首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   518篇
  免费   13篇
  国内免费   7篇
林业   29篇
农学   14篇
基础科学   1篇
  93篇
综合类   30篇
农作物   31篇
水产渔业   66篇
畜牧兽医   233篇
园艺   4篇
植物保护   37篇
  2022年   10篇
  2021年   27篇
  2020年   9篇
  2019年   6篇
  2018年   5篇
  2017年   7篇
  2016年   15篇
  2015年   25篇
  2014年   28篇
  2013年   32篇
  2012年   28篇
  2011年   36篇
  2010年   15篇
  2009年   18篇
  2008年   41篇
  2007年   37篇
  2006年   32篇
  2005年   26篇
  2004年   33篇
  2003年   20篇
  2002年   32篇
  2001年   15篇
  2000年   12篇
  1999年   6篇
  1998年   1篇
  1997年   5篇
  1996年   4篇
  1995年   2篇
  1994年   3篇
  1993年   3篇
  1992年   1篇
  1991年   1篇
  1990年   1篇
  1955年   1篇
  1953年   1篇
排序方式: 共有538条查询结果,搜索用时 15 毫秒
461.
We performed a standing hand-assisted laparoscopic ovariectomy in a draft mare that presented with high serum anti-Müllerian hormone (AMH) level and had an enlarged single cystic ovary. Histopathological examination revealed no tumor cell proliferation in the ovary, but the presence of a large ovarian cyst was confirmed. In the diagnosis of abnormal ovaries in mares, a comprehensive assessment should be performed, including the monitoring of ovarian morphology and biomarkers over time, to determine the disease prognosis and treatment plan. The case of this mare with a nonneoplastic abnormal ovary and increased serum AMH level was rare. We suggest that standing hand-assisted laparoscopic ovariectomy is useful for the removal of large ovaries in draft mares.  相似文献   
462.
The incidence of copper-associated hepatitis in Labrador retriever in Japan has not been examined. This study examined the genotype frequencies of ATP7B:c.4358G>A, a mutation responsible for copper-associated hepatitis, and ATP7A:c.980C>T, a modifier of this disease, in Labrador retrievers of guide dog associations in Japan. Genetic material was collected by buccal swabs from 253 Labrador retrievers and genotyping was performed for the ATP7B and ATP7A mutations. The gene frequency was 0.107 for ATP7B:c.4358A. For ATP7A:c.980C, the gene frequencies were 0.703 in females and 0.368 in males. In this study, we established genotyping methods for the ATP7B:c.4358G>A and ATP7A:c.980C>T mutations. Based on the genotyping results, the risk of copper-associated hepatitis in the study population was 0.80% in males and 1.05% in females.  相似文献   
463.
Oarfish (Regalecus russelii Cuvier) are mesopelagic fish with little known about their life history. Oarfish live in deep water, making it difficult for researchers to collect specimens; thus, records of their parasitic helminths are limited. Two plerocercoids were found for the first time in an oarfish stranded on the coast of Akita Prefecture, Japan. These plerocercoids were identified as Clistobothrium sp. RR-1 using morphological and molecular analyses. It was revealed that oarfish represent one of the intermediate hosts of the genus Clistobothrium, and large sharks are the definitive hosts for these parasites.  相似文献   
464.
价值链分析法是研究农村开发问题的有效手段。文中通过价值链分析方法构成要素之一的人力资源管理,对技术开发的可能性进行了分析和探讨。经研究证明了根据不具备利害关系的农牧民提出的想法和点子进行技术开发的有效性。以下两种结果是基于农牧民的知识与智慧进行技术开发的成功案例。在黑龙江省盐碱土壤改良中采用了来自水田地区的技术点子。具体内容是通过稻壳防止毛细管作用,种植苜蓿。在内蒙古将牧民的技术点子运用在蔬菜栽培方面。具体内容为利用畜粪发酵热的有机蔬菜种植栽培系统的开发。研究还发现,在构建广域的资源循环系统的过程中对于表现出非协助态度的农牧民,行为经济学的解决方法较有效。研究结果中,有一项有机水稻栽培系统是根据半干旱地区的农牧民提出的技术点子进行设计的,内容为利用羊粪浮力的省力型有机物质投入方法。本研究的研究结论证明了在面对复杂的地域问题研究时,促进包括不具备厉害关系的各地农牧民的参与,及时发现并积极发挥其创意点子的重要性。  相似文献   
465.
Isozyme patterns were examined to characterize biological species ofArmillaria in Japan. Of 25 different enzymes tested, glutamate dehydrogenase (GDH), lactate dehydrogenase (LDH), and aspartate aminotransferase (AAT) showed the most stable and reliable activities. These isozyme patterns showed little variability between haploid and diploid hyphae even under different cultural conditions among isolates tested. From the results, six Japanese biological species were clearly characterized through combination of GDH and LDH isozymes. There were no differences on the whole in isozyme patterns among European biological species ofArmillaria.  相似文献   
466.
Collapse-type shrinkage is one of highly refractory drying defects in low-medium density plantation-grown eucalypt wood used as solid wood products. Basic density (BD), microfibril angle (MFA), double fibre cell wall thickness (DWT), proportion of ray parenchyma (RP), unit cell wall shrinkage, total shrinkage and residual collapse, which are associated with collapse-type shrinkage characteristics, were investigated by using simple regression method for three species of collapse-susceptible Eucalyptus urophyll, E. grandis and E.urophyllaxE.grandis, planted at Dong-Men Forest Farm in Guangxi autonomous region, China. The results indicated that : unit cell wall shrinkage had a extremely strong positive correlation with BD, moderately strong positive correlation with DWT, and a weakly or moderately negative correlation with RP and MFA; total shrinkage was positively correlated with BD, DWT and RP and negatively related to MFA, but not able to be predicted ideally by any examined factors alone owing to lower R^2 value (R^2≤0.5712); residual collapse was negatively correlated with BD and DWT, linearly positively correlated with MFA, and had strongly positive linear correlation with RP. It is concluded that BD can be used as single factor (R^2≥0.9412) to predicate unit cell wall shrinkage and RP is the relatively sound indicator for predicting residual collapse  相似文献   
467.
468.
Potato has a variety of reproductive uniquenesses besides its clonal propagation by tubers. These traits are controlled by a different kind of genetic control. The reproductive information has been applied to enable interspecific hybridization to enhance valuable traits, such as disease and pest resistances, from the tuber-bearing Solanum gene pool. While progress has been made in potato breeding, many resources have been invested due to the requirements of large populations and long time frame. This is not only due to the general pitfalls in plant breeding, but also due to the complexity of polyploid genetics. Tetraploid genetics is the most prominent aspect associated with potato breeding. Genetic maps and markers have contributed to potato breeding, and genome information further elucidates questions in potato evolution and supports comprehensive potato breeding. Challenges yet remain on recognizing intellectual property rights to breeding and germplasm, and also on regulatory aspects to incorporate modern biotechnology for increasing genetic variation in potato breeding.  相似文献   
469.
Brown seaweed lipids from Undaria pinnatifida (Wakame), Sargassum horneri (Akamoku), and Cystoseira hakodatensis (Uganomoku) contained several bioactive compounds, namely, fucoxanthin, polyphenols, and omega-3 polyunsaturated fatty acids (PUFA). Fucoxanthin and polyphenol contents of Akamoku and Uganomoku lipids were higher than those of Wakame lipids, while Wakame lipids showed higher total omega-3 PUFA content than Akamoku and Uganomoku lipids. The levels of docosahexaenoic acid (DHA) and arachidonic acid (AA) in liver lipids of KK-A(y) mouse significantly increased by Akamoku and Uganomoku lipid feeding as compared with the control, but not by Wakame lipid feeding. Fucoxanthin has been reported to accelerate the bioconversion of omega-3 PUFA and omega-6 PUFA to DHA and AA, respectively. The higher hepatic DHA and AA level of mice fed Akamoku and Uganomoku lipids would be attributed to the higher content of fucoxanthin of Akamoku and Uganomoku lipids. The lipid hydroperoxide levels of the liver of mice fed brown seaweed lipids were significantly lower than those of control mice, even though total PUFA content was higher in the liver of mice fed brown seaweed lipids. This would be, at least in part, due to the antioxidant activity of fucoxanthin metabolites in the liver.  相似文献   
470.
ABSTRACT: Atypical bovine spongiform encephalopathy (BSE) has recently been identified in Europe, North America, and Japan. It is classified as H-type and L-type BSE according to the molecular mass of the disease-associated prion protein (PrPSc). To investigate the topographical distribution and deposition patterns of immunolabeled PrPSc, H-type BSE isolate was inoculated intracerebrally into cattle. H-type BSE was successfully transmitted to 3 calves, with incubation periods between 500 and 600 days. Moderate to severe spongiform changes were detected in the cerebral and cerebellar cortices, basal ganglia, thalamus, and brainstem. H-type BSE was characterized by the presence of PrP-immunopositive amyloid plaques in the white matter of the cerebrum, basal ganglia, and thalamus. Moreover, intraglial-type immunolabeled PrPSc was prominent throughout the brain. Stellate-type immunolabeled PrPSc was conspicuous in the gray matter of the cerebral cortex, basal ganglia, and thalamus, but not in the brainstem. In addition, PrPSc accumulation was detected in the peripheral nervous tissues, such as trigeminal ganglia, dorsal root ganglia, optic nerve, retina, and neurohypophysis. Cattle are susceptible to H-type BSE with a shorter incubation period, showing distinct and distinguishable phenotypes of PrPSc accumulation.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号