全文获取类型
收费全文 | 365篇 |
免费 | 12篇 |
国内免费 | 6篇 |
专业分类
林业 | 29篇 |
农学 | 14篇 |
79篇 | |
综合类 | 28篇 |
农作物 | 31篇 |
水产渔业 | 43篇 |
畜牧兽医 | 129篇 |
园艺 | 4篇 |
植物保护 | 26篇 |
出版年
2022年 | 1篇 |
2021年 | 6篇 |
2020年 | 5篇 |
2019年 | 6篇 |
2018年 | 5篇 |
2017年 | 7篇 |
2016年 | 11篇 |
2015年 | 12篇 |
2014年 | 16篇 |
2013年 | 24篇 |
2012年 | 27篇 |
2011年 | 30篇 |
2010年 | 11篇 |
2009年 | 15篇 |
2008年 | 29篇 |
2007年 | 32篇 |
2006年 | 27篇 |
2005年 | 20篇 |
2004年 | 25篇 |
2003年 | 10篇 |
2002年 | 19篇 |
2001年 | 11篇 |
2000年 | 10篇 |
1999年 | 6篇 |
1998年 | 1篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有383条查询结果,搜索用时 15 毫秒
101.
Sugita-Konishi Y Kobayashi K Sakanaka S Juneja LR Amano F 《Journal of agricultural and food chemistry》2004,52(17):5443-5448
We have previously reported that sialylglycopeptide (SGP) and its derivatives isolated from egg yolk had a preventive effect on Salmonella infection in vivo; however, their retention time in the gut was rather short. To improve on this, SGP was conjugated with carboxymethyl cellulose (CMC) or carboxymethyl dextran (CMD). The conjugates inhibited the binding of Salmonella enteritidis and Escherichia coli to Caco-2 cells. Infection experiments with mice revealed that the SGP-CMD conjugate (SGP-CMD) had a strong protective effect against Salmonella infection. A turnover experiment in mice administered with radiolabeled SGP-CMD showed that SGP-CMD was more slowly absorbed into the blood and thus remained longer in the intestinal tract than SGP. SGP-CMD itself did not influence the production of tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta, or nitrite ion (NO(2)(-)) by macrophages, although it suppressed that of TNF-alpha and NO(2)(-) in zymosan-treated macrophages, suggesting no causative effects of inflammation in SGP-CMD. SGP-CMD is potentially useful as a food ingredient with a preventive effect on Salmonella infection. 相似文献
102.
Kobayashi K Hattori M Hara-Kudo Y Okubo T Yamamoto S Takita T Sugita-Konishi Y 《Journal of agricultural and food chemistry》2004,52(18):5740-5746
Ovomucin glycopeptide (OGP) was prepared by size exclusion chromatography after Pronase digestion of hen egg ovomucin, and the binding of OGP to foodborne pathogens (Bacillus cereus,Clostridium perfringens, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enteritidis, Salmonella typhimurium, and Staphylococcus aureus) was investigaed. Binding assays with biotinylated bacteria as probes in microtiter plates showed that OGP bound to only E. coli O157:H7 among these foodborne pathogens. Periodate treatment markedly reduced the binding ability, indicating that E. coli O157:H7 bound to carbohydrate moieties of OGP. Lectin blot analysis with Maackia amurensis (MAA) and Sambucus nigra (SNA), which are specific for oligosaccharides containing sialic acid, revealed their binding sites in OGP were similar to the E. coli O157:H7 binding sites that were probed with biotinylated E. coli O157:H7 after Western blotting of OGP. Sialydase treatment of OGP abolished its ability to bind E. coli O157:H7, demonstrating that sialic acid played an important role in the binding. These results suggest that OGP has E. coli O157:H7-specific binding sites that consist of sialic acid. On the basis of these properties, OGP has the potential to be an ingredient with a protective effect against E. coli O157:H7 infection and to be a novel probe for the detection of E. coli O157:H7 in the food hygiene field. 相似文献
103.
Summary Resistance to bacterial wilt (Pseudomonas solanacearum) found in tuber-bearing Solanum species was transferred into a diploid potato breeding population. Simultaneous selections were made for agronomic characters, production of first division restitution (FDR) 2n pollen, and resistance. Diploid resistant genotypes were identified via inoculation with a virulent isolate (CIP-204) of race 3 of Pseudomonas solanacearum. These resistant diploid genotypes were crossed to susceptible tetraploid potatoes. An investigation was made to assess whether resistant diploid genotypes transmit resistance to bacterial wilt, which is a quantitatively inherited trait, to tetraploid potatoes via FDR 2n pollen. Tetraploid seedlings from 4x×2x crosses were inoculated with the same isolate CIP-204, and the percentage of surviving seedlings was scored. Some 4x×2x families from resistant diploid genotypes demonstrated a high level of survival rate. The transmission of bacterial wilt resistance was achieved by the use of FDR 2n pollen. It was speculated that a female x male interaction effect on the survival rate of the evaluated progeny may exist. Selecting proper 4x and 2x parents would be important for obtaining a higher frequency of transmission of resistance to bacterial wilt in the progeny. 相似文献
104.
Ayane Konta Shinichiro Ogawa Makoto Kimata Kazuo Ishii Yoshinobu Uemoto Masahiro Satoh 《Animal Science Journal》2020,91(1)
The performance of the two‐trait animal model that regards the first parity and later parities as two different traits in estimating genetic parameters for number of born alive (NBA) was examined using real and simulated data. Genetic parameters for NBA were estimated in purebred Landrace and Large White pigs using a single‐trait repeatability model (Model 1) that regards all parities as the same trait and a two‐trait animal model (Model 2) that regards the first and the later parities as different traits. For Model 2, the permanent environmental effect was fitted to only the records of the later parities. Heritability for NBA estimated using Model 1 was 0.12 for Landrace and 0.11 for Large White. Estimated heritability for NBA of the first parity and the later parities was 0.21 and 0.16, respectively, for Landrace; 0.18 and 0.16, respectively, for Large White obtained using Model 2, and higher than those in both breeds obtained using Model 1. Further results based on data simulated using the Monte Carlo method suggest that estimated additive genetic variance could be more biased using Model 2 than Model 1. 相似文献
105.
Tetsuya Takatsu Yuusuke Suzuki Akiko Shimizu Kazuo Imura Yuko Hiraoka Naonobu Shiga 《Fisheries Science》2007,73(1):142-155
To clarify the feeding strategy of pelagic larvae of stone flounder in Mutsu Bay, the dietary composition and pray size was
investigated from February to April during 1989–1999. Diets were compared with the numerical and volumetric compositions and
frequency of occurrence of each prey species. Mensuration formulae were applied to estimate individual prey volume in the
diet, while the length of planktonic species was measured from net and water samples. Prey shapes were assumed as sphere,
cylinder, ellipsoid, pyramid, two elliptical cones, or a combination of ellipsoid and cylinder. Prey-size range increased
as the larvae grew. Preflexion larvae fed mainly on copepod nauplii. Flexion and postflexion larvae ingested primarily appendicularians,
with a suggestion that these larvae might depend on some parts of the microbial food web. Low frequencies of flexion and postflexion
larvae with empty guts (1.7 and 1.4%, respectively) might be derived from feeding on slow-swimming appendicularians. From
a size comparison between ‘house’-like organ length and trunk length of the appendicularian Oikopleura sp., almost all house-like organs with trunks in the larval diet were nonexpanded ‘house rudiments’, not expanded ‘houses’.
Thus, stone flounder larvae may not chew the houses, but swallow the house rudiments with trunks. 相似文献
106.
How nitrogen (N) cycling is regulated and how environmental change affects it are major study questions in forest ecology,
because N availability often limits the primary production of plants in many forest ecosystems. These are being extensively
highlighted because of growing concerns regarding chronic and elevated N deposition in forest ecosystems on a global scale.
Until now, N cycling has been mainly documented in association with various environmental factors other than microbial communities.
However, with the recent rapid development in culture-independent molecular-based techniques, microbial ecologists have discovered
that alterations in N cycling are highly associated with alternations in microbial communities through changes in either resource
supplies or processing rates. In this review, we describe nitrification as a key N cycling process and present general approaches
to associate the nitrification process with the nitrifying community in forest soils. Furthermore, we briefly summarize currently
available information about the relationship between the process and nitrifying community dynamics in soil. We suppose that
linking N cycling processes with microbial community dynamics provides a deeper insight into the mechanisms regulating N cycling
in forest ecosystems. 相似文献
107.
In 2009, chlorotic mottle and necrosis were observed on chrysanthemums (cv. Jimba) in Aomori Prefecture, Japan. A virus was
isolated from the chrysanthemum plants by serial local-lesion transfer. The symptoms exhibited by the test plants, the particle
morphology, the features of the protein and the potential for transmission by thrips were similar to those for Impatiens necrotic spot virus (INSV). The partial nucleotide sequences of the nucleocapsid protein gene and the 3′-untranslated sequence of the S RNA shared
99% identity with that of an INSV isolate. This report is the first of INSV infection of chrysanthemums in Japan. 相似文献
108.
109.
110.
Okada H Iwamaru Y Imamura M Masujin K Matsuura Y Shimizu Y Kasai K Takata M Fukuda S Nikaido S Fujii K Onoe S Mohri S Yokoyama T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1465-1471
Bovine spongiform encephalopathy (BSE) is a fatal neurodegenerative disorder of cattle characterized by accumulation of the disease-associated prion protein (PrP(Sc)) in the central nervous system (CNS). The immunohistochemical patterns and distribution of PrP(Sc) were investigated in the CNS, brains, and spinal cords of 7 naturally occurring BSE cases confirmed by the fallen stock surveillance program in Japan. No animals showed characteristic clinical signs of the disease. Coronal slices of 14 different brain areas in each case were immunohistochemically analyzed using an anti-prion protein antibody. Immunolabeled PrP(Sc) deposition was widely observed throughout each brain and spinal cord. Intense PrP(Sc) deposition was greater in the thalamus, brainstem, and spinal cord of the gray matter than in the neocortices. The topographical distribution pattern and severity of PrP(Sc) accumulation were mapped and plotted as immunohistochemical profiles of the different brain areas along the caudal-rostral axis of the brain. The distribution pattern and severity of the immunolabeled PrP(Sc) in the CNS were almost the same among the 7 cases analyzed, suggesting that the naturally occurring cases in this study were at the preclinical stage of the disease. Immunohistochemical mapping of the PrP(Sc) deposits will be used to clarify the different stages of BSE in cattle. 相似文献