Antifungal activity of <Emphasis Type="Italic">Biscogniauxia</Emphasis> sp. culture filtrates against the rice pathogen <Emphasis Type="Italic">Magnaporthe oryzae</Emphasis>     

Quyet Thi Nguyen  Kana Ueda  Tomoko Tamura  Junichi Kihara  Makoto Ueno 《Journal of General Plant Pathology》2017,83(2):109-112

An ethyl acetate extract of a culture filtrate (ECF) from an unidentified fungal isolate O821 was evaluated for antifungal activity against the rice pathogen Magnaporthe oryzae. The O821-ECF significantly inhibited spore germination, appressorium formation, and mycelial growth of M. oryzae, and its antifungal activity was heat-stable. It also significantly suppressed the number and size of blast lesions. In an analysis of the ITS sequence of this isolate, it shared similarities with species of the fungus Biscogniauxia. These results suggest that isolate O821 of the genus Biscogniauxia produces a heat-stable antifungal compound(s) in its culture filtrate.  相似文献   

Effect of rotifer enrichment with herbal extracts on growth and resistance of red sea bream,Pagrus major (Temminck & Schlegel) larvae against Vibrio anguillarum     

Osamu Takaoka  Seung‐Cheol Ji  Katsuya Ishimaru  Si‐Woo Lee  Gwan‐Sik Jeong  Junichi Ito  Amal Biswas  Kenji Takii 《Aquaculture Research》2011,42(12):1824-1829

The effects of the dietary medicinal herbs Massa medicata, Crataegi fructus (Cf), Artemisia capillaries, Cnidium officinale and their mixture (HM), on growth and resistance against Vibrio anguillarum were examined using larval red sea bream, Pagrus major. The methanol extracts of Cf and HM inhibited the proliferation of seven infectious bacterial strains including the genera Aeromonas, Edwasiella, Photobavterium, Pseudomonas and Vibrio. Rotifers enriched with emulsified bonito oil containing extracts of Cf and HM lowered the bacterial counts in their body on TCBS medium for the genus Vibrio in comparison with rotifers enriched by emulsified bonito oil alone. Although statistically not significant, larvae fed rotifer fortified with Cf from 3 to 20 days after hatching showed the best growth and final body weight followed by those given rotifer with HM and control enrichment. However, final body length of larvae fed rotifer with Cf was significantly larger than larvae fed on rotifers enriched with other herb extracts. After a challenge test with V. anguillarum, survival of larvae fed rotifer with Cf and HM was significantly higher than for the control treatment. These results revealed that Cf and HM in rotifer is useful to promote growth and resistance against V. anguillarum in red sea bream larvae, providing a new technology for mass production of disease‐resistant fry and fingerlings.  相似文献   

Molecular cloning of canine Mcl-1 gene and its expression in tumor cell lines     

Sano J  Oguma K  Kano R  Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(6):709-712

The canine Mcl-1 gene was cloned and sequenced. Canine Mcl-1 clone was 2694 base pairs in length and encoded 350 amino acids. The predicted amino acid sequence was 87.7%, 77.1% and 75.7% homologous to predicted human, mouse and rat Mcl-1, respectively. RT-PCR analysis revealed that canine Mcl-1 mRNA was expressed in PBMCs (peripheral blood mononuclear cells), bone marrow cells, MDCK (Madin-Darby canine kidney) and GL-1 (canine B cell leukemia) whereas undetectable in CL-1 (canine T cell lymphoma) cell line.  相似文献   

Effect of antineoplastic drugs on the expression of Bcl-2 and Bcl-xL genes in the feline T-cell leukemia cell line     

Sano J  Nagafuchi S  Yamazaki J  Oguma K  Kano R  Hasegawa A 《Research in veterinary science》2005,79(3):197-201

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Peculiar Composition of Epithelial Cells in Follicle-Associated Intestinal Crypts of Peyer’s Patches in the Rat Small Intestine     

Youhei MANTANI  Hideto YUASA  Miho NISHIDA  Ei-ichirou TAKAHARA  Takuya OMOTEHARA  Kankanam Gamage Sanath UDAYANGA  Junichi KAWANO  Toshifumi YOKOYAMA  Nobuhiko HOSHI  Hiroshi KITAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(6):833-838

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Construction of a high-density reference linkage map of tea (Camellia sinensis)     

Fumiya Taniguchi  Kazumi Furukawa  Sakura Ota-Metoku  Nobuo Yamaguchi  Tomomi Ujihara  Izumi Kono  Hiroyuki Fukuoka  Junichi Tanaka 《Breeding Science》2012,62(3):263-273

A few linkage maps of tea have been constructed using pseudo-testcross theory based on dominant marker systems. However, dominant markers are not suitable as landmark markers across a wide range of materials. Therefore, we developed co-dominant SSR markers from genomic DNA and ESTs and constructed a reference map using these co-dominant markers as landmarks. A population of 54 F₁ clones derived from reciprocal crosses between ‘Sayamakaori’ and ‘Kana-Ck17’ was used for the linkage analysis. Maps of both parents were constructed from the F₁ population that was taken for BC₁ population. The order of most of the dominant markers in the parental maps was consistent. We constructed a core map by merging the linkage data for markers that detected polymorphisms in both parents. The core map contains 15 linkage groups, which corresponds to the basic chromosome number of tea. The total length of the core map is 1218 cM. Here, we present the reference map as a central core map sandwiched between the parental maps for each linkage group; the combined maps contain 441 SSRs, 7 CAPS, 2 STS and 674 RAPDs. This newly constructed linkage map can be used as a basic reference linkage map of tea.  相似文献   

Evaluation of iron bioavailability from bonito dark muscle using anemic rats     

Matsumoto J  Mori N  Doi M  Kishida T  Ebihara K 《Journal of agricultural and food chemistry》2003,51(15):4478-4482

The bioavailability of iron from ferrous sulfate (FeII-S), heme iron prepared from hemoglobin (HIP), and bonito dark muscle (BDM) was assessed in anemic rats using a hemoglobin regeneration efficiency (HRE) method. Freeze-dried BDM (FD), boiled and freeze-dried BDM (B/FD), and boiled and smoke-dried BDM (B/SD) were used as BDM source. Rats were made anemic by feeding on an iron-deficient diet for 28 days. To replete their iron levels, anemic rats were then fed on a diet containing iron at a level of 17 ppm for 14 days. Rats receiving FeII-S gained significantly more weight and had greater food intake and higher HRE compared to the other four groups. The bioavailability of iron from HIP was poor compared with that from FeII-S and BDM. When the HRE of rats fed FeII-S was 100, that of rats fed BDF was approximately 80. These results suggest that BMD is an effective dietary source of iron.  相似文献   

Characteristics of wastewater treatment using a multi-soil-layering system in relation to wastewater contamination levels and hydraulic loading rates   总被引：2，自引：0，他引：2  

Tsugiyuki MASUNAGA  Kuniaki SATO  Junichi MORI  Matsusige SHIRAHAMA  Hiromoto KUDO  Toshiyuki WAKATSUKI 《Soil Science and Plant Nutrition》2007,53(2):215-223

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干,湿谷混合贮藏干燥的试验研究     

上出顺一  李长友 《华南农业大学学报》1993,14(2):121-126

本文介绍在日本国寺泉干燥中心测得的干湿谷混合干燥试验结果,分析混合干燥设施的能量消耗、干燥效率、物料品质等项技术指标。获得了混合干燥新技术开发的基础性数据。  相似文献   

Immunohistochemical and histoplanimetrical study on the spatial relationship between the settlement of indigenous bacteria and the secretion of bactericidal peptides in rat alimentary tract     

Yokoo Y  Miyata H  Udayanga KG  Qi WM  Takahara E  Mantani Y  Yokoyama T  Kawano J  Hoshi N  Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):1043-1050

To clarify the regulatory mechanism by bactericidal peptides secretion, the secretion of bactericidal peptides was immunohistochemically and histoplanimetrically compared with the degree of Gram-positive/negative bacterial colonization throughout the rat alimentary tract. In the associated exocrine glands from the oral cavity to the stomach, no comparable differences were observed under the changes of development of indigenous bacterial colonies. In the small intestine, immunopositive granules for lysozyme and secretory phospholipase A2 (sPLA2) were markedly decreased, whereas immunopositive vacuoles in the Paneth cells were more increased at sites with hyper-development of indigenous bacterial colonies in the intervillous spaces than at sites with no or less development. No changes in exocrine glands were observed in the large intestine because of the constant existence of large quantities of bacteria. Gram-positive bacterial colonies on the mucosal surfaces were dominant from the oral cavity to the stomach. Gram-negative bacteria were dominant in the large intestine, and the distributions of both Gram-positive and negative bacteria were intermediate in the small intestine. These findings suggest that lysozyme and sPLA2 secreted from the Paneth cells contribute to the regulation of the proliferation of indigenous bacteria in the intervillous spaces of the small intestine, and that the inversion of distributions of Gram-positive and -negative bacteria in the alimentary tract might be caused by the secretion of lysozyme and sPLA2 in the small intestine.  相似文献