A characterization of the soils and sediments in contaminated sites and rivers using petroleum biomarker compounds     

Kao  Nien-Hsin  Su  Ming-Chien  Yen  Chih-Chun  Huang  Yu-Jen 《Journal of Soils and Sediments》2019,19(1):241-254

Journal of Soils and Sediments - The objective of this study is to reveal the presence of petroleum biomarkers in sediments of contaminated sites and to prove the existence of these compounds in...  相似文献   

种公鸡的饲养与选择     

Fred H．Kao 《中国家禽》2008,30(10):36-37

1 种公鸡的管理目标 为提高种公鸡的利用率与孵化率,良好的种公鸡管理不可缺少,种公鸡的管理目标包括:提高下代孵化率与整齐度;采取良好的农场管理与生物安全措施以尽量增加下代总数.  相似文献   

牛磺酸对黄瓜幼苗生长和生理特性的影响   总被引：5，自引：1，他引：4  

郝林华  陈靠山  李光友 《上海交通大学学报(农业科学版)》2005,23(1):10-14

采用牛磺酸溶液培育黄瓜幼苗,牛磺酸浓度分别为10、100、500、1000、5000mg·L-1,测定牛磺酸对黄瓜幼苗生长指标和生理特性指标包括光合作用PSⅡ的光化学效率、细胞膜相对透性和膜脂过氧化的影响。结果表明,适量浓度的牛磺酸处理可明显促进黄瓜幼苗的生长、增强幼苗的素质和促进壮苗的形成,并在一定程度上提高黄瓜幼苗叶片的光化学效率,降低细胞膜相对透性和膜脂过氧化产物的含量,最适处理浓度约为500mg·L-1,说明牛磺酸对黄瓜幼苗细胞膜有一定的保护作用。  相似文献   

Natural Product Chemistry of Gorgonian Corals of the Family Plexauridae Distributed in the Indo-Pacific Ocean     

Li-Hsueh Wang  Jyh-Horng Sheu  Shih-Yao Kao  Jui-Hsin Su  Yung-Husan Chen  Yu-Hsin Chen  Yin-Di Su  Yu-Chia Chang  Lee-Shing Fang  Wei-Hsien Wang  Yang-Chang Wu  Ping-Jyun Sung 《Marine drugs》2012,10(11):2415-2434

The structures, names, bioactivities and references of 105 natural products obtained from gorgonian corals belonging to the family Plexauridae with an Indo-Pacific distribution are described in this review. All compounds mentioned in this review were obtained from gorgonian corals belonging to the genera Astrogorgia, Bebryce, Echinomuricea, Euplexaura and Menella.  相似文献   

Collaborative non-self recognition system in S-RNase-based self-incompatibility   总被引：6，自引：0，他引：6  

Kubo K  Entani T  Takara A  Wang N  Fields AM  Hua Z  Toyoda M  Kawashima S  Ando T  Isogai A  Kao TH  Takayama S 《Science (New York, N.Y.)》2010,330(6005):796-799

Self-incompatibility in flowering plants prevents inbreeding and promotes outcrossing to generate genetic diversity. In Solanaceae, a multiallelic gene, S-locus F-box (SLF), was previously shown to encode the pollen determinant in self-incompatibility. It was postulated that an SLF allelic product specifically detoxifies its non-self S-ribonucleases (S-RNases), allelic products of the pistil determinant, inside pollen tubes via the ubiquitin-26S-proteasome system, thereby allowing compatible pollinations. However, it remained puzzling how SLF, with much lower allelic sequence diversity than S-RNase, might have the capacity to recognize a large repertoire of non-self S-RNases. We used in vivo functional assays and protein interaction assays to show that in Petunia, at least three types of divergent SLF proteins function as the pollen determinant, each recognizing a subset of non-self S-RNases. Our findings reveal a collaborative non-self recognition system in plants.  相似文献   

Self-organizing map network for automatically recognizing color texture fabric nature     

Chung-Feng Jeffrey Kuo  Chih-Yuan Kao 《Fibers and Polymers》2007,8(2):174-180

The method of recognizing color texture brought forth in the present study is to employ unsupervised learning network to automatically recognize the fabric type and the main texture types. Firstly, the color scanner is adopted to extract fabric image which is afterwards saved as the digital image. Secondly,CIE-Lab color model is taken to obtain the feature value and wavelet transform is utilized to display the texture of the fabric image. Thirdly, co-occurrence matrix is employed to figure out the feature values of the texture structure such as angular second moment, entropy, homogeneity, contrast. Finally, self-organizing map (SOM) network is used as the classifier. The experiment result shows that the study can automatically and accurately classify the fabric types (including shuttle-woven fabric, jersey fabric and non-woven fabric) and main texture type of the fabric (such as plain weave, twill weave, satin weave, single jersey, double jersey and non-woven fabric).  相似文献   

Purification and characterization of a novel extracellular tripeptidyl peptidase from Rhizopus oligosporus     

Lin JS  Lee SK  Chen Y  Lin WD  Kao CH 《Journal of agricultural and food chemistry》2011,59(20):11330-11337

A novel extracellular tripeptidyl peptidase (TPP) was homogenously purified from the culture supernatant of Rhizopus oligosporus by sequential fast protein liquid chromatography. The purified enzyme was a 136.5 kDa dimer composed of identical subunits. The effects of inhibitors and metal ions indicated that TPP is a metallo- and serine protease. TPP was activated by divalent cations, such as Co(2+) and Mn(2+), and completely inhibited by Cu(2+). Enzyme activity was optimal at pH 7.0 and 45 °C with a specific activity of 281.9 units/mg for the substrate Ala-Ala-Phe-pNA. The purified enzyme catalyzed cleavage of various synthetic tripeptides but not when proline occupied the P1 position. Purified TPP cleaved the pentapeptide Ala-Ala-Phe-Tyr-Tyr and tripeptide Ala-Ala-Phe, confirming the TPP activity of the enzyme.  相似文献   

Using Seven Types of GM (1, 1) Model to Forecast Hourly Particulate Matter Concentration in Banciao City of Taiwan     

Tzu-Yi Pai  Ching-Lin Ho  Shyh-Wei Chen  Huang-Mu Lo  Pao-Jui Sung  Shu-Wen Lin  Wei-Jia Lai  Shih-Chi Tseng  Shu-Ping Ciou  Jui-Ling Kuo  Jing-Tang Kao 《Water, air, and soil pollution》2011,217(1-4):25-33

In this study, seven types of first-order and one-variable grey differential equation model (abbreviated as GM (1, 1) model) were used to predict hourly particulate matter (PM) including PM₁₀ and PM_2.5 concentrations in Banciao City of Taiwan. Their prediction performance was also compared. The results indicated that the minimum mean absolute percentage error (MAPE), mean squared error (MSE), root mean squared error (RMSE), and maximum correlation coefficient (R) was 14.10%, 25.62, 5.06, and 0.96, respectively, when predicting PM₁₀. When predicting PM_2.5, the minimum MAPE, MSE, RMSE, and maximum R value of 15.24%, 11.57, 3.40, and 0.93, respectively, could be achieved. All statistical values revealed that the predicting performance of GM (1, 1, x ⁽⁰⁾), GM (1, 1, a), and GM (1, 1, b) outperformed other GM (1, 1) models. According to the results, it revealed that GM (1, 1) GM (1, 1) was an efficiently early warning tool for providing PM information to the inhabitants.  相似文献   

台湾西南部优势竹类箣竹根力之研究   总被引：5，自引：2，他引：3  

高齐治  张俊斌  张新萍 《世界竹藤通讯》2008,6(1):10-15

箣竹为台湾西南部泥岩地区早期主要造林树种,约占该地区绿蔽率之80%,形成极优势之植物。近年来因竹材价格低落,竹林之经营管理减少,其更新造林及其对坡面之保育功能之评价渐受重视。因此本研究为针对箣竹其根力特性,特进行根系与根域范围调查、根段拉力与植株引拔试验等,推估其坡面保育功效。研究结果为箣竹之根段拉力强度(Y)与其直径(X)成指数正相关,而箣竹之引拔抗力(F)与其胸高直径(D)成指数正相关。  相似文献   

人CD14信号肽介导卵清蛋白在HEK293T细胞的高效表达     

能昌爱  李秀锦  仲飞  李振  张峰  陈慧慧  张考  韩冬梅 《动物营养学报》2012,24(11):2165-2171

本试验将人CD14信号肽序列与卵清蛋白（ovalbumin,OVA）基因融合,旨在提高OVA在非输卵管上皮细胞中的表达。提取鸡输卵管上皮细胞总RNA,经反转录合成的cDNA,再利用修饰的特异性引物,通过PCR从cDNA中分别扩增出5’端与人CD14信号肽序列融合和非融合的OVA基因,并将它们分别插入到真核表达载体pcDNA3.1A中,构建成表达载体pcDNA-CD14sp-OVA和pcDNA-OVA。经磷酸钙介导,将质粒转染至HEK293T细胞,使其进行表达,用鼠抗OVA单克隆抗体通过免疫印迹（Western blot）法检测OVA表达水平。结果表明:本试验扩增的OVA基因序列与GenBank中的序列（登录号NM₂05152）一致,构建的人CD14信号肽序列融合OVA基因的表达载体结构正确。经过在HEK293T细胞的表达,融合人CD14信号肽序列的重组蛋白OVA的表达水平明显得到提高。结果提示,融合人CD14信号肽序列后的重组蛋白OVA在非输卵管上皮细胞中的表达水平明显提高,为OVA基因作为DNA疫苗模式抗原的应用提供了必要的条件。  相似文献