Type‐I interferon regulates matrix metalloproteinases clearance of the bovine endometrial spheroid     

Al‐Nur Md. Iftekhar Rahman  Seiya Yamashita  Md. Rashedul Islam  Taisuke Fujihara  Hayato Yamaguchi  Manabu Kawahara  Masashi Takahashi  Hideyuki Takahashi  Takafumi Gotoh  Nobuhiko Yamauchi 《Animal Science Journal》2020,91(1)

This study investigated the effect of type‐I interferon (IFN) on the expression of matrix metalloproteinases (MMPs) of the bovine endometrial stromal cells (BES) and epithelial cells (BEE). The cells were separated and purified from the caruncles and cultured in DMEM/F‐12 containing 10% fetal bovine serum. Spheroids were generated by using ascorbate. Zymograms of the supernatant showed that BEE predominantly expressed MMP‐9, whereas MMP‐2 was expressed in BES and homo‐spheroids. While MMPs expression was not detected in hetero‐spheroids. Real‐time quantitative PCR revealed that type‐I IFN and P4 suppressed the gene expression of MMP‐2 and MMP‐9 in hetero‐spheroids, respectively. On the other hand, gelatin zymography analysis of the supernatant showed that type‐I IFN strongly promote the clearance of MMPs. While zymograms of the MMPs stocked in the hetero‐spheroids were significantly reduced by type‐I IFN. Phenylmethanesulfonyl fluoride and leupeptin (both are serine proteinase inhibitors) significantly repressed the clearance of MMP‐2 and MMP‐9 induced by type‐I IFN. Moreover, collagen fibers in hetero‐spheroids significantly decreased after the treatment with type‐I IFN. In conclusion, it was suggested that type‐I IFN participate in the tissue remodeling by regulation the clearance of MMPs.  相似文献   

Conservation agriculture practice influences soil organic carbon pools in intensive rice-based systems of the Eastern Indo-Gangetic Plain     

Md. Ariful Islam  Richard W. Bell  Chris Johansen  M. Jahiruddin  Md. Enamul Haque  Wendy Vance 《Soil Use and Management》2022,38(2):1217-1236

Studies of rice-based systems in the Indo-Gangetic Plain (IGP) have demonstrated the beneficial effects of Conservation Agriculture on soil organic carbon (SOC) status, along with increased soil health and crop productivity. However, it remains unclear as to the time for such treatments to have a positive effect. In this study of lentil-mung bean-rice and wheat-mung-rice rotations in Bangladesh positive effects of strip planting or bed planting, along with residue return, on SOC pools were apparent after 1.5 years, compared with intensive conventional tillage and limited residue return. Conventional tillage resulted in higher CO₂ emission compared with strip planting or bed planting as did high residue return. In the cereal-dominated rotation, the strip planting system sequestered carbon at a rate of 0.24–0.53 Mg C ha⁻¹ year⁻¹ (at 0–0.15 m depth) while conventional tillage was associated with a carbon loss of 0.52–0.82 Mg C ha⁻¹ year⁻¹. In the legume-dominated rotation, neither practice sequestered SOC. Under strip planting, a minimum annual crop residue input of 1.7 Mg C ha⁻¹ for the cereal-dominated system and 5.2 Mg C ha⁻¹ for the legume-dominated system was required to maintain SOC at equilibrium. We conclude that strip planting with high levels of crop residue return can be an effective and quick strategy in either slowing the loss of SOC or improving C sequestration in the intensive rice-based systems of the Eastern IGP.  相似文献   

山地丘陵区农村居民点空间分布特征及影响机制分析（英文稿）     

王兆林  鄂施璇  杜挺  张露洋  Mofakkarul Islam  李计 《农业工程学报》2022,38(10):277-285

山地丘陵区是受人类活动和自然因素干扰的特殊区域,也是人地关系矛盾突出的地区,是国土空间治理的重点与难点区域。分析山地丘陵区农村居民点的分布特征,对实现乡村重建与乡村振兴具有重要意义。 该研究试图构建研究框架,结合重庆石船镇的案例,从定量和定性两方面探讨山区农村居民点的空间分布特征。结果表明：1）山地丘陵区农村居民点空间分布存在显著差异;农村居民点大多位于低海拔、平缓地形、低灾害风险、离水中等距离、靠近城镇和道路的地区,人口规模、宜居环境等因素影响农村居民点的发展、选址和扩张。2）山地丘陵区高密度地区农村居民点空间分布呈现随机分布、分散分布或均匀分布的特征,而低密度地区农村居民点空间分布呈现聚集分布的特征。 农村居民点更有可能集中在人口密度高的地区,如海拔较低、坡度较缓、距水源中等、地质灾害风险低的地区。3）自然因素、区位因素和社会经济因素相互关联,在不同时期表现出不同的耦合模式,决定了山区农村居民点的数量和分布。随着区域经济社会的发展,自然因素对乡村聚落空间分布的影响逐渐减弱,而区位因素、城镇化、产业发展、交通条件、政府政策等规划因素是改变山区农村居民点空间形态的主导力量。 空间治理的差异性策略是实现山区乡村聚落可持续利用和优化布局的必要条件。  相似文献   

Production of transgenic bovine cloned embryos using piggybac transposition     

Kim S  Saadeldin IM  Choi WJ  Lee SJ  Lee WW  Kim BH  Han HJ  Bang du H  Lee BC  Jang G 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1453-1457

Transgenic research on cattle embryos has been developed to date using viral or plasmid DNA delivery systems. In this study, a different gene delivery system, piggybac transposition, was employed to investigate if it can be applied for producing transgenic cattle embryos. Green or red fluorescent proteins (GFP or RFP) were transfected into donor fibroblasts, and then transfected donor cells were reprogrammed in enucleated oocytes through SCNT and developed into pre-implantation stage embryos. GFP was expressed in donor cells and in cloned embryos without any mosaicism. Induction of RFP expression was regulated by doxycycline treatment in donor fibroblasts and pre-implantational stage embryos. In conclusion, this study demonstrated that piggybac transposition could be a mean to deliver genes into bovine somatic cells or embryos for transgenic research.  相似文献   

Effects of transportation on expression of Hsp90, Hsp70, Hsp27 and αB-crystallin in the pig stomach     

Zhang M  Lv Y  Yue Z  Islam A  Rehana B  Bao E  Hartung J 《The Veterinary record》2011,169(12):312

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Delineating water management zones in a paddy rice field using a Floating Soil Sensing System     

Mohammad Monirul Islam  Timothy Saey  Eef Meerschman  Philippe De Smedt  Fun Meeuws  Ellen Van De Vijver  Marc Van Meirvenne 《Agricultural Water Management》2011,102(1):8

Paddy rice fields are kept inundated during most of the growing period. This requirement is challenging to achieve because of the lack of suitable technologies to detect rapidly percolation prone zones within these fields. The objective of this study was to evaluate a methodology to identify water leakage areas to support precision soil–water management at a within-field level. Therefore, a Floating Sensing System (FloSSy) was designed to record the soil apparent electrical conductivity (EC_a) of a paddy field both under dry and inundated conditions using the electromagnetic induction sensor EM38. Comparison of EC_a data sets obtained under inundated and dry conditions showed that the EC_a measurements under inundated condition (EC_a-i) were more strongly related to soil properties due to the absence of variability in soil moisture and the increased stability of the floating sensing platform. Therefore, we proceeded with the EC_a-i measurements and grouped them into two classes using a fuzzy k-means classification method. These classes showed significant differences in water infiltration: lower EC_a values represented a higher infiltration rate and vice versa. This effect was attributed to differences in soil texture, more specifically the sand content, and its effect on water retention. It was concluded that an EC_a-i survey with FloSSy allowed the detection of soil heterogeneity linked to downward water fluxes which has a potential to support precision soil–water management in inundated fields.  相似文献   

Cropping diversity with rice influences soil aggregate formation and nutrient storage under different tillage systems     

Mohammad M. R. Jahangir  Muhammad Jahiruddin  Hasina Akter  Rahana Pervin  Khandakar Rafiq Islam 《植物养料与土壤学杂志》2021,184(1):150-162

Background : The soils under continuous rice monocropping are currently facing a serious threat of accelerated soil and environmental quality degradation. Aims : Examining the impact of tillage and cropping diversity on soil aggregate stability and associated nutrients in a sub‐tropical rice ecosystem. Methods : A split‐plot experiment with tillage (minimum, MT vs. conventional, CT) as a main plot and cropping diversity [mustard (Brassica napus)–rice (Oryza sativa)–rice (M–R–R), wheat (Triticum aestivum)–rice–rice (W–R–R), and lentil (Lens esculenta)–rice–rice (L–R–R)] as a sub‐plot was repeated for four years. Soil aggregate properties were measured using wet sieving techniques. Soil organic carbon (SOC) and nutrients were measured in different aggregate size groups as well as in the bulk soil samples. Results : Results show that all the aggregate size groups were similar in both MT and CT, except in 0.85–0.30 mm. Likewise, cropping diversities increased soil aggregation, being higher aggregate size of < 0.053 mm in M–R–R relative to the W–R–R and L–R–R, where the latter two were alike. By contrast, > 2 mm aggregates were higher in L–R–R than in M–R–R and W–R–R, where the latter two were similar. The MT increased aggregate mean weight diameter (MWD) by 14% in W–R–R, and by 29% in L–R–R. Soil organic carbon (SOC), total N (TN), and available P were higher in MT than in CT, while it was alike for exchangeable K and available S. While W–R–R had a higher aggregate‐associated SOC, available P, and available S, L–R–R had a higher TN, and M–R–R had a higher exchangeable K. While SOC, TN, and exchangeable K accumulated more in the > 0.85 mm size aggregates, the available P, in contrast, accumulated more in < 0.85 mm size aggregates. Conclusion : Wheat–rice–rice diversity, coupled with minimum tillage, has a higher potential for soil fertility sustenance and crop productivity through better nutrient protection.  相似文献   

Mass spectrometric fingerprints of seed protein for defining Lupinus spp. relationships     

Shahidul Islam  Wujun Ma  Rudi Appels  Guijun Yan 《Genetic Resources and Crop Evolution》2013,60(3):939-952

The Lupinus genus consists of 200–600 species with diversified morphological and biochemical characteristics that provide scope for increasing the value of protein attributes in commercial lupin species through genetic improvement. This study characterised protein polymorphism among 33 selected lupin genotypes representing 19 species from Old World and New World using Matrix-Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) mass spectrometry with the aim of developing a high throughput screen for assessing variation among samples within a genetic resource. MALDI-TOF mass spectrometry analysis recognized 630 polymorphic protein mass peaks. A total of 19 protein mass peaks were identified as species specific and 23 were recognized to be generally common (observed in more than 10 species). A set of 111 rare protein mass peaks common to 2–3 species were identified to uniquely distinguish the respective species in combination with the species specific mass peaks. Overall, the seed protein profiles showed extensive diversity among the species analysed. Phylogenic analysis based on the protein mass peaks clustered the Old World smooth and rough-seeded and New World lupin species separately, in broad agreement with clustering based on morphological characters, life history, cytogenetics, DNA polymorphism and electrophoreses of seed proteins. Two New World lupin species, L. mutabilis Lindl. and L. succulentus Douglas ex K. Koch were grouped into the smooth-seeded Old World group.  相似文献   

Phosphate deficiency signaling pathway is a target of arsenate and phosphate transporter OsPT1 is involved in As accumulation in shoots of rice     

Takehiro Kamiya  Md. Rafiqul Islam  Guilan Duan  Shimpei Uraguchi 《Soil Science and Plant Nutrition》2013,59(4):580-590

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Effects of introduction of common carp, Cyprinus carpio (L.), on the pond ecology and growth of fish in polyculture     

M A Wahab    Z F Ahmed    M Aminul Islam    M S Haq  S M Rahmatullah 《Aquaculture Research》1995,26(9):619-628

The effects of introduction of common carp, Cyprinus carpio (L.). in polyculture with major Indian carps and silver carp were studied in fertilized and fed earthen ponds in Mymensingh, Bangladesh. Bottom-dwelling common carp were found to exert an influence on the quality and quantity of planktonic food organisms. The presence of common carp significantly increased (P < 0.05) the growth of other carps including the bottom-feeder mrigal. Cirrhinus mrigala (Hamilton). A significantly higher fish yield (P < 0.05) was observed in the fish ponds containing common carp when the combined yields of all species in three treatments were compared.  相似文献