Age related decreases in neural sensitivity to NaCl in SHR-SP     

Osada K  Komai M  Bryant BP  Suzuki H  Tsunoda K  Furukawa Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(3):313-317

To determine whether neurophysiological taste responses of young and old rats are different, recordings were made from the whole chorda tympani nerve which innervates taste buds on the anterior tongue. SHR-SP (Stroke-Prone Spontaneously Hypertensive Rats) in two age groups were studied. Chemical stimuli included single concentrations of 250 mM NH(4)Cl, 100 mM NaCl, 100 mM KCl, 500 mM sucrose, 20 mM quinine-hydrochloride, 10 mM HCl, 10 mM monosodium glutamate (MSG), 10 mM L- glutamic acid (L-Glu) and an NaCl concentration series. The magnitude of the neural response (response ratio) was calculated by dividing the amplitude of the integrated response by the amplitude of the spontaneous activity that preceded it. Substantial neural responses to all chemicals were obtained at both ages. The responses to KCl, sucrose, quinine-hydrochloride, HCl, monosodium glutamate (MSG) and glutamic acid (Glu) did not change with age, but the response to NaCl did decrease significantly. The profile of the response/concentration function for NaCl differed with age. In particular, the responses to solutions more concentrated than 100 mM NaCl were significantly weaker in aged than in young SHR-SPs. We also observed that recovery from amiloride treatment on the tongue of SHR-SPs was faster in aged rats than in young ones, suggesting that there is some functional difference in the sodium-specific channels on the taste cell. These results suggest that aged SHR-SP may be less able than young SHR-SPs to discriminate among higher concentrations of NaCl solutions.  相似文献   

Effects of supplement feeding order on lactation, diurnal variation of ruminal ammonia and urea in the blood and milk of dairy cows     

Kentarou IKUTA  Kazumi SASAKURA  Kazuhiro NISHIMORI  Careen HANKANGA  Keiji OKADA  Jun YASUDA 《Animal Science Journal》2005,76(1):29-36

The present study examined the effects of feeding order of grain and protein supplements on ruminal nitrogen (N) loss through the diurnal variation of ruminal ammonia N and urea N in the blood and milk of dairy cows. Three ruminally fistulated cows were used in a 3 × 3 Latin square arrangement of treatments; that is, grain supplement fed at the first feed and then the protein supplement at the second (GS‐1st), protein supplement fed first and then the grain supplement (PS‐1st), and the grain and protein supplements mixed and divided into equal portions fed at each supplement feeding time (GP‐mix). No differences in the lactation parameters were observed among the treatments. In GS‐1st treatment, the increase in the concentration of ruminal ammonia N was inhibited after feeding, and the concentration was lower (P < 0.05) than with the other treatments 1–2 h after basal feeding. The concentrations of urea N in the blood and milk with the GS‐1st treatment were the lowest of all treatments throughout the sampling times, and were lower (P < 0.05) than with the GP‐mix treatment except for blood levels 1 h after basal feeding. The results suggest that grain feeding before protein feeding can reduce ruminal N loss.  相似文献   

Quantitative estimation of the predation of marine ranched striped jack <Emphasis Type="Italic">Pseudocaranx dentex</Emphasis> by herons     

Harunao Nibe  Naoki Suzuki  Kazutaka Sakiyama  Shinsuke Morioka  Atsushi Ohno 《Fisheries Science》2010,76(3):473-480

The Goto station of the Japan Sea-Farming Association conducted marine ranching experiments with striped jack Pseudocaranx dentex from 1988 to 1999 in the Goto Islands, Nagasaki, Japan. Marine ranching is a stocking method where released fish are fed for some period after release in order to decrease initial mortality from starvation. Ranched fish were fed for 0, 90, and 15 days after stocking in 1997, 1998, and 1999, respectively. During the experiment, herons (grey heron Ardea cinerea, black-crowned night heron Nycticorax nycticorax, and great egret Egretta alba) were often observed preying on released fish in the ranching area. The present study estimated the number of fish preyed upon by herons during observation periods in 1997, 1998, and 1999 from the number of herons that flew into the ranching area and the predation rate on ranched fish by herons. The former was counted during sightings. The latter was estimated from the sighting observations and heron regurgitations. The number of herons that flew into the ranching area increased with the feeding period. The numbers of fish lost to predation by herons in 1997, 1998, and 1999 were estimated to be 0, 5,741, and 829, respectively.  相似文献   

Cottony leak of scarlet runner bean caused by <Emphasis Type="Italic">Pythium aphanidermatum</Emphasis>     

Kazumi Aoki  Motoaki Tojo  Ken Watanabe  Shihomi Uzuhashi  Makoto Kakishima 《Journal of General Plant Pathology》2007,73(6):408-410

Severe cottony leak was found on stems of scarlet runner bean (Phaseolus coccineus) in Ibaraki Prefecture in August 2006. The causal fungus was identified as Pythium aphanidermatum. The name cottony leak of scarlet runner bean (Watagusare-byo in Japanese) is proposed for this new disease.  相似文献   

Shell growth and age determination of the brackish water bivalve Corbicula japonica in Lake Shinji, Japan     

Kazuhiro  OSHIMA  Naoki  SUZUKI  Mikio  NAKAMURA  Kazumi  SAKURAMOTO 《Fisheries Science》2004,70(4):601-610

ABSTRACT:   Shell growth and age determination of Corbicula japonica were investigated from samples collected monthly at two sites and field growth experiments from November 1999 to November 2000 in Lake Shinji. Both specimens did not grow in winter when the water temperature was below 11°C, but grew rapidly from spring to early summer when the water temperature rose from 15°C to 30°C, before further growing slowly from summer to autumn after the main breeding period. The monthly marginal growth distribution of the samples indicated that the concentric groove on the shell surface was an annual growth ring. It is concluded that the age of C .  japonica in the i -ring group when the shell growth resumed in spring was i  + 0.5 ( i  = 1, 2, · · ·), because the first true growth ring was formed at 0–2 mm in shell length, which then faded with growth. Lee's phenomenon was observed in the age determination results, and thus the growth coefficient and the asymptotic shell length of the von Bertalanffy growth equation were not estimated from the samples. However, these parameters were estimated from the annual shell length increments of the experimental individuals, which were 0.331/year and 28.2 mm, respectively.  相似文献   

Expression of the sweet receptor protein, T1R3, in the human liver and pancreas     

Taniguchi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1311-1314

The expression of T1R3, a taste receptor essential for the perception of sweetness and umami-taste, was examined by immunohistochemistry to determine whether and where it may be localized in the liver and pancreas. In the liver, both immunopositive and immunonegative reactions were detected; bile ducts and intercalated portions of the bile ductules were immunopositive to T1R3, while arterioles and venules were immunonegative in interlobular connective tissue. In the hepatic lobule, all other cells including liver cells (hepatocytes) and bile capillaries were immunonegative. In the pancreas, all endocrine portions of the pancreas were immunonegative to T1R3. Within the exocrine portions, immunopositive reactions were detected in excretory duct cells, intercalated cells, and centroacinar cells. In contrast, acinar cells were immunonegative, as were vessels, lymph capillaries, nerve fibers, and connective tissue cells in the exocrine portions. The restricted localization of T1R3 in the duct cells of the liver and pancreas in the present study may indicate that T1R3 is involved in monitoring changes in the makeup of bile and pancreatic juices in the hepatic and pancreatic duct systems.  相似文献   

Differential expression of histochemical characteristics in the developing olfactory receptor cells in a flatfish, barfin flounder (Verasper moseri)     

Yamamoto Y  Mori M  Saito S  Amano M  Yamanome T  Taniguchi K  Oikawa T  Yamamori K  Taniguchi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1609-1611

Differentiation of the histochemical characteristics of the olfactory receptor cells (ORC) was examined by immunohistochemistry for protein gene product 9.5 (PGP 9.5) and calretinin (CR) and lectin histochemistry for Phaseolus vulgaris agglutinin-L (PHA-L) in the developing olfactory epithelium (OE) of the barfin flounder. PGP 9.5 immunoreactivity was diffuse and CR immunoreactivity was restricted at day 7, but these immunoreactivities became intense in the OE toward day 91. Crypt cells were first identified at day 56. PHA-L staining was faint at day 28, but became intense toward day 91. These findings suggest that PGP 9.5-immunopositive cells, CR-immunopositive cells, crypt cells and PHA-L-reactive cells differentiate independently in the developing OE and constitute subsets of the ORC in the OE.  相似文献   

The alternative food movement in Japan: Challenges,limits, and resilience of the teikei system     

Kazumi Kondoh 《Agriculture and Human Values》2015,32(1):143-153

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Histological and Lectin Histochemical Studies on the Olfactory and Respiratory Mucosae of the Sheep     

Dalia IBRAHIM  Nobuaki NAKAMUTA  Kazumi TANIGUCHI  Yoshio YAMAMOTO  Kazuyuki TANIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(3):339-346

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Influences of protein ingestion on glucagon‐like peptide (GLP)‐1‐immunoreactive endocrine cells in the chicken ileum     

Mohammad M. Monir  Kohzy Hiramatsu  Sachiko Matsumoto  Kei Nishimura  Chihiro Takemoto  Tomomi Shioji  Takafumi Watanabe  Kazumi Kita  Shinichi Yonekura  Sang Gun Roh 《Animal Science Journal》2014,85(5):581-587

Influences of a specific dietary nutrient on glucagon‐like peptide (GLP)‐1‐containing cells in the chicken intestine are not yet clear. Significance of dietary protein level on GLP‐1‐containing cells in the chicken ileum was investigated. Chickens fed control or experimental diets of varying protein levels were examined using immunohistochemical and morphometrical techniques. We show that the protein ingestion had an impact on the activities of GLP‐1‐immunoreactive cells in the chicken ileum. Weight gains declined with decreasing dietary crude protein (CP) levels, but no significant differences were detected in the daily feed intake and villous height. GLP‐1‐immunoreactive cells with a round or oval shape were frequently observed in the lower CP level groups (4.5% and 0%). Frequencies of occurrence of GLP‐1‐immunoreactive cells were 41.1 ± 4.1, 38.5 ± 4, 34.8 ± 3.1 and 34.3 ± 3.7 (cells/mm², mean ± SD) for dietary CP level of 18%, 9%, 4.5% and 0% groups, respectively and significant differences were recognized between the control and lower CP level groups (P < 0.05). Multiple regression analysis indicated a significant correlation between the daily protein intake and frequencies of occurrence of GLP‐1‐immunoreactive cells. The protein ingestion is one of the signals that influence GLP‐1‐containing cells in the chicken small intestine.  相似文献