Identification and expression of a gene encoding an epitope that induces hemagglutination inhibition antibody to Avibacterium paragallinarum serovar A   总被引：1，自引：0，他引：1  

Noro T  Yaguchi K  Amimoto K  Oishi E 《Avian diseases》2007,51(1):84-89

The aims of this study were the identification, cloning, and expression of a genetic region encoding an epitope that induces hemagglutination inhibition (HI) antibody against Avibacterium paragallinarum serovar A and an evaluation of the recombinant protein for immunogenicity in chickens. Although two monoclonal antibodies (MAbs) with HI activity, designated S24-951 and S7-1716-5C, were generated in this study, no reactive proteins with both MAbs were identified by Western blot analysis. A gene fragment of 5157 bp, designated hpa5. 1, was cloned from genomic DNA, and a recombinant protein expressed by hpa5.1, designated HPA5.1, reacted with both MAbs on dot-blot analysis. HPA5.1 showed no hemagglutinating activity, but significantly absorbed HI antibodies in the chicken immune serum. Analysis using a series of deletion mutants prepared from hpa5.1 indicated that a 4.8 kbp gene in hpa5.1 is essential for the expression epitope recognized by MAb S24-951. In addition, chickens immunized once with HPA5.1 showed a high protection rate with sufficient HI antibody titers against challenge exposure with a virulent strain of A. paragallinarum serovar A strain 221. These results show that hpa5. I1 is responsible for the expression of an epitope that induces HI antibody, and HPA5.1 might be a candidate for the development of a new vaccine against avian infectious coryza caused by A. paragallinarum serovar A.  相似文献   

Evaluation of total mixed ration silage with brewers grains for dairy buffalo in Tarai,Nepal          下载免费PDF全文

Takashi Sakai  Naba R. Devkota  Kazato Oishi  Hiroyuki Hirooka  Hajime Kumagai 《Animal Science Journal》2015,86(10):884-890

To investigate the effects of total mixed ration (TMR) silage, which contained brewers grain and rice straw as a substitute for conventional concentrate on feed intake and milk production in middle‐to‐late lactation buffaloes, four multiparous Murrah buffaloes were assigned to a 3 × 3 Latin square design experiment. The TMR silage, which had higher neutral and acid detergent fiber contents and digestibility than concentrate (P < 0.05) and similar crude protein (CP) and total digestible nutrient (TDN) contents with concentrate were used for the lactation experiment. The treatments were control (CTL) fed concentrate at 0.6% of body weight (BW), and T1 and T2 fed the TMR silage at 0.6 and 1.2% of BW on a dry matter (DM) basis, respectively, with rice straw ad libitum. Daily intakes of DM, CP and TDN, and BW change were higher in T2 than in CTL and T1 (P < 0.05). Although milk composition did not differ among the treatments, milk yield (MY) was higher in T2 (P < 0.05). There were no significant differences in MY/DM intake and MY/TDN intake among the treatments. The increase of BW and MY in middle‐to‐late lactation buffaloes might have been due to high TDN intake from supplementary TMR silage.  相似文献   

Effect of 50 kilobase-plasmid, pKDSC50, of Salmonella choleraesuis RF-1 strain on pig septicemia.     

H Danbara  R Moriguchi  S Suzuki  Y Tamura  M Kijima  K Oishi  H Matsui  A Abe  M Nakamura 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(6):1175-1178

Salmonella choleraesuis strains with and without 50-kilobase plasmid (pKDSC50) were intravenously inoculated into Yorkshire pigs. By the inoculation of 7.2 x 10(5) - 3.5 x 10(7) cells, RF-1 strain with pKDSC50, but not 31N-1 strain without the plasmid, caused a septicemia. The inoculation of 8.7 x 10(9) RF-1 cells killed pigs at 2-4 day postinfection with severe hemorrhage on the whole body. Pigs with a similar number of 31N-1 cells (8.3 x 10(9) cells), showed milder hemorrhage, and they died at 6 day postinfection. These results indicated that pKDSC50 is required for RF-1 strain to express the full virulence causing a heavy cutaneous pig septicemia.  相似文献   

EPIZOOTIOLOGIC STUDY OF MID-CYCLE DISEASE OF LARVAL Macrobrachium rosenbergii     

G. Akita  R. Nakamura  G. Miyamoto  J. Brock  M. Fujimoto  F. Oishi  D. Onizuka  D. Sumikawa 《Journal of the World Aquaculture Society》1981,12(2):223-230

Mid-cycle disease (MCD) of larval Macrobrachium rosenbergii has reduced survival of larval cultures at the state facility from a normal 50–70% of the population at the post-larval stage, to 5–10%. Observations revealed a characteristic mortality pattern, where a peak in daily mortality occurred near the middle of the rearing cycle. Epizootiologic studies were initiated using existing facilities, to aid in characterization of MCD and elucidate possible causes. Experiments were undertaken in duplicate culture tanks, each separated into 4 equal volume compartments by nitex screen. Water, algae, and some feed could pass between compartments; prawn larvae could not. “Reference” larvae were introduced into a compartment of each tank on the day of hatch. “Test” larvae populations of different ages and sources were introduced into the remaining compartments at various times after the initiation of the experiment. Dead larvae in the bottom sediment were counted daily and mortality curves generated. Length and developmental stage of live and dead animals in each compartment were assessed daily. Results indicated that a “toxic period” of unknown etiology began on the 10th day of culture and probably extended for the duration of the rearing cycle. “Test” populations introduced during the “toxic period” displayed a mortality peak within 4–6 days. Larvae at age 15 days (stage 6–7) or older appeared refractory to the “toxicity.” MCD apparently reduced growth rates (length) but did not appear to selectively kill larger, faster growing, susceptible individuals. Problematically large portions of animals lost from populations were not accounted for. Studies on MCD will continue.  相似文献   

Responses of fees used for revegetation of desert areas to iron deficiency stress     

Yoshikazu Kiriiwa  Hiromi Yokota  Atsushi Oishi 《Soil Science and Plant Nutrition》2013,59(4):563-568

We investigated the responses to iron deficiency of four major tree species, Prosopis cineraria (local name: Ghaf), Acacia tortilis (Samar), Zizyphus spina-christi (Sidr), and Leptadenia pyrotechnica (Markh), used for revegetation of desert areas in the United Arab Emirates (UAE). The responses to iron deficiency differed among the tree species. Ghaf, Samar, and Sidr decreased the nutrient solution pH in response to iron deficiency. Markh and iron-deficient Sidr did not release protons in the CaCl₂ solution. The Fe reducing capacity of the roots of Ghaf, Samar, and Sidr was significantly enhanced by iron deficiency, whereas a smaller increase in the reducing activity of the roots of Markh was observed. In all the tree species, the amount of reductant released from iron-deficient plants was higher than that from iron-sufficient ones. Markh released a small amount of reductant under irondeficient conditions. In the present study, the reductant released by all the trees was caffeic acid. Acidification treatment enhanced the amount of reductant released irrespective of iron treatments in Ghaf, Samar, and Sidr but had no effect on the amount of reductant released in iron-deficient Markh. The activity of p-coumarate hydroxylase was higher in Sidr and Ghaf than in the other trees. This activity decreased in iron-deficient Samar. Ghaf, Samar, and Sidr showed a high capacity to lower the pH of the nutrient solution, and Ghaf markedly enhanced the effectiveness of all the examined mechanisms, while Markh showed a lower ability compared to the other tree species.  相似文献   

Pneumonia in horses induced by intrapulmonary inoculation of Streptococcus equi subsp. zooepidemicus     

Yoshikawa H  Yasu T  Ueki H  Oyamada T  Oishi H  Anzai T  Oikawa M  Yoshikawa T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(7):787-792

To evaluate the possibility that Streptococcus equi subsp. zooepidemicus (S.z) the causative bacterial agent of equine shipping fever pneumonia (ESFP), as well as to investigate its pathogenesis, 10 horses (seven Thoroughbreds and three Anglo-Arab species, ranging from 2-4 years in age) were experimentally inoculated, via an endoscope, into bronchus of the lung lobe with a dose of 30 ml of 1-7 x 10(8) CFU/ml of S.z. After inoculation, autopsy and pathological examinations were sequentially conducted 30 min, 1, 2, 3, 4, 17, 20 hr and 2 weeks later. Pneumonia induced by the intrapulmonary inoculation of S.z was characterized by small purulent pneumonic foci in the inoculated areas. With the lapse of time, these foci developed into serous hemorrhagic pneumonia, hemorrhagic purulent pneumonia, and then purulent, coagulation necrotic pneumonia. These pathomorphological characteristics of experimental pneumonia closely resemble those naturally occurring ESFP. There is strong evidence that S.z. is implicated as a causal factor in ESFP. S.z. grew in the mucus, exudate, and pulmonary effusions. Further, the bacteria showed resistance against phagocytosis by pulmonary alveolar macrophages (PAM) and neutrophils. Inhibition of PAM and neutrophil function is considered to be important in the development of pneumonia. With the progression of the disease, the neutrophils often adhered to the endothelial surface of the alveolar capillary lumen and played a role in generating coagulation necrosis of lung tissues.  相似文献   

Microsatellite polymorphism in Japanese mongrel dogs     

Oishi N  Maeda M  Makimura K  Sawaguchi T  Hayashiya M  Kubo T  Kano R  Hasegawa A  Kasahara M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(10):1055-1057

The genetic variability of 182 unrelated mongrel dogs living in various areas of Japan (from Hokkaido to Okinawa) was studied by collecting their blood. Ten microsatellite loci were chosen from different autosomal chromosomes. After combining a few rare adjoining alleles to allelic classes, it was confirmed that the Hardy-Weinberg equilibrium was attained in each locus. The polymorphic information contents (PICs) of the loci, Ren37A11, Ren48E01, AHTk253, ZuBeCa30, Ren277K09, Ren42N13, AHT130, PEZ03, PEZ12, and AHT121, were 0.58, 0.63, 0.67, 0.67, 0.68, 0.71, 0.79, 0.80, 0.80, and 0.80, and the power of discriminations (PDs) were 0.80, 0.85, 0.87, 0.88, 0.88, 0.89, 0.94, 0.94, 0.94, and 0.94, respectively. The combined mean exclusion chance (MEC) was 0.9995, indicating that these microsatellite loci are useful for kinship testing of Japanese dogs.  相似文献   

Selection experiment for increased egg production under 23 H and 24 H light-dark cycles in the domestic fowl     

M Naito  K Nirasawa  T Oishi  T Komiyama 《British poultry science》1989,30(1):49-60

A selection experiment was conducted for increased rate of lay under 23-h (23 HS line) and 24-h (24 HS line) light-dark cycles over 5 generations. In generation 5, rate of lay was higher in the 23 HS line than in the 24 HS line, and the proportion of hens with mean intra-sequence intervals of less than 24 h in the 23 HS line increased to 88% in the generation 5 from 15% in the base generation. The realised heritability for rate of lay was 0.25 plus/minus 0.04 in the 23 HS line and 0.15 plus/minus 0.05 in the 24 HS line. Egg weight and shell weight decreased in both lines, and the decrease in the proportion of shell was larger in the 23 HS line than in the 24 line. It is suggested that a regimen of 23 h light and dark may be an effective environment for selection to improve the laying performance of a population approaching a plateau for egg production.  相似文献   

Induction of dog sperm capacitation by glycosaminoglycans and glycosaminoglycan amounts of oviductal and uterine fluids in bitches     

Kawakami E  Arai T  Oishi I  Hori T  Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(1):65-68

Ejaculated sperm collected from 12 beagle dogs were incubated in canine capacitation medium (CCM), supplemented with 5 microg/ml chondroitin sulfate A (CS), 5 microg/ml hyaluronic acid (HA), or 5 microg/ml heparin (HP) for 7 hr at 38 degrees C in a 5% CO2 in air atmosphere to investigate the effects of glycosaminoglycans (GAGs) on dog sperm capacitation. The percentages of motile sperm, hyperactivated sperm (%HY), and acrosome-reacted sperm (%AR) in all media were examined after 4 hr and 7 hr of incubation. The oviducts and uteri of 9 anestrous and 18 estrous beagle bitches were removed under halothane inhalation anesthesia to measure the total GAG amounts in oviductal and uterine fluids. The lumens of the ampulla of the oviducts, isthmus of the oviducts, and the uterine horns were each flushed with 1 ml HEPES-EDTA fluid. Total GAG amounts in the flush fluids obtained were measured with a spectrophotometer. Sperm motility (51-59%), %HY (79-86%), and %AR (31-36%) in CCM supplemented with CS, HA, or HP were significantly higher after 7 hr of incubation than when incubated in CCM without GAGs (P<0.01 or 0.05). The mean total GAG amounts in the fluids from the ampulla and isthmus of the oviducts and the uterine horns in the estrous bitches were higher than in the anestrous bitches. These results indicate that GAGs in the oviductal and uterine fluids in estrous bitches are associated with in vivo sperm capacitation.  相似文献   

Effect of parental genotypes and paternal heterosis on litter traits in crossbred goats.     

Y Tsukahara  Y Choumei  K Oishi  H Kumagai  A K Kahi  J M Panandam  T K Mukherjee  H Hirooka 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2008,125(2):84-88

The effect of parental genotype and paternal heterosis on litter size (LS), total litter birth weight (TLW) and average litter birth weight (ALW) was analysed utilizing data from a crossbreeding programme involving the exotic German Fawn goats and local Katjang goats in Malaysia. In this study, these traits were regarded as traits of the litter to consider the effect of service sire genotype. The results revealed that LS was significantly influenced by the genotype of sire. The genotypes of sire and dam had significant effects on TLW and ALW. Estimates of crossbreeding parameter showed significant and negative influence of paternal heterosis on TLW and ALW while there was no significant effect of paternal heterosis on LS. The results of this study stress the need to reconsider the use of local males in the tropics.  相似文献