemotherapeutic efficacy in retroviral infections.  相似文献   

Geochemistry. The leaking mantle     

Hilton DR 《Science (New York, N.Y.)》2007,318(5855):1389-1390

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Antagonism by DDT of the effect of valinomycin on a synthetic membrane     

B D Hilton  R D O'Brien 《Science (New York, N.Y.)》1970,168(933):841-843

The potassium conductance which is induced by 10(-6) molar valinomycin in a lecithin-decane membrane is reversed by 3 x 10(-6) molar DDT. Membranes not treated with valinomycin are not affected by DDT. This blockade of potassium conductance parallels the effect of DDT on axonic conduction. Dieldrin and lindane, whose physiological actions are in some ways like those of DDT, do not affect valinomycin-induced conductance of lecithin-decane membranes.  相似文献   

Evaluation of pheromone release from commercial mating disruption dispensers     

Tomaszewska E  Hebert VR  Brunner JF  Jones VP  Doerr M  Hilton R 《Journal of agricultural and food chemistry》2005,53(7):2399-2405

Pome fruit growers and crop consultants have expressed concerns about the seasonal release performance of commercial codling moth mating disruption dispenser products. Because of these concerns, we developed a laboratory flow-through volatile collection system (VCS) for measuring the volatile release of the codling moth sex pheromone, codlemone, from commercially available hand-applied dispensers. Under controlled air-flow and temperature conditions, the released vapor was trapped onto a polyurethane foam adsorbent followed by solvent extraction, solvent reduction, and GC/MS determination. Method recovery and breakthrough validations were performed to demonstrate system reliability before determining codlemone release from commercial dispensers field-aged over 140 days. The volatile collection was carried out in a consistent manner among five dispenser types most commonly used by growers, so that direct comparison of performance could be made. The comparison showed differences in the amount of pheromone released and in the patterns of release throughout the season between dispenser types. The variation in release performance demonstrates the need for routine evaluation of commercially marketed mating disruption dispensers. We believe that the simple and cost-effective volatile collection system can assist pheromone dispenser manufacturers in determining seasonal dispenser performance before new products are introduced into the commercial market and in rapidly verifying dispenser release when field-aged dispenser efficacy is in question.  相似文献   

Nitrate leaching from short-rotation coppice   总被引：1，自引：0，他引：1  

G. Goodlass    M. Green    B. Hilton  & S. McDonough   《Soil Use and Management》2007,23(2):178-184

In the UK, short‐rotation coppice (SRC) is expected to become a significant source of ‘bio‐energy’ over the next few years. Thus, it is important to establish how nitrate leaching losses compare with conventional arable cropping, especially if SRC is grown in Nitrate Vulnerable Zones. Nitrate leaching was measured using porous ceramic cups in each of the three phases in the lifespan of SRC, establishment, harvest and removal and was compared with conventional arable cropping. Nitrogen concentrations were increased in drainage water as soon as the crop cover was destroyed to plant the SRC (peak 70 mg L^?1 nitrate‐N) and increased further (peak 134 mg L^?1 nitrate‐N) on cultivation. Once the coppice crop was established, concentrations returned to a smaller level (average 18 mg L^?1 nitrate‐N). Concentrations were not affected by the harvesting operation, and annual applications of nitrogen (40, 60 and 100 kg ha^?1 N in the first, second and third years, respectively) had little effect. By contrast, concentrations in the arable rotation showed a regular pattern of increase in the autumn, and the average peak value over the 4 years was 54 mg L^?1 nitrate‐N. When the SRC was ‘grubbed up’ and roots removed, the soil disturbance resulted in a flush of mineralization which, combined with a lack of crop cover, led to increased nitrate‐N in leachate (peak 67 mg L^?1 nitrate‐N). In a normal life‐span of SRC (15–30 years), the relatively large nitrate losses on establishment and at final grubbing up would be offset by small losses during the productive harvest phase, especially when compared with results under the arable rotation.  相似文献   

The conservation status of the spectacled petrel Procellaria conspicillata     

Peter G. Ryan  Clifford Dorse  Geoff M. Hilton 《Biological conservation》2006,131(4):575-583

The spectacled petrel Procellaria conspicillata is listed as critically endangered due to its small population size and ongoing mortality on long-lines. Spectacled petrels were counted in 2004, repeating a census made in 1999 at their sole breeding locality, Inaccessible Island. The 2004 survey took place earlier in the breeding season than the previous count, allowing for more robust estimates of burrow occupancy. During early incubation, birds responded to call playback at 69% of burrow entrances, but birds in at least 8% of burrows remained silent. Birds in shallow burrows were less likely to respond to playback than were those in deep burrows. Two repeat trials at 100 marked nests showed that at least 61% of apparently ‘unoccupied’ burrows were occupied on subsequent checks, resulting in an overall occupancy estimate of 91%. Occupancy was equally high in peripheral colonies. The apparent spatial extent of colonies increased slightly from 1999, and the estimate of total burrow numbers increased by 50%, from 5900 burrows in 1999 to 8900 in 2004. Validation surveys indicated that burrow numbers were underestimated (84 ± 3%) to the same extent as that in 1999 (85 ± 4%), and repeat checks of one colony where all nests were marked showed that even careful counts underestimated actual numbers of burrows by up to 10%. This suggests there are some 11-12,000 burrows, and assuming 90% occupancy, the adult population is likely to be at least 20,000 birds. The population has increased over the last five years, continuing the apparent recovery from a very small population size in the early 20th century. Despite this increase, demographic models indicate that the population remains at risk from relatively small increases in mortality, if mortality is determined primarily by fishing effort. Mitigation of long-line mortality remains the key conservation goal for this species.  相似文献   

A semi-purified test diet for Macrobrachium rosenbergii and the lack of need for supplemental lecithin     

John W. Hilton  Kim E. Harrison  Stanley J. Slinger 《Aquaculture (Amsterdam, Netherlands)》1984,37(3):209-215

Post-larval Macrobrachium rosenbergii were reared for 12 weeks at 27°C in a recirculated aquatic system on semi-purified diet supplemented with between 0 and 10% lecithin. No significant differences were detected in either the weight gain or mortality rates of the prawn reared on the different test diets and no sign of ‘molt death syndrome’ was observed in any of the prawn. It was concluded that supplemental lecithin is not required in the semi-purified diet developed for Macrobrachium rosenbergii when cultured under the experimental conditions of this study. The semi-purified diet formulated in this study would appear to be an adequate test diet to determine many of the qualitative and quantitative nutrient requirements of Macrobrachium rosenbergii. However, the best weight gain of the prawn was on a practical-type diet indicating that further refinement of the test diet formulation may be required.  相似文献