Construction of a high-density reference linkage map of tea (Camellia sinensis)     

Fumiya Taniguchi  Kazumi Furukawa  Sakura Ota-Metoku  Nobuo Yamaguchi  Tomomi Ujihara  Izumi Kono  Hiroyuki Fukuoka  Junichi Tanaka 《Breeding Science》2012,62(3):263-273

A few linkage maps of tea have been constructed using pseudo-testcross theory based on dominant marker systems. However, dominant markers are not suitable as landmark markers across a wide range of materials. Therefore, we developed co-dominant SSR markers from genomic DNA and ESTs and constructed a reference map using these co-dominant markers as landmarks. A population of 54 F₁ clones derived from reciprocal crosses between ‘Sayamakaori’ and ‘Kana-Ck17’ was used for the linkage analysis. Maps of both parents were constructed from the F₁ population that was taken for BC₁ population. The order of most of the dominant markers in the parental maps was consistent. We constructed a core map by merging the linkage data for markers that detected polymorphisms in both parents. The core map contains 15 linkage groups, which corresponds to the basic chromosome number of tea. The total length of the core map is 1218 cM. Here, we present the reference map as a central core map sandwiched between the parental maps for each linkage group; the combined maps contain 441 SSRs, 7 CAPS, 2 STS and 674 RAPDs. This newly constructed linkage map can be used as a basic reference linkage map of tea.  相似文献   

Effect of annealing temperature on gelatinization of rice starch suspension as studied by rheological and thermal measurements   总被引：1，自引：0，他引：1  

Tsutsui K  Katsuta K  Matoba T  Takemasa M  Nishinari K 《Journal of agricultural and food chemistry》2005,53(23):9056-9063

The effect of annealing temperature (Ta) on the rheological behavior of 10 wt % rice starch suspension was investigated by the dynamic viscoelasticity, the differential scanning calorimetry (DSC), and the amount of leached out amylose and the swelling ratio of starch suspension. The rheological behaviors of the annealed samples are classified into three types in terms of Ta: Ta1, 48 and 55 degrees C, which are much lower than the gelatinization temperature, Tgel (=62 degrees C); Ta2, 58, 60, and 62 degrees C, which are almost the same as Tgel; and Ta3, 65, 68, 70, and 73 degrees C, which are much higher than Tgel. For the samples annealed at Ta2, the onset temperature of the storage and the loss moduli, G' and G', increased with increasing T(a), and G' and G" in the temperature range from 65 to 90 degrees C gradually increased though smaller than those for the nonannealed sample, the control. This can be understood by the partial gelatinization; i.e., the leached out amylose prevents further amylose from leaching out. The rheological property of the samples annealed at Ta1 is not so different from that of the control, and the samples annealed at Ta3 are almost gelatinized. The rheological behavior of starch suspension can be controlled by Ta.  相似文献   

Contextualizing farmers’ attitudes towards genetically modified crops     

Kazumi Kondoh  Raymond A. Jussaume Jr. 《Agriculture and Human Values》2006,23(3):341-352

Analyses of the role of technological development in agriculture are central to an understanding of social change in agri-food systems. The objective of this paper is to contribute to the formation of a broader perspective of how farmers are positioning themselves with respect to controversial agricultural technologies through an empirical analysis of Washington State farmers’ willingness or unwillingness to try Genetically Modified Organisms (GMOs) technology on their farms. The use of this type of biotechnology in farming has been criticized for its potential harmful effects on natural environments and socio-cultural systems, while proponents highlight the possibilities for increasing production with minimal use of other inputs. An analysis of the extent of farmers’ expressed willingness to use GMOs provides an opportunity to better understand how their diverse thoughts about controversial agricultural technologies are shaped not only by their own experiences but also by social context. The present study does this by analyzing data from a farm survey conducted on a random sample of farmers from across Washington State. The results show that the production practices farmers utilize and the market strategies they employ may be at least as useful as farmers’ socio-economic characteristics in explaining what types of farmers appear to be more or less interested in potentially using this technology. Furthermore, the relationship between level of formal education and willingness to use GMOs is not straightforward. It may hide differences between farmers with respect to where and how they received their formal education as well as the type(s) of knowledge they gained. It is argued that future research should recognize the diversity that exists in farmers’ interests vis-á-vis particular technologies and should also explore how these interests are shaped by farmers’ past and present social networks and life experiences. Kazumi Kondoh is a PhD candidate in the Department of Sociology at Washington State University. Her research interests include environmental policies, science and technology, and sustainable agriculture. Raymond Jussaume is an Professor and Chair, Community and Rural Sociology at Washington State University. His primary areas of interest are sociology of agriculture, development sociology, and political sociology.  相似文献   

Practicable group testing method to evaluate weight/weight GMO content in maize grains     

Mano J  Yanaka Y  Ikezu Y  Onishi M  Futo S  Minegishi Y  Ninomiya K  Yotsuyanagi Y  Spiegelhalter F  Akiyama H  Teshima R  Hino A  Naito S  Koiwa T  Takabatake R  Furui S  Kitta K 《Journal of agricultural and food chemistry》2011,59(13):6856-6863

Because of the increasing use of maize hybrids with genetically modified (GM) stacked events, the established and commonly used bulk sample methods for PCR quantification of GM maize in non-GM maize are prone to overestimate the GM organism (GMO) content, compared to the actual weight/weight percentage of GM maize in the grain sample. As an alternative method, we designed and assessed a group testing strategy in which the GMO content is statistically evaluated based on qualitative analyses of multiple small pools, consisting of 20 maize kernels each. This approach enables the GMO content evaluation on a weight/weight basis, irrespective of the presence of stacked-event kernels. To enhance the method's user-friendliness in routine application, we devised an easy-to-use PCR-based qualitative analytical method comprising a sample preparation step in which 20 maize kernels are ground in a lysis buffer and a subsequent PCR assay in which the lysate is directly used as a DNA template. This method was validated in a multilaboratory collaborative trial.  相似文献   

Cost sharing and sustainability of Pongsak Muang Fai irrigation system     

Tassanee Ounvichit  Masayoshi Satoh  Somboon Chantanusart  Kazumi Yamaoka 《Paddy and Water Environment》2006,4(2):81-88

The centuries-old, self-organizing Muang Fai community irrigation institution in northern Thailand has high potential in illustrating exemplary practices in irrigation cost sharing. This paper examines the cost sharing structure of the Pongsak Muang Fai Irrigation System in Mae Hong Son Province and its relationship with the system water management and sustainability. Results show that this primitive and high cost system accepts costs of the weir, the entire length of main ditch, not only the individually related ditch sections, and the management as common costs that should be equally shared so that it can gather sizeable membership to support the system. The capacity of this small scale run-of-the-river irrigation system with no river flow limitation is a simple summation of all determined farm intake capacities. The maintenance cost of the system every year depends on how much water should be diverted and conveyed, hence the use of “relative” scale of each farm intake capacity as the basis for sharing cost in the wet season paddy farming. Such structure is directly related to the water management under which every intake shall be served with continuous supply without permitting mid-canal water check-up and the members prefer to take collective action to keep enough water supply without leaving any room for suspicion of unfair water distribution. The direct relationship makes the members understand the purpose of payment and be willing to share the costs which are transparently estimated in easily understood terms and clearly de-aggregated into categories.  相似文献   

A Case of Olfactory Neuroblastoma Induced in A Rat by N-Nitrosobis(2-hydroxypropyl)amine     

Takagi M  Shiraiwa K  Kusuoka O  Tamura K 《Journal of toxicologic pathology》2010,23(2):111-114

N-nitrosobis(2-hydroxypropyl)amine (BHP) is a well-known carcinogen and induces tumors in various tissues. In the present paper, a case of olfactory neuroblastoma (ONB) induced in a rat by BHP is described. The tumor was observed in one out of 25 male rats that received 2000 ppm of BHP in drinking water from 6 to 18 weeks of age and were sacrificed at 26 weeks of age. Histologically, the tumor arose in the posterior nasal cavity and consisted of small round cells and elongate cells with scant basophilic cytoplasm. The neoplastic cells proliferated with compartmentalization into the lobules by fibrovascular septa. True rosettes, pseudorosettes and an intercellular fibrillar matrix were occasionally observed. Immunohistochemically, the tumor cells were positive for NF120/200 and β III-tubulin. These results indicate that the present tumor is the first case of ONB induced in a rat by BHP treatment.  相似文献   

Morphological characterization of skin ganglion-like cells in Djungarian hamsters (Phodopus sungorus)     

Kashida Y  Ishikawa K  Arai K  Mitsumori K 《Veterinary pathology》2003,40(5):548-555

Characteristic ganglion-like cell proliferation observed in the skin of Djungarian hamsters was investigated using 24 male and 24 female hamsters, 1-6 months of age, to examine the anatomic location of these ganglion-like cells and their morphologic features. One abdominal skin tumor composed of these cells and resembling proliferative fasciitis in humans was also examined. Skin ganglion-like cells were rarely observed in young animals but increased in number and extent with age, especially in males. These cells were frequently seen in the ventral and medial regions of the trunk and legs rather than in the dorsal and lateral regions. Light microscopic examination of these ganglion-like cells revealed abundant vesicular basophilic cytoplasm with delicate intracytoplasmic silver stain-positive fibrils. Ultrastructurally, these cells contained abundant rough endoplasmic reticulum and Golgi complexes with dilated cisternae; intracellular collagen fibrils were present within these cisternae. Heat shock protein 47, beta-tubulin, and androgen receptor were expressed in these cells. The morphologic features of cells of one tumor resembling human proliferative fasciitis were identical to those observed in ganglion-like cells. The results of the present study suggest that these ganglion-like cells are derived from intrinsic undifferentiated mesenchymal cells in the dermis or subcutaneous adipose tissue and that any tumor-like lesion they form should be regarded as an abnormal proliferative lesion of skin ganglion-like cells rather than as proliferative fasciitis or fibroma.  相似文献   

Timing and extent of hypersensitive response are critical to restrict local and systemic spread of Pepper mild mottle virus in pepper containing the L 3 gene     

Hiroyuki Hamada  Shigeharu Takeuchi  Akinori Kiba  Shinya Tsuda  Kazumi Suzuki  Yasufumi Hikichi  Tetsuro Okuno 《Journal of General Plant Pathology》2005,71(1):90-94

Amino acid changes in Pepper mild mottle virus (PMMoV) coat protein (CP) that enhance, decrease, or nullify the resistance-inducing activity in Capsicum plants carrying the L ³ gene have been identified. In this study, molecular events underlying the L ³ -gene-mediated resistance were analyzed through the expression of hypersensitive response (HR)-related genes, HSR203J-Cc and HIN1-Cc, and defense-related genes, PR1-Cc and PR4b-Cc, upon infection with PMMoV CP mutants. The expression kinetics of the genes correlated with the degree of restriction of virus distribution in the inoculated leaves. The results suggest that the timing and extent of HR are critical factors to restrict virus spread both locally and systemically in L ³ -gene-mediated resistance.The nucleotide sequence data reported are available in the DDBJ/GenBank/EMBL databases under accession numbers AB162220 (HSR203J-Cc), AB162221 (HIN1-Cc), AB162222 (PR1-Cc), and AB162223 (PR4b-Cc)  相似文献   

Survey of Cryptosporidium spp. and Giardia spp. infections in various animals at a zoo in Japan.     

M Matsubayashi  K Takami  Isao Kimata  Teruo Nakanishi  Hiroyuki Tani  Kazumi Sasai  Eiichiroh Baba 《Journal of zoo and wildlife medicine》2005,36(2):331-335

A total of 284 fecal samples of 89 species (43 mammalian species and 46 avian species) were examined for Cryptosporidium oocysts and Giardia cysts from 1999 to 2002. Each sample was collected at the zoo located at Osaka in Japan and examined by microscopy after performing the sucrose flotation method and by two immunofluorescent assay kits for detection of Cryptosporidium oocysts and Giardia cysts. Cryptosporidium spp. was found only in a raccoon dog (Nyctereutes procyonoides), and Giardia spp. was detected in a mandarin duck (Aix galericulata) and two ruddy shelducks (Tadorna ferruginea). In this study, the prevalences of these parasites were found to be low. However, these results suggested that the infected animals could serve as a source of contamination for surface water. This is the first report about the survey of Cryptosporidium spp. and Giardia spp. at a zoo in Japan.  相似文献   

Expression of porcine FSHbeta subunit promoter-driven herpes simplex virus thymidine kinase gene in transgenic rats     

Cai LY  Kato T  Ito K  Nakayama M  Susa T  Aikawa S  Maeda K  Tsukamura H  Ohta A  Izumi S  Kato Y 《The Journal of reproduction and development》2007,53(2):201-209

  相似文献