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In a search for alternative, environmentally friendly and effective disinfecting agents, a commercially available protease—Neutrase®—was tested in this work for inactivation of koi herpesvirus (KHV) and of viral haemorrhagic septicaemia virus (VHSV). For comparison, the stability of these viral pathogens in similar configurations at various pH values and concentrations of peracetic acid or quicklime, typically used for disinfection, was tested. Therefore, virus suspensions were incubated with various concentrations of different agents for 24 hr and the titre of the remaining infectious particles was determined by virus titration. Furthermore, the treatment of both viruses, with the agents at concentrations that were previously appointed as effective, was also examined in the presence of solid material (quartz sand). All procedures investigated in this study, including the protease treatment, were able to reduce the titre of KHV and VHSV below the detection limit of the titration. Although further studies are necessary, this is the first report of the application of a protease for the inactivation of the selected fish pathogens, demonstrating the great potential of the latter for disinfection.  相似文献   
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To improve the storability of sugar beets, this study aimed at determining reasons for genotypic variability in sugar losses and invert sugar accumulation during storage, and at identifying indirect criteria to select for varieties with low storage losses prior to storage. In 2011 and 2012, 18 genotypes, and in 2012 and 2013, six genotypes cultivated at two locations were stored for 8 and 12 weeks at 8°C under controlled conditions. The same 18 genotypes were grown under stress conditions in Spain in 2012/2013. Sugar losses were closely correlated with the invert sugar accumulation after storage. Genotypic differences in storage losses were primarily caused by differences in the level of infestation with microorganisms. The invert sugar accumulation was lower for genotypes with high marc concentration before storage, pointing to a non‐specific resistance. Additionally, the sugar concentration in dry matter before storage, and the invert sugar concentration after cultivation under stress conditions correlated with the invert sugar concentration after storage. These parameters are therefore suggested as criteria to select for improved storability of sugar beet genotypes.  相似文献   
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The emergence and spread of infectious diseases in mid-latitudes, so far mainly observed in the tropics, considerably increase under the current situation of climate change. A recent example is the Usutu virus (USUV) outbreak in Austria. USUV is closely related to the West Nile virus in the U.S. and caused mass mortalities mainly of blackbirds (Turdus merula). The USUV flavivirus persists in a natural transmission cycle between vectors (mosquitoes) and host reservoirs (birds) and leads - once endemic in a population - to periodic outbreaks. In an epidemic model to explain the USUV dynamics in Austria 2001-2005, USUV dynamics were mainly determined by an interaction of bird immunity and environmental temperature. To investigate future scenarios, we entered temperature predictions from five global climate models into the USUV model and also considered four different climate-warming scenarios defined by the I ntergovernmental Panel on Climate Change, IPCC (20 different model-scenario combinations). We downscaled the 20 time series of predicted temperatures (through the year 2100) to represent the region around Vienna. Our simulations predict that USUV will persist in the host population after the epidemic peak observed in 2003. USUV-specific annual blackbird-mortality time series predict that the outbreak frequency increases successively from the beginning to the end of the century. Simulations of worst-case scenarios result in an endemic equilibrium with a decline of the blackbird population of about 24%. Additionally we calculated the annually averaged basic reproduction number for the period 1901-2100. The latter depict that undetected major outbreaks before 2000 were unlikely, whereas it is likely that the USUV becomes endemic after 2040.  相似文献   
36.
We conducted a longitudinal study to follow-up the anti-Neospora caninum serologic status in 30 initially seropositive and 83 initially seronegative cows during their pregnancy. Study cows were blood-sampled every other month during pregnancy until parturition. Blood serum samples were screened for anti-N. caninum antibodies by ELISA. Cows that seroconverted were re-tested by immunoblot as a confirmation test. Among 30 seropositive cows, 28 cows remained seropositive during the whole pregnancy, whereas 2 cows transiently tested negative at least once during pregnancy. Among 83 seronegative cows, 82 cows remained seronegative and 1 cow tested positive three times during the sixth, eighth and last month of pregnancy. As only 2 out of 30 seropositive animals and 1 out of 83 animals changed their serologic status during pregnancy, the study results indicate that there is only a minor temporal instability of anti-N. caninum antibody reactivity in adult cattle.  相似文献   
37.
Priming: it’s all the world to induced disease resistance   总被引:2,自引:0,他引:2  
After infection by a necrotising pathogen, colonisation of the roots with certain beneficial microbes, or after treatment with various chemicals, many plants establish a unique physiological situation that is called the ‘primed’ state of the plant. In the primed condition, plants are able to ‘recall’ the previous infection, root colonisation or chemical treatment. As a consequence, primed plants respond more rapidly and/or effectively when re-exposed to biotic or abiotic stress, a feature that is frequently associated with enhanced disease resistance. Though priming has been known as a component of induced resistance for a long time, most progress in the understanding of the phenomenon has been made over the past few years. Here we summarize the current knowledge of priming and its relevance for plant protection in the field.  相似文献   
38.
Winter triticale, an abundant biomass source for producing biogas in Central Europe, has been bred until now for increased grain yield but not for early biomass yield. Therefore, our objectives were to assess the feasibility of breeding triticale for simultaneous improvement of early biomass and grain yield as well as to predict the early biomass yield using an index of traits measured at maturity stage. A representative sample of 100 triticale lines was evaluated in Experiment I at four locations in Germany. Traits were measured at two growth stages (early dough and full maturity). Genetic variances were significantly different from zero for all traits. The correlated response for early biomass yield estimated from grain yield (0.53) was high, which points to the efficiency of indirect selection. A multiple regression model was developed to predict early biomass yield. The reliability of the model was confirmed in an independent Experiment II with 49 lines. The calibration model explained 55% of total variation for early biomass yield. This clearly underlines that at least during earlier stages of selection, field testing can be carried out for grain yield and related traits to select potential genotypes with high biomass yield based on the developed regression model.  相似文献   
39.
The horticultural sector has seen much structural change both nationally and internationally over the last decades, but the implications for plant health have been neglected. We review in the context of the risk of emerging plant diseases recent developments including the movement towards a global horticultural market, the rise of the horticultural industry of many developing countries, and the economic integration of the European Union. North America is typically well ahead of other regions in economic developments, and in horticulture this is shown for example by the growing importance of Mexican growers. Asia is rapidly catching up also in horticulture, with China and India becoming key producers. Australia and New Zealand show the impact of change in horticulture extension services. The Eastern enlargement of the EU is having profound influences on fruit and vegetable growers both in the new and in the old member countries. Similar developments are taking place in South America and Africa. In all continents, there is a general trend towards fewer and larger horticultural growers, an increasing role of supermarkets and a concentration of the retail pathways. These developments have consequences for the control of plant pathogens and invasive species. Technical issues seem to be of lesser consequence in terms of structural change compared with labour and trade aspects. However, examples can be found where technical innovations have opened up new opportunities or provided solutions to pressing problems, as can be seen in the hardy nursery stock and ornamental industry in the UK. Future technical, economic and social impacts on the sector are likely to play a key role for securing a diverse and reliable food supply for the still expanding world's population. Recent advances in modelling disease spread in complex networks representing trade pathways should be used to target control of introductions of new plant pathogens. There is a need for more long-term research on how structural change in the horticultural sector will affect and be affected by climate change.  相似文献   
40.
Vascular wilt caused by the soil-borne fungus Verticillium dahliae is a major yield and quality-limiting disease across a broad spectrum of crop plants worldwide. Sulphur-enhanced plant defence mechanisms provide an opportunity to effectively and environmentally safely constrain the wilt disease levels in planta. To evaluate the influence of sulphur nutrition on the protective potential of these mechanisms, two near-isogenic tomato genotypes differing in fungal susceptibility, were treated with low or supra-optimal sulphur supply. Microscopic analysis revealed a significant sulphur-induced decrease in the amount of infected vascular cells in both genotypes. However, plant shoot and severely pathogen-affected root growth did not display this distinct alleviating influence of sulphur nutrition. Rates of leaf photosynthesis were impeded by Verticillium dahliae infection in both genotypes especially under low sulphur nutrition. However, assimilate transport rates in the phloem sap were enhanced by fungal infection more in the resistant genotype and under high sulphur nutrition suggesting a stronger sink for assimilates in infected plant tissues possibly involved in sugar-induced defence. A SYBR Green-based absolute quantitative Real-Time assay using a species-specific primer was developed which sensitively reflected sulphur nutrition-dependent changes in fungal colonization patterns. High sulphur nutrition significantly reduced fungal spread in the stem in both tomato genotypes. Concentrations of selected sulphur-containing metabolites revealed an increase of the major anti-oxidative redox buffer glutathione under high sulphur nutrition in response to fungal colonization. Our study demonstrates the existence of sulphur nutrition-enhanced resistance of tomato against Verticillium dahliae mediated by sulphur-containing defence compounds.  相似文献   
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