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排序方式: 共有438条查询结果,搜索用时 15 毫秒
431.
Mitsuru Oyanagi Kentaro Kaneko Yoshinori Kaneko Maiko Sasaki Chizuko Nishida Yoichi Matsuda Toshiaki Mitsui 《Animal Science Journal》2014,85(8):814-832
We investigated the proteome of a female Crested Ibis (Nipponia nippon, ID#162) that died on March 10, 2010 at the Sado Japanese Crested Ibis Conservation Center. Protein preparations from the brain, trachea, liver, heart, lung, proventriculus, muscular stomach, small intestine, duodenum, ovary and neck muscle were subjected to in‐solution shotgun mass spectrometry (MS)/MS analyses using an LTQ Orbitrap XL mass spectrometer. A search of the National Center for Biotechnology Information Gallus gallus databases revealed 4253 GI (GenInfo Identifier) numbers with the sum of the same 11 tissues examined in the Crested Ibis. To interpret the obtained proteomics data, it was verified in detail with the data obtained from the brain of the Crested Ibis. It has been reported that drebrin A is specifically expressed in adult chicken brain. In the shotgun proteomic analyses of the Crested Ibis, we identified drebrin A as a brain‐specific protein. Furthermore, Western blotting analysis of the protein preparations from 10 tissues of the Crested Ibis and 150‐day‐old hens using anti‐drebrin antibodies showed intensive expression of approximately 110 kDa polypeptides of drebrin in both brains. We believe firmly that the present data will contribute to initial and fundamental steps toward understanding the Crested Ibis proteome. 相似文献
432.
D.H. Shin H. Matsubara S. Kaneko T. Kotani M. Yamashita S. Adachi K. Yamauchi 《Fish physiology and biochemistry》2003,28(1-4):519-520
In order to identify maturational factors that can serve as indicators of egg quality in artificially matured Japanese eel, Anguilla japonica, maturation-promoting factor (MPF; consists of cdc2 and cyclin B) related proteins and chromosomes were examined. Mitogen-activated protein kinase (MAPK; 44 kDa band) and cdc2 (35 kDa and 34 kDa band) were detected. Chromosomes aligned on a vertical spindle appeared normal; however, chromosomes on a horizontal spindle and dispersed chromosomes seemed unusual. 相似文献
433.
S. W. Bang K. Sugihara B. H. Jeung R. Kaneko E. Satake Y. Kaneko Y. Matsuzawa 《Plant Breeding》2007,126(1):101-103
Intergeneric crosses were made between Brassica oleracea and Moricandia arvensis utilizing embryo rescue. Six F1 hybrid plants were generated in the cross‐combination of B. oleracea × M. arvensis from 64 pods by the placenta‐embryo culture technique, whereas three plants were produced in the reciprocal cross from 40 pods by the ovary culture technique. The hybrid plants were ascertained to be amphihaploid with 2n = 23 chromosomes in mitosis and a meiotic chromosome association of (0–3)II + (17–23)I at metaphase I (M I). In the backcross with B. oleracea, some of these hybrids developed sesquidiploid BC1 plants with 2n = 32 chromosomes that predominantly exhibited a meiotic configuration of (9II + 14I) in pollen mother cells. The following backcross of BC2 plants to B. oleracea generated 48 BC3 progeny with somatic chromosomes from 2n = 19 to 2n = 41. The 2n = 19 plants showed a chromosomal association type of (9II + 1I) and a chromosomal distribution type of (91/2 + 91/2) or (9 + 10) at M I and M II, respectively. These facts might suggest that they were monosomic addition lines (MALs) of B. oleracea carrying a single chromosome of M. arvensis that could offer potential for future genetic and breeding research, together with other novel hybrid progeny developed in this intergeneric hybridization. 相似文献
434.
Genetic stability and maintenance of Raphanus sativus lines with an added Brassica rapa chromosome 总被引:2,自引:0,他引:2
The genetic stability and maintenance of Raphanus sativus‐Brassica rapa monosomic chromosome addition lines (a‐h‐types MALs, 2n = 19, BC2), developed by backcrossing the synthesized amphidiploid Raphanobrassica (Raphanus sativus × Brassica rapa, 2n = 38, RRAA) with R. sativus cv. ‘Shogoin’ (2n = 18, RR), was investigated. Transmission of the added alien chromosome through selected smaller seeds (SSS) and the inheritance of morphological traits and random amplified polymorphic DNA (RAPD)‐specific markers together with meiotic chromosome configuration and seed fertility were also investigated for three successive generations (BC3 to BC5). The distinctive traits and the RAPD‐specific markers of the eight types of MAL were substantially inherited and stably maintained throughout three generations, although a few variant plants (2n =18) resembling MALs (2n = 19) and hyperploidal plants (2n = 26 and 2n = 37) were generated in the earlier generations of BC3 and BC4 in comparison with BC5. The average transmission rates for three generations ranged from 26% for both the b‐type and the d‐type to 44% for the e‐type through SSS. On the other hand, the transmission rates through randomly selected seeds (RSS) were lower, ranging from 6.5% for the f‐type to 23.5% for the b‐type. In meiosis, more than 90% of PMCs showed the 9II +1I pairing configuration at metaphase I throughout three generations. For seed fertility, when backcrossed with the radish cv. ‘Shogoin’, the values were approximately 180% to 500% with the mode around 300% with the seed harvested from a pod increasing with the advancing generations. Genetic recombination between the radish chromosomes and the added chromosome is probably rare, suggesting that the added chromosome is mostly maintained unaltered in the background of the radish genome. 相似文献
435.
436.
Thyroid-stimulating hormone and prostaglandin E1 stimulation of cyclic 3',5'-adenosine monophosphate in thyroid slices 总被引:8,自引:0,他引:8
Thyroid-stimulating hormone increased the cyclic 3',5'-adenosine monophosphate concentration in dog thyroid slices during a 1-minute incubation period and produced a maximum effect soon thereafter. The elevation persisted for at least 30 minutes. The concentrations of the cyclic 3',5'-adenosine monophosphate increased as the TSH concentration was increased from 0.125 to 50 milliunits per milliliter. Prostaglandin E(1), which increases glucose oxidation in dog thyroid slices, also increased the concentration of cyclic 3',5'-adenosine monophosphate. Although sodium fluoride stimulates thyroid adenyl cyclase, it did not increase concentration of cyclic 3',5'-adenosine monophosphate. Carbamylcholine and menadiol sodium diphosphate augment glucose oxidation in dog thyroid slices but do not change concentrations of cyclic 3',5'-adenosine monophosphate. 相似文献
437.
Takeshi Yamamoto Hiroyuki Matsunari Tsuyoshi Sugita Hirofumi Furuita Toshiro Masumoto Yasuro Iwashita Shunji Amano Nobuhiro Suzuki 《Fisheries Science》2012,78(2):359-366
To minimize the supplemental essential amino acids (EAAs) to a fish meal-free diet for rainbow trout Oncorhynchus mykiss, four types of fish meal-free diets and a control fish meal-based diet were fed to triplicate groups of trout (initial BW,
16 g) for 10 weeks. Two fish meal-free diets based on a fermented and an unfermented soybean meal were unsupplemented with
EAAs (diet FSBM and SBM), and two fish meal-free diets based on the fermented soybean meal were supplemented with a combination
of lysine and methionine (diet F + ML) and all EAAs (diet F + All), the EAA contents of which were lower than those of the
control diet (diet FM). Although physiological conditions such as the biliary bile acid status and morphological features
of fish fed diet FSBM were improved and similar to those of fish fed diet FM, the growth performance was not significantly
different (P > 0.05) from fish fed diet SBM. The growth performances and whole body protein contents of fish fed F + ML and F + All diets
were similarly improved compared to fish fed diet FSBM. These findings indicate that supplementation of lysine and methionine
to a fermented soybean meal-based fish meal-free diet is enough to maximize the amino acid utilization of the diet. 相似文献
438.