Immunohistochemical detection of P-glycoprotein (PGP) and multidrug resistance-associated protein (MRP) in canine cutaneous mast cell tumors     

Miyoshi N  Tojo E  Oishi A  Fujiki M  Misumi K  Sakamoto H  Kameyama K  Shimizu T  Yasuda N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):531-533

Fifty-four canine cutaneous mast cell tumors were evaluated immunohistochemically for the expression of P-glycoprotein (PGP) and multidrug-resistance-associated protein (MRP). All tumors examined were graded according to the histological malignancy. ranging from grade I to III. The expression of PGP was confirmed in 15% (8/54) of whole, 33% (5/15) of grade I, 10% (3/31) of grade II, and 0% (0/8) of grade III tumors. The expression of MRP was found in 18% (10/54) of whole, 26% (4/15) of grade I, 19% (6/31) of grade II, and 0% (0/8) of grade III tumors. The cases positive to at least one of these 2 multidrug markers were 26%, 47%, 23% and 0% of whole and grade I to III tumors, respectively. These results indicate that at least 26% of canine cutaneous mast cell tumors express PGP and/or MRP and that these tumors may be resistant to several anti-cancer drugs.  相似文献   

Evaluation of plasma erythropoietin levels in normal adult dogs by in vivo bioassay using concentrated plasma.     

A Oishi  H Sakamoto  R Shimizu  F Ohashi  A Takeuchi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(4):621-628

Measurement of plasma erythropoietin level in normal dogs by in vivo bioassay has been considered to be impossible so far. In the present study, we successfully determined it by using concentrated plasma 60 times which allowed the lower limit to 2.7 mU/ml. This normal plasma erythropoietin level was the first to be determined as an in vivo bioactivity and was 9.14 +/- 7.81 mU/ml in 75 normal adult dogs. This value was sufficiently reliable in terms of accuracy of determination and considered to be meaningful as the low level in vivo bioactivity that hasn't been known to date. Furthermore, erythropoietin levels in normal plasma were within a certain lower range and showed neither difference in plasma erythropoietin level between males and females or among breeds nor correlation between erythropoietin and hemoglobin level.  相似文献   

Production of quail-chick chimaeras by blastoderm cell transfer   总被引：2，自引：0，他引：2  

M Naito  M Watanabe  M Kinutani  K Nirasawa  T Oishi 《British poultry science》1991,32(1):79-86

1. Quail-chick chimaeras were produced by injecting dissociated quail blastoderm cells into chick embryos. 2. Quail blastoderms were removed from the yolk and the cells were dispersed by trypsin treatment or pipetting. The cell suspension (1 to 5 microliters) was injected into the subgerminal cavity of unincubated chick embryos. The chick embryos were then cultured in recipient eggshells. 3. Quail blastoderm cells injected into the chick embryos adhered to the chick embryonic cells. The rates of hatching were 8.6% (38 chicks from 441 eggs) and 40.3% (48 chicks from 119 eggs) when the volumes of the cell suspension injected were 3 to 5 microliters and 1 microliter, respectively. 4. Seven out of 86 hatched birds were clearly identified as being chimaeric because part of the feather colouring was of quail specificity. In addition to these chimaeric birds, there were 8 chimaeric embryos which died before hatching. The distribution patterns of the quail feathers were varied among the chimaeric birds and embryos. 5. This technique provides a basis for the investigation of chick embryo cryopreservation, genetic transformation and analysis of cell lineage of chickens.  相似文献   

Effects of supplementary desalted mother liquor as replacement of commercial salt in diet for Thai native cattle on digestibility,energy and nitrogen balance,and rumen conditions          下载免费PDF全文

Yoshiaki Sato  Wanna Angthong  Patima Butcha  Motoharu Takeda  Kazato Oishi  Hiroyuki Hirooka  Hajime Kumagai 《Animal Science Journal》2018,89(8):1093-1101

Four Thai native cattle were used in a 4 × 4 Latin square design experiment to evaluate the availability of desalted mother liquor (DML) as replacement of salt in concentrate. Each cattle was assigned to one of the following concentrate feeding treatments: C1, 1% NaCl was added as salt; C2, 2% NaCl was added as salt; D1, 1% NaCl was replaced by DML; D2, 2% NaCl was replaced by DML, on a dry matter (DM) basis. The animals were fed rice straw and experimental concentrates (40:60) at 1.9% of body weight on a DM basis, daily. Acid detergent fiber expressed exclusive of residual ash (ADFom) digestibility in DML treatment was higher than salt treatment (p < .05) and D2 feeding showed the highest value (60.8%). There were no significant differences in blood metabolites, nitrogen retention, ruminal ammonia nitrogen, methane emission or energy efficiency among treatments. Molar percent of acetate on volatile fatty acids in rumen fluid 4 hr post‐feeding tended to be higher in DML treatment than salt treatment (p = .08). The results indicated that adding DML could improve ADFom digestibility and salt could be replaced by DML up to 2% as NaCl in concentrate without adverse effects on nitrogen balance, rumen conditions, blood metabolites and methane emission.  相似文献   

French consumers’ marginal willingness to pay for the pairing of Japan’s fall chum salmon and rice wine (sake)     

Oishi  Taro  Sugino  Hiroaki  Yagi  Nobuyuki 《Fisheries Science》2022,88(6):845-856

The pairing of foods and beverages (especially alcoholic beverages) is an effective marketing approach to promote the value of Japanese gastronomy to overseas consumers because it requires little additional cost. In this study, the research target was French consumers, and the evaluation targets were grilled fall-season wild chum salmon from Japan (mainly from Hokkaido) and Japanese rice wine (sake). The difference in the consumers’ willingness to pay (WTP) was evaluated using the contingent valuation method. In addition, we analyzed the determinants by applying Tobit regression. Our results revealed that there was a higher WTP when Japan’s (Hokkaido’s) grilled fall salmon and sake were presented as a set than when they were presented individually. The geographical identity of the origin of food and beverages can engender a positive premium to consumers through the synergistic effect of the origin of the brand.

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In vitro ruminal fermentation and in situ ruminal degradation of tamarind kernel powder extract residue in wethers          下载免费PDF全文

Lin Wang  Kazato Oishi  Yoshiaki Sato  Takashi Nakanishi  Hiroyuki Hirooka  Kei Takahashi  Hajime Kumagai 《Animal Science Journal》2017,88(7):966-973

We examined the in vitro fermentation characteristics and in situ dry matter (DM) and crude protein (CP) degradability of tamarind kernel powder extract residue (TKPER), a by‐product of polysaccharides thickener processing. Two types of TKPER (I and II), of which the CP and neutral detergent fiber organic matter basis contents (%) were 41.4 and 42.0 and 1.4 and 0.5, respectively, were compared with dry heat soybean (SB), soybean meal (SBM) and dry soybean curd residue (SBCR). The TKPERs had significantly lower in vitro gas production compared to the other products at each observation time (P < 0.05). The in vitro DM and CP digestibility (%) of TKPER I and II were 67.7 and 64.9, and 64.5 and 58.0, respectively, significantly lower than those of SB and SBM (P < 0.05). We used four wethers (55.6 ± 4.6 kg) with ruminal cannulas to investigate the in situ degradability of TKPER I, SB, SBM and SBCR. At the ruminal outflow rate of 0.05/h, the effective degradability (%) values of the DM and CP of TKPER I were 64.1 and 49.7, respectively, which were similar to those of SBM. In conclusion, TKPER had high CP and exhibited in situ degradability similar to that of SBM, suggesting that TKPER could be used as a protein source feed.  相似文献   

Health and mineral nutrition status of yaks in southern Mustang,Nepal          下载免费PDF全文

Hajime Kumagai  Mitsumi Nakajima  Hiroki Anzai  Takashi Sakai  Kazato Oishi  Hiroyuki Hirooka  Manoj Kumar Shah 《Animal Science Journal》2017,88(8):1156-1161

Biochemical values and mineral concentrations in blood plasma were investigated to evaluate the statuses of health and mineral nutrition among yaks in Mustang District, Nepal. In total, 118 plasma samples of female yaks collected in April and September/October of 2013–2015 were offered. Seventy‐four percent of yaks showed lower plasma total‐cholesterol concentrations than the lowest limit of reference range (100 mg/dL) and the values in spring (83.41 mg/dL) were lower (P < 0.05) than those in autumn (95.05 mg/dL). All the yaks had lower plasma albumin concentrations than the lowest limit of reference range (3.0 g/dL) and 66% of yaks showed lower plasma inorganic phosphorus concentrations than the critical level of phosphorus deficiency (4.5 mg/dL). Thirty‐five percent of yaks showed lower plasma calcium concentrations than the lowest limit of normal range (8 mg/dL) and the concentrations were lower in spring than in autumn (P < 0.01). Seventy‐five percent of yaks presented lower copper concentrations than the critical level (0.65 mg/L) and the concentrations were lower in spring than in autumn (P < 0.01). Since the low plasma total‐cholesterol might have indicated shortage of dry matter and energy intake, attention should be paid to the nutritional statuses of energy, phosphorus, calcium and copper in winter and early spring.  相似文献