Inheritance mode of microsatellite DNA markers and their use for kinship estimation in kuruma prawn Penaeus japonicus   总被引：8，自引：0，他引：8  

TAKUMA  SUGAYA  MINORU  IKEDA  HIDESHI  MORI  NOBUHIKO  TANIGUCHI 《Fisheries Science》2002,68(2):299-305

The allelic inheritance mode of microsatellite DNA markers was examined using seven copulated wild females and their offspring. Five microsatellite loci, CSPJ002 *, CSPJ010 *, CSPJ012 *, CSPJ014 *, and CSPJ015 *, were used in the study. At almost all family/locus combinations, one sire was determined and distributions of genotypes in offspring were consistent with the Mendelian segregation ratio. Distributions of genotypes were consistent with the ratio after assuming a null allele at some loci. Consequently, the alleles of CSPJ002 * and CSPJ012 * were inherited following the Mendelian inheritance mode in every family; however, the null allele was expected in CSPJ010 *, CSPJ014 *, and CSPJ015 * in some families. Thus, these loci should be used carefully in population genetic analysis, but siblings could be detected in the dendrograms based on unweighted pair-group method using arithmetic averages (UPMGA).  相似文献   

Phytoplankton surveys off the southern Myanmar coast of the Andaman Sea: an emphasis on dinoflagellates including potentially harmful species     

Su-Myat  Maung-Saw-Htoo-Thaw  Kazumi Matsuoka  Khin-Ko-Lay  Kazuhiko Koike 《Fisheries Science》2012,78(5):1091-1106

A detailed list of dinoflagellate species for the Mergui Archipelago, off southern Myanmar, was compiled for the first time. This was based on surveys conducted in two seasons in pre- and post-monsoons, and on observations for both plankton and cyst assemblages. In the plankton samples, 57 and 26 dinoflagellate species were recorded, respectively, in the pre- and post-monsoons. Among the list from plankton and cyst samples, harmful species were found: red-tide species including Prorocentrum spp. and Alexandrium affine, and causative species of paralytic shellfish poisoning, e.g., Alexandrium tamiyavanichii and Gymnodinium catenatum, and of diarrhetic shellfish poisoning, e.g., Dinophysis spp. Therefore, although no incidences have been reported yet, attention must be paid to these harmful algal bloom (HAB) events in Myanmar where exploitation of fishery resources is drastically increasing. Other dinoflagellate compositions were also unique and may relate to the oceanographic system in this region. In the pre-monsoon, rather rare oceanic species of Ornithocercus spp. were found concurrently with neritic species, probably due to the extensive southwesterly current from the Indian Ocean. Throughout the seasons, diverse species of Protoperidinium were predominately recorded in both planktonic and sediment samples, indicating richness of prey organisms probably sustained by upwelling systems.  相似文献   

Estimation of daily urinary potassium excretion using urinary creatinine as an index substance in prepartum dairy cows     

Hideki ASAI  Noboru HAYASHI  Naoharu TAKAI  Yoshihisa YOSHIMURA  Yutaka NAKAMURA  Hiroomi YOKOTA  Kazumi KITA 《Animal Science Journal》2005,76(1):51-54

In the present study, the daily excretion of potassium (K) in urine (urinary K(UK)) was estimated from a 6 h urine sample using urinary creatinine (UC) as the index substance. All urine was collected from six pregnant Holstein cows at 6 h intervals for 24 h on 3 days of the 4th, 2nd and final week before the expected date of parturition. In total, 72 6 h urine samples were obtained. Daily UC excretion (mg/day per kg bodyweight (BW)) was almost the same for the three sampling days. Daily UC excretion varied among cows from 22.1 to 24.3 mg/day per kg BW with a mean of 22.8 mg/day per kg BW with no significant difference. Thus, daily UC excretion was confirmed to be constant throughout the prepartum period with no differences among individuals. The concentration ratios of K to creatinine ((UK mg/dL)/(UC mg/dL) (UK/UC)) correlated strongly to the hourly K excretions (mg/h per kg BW) (r = 0.952, P < 0.01) in the 6 h urine samples. The differences in the UK/UC ratio between sampling periods were not significant within each cow. Therefore, daily UK excretion (mg/day) can be estimated using the equation: daily UK excretion (mg/day) = daily UC excretion (mg/day per kg BW) × BW (kg) × 6 h urine sample UK/UC, where daily UC excretion can be a given value.  相似文献   

Changes of receptor mRNAs for oxytocin and estrogen during the estrous cycle in rat uterus   总被引：1，自引：0，他引：1  

Murata T  Narita K  Honda K  Higuchi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):707-712

Oxytocin receptor (OTR) mRNA levels increase dramatically near term and is potently stimulated by estrogen because increased OTR mRNA levels result from estrogen treatment in ovariectomized rat uterus. In this study, OTR, estrogen receptor (ER) alpha and ERbeta mRNA levels in the rat uterus during the estrous cycle were examined by quantitative RT-PCR. OTR mRNA levels during the estrous cycle began to increase on diestrus (P<0.05, vs value on estrus), reached maximal increase both in the morning (1000-1130 hr) and afternoon (1600-1630 hr) on proestrus (P<0.01, vs metestrus, diestrus and estrus) and then declined on estrus. In contrast ER alpha mRNA levels began to decrease on diestrus, reached statistical significance both in the morning and the afternoon on proestrus (P<0.01, vs metestrus, diestrus and estrus) and returned to the value of metestrus on estrus. ERbeta mRNA levels were low in the morning and the afternoon on proestrus (P<0.01, vs metestrus and estrus) and also returned to metestrus values on estrus. Treatments with estrogen for 3 days significantly decreased both ERalpha and ERbeta mRNA levels. It can be concluded from these results that during the estrous cycle, OTR mRNA levels in rat uterus predominantly increase at proestrus with a decrease in ERalpha and ERbeta mRNA levels, which is probably due to the increased estrogen levels in circulation before ovulation.  相似文献   

Recognition pattern of thyroglobulin autoantibody from hypothyroid dogs to tryptic peptides of canine thyroglobulin     

Tani H  Shimizu R  Sasai K  Baba E 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(10):1049-1056

Circulating thyroglobulin autoantibody (TgAA) was analyzed using the Western immunoblot for determination of the dominant epitopes recognized by TgAA on tryptic peptides of canine thyroglobulin (cTg) in hypothyroid dogs. TgAA was measured in hypothyroid dogs, non-hypothyroid dogs with skin diseases and clinically normal dogs. Five of the 7 hypothyroid dogs, 1 of the 8 dogs with skin diseases and 1 of the 4 normal dogs were positive for TgAA. Four of the 5 TgAA-positive hypothyroid dogs were Golden Retrievers, and 3 of them showed high antibody titers. The sera of TgAA positive-dogs reacted to several peptides, and their patterns varied from sample to sample. Sera from 3 dogs with high titers of TgAA reacted broadly to high molecular weight peptides ranging from 45 to 90 kDa. These Western immunoblot patterns of the sera were disappeared after pretreatment with sufficient amount of intact cTg. All serum samples of both TgAA positive dogs and negative controls reacted to low molecular weight peptides ranging from 15 to 20 kDa. These immunoblot patterns of the sera were not disappeared even after pretreatment with sufficient amount of intact cTg. These findings show the possibility that the epitopes recognized by TgAA depend upon individual dogs with hypothyroidism and these autoantibodies recognize conformational epitopes on the cTg molecule.  相似文献   

Purification and properties of β-galactosidase from Tilapia intestine: Digestive enzyme of Tilapia-X     

Akiko  YAMADA TANIGUCHI  Katsumi  TAKANO 《Fisheries Science》2004,70(4):688-694

ABSTRACT:   β-galactosidase of the intestine of Tilapia nilotica was purified by ammonium sulfate precipitation, followed by PAPTG-Sepharose 4B affinity chromatography, ethylenediamineetetraacetic acid ion-exchange chromatography, polyexchanger PBE 94 chromatofocusing, and Sephadex G-100 gel filtration. β-galactosidase was found to be a single band when examined by poly-acrylamide gel electrophoresis. The purifications of β-galactosidase were 27-fold from the crude extract. β-galactosidase showed optimum activity at pH 5.0 at 40°C, and was specifically found to be able to hydrolyze p -nitrophenyl β-galactopyranoside. It degrades galactan and agarose, and produces galactose. β-galactosidase was strongly inhibited by Hg²⁺ and PCMB. β-galactosidase is considered to be secreted by the upper and middle parts of the intestine and most of the activity was detected in the intestinal juice.  相似文献   

Effects of acrosomal conditions of frozen-thawed spermatozoa on the results of artificial insemination in Japanese Black cattle     

Kazumi KISHIDA  Mitsuhiro SAKASE  Kenta MINAMI  Miyuki M. ARAI  Reiko SYOJI  Namiko KOHAMA  Takayuki AKIYAMA  Akio OKA  Hiroshi HARAYAMA  Moriyuki FUKUSHIMA 《The Journal of reproduction and development》2015,61(6):519-524

The purposes of this study were to examine the relationship between male artificial insemination (AI) fertility and sperm acrosomal conditions assessed by new and conventional staining techniques and to identify possible reproductive dysfunctions causing low conception rates in AI using frozen-thawed spermatozoa with poor acrosomal conditions in Japanese Black bulls. We investigated individual differences among bulls in the results concerning (1) acrosomal conditions of frozen-thawed spermatozoa as assessed by not merely peanut agglutinin-lectin staining (a conventional staining technique) but also immunostaining of acrosomal tyrosine-phosphorylated proteins (a new staining technique), (2) routine AI using frozen-thawed spermatozoa as assessed by pregnancy diagnosis, (3) in vivo fertilization of frozen-thawed spermatozoa and early development of fertilized eggs as assessed by superovulation/AI-embryo collection tests and (4) in vitro fertilization of frozen-thawed spermatozoa with oocytes. The percentages of frozen-thawed spermatozoa with normal acrosomal conditions assessed by the abovementioned staining techniques were significantly correlated with the conception rates of routine AI, rates of transferable embryos in superovulation/AI-embryo collection tests and in vitro fertilization rates. These results are consistent with new suggestions that the distribution of acrosomal tyrosine-phosphorylated proteins as well as the acrosomal morphology of frozen-thawed spermatozoa are AI fertility-associated markers that are valid for the prediction of AI results and that low conception rates in AI using frozen-thawed spermatozoa with poor acrosomal conditions result from reproductive dysfunctions in the processes between sperm insemination into females and early embryo development, probably failed fertilization of frozen-thawed spermatozoa with oocytes.  相似文献   

Evaluation of color intensity enhanced by paprika as feed additive in goldfish and koi carp using computer-assisted image analysis   总被引：3，自引：0，他引：3  

Csaba  HANCZ  István  MAGYARY  Tamás  MOLNÁR  Shoh  SATO  Péter  HORN  Nobuhiko  TANIGUCHI 《Fisheries Science》2003,69(6):1158-1161

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Genetic structure of Chinese sucker population Myxocyprinus asiaticus in the Yangtze River based on mitochondrial DNA marker   总被引：1，自引：0，他引：1  

Yuhua  SUN  Siyang  LIU  Gang  ZHAO  Shunping  HE  Qingjiang  WU  Nobuhiko  TANIGUCHI  Qixin  YU 《Fisheries Science》2004,70(3):412-420

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Rod cells proliferate in the eel retina throughout life   总被引：1，自引：0，他引：1  

Yuri  OMURA  Kazumi  TSUZUKI  Mariko  SUGIURA  Kazumasa  UEMATSU  Katsumi  TSUKAMOTO 《Fisheries Science》2003,69(5):924-928

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