Rumen fermentation,milk production and conjugated linoleic acid in the milk of cows fed high fiber diets added with dried distillers grains with solubles     

Hiroko SHIBATA  Shoko TATENO  Shuhei KANDA  Kazuki TAKAURA  Souichi ISHIDA  Hisao ITABASHI 《Animal Science Journal》2013,84(2):106-112

The effects of corn dried distillers grains with solubles (DDGS) feeding on rumen fermentation and milk production in cows were evaluated using diets high in neutral detergent fiber (NDF, 45.9–46.6%). The control diet (Control) consisted mainly of hay, corn silage and concentrates. In the experimental diets, the concentrates were replaced with DDGS as 10% dry matter (DM) (10%DDGS) and 20% DM (20%DDGS). Eight cows were used for each 14‐day treatment period. Effect of DDGS feeding on DM intake was not significant. Ruminal volatile fatty acids and ammonia‐N at 5 h after feeding of 20%DDGS were decreased compared to Control, whereas protozoal count at 2 h after feeding of 20%DDGS was higher than that of 10%DDGS. Milk yield of cows fed DDGS diets was greater than that of Control, although percentages of milk protein and solids‐not‐fat were decreased by DDGS diets. The proportions of C10:0, C12:0, C14:0 and C16:0 in the milk fat decreased, and those of C18:0, C18:1, C18:2 and cis‐9, trans‐11 conjugated linoleic acid (CLA) increased markedly with elevated DDGS. Increase in trans‐11 C18:1 was observed in the rumen fluid at 5 h after feeding. These findings suggest that DDGS feeding enhanced milk yield, as well as CLA synthesis under a high dietary NDF condition.  相似文献   

Methicillin‐resistant Staphylococcus aureus keratitis in a dog     

Kazuki Tajima  Akiko Sinjyo  Toshio Ito  Yoshizumi Noda  Hiroshi Goto  Norihiko Ito 《Veterinary ophthalmology》2013,16(3):240-243

The purpose of this study is to report a case of methicillin‐resistant Staphylococcus aureus (MRSA) keratitis in a dog. A 7‐year‐old intact male American cocker spaniel that had undergone removal of a nictitating gland was referred for severe ulcerative keratitis. Slit‐lamp examination showed swelling of the eyelid, mucopurulent discharge, conjunctival injection and chemosis, diffuse corneal edema and opacity, and a deep ulcer in central cornea. Gram staining of discharge from the eye demonstrated Gram‐positive cocci. Despite topical ofloxacin, oxytetracycline and polymyxin B ophthalmic solution and intravenous cefazolin, there was no improvement. Cultures revealed MRSA that was sensitive only to chloramphenicol, vancomycin, lincomycin, and clindamycin. The antibiotic regimen was changed to topical and systemic chloramphenicol. After 9 days of treatment, although inflammation started to be resolved, the dog developed nonregenerative anemia. The antimicrobial regimen was changed again to topical and systemic vancomycin. Inflammation continued to improve over the next week. MRSA should be considered a potential organism in infectious keratitis, especially when general antibiotics are not effective. Although topical and systemic chloramphenicol and/or vancomycin are effective for treating MRSA keratitis, vancomycin should only be used when culture and susceptibility results indicate it is appropriate and no other options are available. To our knowledge, this is the first detailed case report of MRSA keratitis in a dog.  相似文献   

Application of Enrofloxacin and Orbifloxacin Disks Approved in Japan for Susceptibility Testing of Representative Veterinary Respiratory Pathogens     

Kazuki HARADA  Masaru USUI  Tetsuo ASAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(10):1427-1430

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Synchronization of cellular clocks in the suprachiasmatic nucleus     

Yamaguchi S  Isejima H  Matsuo T  Okura R  Yagita K  Kobayashi M  Okamura H 《Science (New York, N.Y.)》2003,302(5649):1408-1412

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Novel reovirus isolation from an Ostrich (Struthio camelus) in Japan     

Sakai K  Ueno Y  Ueda S  Yada K  Fukushi S  Saijo M  Kurane I  Mutoh K  Yoshioka K  Nakamura M  Takehara K  Morikawa S  Mizutani T 《Veterinary microbiology》2009,134(3-4):227-232

An orthoreovirus was isolated from an Ostrich (Struthio camelus) and rapidly identified as orthoreovirus by the rapid determination of viral RNA sequences (RDV) system and electron microscopy. Phylogenetic analysis of the sigma A protein indicated that the isolate belonged to avian species and was closely related to chicken orthoreovirus strain 138. The results of the present study indicated that an ostrich orthoreovirus is slight different from other chicken orthoreoviruses and provided evidence of diversity among avian orthoreoviruses. To our knowledge, this is the first genetic report of an orthoreovirus isolated from an ostrich.  相似文献   

Changes in peroxisome proliferator-activated receptor gamma gene expression of chicken abdominal adipose tissue with different age, sex and genotype     

Kan SATO  Hiroyuki ABE  Tatsuyoshi KONO  Makoto YAMAZAKI  Kazuki NAKASHIMA  Toshihiko KAMADA  Yukio AKIBA 《Animal Science Journal》2009,80(3):322-327

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Enhanced local recruitment of glass eel <Emphasis Type="Italic">Anguilla japonica</Emphasis> in Oyodo River,Miyazaki and offshore environmental conditions in 2002     

Kazuhiro Aoki  Toshihiro Yamamoto  Nobuto Fukuda  Kazuki Yokouchi  Hiroaki Kurogi  Takashi Setou  Hiroshi Kuroda  Takahiko Kameda  Kazuhiro Takafuji  Tsutomu Tokeshi 《Fisheries Science》2018,84(5):777-785

To examine the environmental factors controlling the inshore recruitment dynamics of Anguilla japonica in the Oyodo River, Miyazaki Prefecture, Kyushu, glass eel samplings were carried out using fyke nets during winter (November–March) of 1994–2014. The peak CPUEs (catch per unit effort) were observed between November and February, but differed from year to year. The yearly CPUE was extremely high in 2002, when the sea surface temperature (SST) in the offshore area of the Oyodo River was the lowest in winter of all the sampling years. The negative SST anomaly of less than ? 0.5 °C was sustained in the offshore area during the recruitment season in 2002, which was caused by two combined factors; low air temperature and the Kuroshio path. The oceanographic data showed that the dominant path of the Kuroshio was displaced eastward at 31°N in 2002, which was different from the average Kuroshio path. The eastward displacement of the Kuroshio induced a cyclonic mesoscale eddy in the offshore area of the Oyodo River, resulting in the entrainment of the cold seawater into coastal waters from deep water. The oceanographic condition in relation to the continuous low SST could be favorable for local recruitment of glass eels.  相似文献   

Migration and spawning behavior of the greater amberjack Seriola dumerili in eastern Taiwan     

Kazuki Tone  Yosuke Nakamura  Wei-Chuan Chiang  Hsin-Ming Yeh  Sheng-Tai Hsiao  Chun-Huei Li  Kazuyoshi Komeyama  Masanori Tomisaki  Takamasa Hasegawa  Takashi Sakamoto  Itsumi Nakamura  Yoshitaka Sakakura  Ryo Kawabe 《Fisheries Oceanography》2022,31(1):1-18

Greater amberjack (Seriola dumerili) is an important fishery resource with a circumglobal distribution from tropical to temperate waters. Here, we investigated the spawning migration and habitat utilization of S. dumerili in the East China Sea (ECS). Archival tags were attached to 22 adult fish to examine their horizontal and vertical movements and estimate the spawning ground. S. dumerili were captured and released in the coastal waters of eastern Taiwan on November of 2016 and 2017. Information from seven pop-up satellite archival tags and seven depth–temperature recorders was recovered. Almost all of the fish stayed in the Taiwanese exclusive economic zone. Most individuals moved from released site to the southern edge of the ECS and showed behavior associated with the topographic features in winter (November to December). These phenomena may be related to foraging and be driven by oceanographic features such as the seasonal monsoon and the Kuroshio. The fish then migrated to the south offshore area of Taiwan in January and February. During their southward migration, the fish experienced a slowly elevated water temperature regime (SETR), which is one of the environmental factors that induce final oocyte maturation. In the spawning season (February to April), tagged females exhibited continuous diel vertical movements (DVMs) after experiencing the SETR. These continuous DVMs were observed over a wide geographic range from north to south in the Kuroshio off eastern Taiwan. Our study demonstrated that the putative spawning ground of S. dumerili must extend further in a north–south direction than predicted in a previous study.  相似文献   

Depletion of naturally recruited wild Japanese eels in Okayama,Japan, revealed by otolith stable isotope ratios and abundance indices     

Kenzo Kaifu  Kazuki Yokouchi  Tomihiko Higuchi  Hikaru Itakura 《Fisheries Science》2018,84(5):757-763

To investigate the population dynamics of naturally recruited wild Japanese eels, fisheries data of wild individuals in Okayama Prefecture were investigated as a case study. Wild and stocked eels were discriminated using a recently developed method based on otolith stable isotopes. Of the 161 eels captured in freshwater areas where eels had been stocked, 98.1% were discriminated as stocked. In contrast, 82.8% of 128 eels captured in coastal areas where eels are not stocked were discriminated as wild. There was a significant decrease in longline and set-net catch per unit effort between 2003 and 2016 in the coastal areas where most eels were discriminated as wild, indicating ongoing depletion of wild Japanese eels in these waters.  相似文献