排序方式： 共有107条查询结果，搜索用时 15 毫秒
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Kurakake M Goto T Ashiki K Suenaga Y Komaki T 《Journal of agricultural and food chemistry》2003,51(6):1701-1705
Serratia marcescens YS-1, a chitin-degrading microorganism, produced mainly N-acetylhexosaminidase. The purified enzyme had an optimal pH of approximately 8-9 and remained stable at 40 degrees C for 60 min at pH 6-8. The optimum temperature was around 50 degrees C, and enzyme activity was relatively stable below 50 degrees C. YS-1 N-acetylhexosaminidase hydrolyzed p-nitrophenyl beta-N-acetylgalactosamide by 28.1% relative to p-nitrophenyl beta-N-acetylglucosamide. The N-acetylchitooligosaccharides were hydrolyzed more rapidly, but the cellobiose and chitobiose of disaccharides that had the same beta-1,4 glycosidic bond as di-N-acetylchitobiose were not hydrolyzed. YS-1 N-acetylhexosaminidase efficiently transferred the N-acetylglucosamine residue from di-N-acetylchitobiose (substrate) to alcohols (acceptor). The ratio of transfer to methanol increased to 86% in a reaction with 32% methanol. N-Acetylglucosamine was transferred to the hydroxyl group at C1 of monoalcohols. A dialcohol was used as an acceptor when the carbon number was more than 4 and a hydroxyl group existed on each of the two outside carbons. Sugar alcohols with hydroxyl groups in all carbon positions were not proper acceptors. 相似文献
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Kanako Watanabe Isao Kuriyama Koichi Satoh Viswanath Kiron Shuichi Satoh Takeshi Watanabe 《Fisheries Science》2001,67(1):90-103
SUMMARY: This is another report from a series of studies aimed at determining the energy and protein requirements based on the optimum feeding frequency of yellowtail during the winter season. Experiments were conducted at Mie and Oita Prefectures, Japan, employing extruded pellets of known digestible energy (DE) and digestible protein (DP) values. Four groups of fish were fed at frequencies of five, three, two, and one time(s) per week. The most efficient feed performance was obtained for the feeding frequenecy of three times per week. The DE and DP requirements calculated based on the feeding rate at this frequency were 38.7 kcal and 2.8 g/kgBW per day (14.3–17.3°C) in Mie and 30.4 kcal and 2.2 g/kgBW per day (13.8–17.5°C) in Oita, respectively. The present results and the observations on the changes of the contents in digestive tracts tallied with our previous work, confirming that the optimum feeding frequency was three times per week. 相似文献
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Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant 总被引：4，自引：0，他引：4
Sasaki A Itoh H Gomi K Ueguchi-Tanaka M Ishiyama K Kobayashi M Jeong DH An G Kitano H Ashikari M Matsuoka M 《Science (New York, N.Y.)》2003,299(5614):1896-1898
Gibberellin (GA) regulates growth and development in plants. We isolated and characterized a rice GA-insensitive dwarf mutant, gid2. The GID2 gene encodes a putative F-box protein, which interacted with the rice Skp1 homolog in a yeast two-hybrid assay. In gid2, a repressor for GA signaling, SLR1, was highly accumulated in a phosphorylated form and GA increased its concentration, whereas SLR1 was rapidly degraded by GA through ubiquitination in the wild type. We conclude that GID2 is a positive regulator of GA signaling and that regulated degradation of SLR1 is initiated through GA-dependent phosphorylation and finalized by an SCF(GID2)-proteasome pathway. 相似文献
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Naoko Ohkama-Ohtsu Naoki Kishimoto Junshi Yazaki Fumiko Fujii Kanako Shinbo Zempei Shimatani 《Soil Science and Plant Nutrition》2013,59(5):771-775
Microarray analysis was carried out with mRNAs isolated from aerial portions of rice plants subjected to sulfur deficiency. Among the candidate clones regulated by sulfur deficiency, three genes were identified by northern analysis that showed altered levels of mRNA accumulation. Genes similar to those for ferritin, S-like ribonuclease, and DnaJ-like protein were down-, down-, and upregulated by sulfate deficiency, respectively. These genes were found for the first time to be responsive to sulfur nutrition in the present study. 相似文献
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Hiroko Akiyama Sho Morimoto Kanako Tago Yuko T. Hoshino Kazunari Nagaoka Masatsugu Yamasaki 《Soil Science and Plant Nutrition》2013,59(4):520-529
Nitrous oxide (N2O) is a greenhouse gas that contributes to the destruction of stratospheric ozone, and agricultural soil is an important source of N2O. Aerobic soils are sinks for atmospheric methane (CH4), a greenhouse gas. Ammonia monooxygenase (AMO) can oxidize CH4, but CH4 is mostly oxidized by methane monooxygenase (MMO), and CH4 oxidation by AMO is generally negligible in the soil. We monitored the N2O and CH4 fluxes after urea application in fields containing different soils using an automated sampling system to determine the effects of environmental and microbial factors on the N2O and CH4 fluxes. The soil types were Low-humic Andosol (Gleyic Haplic Andosol), yellow soil (Gleyic Haplic Alisol) and gray lowland soil (Entric Fluvisol). Cumulative N2O emissions from the yellow soil were higher than those from other soil types, although the difference was not significant. The CH4 uptake level by Andosol was one order of magnitude higher than that by other soils. There were significant relationships between the ammonia oxidation potential, AOB and AOA amoA copy numbers, and the CH4 uptake. In contrast, the gene copy numbers of methane-oxidizing bacteria (MOB) pmoA were below the detection limit. Our results suggested that the AMOs of AOB and AOA may have more important roles than those previously considered during CH4 oxidation in agricultural soils treated with N fertilizers. 相似文献
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Kanako Yasuda Takuji Tsukiyama Shanta Karki Yutaka Okumoto Masayoshi Teraishi Hiroki Saito Takatoshi Tanisaka 《Euphytica》2013,192(1):17-24
Miniature Ping (mPing) is the first active miniature inverted-repeat transposable element to be identified in rice, and its mobilization is activated by stress treatments. We have examined the mobilization of mPing in four NERICA (New Rice for Africa) lines and 13 interspecific lines. All 17 lines are inbred progenies derived from crosses between Oryza sativa variety WAB56-104 as the recurrent parent and the O. glaberrima variety CG14 as the donor parent. We found that 16 of the 17 lines studied inherited mPing together with its autonomous partner, Pong, from WAB56-104. Transposon display of mPing disclosed polymorphic banding patterns among these lines. Most importantly, seven of the lines displayed clear polymorphic banding patterns for mPing, indicating that mPing might have been mobilized in these lines. Locus-specific PCR analysis also confirmed the mobilization of mPing. These results signify that interspecific hybridization may activate the transposition of mPing. Based on these results, we discuss the potential use of the mPing system as an efficient tool for gene tagging in interspecific hybrid rice. 相似文献
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Kanako Tsubono Yukio Taniguchi Hirokazu Matsuda Takahisa Yamada Toshie Sugiyama Kosuke Homma Yoshinori Kaneko Satoshi Yamagishi Hiroaki Iwaisaki 《Animal Science Journal》2014,85(4):356-364
Japanese population of the Japanese crested ibis Nipponia nippon was founded by five individuals gifted from the People's Republic of China. In order to exactly evaluate genetic structure, we first performed development of novel genetic makers using 89 microsatellite primer pairs of related species for cross‐amplification. Of these, only three primer pairs were useful for the genetic markers. Additionally, we sequenced allelic PCR products of these three markers together with 10 markers previously identified. Most markers showed typical microsatellite repeat units, but two markers were not simple microsatellites. Moreover, over half of the markers did not have the same repeat units as those of the original species. These results suggested that development of novel genetic markers in this population by cross‐amplification is not efficient, partly because of low genetic diversity. Furthermore, the cluster analysis by STRUCTURE program using 17 markers showed that the five founders were divided into two clusters. However, the genetic relationships among the founders indicated by the clustering seemed to be questionable, because the analysis relied largely on a small number of triallelic markers, in spite of the addition of the three useful markers. Therefore, more efficient methods for identifying large numbers of single nucleotide polymorphisms are desirable. 相似文献