全文获取类型
| 收费全文 | 1313篇 |
| 免费 | 57篇 |
| 国内免费 | 6篇 |
专业分类
| 林业 | 56篇 |
| 农学 | 71篇 |
| 基础科学 | 2篇 |
| 178篇 | |
| 综合类 | 64篇 |
| 农作物 | 37篇 |
| 水产渔业 | 93篇 |
| 畜牧兽医 | 783篇 |
| 园艺 | 11篇 |
| 植物保护 | 81篇 |
出版年
| 2021年 | 25篇 |
| 2020年 | 19篇 |
| 2019年 | 23篇 |
| 2018年 | 23篇 |
| 2017年 | 35篇 |
| 2016年 | 34篇 |
| 2015年 | 38篇 |
| 2014年 | 46篇 |
| 2013年 | 95篇 |
| 2012年 | 82篇 |
| 2011年 | 83篇 |
| 2010年 | 28篇 |
| 2009年 | 38篇 |
| 2008年 | 58篇 |
| 2007年 | 63篇 |
| 2006年 | 81篇 |
| 2005年 | 85篇 |
| 2004年 | 63篇 |
| 2003年 | 60篇 |
| 2002年 | 32篇 |
| 2001年 | 45篇 |
| 2000年 | 52篇 |
| 1999年 | 41篇 |
| 1998年 | 5篇 |
| 1997年 | 9篇 |
| 1996年 | 7篇 |
| 1995年 | 4篇 |
| 1994年 | 4篇 |
| 1992年 | 14篇 |
| 1991年 | 15篇 |
| 1990年 | 16篇 |
| 1989年 | 15篇 |
| 1988年 | 16篇 |
| 1987年 | 18篇 |
| 1986年 | 9篇 |
| 1985年 | 7篇 |
| 1984年 | 5篇 |
| 1983年 | 8篇 |
| 1982年 | 4篇 |
| 1981年 | 4篇 |
| 1979年 | 9篇 |
| 1977年 | 7篇 |
| 1974年 | 5篇 |
| 1973年 | 3篇 |
| 1972年 | 7篇 |
| 1970年 | 3篇 |
| 1968年 | 3篇 |
| 1967年 | 3篇 |
| 1966年 | 3篇 |
| 1965年 | 3篇 |
排序方式： 共有1376条查询结果，搜索用时 750 毫秒
111.
A total of 288 serum samples were collected from 12 species of animals in various localities of Japan from 1975 to 1977. Neutralizing antibody to bovine rotavirus was found in serum samples from all the species, viz., horses, cattle, sheep, goats, pigs, dogs, rabbits, guinea pigs, rats, mice, chickens and human beings. The incidence of neutralizing antibody in titers of 1 : 2 or higher ranged from 31 to 100%, and high incidences exceeding 70% were obtained in horses, cattle, sheep, pigs, dogs, rabbits and human beings. High titers were most common in horses, cattle, sheep and pigs. These serological results suggest that rotaviruses occur commonly in these species of animals. 相似文献
112.
113.
114.
115.
M Asagi T Ogawa T Minetoma K Sato Y Inaba 《American journal of veterinary research》1986,47(10):2161-2164
A reversed passive hemagglutination (RPHA) method was developed for the detection of transmissible gastroenteritis (TGE) virus in the fecal specimens from pigs. Ovine erythrocytes fixed with glutaraldehyde and treated with tannic acid were coated with anti-TGE virus swine antibodies, which were purified by affinity chromatographic technique linked with purified TGE virus. The RPHA test was done by the Microtiter method. Erythrocytes coated with purified specific antibodies were agglutinated by TGE virus, but not by porcine rotavirus or porcine enterovirus. The reaction was specifically inhibited by antiserum against TGE virus, confirming the specificity of the reaction. A litter of seven 3-day-old pigs was orally inoculated with TGE virus, and fecal specimens were obtained once a day and serum was obtained every 4th day. With the RPHA test, TGE virus was detected in the diarrheal feces; all of the inoculated pigs developed virus-neutralization antibody for the TGE virus. The RPHA test detected TGE virus in feces from pigs with naturally occurring diarrhea. The RPHA test detected TGE virus in 5 of 6 fecal specimens (80%), whereas the positive rate was only 50% (3/6) for the immunofluorescent staining of primary cultures of porcine kidney cells inoculated with the specimens. The advantages of the RPHA method are simplicity, high sensitivity, and rapid to do. 相似文献
116.
Nuclear inclusion bodies typical of the adenovirus group were widespread in in the spleen and other tissues of 8-week-old turkeys with severe respiratory disease and concomitant evidence of colisepticemia. Adenoviral virions were seen in affected nuclei of splenic tissue and in negatively stained preparations of ground spleen. In splenic tissue, inclusions were most prominent in reticular cells and macrophages in the periarterial lymphoid sheaths, the red pulp and the marginal zones of the periarteriolar reticular sheaths. Marked reticuloendothelial hyperplasia, lymphoid atrophy and granulocytic splenitis characterized the splenic changes. There were inclusions in the respiratory tract, intestinal tract, liver, kidney and pancreas. Inoculation of young turkeys, especially when immunosuppressed, resulted in evidence of infection and respiratory disease. The viruses produced cytopathic changes in primary turkey kidney cell cultures but did not affect embryonating chicken eggs. Concentrated viral suspensions induced precipitin lines in agar gel immunodiffusion tests with known antisera against known turkey adenoviruses but did not show an antigenic relationship to chicken adenoviruses. 相似文献
117.
118.
119.
120.