Effect of suckling on the reproductive performance and metabolic status of obese Japanese black cattle during the early postpartum period     

Kawashima C  Kida K  Matsuhashi M  Matsui M  Shimizu T  Matsunaga N  Ishii M  Miyake Y  Miyamoto A 《The Journal of reproduction and development》2008,54(1):46-51

The aim of our study was to investigate the effects of suckling on reproductive performance and metabolic status of obese (mean body condition score of more than 4.0 on a scale of 1-5) maternal Japanese Black cows during early postpartum period. We used 7 postpartum Japanese Black cattle. Four cows were suckled ad libitum (suckled) until completion of their first artificial insemination (AI), while 3 cows were not suckled at all because they were separated from their calves immediately after parturition (non-suckled). Body weight and plasma concentrations of metabolites and hormones were measured from wk 1 to 9 postpartum. Ovarian activity was detected using plasma progesterone concentration, and all cows received their first AI after application of the Ovsynch protocol at approximately 4 months postpartum. Although body weights of non-suckled cows increased during experimental period (P<0.05), those of suckled cows remained unchanged. Plasma concentrations of glucose of non-suckled cows were higher at wk 2 postpartum (P<0.05) and their levels of non-esterified fatty acid tended to be lower at wk 1 and 2 postpartum compared with suckled cows (P<0.1); however, these differences between groups were not observed with progression of postpartum period. In addition, plasma insulin concentrations of non-suckled cows were higher than those of suckled cows during experimental period (P<0.05). During sampling period (wk 0 to 9 postpartum), onset of normal ovarian cycle was observed in all non-suckled and 2 of 4 suckled cows, and it was delayed in other 2 suckled cows compared with non-suckled cows; however, 3 suckled cows conceived at the first AI after application of the Ovsynch protocol; none of non-suckled cows conceived at this time. Overall, we suggest that suckling seems to reduce increase of body weight after parturition, although it does not improve obesity, and influences conception despite delay in resumption of normal ovarian cyclicity in obese Japanese Black cows.  相似文献   

Conversion of a male-specific RAPD marker into an STS marker in Asparagus officinalis L.     

Akira Kanno  Shosei Kubota  Katsuya Ishino 《Euphytica》2014,197(1):39-46

Garden asparagus (Asparagus officinalis L.) is an economically important plant. This species is dioecious, and male plants are considered to be more desirable than females due to their higher yields. To reduce the time required for asparagus breeding, molecular marker techniques have been employed to identify sex-linked DNA markers. In the present study, we converted the male-specific random amplified polymorphic DNA marker T35R54-1600 into a sequence tagged site marker. We cloned a male-specific DNA fragment amplified with the T35R54 primer and determined the sequence of the fragment. The size of T35R54-1600 is 1,586 bp, and this fragment is not homologous to known sex-linked BAC sequences, indicating that this fragment is a new sex-linked region. Within this fragment, we designed the primer pair ‘MSSTS710’ to amplify a 710 bp region. This marker could be used to identify the sex of eight cultivars of A. officinalis: ‘Mary Washington 500W’, ‘UC157’, ‘Harumachi Green’, ‘Super Welcome’, ‘F4’, ‘Pacific 2000’, ‘F2’ and ‘Backlim’. We also analyzed the applicability of this marker to two dioecious Asparagus species, A. schoberioides and A. kiusianus, which are cross-compatible with A. officinalis. Although male-specific DNA fragments of two dioecious Asparagus species, A. schoberioides and A. kiusianus, were generated using the existing male-specific marker Asp1T7sp, no amplicon was obtained using the MSSTS710 marker. Since MSSTS710 can be employed for sex identification only in A. officinalis and not in closely related Asparagus species, the DNA region around the MSSTS710 marker must be variable among Asparagus species.  相似文献   

A convenient and nondestructive method using bio-impedance analysis to determine fish freshness during ice storage     

Pengxiang Yuan  Yao Wang  Riho Miyazaki  Jia Liang  Katsuya Hirasaka  Katsuyasu Tachibana  Shigeto Taniyama 《Fisheries Science》2018,84(6):1099-1108

Fish freshness can be assessed through K values, but this method has a number of limitations, including a complex procedure and destructive sampling. With the aim to develop a convenient method of assessing fish freshness, we measured the changes in K values (up to 40%) and bio-impedance (Z value; frequency 2, 5, 20, 50, 100 kHz) of ordinary muscle in fish of eight marine species, all caught in the East China Sea, during ice storage and examined their relationships. The results indicated that the K value in all fishes increased linearly with storage time, while their Z value decreased only after 24 h of storage. Moreover, after 24 h of storage and at K values of < 40%, impedance ratios at 2–100 kHz (C value, C?=?Z_{2 kHz}/Z_{100 kHz}) were significantly correlated (p ?<?0.05) with both storage time and K values in all fishes. These findings suggest that the bio-impedance ratio effectively reflects the change in ATP-related compounds of fish and that a convenient, nondestructive method using the C value can be used instead of the complicated K value measurement to assess the freshness of marine fishes after 24 h of ice storage.  相似文献   

Influence of after-ripening environments on the germination characteristics and seed fate of Italian ryegrass (Lolium multiflorum)     

MINORU ICHIHARA  MASAYUKI YAMASHITA  HITOSHI SAWADA  YOICHI KIDA  MOTOAKI ASAI 《Weed Biology and Management》2009,9(3):217-224

Italian ryegrass ( Lolium multiflorum Lam.) is an exotic winter annual weed that recently has seriously infested wheat fields in central Japan. To understand the influence of tillage systems on the germination ecology and seedbank dynamics of naturalized Italian ryegrass, we compared the fate of the seeds that were after-ripened in the soil with that of the seeds on the soil surface, and then examined the germination characteristics of the seeds retrieved from both treatments by germination tests in both a light/dark (12 h photoperiod) condition and in constant darkness. The seedling emergence was greater initially for the seeds after-ripened on the soil surface than for the seeds in the soil. The component of emergent seeds after-ripened on the soil surface increased as time passed during the summer, whereas that of emergent seeds in the soil scarcely increased. This contrasting emergence was, in part, attributable to the ability of the seeds to germinate at higher temperatures in the light/dark condition than in constant darkness. These results suggest that untilled systems, which leave many seeds on the soil surface, might promote the rapid depletion of Italian ryegrass seeds through germination before wheat sowing in early winter. In contrast, seed burial by tillage might inhibit the germination of the seeds and form a large transient seed bank available to germinate during the wheat-growing season.  相似文献   

An etiological investigation of domestic cats with conjunctivitis and upper respiratory tract disease in Japan     

Cai Y  Fukushi H  Koyasu S  Kuroda E  Yamaguchi T  Hirai K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(3):215-219

Chlamydophila felis (C. felis), feline herpesvirus-1 (FHV-1) and feline calicivirus (FCV) were detected in 39 (59.1%), 11 (16.7%) and 14 (21.2%) cats respectively, from 66 domestic cats presented with conjunctivitis and upper respiratory tract disease (URTD) in 9 prefectures of Japan. Dual and multiple infections were found in 7 (10.6%) cats with both C. felis and FHV-1, 10 (15.2%) cats with both C. felis and FCV, and 1 (1.5%) cat with all three agents. C. felis was isolated from 11 (28.2%) of 39 PCR positive cats. Antigenic difference was found in a 96 kDa protein of our isolates and Fe/145 strain isolated in USA. In conclusion, C. felis is the most common agent of feline conjunctivitis and URTD, and the coinfection with C. felis, FHV-1 and FCV are also common in cats in Japan.  相似文献   

Incidence of Salmonella infection in healthy dogs in Gifu Prefecture,Japan     

Fukata T  Naito F  Yoshida N  Yamaguchi T  Mizumura Y  Hirai K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1079-1080

A total of 1,013 feces samples and 8 mesenteric lymphonodus samples obtained from apparently healthy dogs were examined for the incidence of salmonella infection. One strain of S. typhimurium (ST) was isolated from feces of one dog, and S. enteritidis (SE) was isolated from the mesenteric lymphonodus of one dog. Sera obtained from 330 apparently healthy dogs were examined for Salmonella antibodies using an ELISA with heated whole cells of SE and ST. Fifty-one of the 330 serum samples were considered to be positive for salmonella antibodies, including 12 which were SE-positive and 39 which were ST-positive. These results indicate that dogs cause possible environmental problems as Salmonella carriers.  相似文献   

The metabolic profile test: its practicability in assessing feeding management and periparturient diseases in high yielding commercial dairy herds     

Kida K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(7):557-563

The aim of this study was to evaluate the practicability of using the metabolic profile test (MPT) as a preventive tool for periparturient disease of dairy cows. The MPT was assessed in 79 dairy herds with high incidence of periparturient disease and 76 healthy herds of cows producing more than 8,500 kg 305-day milk yield. The changes in metabolic profiles were also assessed in 17 dairy herds at two times, the first at high incidence of periparturient disease and the second after reduced incidence and improved feeding management. In the herds with high incidence of periparturient disease, low blood values of hematocrit, albumin, glucose, cholesterol, calcium and magnesium were observed in the dry period. These values correctly diagnosed malnutrition as the cause of periparturient diseases. Following feeding management changes, there was a low incidence of periparturient disease and the metabolic profiles were normal showing that feeding management had improved. We concluded that the MPT is a useful tool for assessing feeding management and periparturient diseases of dairy cows.  相似文献   

Characterization and distribution of Staphylococcus sp. implicated for improvement of fish sauce odor   总被引：1，自引：0，他引：1  

Katsuya  FUKAMI  Masataka  SATOMI  Yasuhiro  FUNATSU  Ken-Ichi  KAWASAKI  Shugo  WATABE 《Fisheries Science》2004,70(5):916-923

ABSTRACT:   The two Staphylococcus strains that had been isolated from fish sauce mush (moromi) made from frigate mackerel in Japan and proved to improve fish sauce odor, were examined for their taxonomic positions. The sequence analysis based on 16S rRNA and rpoB showed that the two strains, R4Nu and R5G, had an identical sequence with sequence identities of 99.5% and 99.0% to the above two genes from the closest species of S. nepalensis , respectively. A DNA hybridization test of the two strains showed more than 80% DNA similarity with S. nepalensis , thus confirming the above-mentioned species identification. Polymerase chain reaction primers specific to the strain isolated from fish sauce mush were designed from rpoB and examined for the distribution of this species to various fish sauces made in Asian countries as well as to fish sauce starter (malt) made from soy beans and barley in Toyama Prefecture, Japan. The amplified DNA fragment bearing the S. nepalensis gene was detected in the enriched culture of the malt, although no positive reaction was shown with fish sauce samples. These results suggest that S. nepalensis indebted to improve fish sauce odor was originated from the fish sauce starter malt.  相似文献   

Prevalence and Dynamics of Antibodies against NcSAG1 and NcGRA7 Antigens of Neospora caninum in Cattle during the Gestation Period     

Yasuhiro TAKASHIMA  Masaki TAKASU  Isao YANAGIMOTO  Naoki HATTORI  Tatiana BATANOVA  Yoshifumi NISHIKAWA  Katsuya KITOH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(11):1413-1418

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Foot rot disease of sweet potato in Japan caused by Diaporthe destruens: first report,pathogenicity and taxonomy     

Maeda  Ai  Minoshima  Ayaka  Kawano  Shinji  Nakamura  Misa  Takushi  Tetsuya  Yamashiro  Maki  Kawamura  Futoshi  Oshiro  Atsushi  Ichinose  Katsuya  Okada  Yoshihiro  Rossman  Amy Y.  Hirooka  Yuuri 《Journal of General Plant Pathology》2022,88(1):33-40

Journal of General Plant Pathology - Foot rot, also known as stem rot or tuber-root rot syndrome, on sweet potato occurs in sweet potato fields in Okinawa, Japan. Isolates of one fungal species...  相似文献