Analysis of the Fusarium mycotoxin moniliformin in cereal samples using 13C2-moniliformin and high-resolution mass spectrometry     

von Bargen KW  Lohrey L  Cramer B  Humpf HU 《Journal of agricultural and food chemistry》2012,60(14):3586-3591

Moniliformin is a mycotoxin produced by fungi of the Fusarium genus and occurs as a contaminant of different cereals worldwide. This study describes the first application of isotopically labeled (13)C(2)-moniliformin for the analysis of moniliformin in cereals. Moniliformin is a small and ionic molecule that forms only a single sensitive fragment ion in the collision cell of a tandem mass spectrometer. Therefore, the methods described in the literature for this kind of instrument observe only a single mass transition and show a relatively poor sensitivity. The use of high-resolution mass spectrometry was described to be a suitable alternative technique for the detection of this compound and was therefore applied in this study. The developed method is based on the use of strong anion exchange columns for cleanup prior to HPLC analysis and has a recovery rate of 75.3%, a limit of detection (LOD) of 0.7 μg/kg, and a limit of quantitation (LOQ) of 2.5 μg/kg. Twenty-three different cereal samples were analyzed for their moniliformin content. Twenty of them showed positive results with levels up to 126 ± 12.2 μg/kg.  相似文献   

Astronomy. Brown dwarfs--faint at heart, rich in chemistry     

Lodders K 《Science (New York, N.Y.)》2004,303(5656):323-324

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Southern Hemisphere water mass conversion linked with North Atlantic climate variability     

Pahnke K  Zahn R 《Science (New York, N.Y.)》2005,307(5716):1741-1746

Intermediate water variability at multicentennial scales is documented by 340,000-year-long isotope time series from bottom-dwelling foraminifers at a mid-depth core site in the southwest Pacific. Periods of sudden increases in intermediate water production are linked with transient Southern Hemisphere warm episodes, which implies direct control of climate warming on intermediate water conversion at high southern latitudes. Coincidence with episodes of climate cooling and minimum or halted deepwater convection in the North Atlantic provides striking evidence for interdependence of water mass conversion in both hemispheres, with implications for interhemispheric forcing of ocean thermohaline circulation and climate instability.  相似文献   

Hepatic lobe torsion in a horse          下载免费PDF全文

Kristin J. Bentz  Brandy A. Burgess  Katharina L. Lohmann    Farshid Shahriar 《The Canadian veterinary journal. La revue veterinaire canadienne》2009,50(3):283-286

A 4-year-old Belgian mare was presented with a 1-week history of fever, suspected of being caused by peritonitis. The mare died before the diagnostic procedures had been completed; postmortem examination revealed torsion of the left medial lobe of the liver, resulting in diffuse necrosis of liver tissue and severe peritoneal effusion.  相似文献   

A reply to “The 1.8 kb DNA fragment formerly confirmed as Mycoplasma suis (M. suis) specific was originated from the porcine genome”     

Ludwig E. Hoelzle  Dagmar Adelt  Katharina Hoelzle  Karl Heinritzi  Max M. Wittenbrink 《Veterinary microbiology》2009,138(1-2)

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RADIOGRAPHIC DIAGNOSIS OF LAMENESS IN AFRICAN ELEPHANTS (LOXODONTA AFRICANA)     

Katharina M.  Hittmair  DR.MED.VET.  Hanna D.  Vielgrader  DR.MED.VET. 《Veterinary radiology & ultrasound》2000,41(6):511-515

Lameness in captive elephants is most commonly caused by pododermatitis or degenerative joint disease. Hard surfaces such as concrete, which produce a damp and cold environment, wet and muddy conditions, as well as restricted movement are the major causes of these problems. Radiography was performed in two African elephants at the Schoenbrunn Zoo in Vienna to determine the cause and extent of lameness. Various radiographic techniques are described for use in trained elephants. Low time settings were used to avoid loss of detail through movement and to minimize exposure while observing radiation safety. A 37-year-old elephant had front limb lameness due to an interdigital abscess. In radiographs of the foot an inhomogenous soft-tissue swelling without involvement of the phalanges was seen. Ultrasonography was helpful in visualizing the fluid-filled abscess. In additional joint radiographs severe degenerative joint disease was identified. A 13-year-old elephant had lameness of the hind limb. Radiographs of the hind limb from the foot to the stifle were made. Open physes and early signs of degenerative joint disease were identified on the radiographs.  相似文献