2种根结线虫Cg-1及其同源基因的克隆和序列分析     

吴平  卓侃  罗梅  廖金铃 《华中农业大学学报》2013,32(2):36-43

采用同源克隆的方法,首次从能克服Mi-1抗性的象耳豆根结线虫(Meloidog yne enterolobii)中克隆了Cg-1同源基因,同时克隆了一个爪哇根结线虫无毒种群的Cg-1及其同源基因.基因序列比较结果显示,象耳豆根结线虫中不存在Cg-1基因,即象耳豆根结线虫中只具有不包含CAATGA片段的Cg-1同源序列,进一步表明Cg-1可能是一个无毒基因.对Cg-1基因序列分析的结果表明,Cg-1基因可能是通过其第三个开放阅读框(ORF)编码的多肽或结构性mRNA或两者共同行使生物学功能.  相似文献   

椰子废弃物综合利用现状与分析   总被引：1，自引：0，他引：1  

郑侃  梁栋  张喜瑞 《广东农业科学》2013,40(5):175-177

阐述和分析了我国椰子种植概况,椰子在种植以及加工过程中产生的废弃物资源的综合利用现状.其中,种植过程中产生的废弃物包括老椰子树树干、树根、枯萎的椰子叶,椰子花和落裂果,主要用于沼气制取、直燃发电、酿酒、药用和加工建筑材料等;加工过程中产生的废弃物包括椰子水、椰衣、椰壳和椰子粕,主要用于加工饮料、医药制品、活性炭、培养基和饲料等.针对我国椰子废弃物综合利用过程中存在的认识不足、开发程度不深和科研力度不够等问题进行了分析探讨,并提出相应的发展对策.  相似文献   

The suitable harvesting season and the part of moso bamboo (Phyllostachys pubescens) for producing binderless boards     

Yukie Saito  Miyuki Ishii  Masatoshi Sato 《Wood Science and Technology》2013,47(5):1071-1081

The properties of the binderless boards of moso bamboo depending on the harvest seasons and the parts of the height were reported and the optimum harvesting conditions investigated. The binderless boards were prepared from the powdered bamboo harvested each month from June to May, and the parts in height. The hot water extract (HWE), lignin, α-cellulose, and hemicelluloses contents were examined. The board properties were evaluated with internal bonding (IB), water absorption (WA), and thickness swelling (TS). From the experiment, the boards prepared between March and October had higher HWE content and higher IB and lower WA and TS than the ones prepared in other months. The board from the top part showed lower IB and higher WA and TS than the bottom and the middle. The boards prepared from the residue after extraction of HWE showed lower IB than the unextracted samples. These results indicated that for producing board from bamboo, the suitable harvesting season is when HWE contents are higher and that the suitable parts in height for harvesting are the bottom and the middle where lignin contents are not low.  相似文献   

Diversification and genetic differentiation of cultivated melon inferred from sequence polymorphism in the chloroplast genome     

Katsunori Tanaka  Yukari Akashi  Kenji Fukunaga  Tatsuya Yamamoto  Yasheng Aierken  Hidetaka Nishida  Chun Lin Long  Hiromichi Yoshino  Yo-Ichiro Sato  Kenji Kato 《Breeding Science》2013,63(2):183-196

Molecular analysis encouraged discovery of genetic diversity and relationships of cultivated melon (Cucumis melo L.). We sequenced nine inter- and intra-genic regions of the chloroplast genome, about 5500 bp, using 60 melon accessions and six reference accessions of wild species of Cucumis to show intra-specific variation of the chloroplast genome. Sequence polymorphisms were detected among melon accessions and other Cucumis species, indicating intra-specific diversification of the chloroplast genome. Melon accessions were classified into three subclusters by cytoplasm type and then into 12 subgroups. Geographical origin and seed size also differed between the three subclusters. Subcluster Ia contained small-seed melon from Southern Africa and South and East Asia and subcluster Ib mainly consisted of large-seed melon from northern Africa, Europe and USA. Melon accessions of subcluster Ic were only found in West, Central and Southern Africa. Our results indicated that European melon groups and Asian melon groups diversified independently and shared the same maternal lineage with northern African large-seed melon and Southern African small-seed melon, respectively. Cultivated melon of subcluster Ic may have been domesticated independently in Africa. The presence of 11 cytoplasm types in Africa strongly supported African origin of cultivated melon and indicated the importance of germplasm from Africa.  相似文献   

A study of metal oxide on antimicrobial effect of plasma pre-treated cotton fabric     

Y. L. Lam  C. W. Kan  C. W. M. Yuen 《Fibers and Polymers》2013,14(1):52-58

Cotton, a natural fibre that consists of cellulose, is highly popular because it is sweat-absorbing and comfortable to wear. However, cotton fabrics provide an excellent environment for microorganisms to grow, owing to their ability to retain moisture. Therefore, numerous chemicals have been used to enhance anti-microbial activity of cotton textiles. This paper reports results of use of silver oxide (Ag₂O) or zinc oxide (ZnO) as a catalyst in the antimicrobial formulation (halogenated phenoxy compound (Microfresh, MF)) and a binder (Microban, MB) for improved treatment of cotton fabrics and minimisation of side effects of the treatment. In addition, from the morphological study, plasma technology was employed to roughen the surface of the materials to improve loading of metal oxides on the surface. Moreover, the characteristic infra-red bands related to plasma-treated cotton produced results different from untreated fabric, implying plasma treatment can improve hydrophilicity of the fabric. Mechanical strength of the specimens was also increased by plasma treatment. Meanwhile, the research showed that the control fabric slightly inhibited the growth of S. aureus because of the bleach residues on fabric surface. On the other hand, anti-bacterial activity of MF-MB-treated specimen, especially in the presence of metal oxide catalyst, was enhanced, providing a slightly larger zone of inhibition. Moreover, plasma gas contains reactive oxygen species that can enter the cell, eventually causing its death. The hydrophilic nature of carbonyl groups present in oxygen plasma pre-treated specimens also increased the anti-microbial activity after treatment with MF-MB.  相似文献   

Polyester metallisation with electroless silver plating process     

C. W. M. Yuen  S. X. Jiang  C. W. Kan  S. K. A. Ku  P. S. R. Choi  K. P. M. Tang  S. Y. Cheng 《Fibers and Polymers》2013,14(1):82-88

In this paper, electroless silver plating process for polyester was reported. The electroless silver plating is basically divided into four stages including pre-cleaning, sensitisation, electroless silver deposition and post-treatment. As the electroless silver plating stage is the key stage in affecting the brilliant appearance and various functional properties such as conductivity and ultra-violet protection, we will study the effect of process variables, i.e. amount of silver nitrate (AgNO₃), concentration of ammonium hydroxide (NH₄OH), concentration of sodium hydroxide (NaOH) and process temperature, using increased amount of silver in fabric surface as an indicator, for optimising the electroless silver plating process for possible industrial application. Experimental results revealed that amount of silver nitrate (AgNO₃)=1.77×10^?3 mole; concentration of ammonium hydroxide (NH₄OH)=6.25 %; concentration of sodium hydroxide (NaOH)=0.008 g/ml and process temperature=25 °C can give the best electroless silver plating for polyester fabric. The surface characteristics of the electroless silver-plated polyester fabric were evaluated by scanning electron microscope, X-ray photoelectron spectroscopy and X-ray diffraction analysis. Meanwhile, the performance properties of the electroless silver-plated polyester fabric were measured by CIE L*, a* and b* values, conductivity as well as ultraviolet protection. The experimental results would be discussed thoroughly in this paper.  相似文献   

基于小鼠表达谱芯片和比较基因组学分析猪生长发育相关候选基因集     

王琼萍  杨帆  王明辉  何侃  俞沛初  潘玉春 《黑龙江畜牧兽医》2012,(15):145-147

为了在更宽、更深的层面上研究猪生长发育机制,得到以通路为单位的候选基因集以克服传统单基因分析方法的缺点。试验利用模式生物小鼠中的芯片技术,通过基因集富集分析方法(GSEA法)分析基因芯片,确定候选通路。对候选通路成员与猪生长发育相关的数量性状基因座(QTL)数据库进行映射,缩小其作用跨度区域,找出作用的关键基因集。结果表明:得到了56个与猪生长发育相关的候选基因集。  相似文献   

Identification of novel sRNAs involved in oxidative stress response in the fish pathogen Vibrio alginolyticus by transcriptome analysis     

Tao Peng  Jie Kan  Jingsheng Lun  Zhong Hu 《Journal of fish diseases》2019,42(2):277-291

Vibrio alginolyticus as an important pathogen in aquaculture can encounter the oxidative stress produced by the immune system during infection. Previous studies showed that sRNAs have important functions in response to oxidative stress in bacteria; however, less of sRNAs related to oxidative stress response were identified in V. alginolyticus. In this study, a total of 749 novel sRNAs were identified by RNA sequencing; among them, 128 sRNAs were up‐ or downregulated in response to oxidative stress. In addition, 1,870 genes exhibited variation on mRNA levels in oxidative stress response. By analysing the target genes of the sRNAs, we concluded that these sRNAs could regulate expressions of genes responsible for iron transport, catalase, GSH‐dependent defence system, electron transferred and stress response. Moreover, the functions of the sRNAs are also seemed related to the pathogenicity in V. alginolyticus. Based on the results, we constructed the oxidative stress model in V. alginolyticus. This study provides us the first outlook of sRNAs function in oxidative stress response in V. alginolyticus. Furthermore, this study can help us to prevent and control this important opportunistic pathogen in aquaculture.  相似文献   

关键信息基础设施安全策略应用研究     

邢丽平  陈侃  汪璠 《湖北农业科学》2020,59(24):9-15

  相似文献   

Fabrication of PVA-BaSO4 hybrid nanofibers and dispersion of BaSO4 particles via ultrasonic electrospinning     

Hidekazu Sato  Kyu-Oh Kim  Han-Ki Kim  Byoung-Suhk Kim  Yuji Enomoto  Ick-Soo Kim 《Fibers and Polymers》2010,11(8):1123-1127

We reported the preparation and characterization of the poly(vinyl alcohol) (PVA)/BaSO₄ hybrid nanofibers prepared by normal and ultrasonic electrospinning, respectively. Compared to normal electrospinning, BaSO₄ particles in the resultant PVA/BaSO₄ hybrid nanofibers prepared by ultrasonic electrospinning were well-dispersed without severe agglomerations, as confirmed by scanning electron microscopy (SEM) analysis. X-ray diffraction (XRD) analysis indicated that typical crystalline peaks of PVA and BaSO₄ particles were dramatically decreased by ultrasonication during electrospinning. Moreover, the size of BaSO₄ aggregates became smaller.  相似文献