Transgenic cell lines which stably express progestogen receptors (PRS) α and β and the PR-responsive reporter genes     

T. Ikeuchi  T. Kobayashi  T. Todo  Y. Nagahama 《Fish physiology and biochemistry》2003,28(1-4):151-151

Transgenic cell lines which stably express progestogen receptors (PRs) and the PR-responsive reporter genes were developed. They are a good system for rapid, sensitive and reproducible screening of various ligands.  相似文献   

Neurotoxins in a toxic red tide of Heterosigma akashiwo (Raphidophyceae) in Kagoshima Bay, Japan   总被引：3，自引：0，他引：3  

S Khan  O Arakawa  Y Onoue 《Aquaculture Research》1997,28(1):9-14

In April 1995, an unusual large-scale red tide of Heterosigma akashiwo (Hada) Hada occurred, causing massive fish kills in Kagoshima Bay, Japan. Four neurotoxic components, HaTx-i , HaTx-ii a, HaTx-ii b and HaTx-iii , which corresponded to brevetoxin components. PbTx-2. PbTx-9, PbTx-3 and oxidized PbTx-2, were inferred from analysis of Heterosigma red tide toxins on TLC and HPLC.  相似文献   

The size composition of the eggs of curimbatá, Prochilodus scrofa Steindachner 1881, induced to spawn with human chorionic gonadotropin (HCG)     

Nelsy Fenerich-Verani  Heloisa M. Godinho  Massuka Y. Narahara 《Aquaculture (Amsterdam, Netherlands)》1984,42(1):37-41

Curimbatá, Prochilodus scrofa, were examined and selected for induced spawning with human chorionic gonadotropin (HCG). Intra-ovarian eggs were sampled and size composition was analyzed.With 21 females, two doses of HCG were applied at 10-h intervals, 5 IU and 10 IU per gram of total weight. According to response, three distinct groups were identified. For the groups where eggs were successfully fertilized, followed by embryonic development, the percent frequency distribution of egg diameter was unimodal with a constant mode of 1111.15 μm. It is suggested that for P. scrofa, selection of recipient females can be made on egg frequency-diameter distribution.  相似文献   

Effects of dietary thiamin on the physiological status of the grouper <Emphasis Type="Italic">Epinephelus coioides</Emphasis>     

J. W. Huang  L. X. Tian  Z. Y. Du  H. J. Yang  Y. J. Liu 《Fish physiology and biochemistry》2007,33(2):167-172

This study was conducted to evaluate the effects of dietary thiamin on the physiological status of the juvenile grouper, Epinephelus coioides. Graded levels of thiamin (0.08, 0.50, 2.12, 3.15, 4.63, 12.37 mg thiamin kg⁻¹ diet) were fed to grouper juveniles (mean weight: 16.97 ± 0.14 g) for 10 weeks. Although fish fed the thiamin-deficient (TD) diet showed no obvious symptoms of thiamin deficiency or increased mortality, those fed the lowest doses of thiamin (0.08 and 0.50 mg thiamin kg⁻¹ diet) had significantly decreased transketolase activity in the liver. In addition, the level of liver thiobarbituric acid reactive substances in fish fed the TD diet was 33–67% higher than that in fish with the thiamin-supplemented diet. There were no significant differences in superoxide dismutase activity between the different groups of fish.  相似文献   

Effect of dietary iron supplement on growth, haematology and microelements of juvenile grouper, Epinephelus coioides   总被引：1，自引：0，他引：1  

C.-X. YE  Y.-J. LIU  K.-S. MAI  L.-X. TIAN  H.-J. YANG  J. NIU  & J.-W. HUANG 《Aquaculture Nutrition》2007,13(6):471-477

An experiment was conducted to investigate the effect of dietary iron supplement on growth, haematology and microelements of juvenile grouper, Epinephelus coioides. Casein–gelatine‐based diets supplemented with 0, 50, 100, 150, 200 and 250 mg kg⁻¹ iron from ferrous sulphate were fed to grouper (mean initial weight: 21.0 ± 0.2 g) for 8 weeks. Weight gain was highest in fish fed the diet supplemented with 100 mg kg⁻¹ iron, intermediate in fish fed diets with 50, 150, 200 and 250 mg kg⁻¹ iron and lowest in fish fed the basal diet. Feed efficiency followed a similar trend except that the lowest value was in fish fed the basal diet and the diet supplemented with 250 mg kg⁻¹ iron. Hepatic iron was highest in fish fed diets supplemented with iron ≥100 mg kg⁻¹, followed by fish fed diet with 50 mg kg⁻¹ iron and lowest in fish fed the basal diet. The whole‐body iron was lowest in fish fed the basal diet but not significantly different from other groups, as judged by anova . Iron supplement to the basal diet had no significant effect on haematological parameters (red blood cell count, haematocrit and haemoglobin), hepatic copper concentration or manganese, zinc concentration in liver and whole body. Broken‐line analysis of hepatic iron indicated that iron supplementation of 100 mg kg⁻¹ satisfied the hepatic iron storage and that further supplementation did not expand the iron status.  相似文献   

Cascading effects of introduced Nile perch (Lates niloticus) on the foraging ecology of Nile tilapia (Oreochromis niloticus)     

G. N. Bwanika  L. J. Chapman  Y. Kizito  J. Balirwa 《Ecology of Freshwater Fish》2006,15(4):470-481

Abstract –  Nile tilapia ( Oreochromis niloticus L.) is the dominant of the introduced tilapiines in many East African lakes and has flourished in the presence of introduced Nile perch ( Lates niloticus L.). We explored the hypothesis that O. niloticus exhibits increased omnivory in response to a decline in abundance of haplochromine cichlids. First, we quantified variation in habitat use and diet of O. niloticus in Lake Nabugabo, Uganda. Second, we compared the diet of O. niloticus in lakes with (Nabugabo, Victoria) and without (Mburo, Wamala, Nyamusingiri, Kyasanduka) introduced Nile perch. In Lake Nabugabo, a higher level of phytoplanktivory was observed in small juveniles than in larger fish and in wetland ecotone areas where haplochromines were most abundant. An omnivorous diet dominated by detritus and invertebrates was recorded for O. niloticus in lakes Nabugabo and Victoria, while a predominantly herbivorous diet was characteristic of O. niloticus in lakes without Nile perch. Availability of a broad food base in lakes where inshore insectivores have been reduced may explain the increased omnivory recorded in lakes Nabugabo and Victoria.  相似文献   

Allozyme variation in riverine and lacustrine populations of Macrobrachium nipponense (de Haan)     

J. T. Y. WONG  B. J. McANDREW 《Aquaculture Research》1994,25(4):393-400

Abstract. Allozyme variation at 32 protein genetic loci was studied in four populations of Macrobrachium nipponense (de Haan) from two contrasting habitats, wholly freshwater lacustrine sites and brackish water riverine areas from different parts of Asia (Hong Kong, Canton, Wuhan, Japan). The average values of observed heterozygosity were 7·45% for lacustrine and 12·30% for riverine sites, the percentage of polymorphic loci (0·99) was 32·5%. The freshwater/lacustrine populations had lower estimates of genetic variability than the river/brackish water populations. The average genetic similarity and genetic distance (Nei's) were 0·92 and 0·084 respectively. The results were compared with those of the only other study on this species. The implications of these results for this species are discussed.  相似文献   

Adaptations versus accommodations: some neuroendocrine aspects in teleost fish     

Y. -A. Fontaine 《Fish physiology and biochemistry》1993,11(1-6):147-154

  相似文献   

Apparent digestibility coefficient of protein sources for carp, Cyprinus carpio L.     

G Degani  S Viola  Y Yehuda 《Aquaculture Research》1997,28(1):23-28

The digestibility of various diet components as protein sources for common carp, Cyprinus carpio L., at 500–800 g was studied by using an inert marker in the feed and collecting the faeces by stripping. Protein in fish meal (83.8%) and soybean meal (69.8%) were significantly more digestible than that of poultry meal (47.2%). Energy digestibility tests gave the following results: fish meal 93.4%, soybean meal 74.7% and poultry meal 63.9%. It was evident that the ability of carp to digest proteins is very high, and that they can utilize proteins as the main component of their diet.  相似文献   

Gustatory sensitivity of the external taste buds of Oreochromis niloticus L. to amino acids     

S Y Yacoob  K Anraku  T Marui  T Matsuoka  G Kawamura  & M Vazquez Archdale 《Aquaculture Research》2001,32(3):217-222

The gustatory sensitivity of the Nile tilapia, Oreochromis niloticus L., to different amino acids was studied using an electrophysiological approach. The electrical responses were recorded from a branch of the facial nerve innervating the external taste buds of the upper lip. The relative stimulatory effectiveness (RSE) of nine amino acids and betaine were determined at a concentration of 1 mm and all of them elicited neural responses. This species responded well to the neutral, basic and acidic amino acids. The most stimulatory amino acids were L‐histidine, L‐arginine, L‐serine, L‐methionine and L‐glutamine; L‐proline and betaine were the least stimulatory. The results of this study suggest that the Nile tilapia has high external gustatory sensitivity to some amino acids as a physiological adaptation to search effectively for their sources. The effect of the pH, ranging from 4.0 to 9.0, on the RSE of three neutral amino acids and artificial pond water (APW) was also studied. The RSE increased below pH 6.0 and was relatively unaffected from 7.0 to 9.0, indicating that acidified stimulants are highly stimulatory in this species. Nile tilapia did not discriminate the pH of APW as effectively as some of the species studied earlier.  相似文献