Spatial and temporal variability of unicellular diazotrophic cyanobacteria in the eastern Seto Inland Sea     

Ryoya Hashimoto  Hiroyasu Watai  Kazutaka Miyahara  Yoshihiko Sako  Takashi Yoshida 《Fisheries Science》2016,82(3):459-471

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<Emphasis Type="Italic">Pantoea ananatis</Emphasis> strains are differentiated into three groups based on reactions of tobacco and welsh onion and on genetic characteristics     

Kazutaka Kido  Masaru Hasegawa  Hiroyuki Matsumoto  Masaki Kobayashi  Yuichi Takikawa 《Journal of General Plant Pathology》2010,76(3):208-218

Ninety-six strains of Pantoea ananatis were isolated from 14 plant species including melon, rice, tea and other crops of economic importance. They were classified into three groups (group I, II, III) based on a welsh onion stabbing assay, tobacco infiltration test, and polymerase chain reaction to detect indole acetic acid (IAA) biosynthesis genes (iaaM and iaaH) and a cytokinin biosynthesis gene (etz). Group Ι strains were characterized as causing significant blight symptom on welsh onion and inducing a hypersensitive response (HR)-like reaction on tobacco leaves after 36–48 h and encompassed 20 isolates from foxtail millet, hydrangea, pineapple, river water and rice. These 20 isolates did not possess iaaM, iaaH, or etz genes. Group II, consisting of 34 melon isolates, harbored iaaM, iaaH and etz genes, but did not cause either blight on welsh onion or HR-like reaction on tobacco. Group III strains did not have the iaaM, iaaH, and etz genes, nor did they cause any reaction on welsh onion or tobacco. The 42 strains in group III were isolated from bamboo grass, Chinese silver grass, citrus, dogwood, melon, mugwort, silk tree, sweet corn, tea and welsh onion. Representative strains of the three groups were tested for pathogenicity on melon and rice. Group Ι strains caused palea browning on rice but not internal fruit rot on melon. On the contrary, group II strains did not cause disease on rice but caused internal fruit rot on melon. Group III strains were not pathogenic on rice or melon. These results suggested that the host range of P. ananatis may be predicted by the reactions of welsh onion and tobacco and detection of iaaM, iaaH and etz genes. These tools may serve as rapid tests to identify the pathogenicity groups of P. ananatis.  相似文献   

Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer <Emphasis Type="Italic">Takifugu rubripes</Emphasis> juveniles     

Kogen Okita  Engkong Tan  Hina Satone  Shigeharu Kinoshita  Shuichi Asakawa  Daisuke Ojima  Hideki Yamazaki  Kazutaka Sakiyama  Tomohiro Takatani  Osamu Arakawa  Atsushi Hagiwara  Yoshitaka Sakakura 《Fisheries Science》2017,83(3):401-412

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Synthesis and insecticidal/acaricidal activity of novel 3‐(2,4,6‐trisubstituted phenyl)uracil derivatives     

Kazuo Yagi  Kazuhiko Akimoto  Norihiko Mimori  Toshiro Miyake  Masaki Kudo  Kazutaka Arai  Shigeru Ishii 《Pest management science》2000,56(1):65-73

A series of novel 3‐(2,4,6‐trisubstituted phenyl)uracil derivatives has been synthesised and assayed for insecticidal/acaricidal activity. The assay indicated certain requirements for optimal insecticidal activity, which can be summarised as follows: (a) the substituents on the phenyl ring should possess hydrophobicity and electron‐withdrawing properties, and the sum of their volumes determines the level of activity; (b) the substituent at the 6‐position on the uracil ring should also possess electron‐withdrawing properties and hydrophobicity, together with the correct volume; (c) the 1‐position on the uracil ring should be unsubstituted for activity against Nephotettix cincticeps and Epilachna vigintioctopunctata, but substituents with length C3 to C4 may be optimal for activity against Tetranychus urticae; (d) certain substituents at the 5‐position of the uracil ring give activity against E vigintioctopunctata and T urticae, but not against N cincticeps; (e) a thiocarbonyl group at the 2‐position of the uracil ring is less effective than a carbonyl group. Of the compounds assayed, 3‐(2,6‐dichloro‐4‐trifluoromethylphenyl)‐6‐trifluoromethyluracil showed high activity against all the species assayed. © 2000 Society of Chemical Industry  相似文献   

Phytoplankton uptake and growth rate in the Japanese egg cockle Fulvia mutica     

Sou Nagasoe  Tetsuji Hisada  Goh Nishitani  Hiroyuki Takeuchi  Kazutaka Sakiyama 《International Aquatic Research》2019,11(4):359-375

To clarify the relationship between the quantity of food ingested by and the growth rate of the Japanese egg cockle Fulvia mutica (Reeve), we conducted a laboratory breeding experiment for 2 weeks and estimated the chlorophyll a (chl-a) concentrations in water and the increments in shell length and soft-body weight of this species under five chl-a concentrations. Moreover, we compared the relationship between cockle growth (changes in soft-body weight and shell length) and their feeding environment observed in the laboratory experiment with the results of a field investigation conducted at two sites in the Sea of Japan, Kumihama Bay (35°38′5″ N, 134°54′00″ E) and Kunda Bay (35°33′30″ N, 135°15′4″ E). The changes in soft-body weight were similar in both laboratory and field investigations, but those in shell length were not. We, therefore, considered shell length changes as unsuitable for evaluating the relationship between growth and feeding in F. mutica. Based on the changes in soft-body weight, it was possible to classify the feeding environment of this species into the following three types: (1) < 1.52 μg chl-a L−1, negative feeding environment for cockle growth; (2) 1.52–5.71 μg chl-a L−1, neutral feeding environment for cockle growth; (3) > 5.71 μg chl-a L−1, positive feeding environment for cockle growth (growth increased with increasing chl-a concentration up to about 11 μg chl-a L−1). These results indicate that maintaining chl-a concentration in the breeding water within 5.71–11 μg chl-a L−1 is desirable for rearing Japanese egg cockle.  相似文献   

Quantitative estimation of the predation of marine ranched striped jack <Emphasis Type="Italic">Pseudocaranx dentex</Emphasis> by herons     

Harunao Nibe  Naoki Suzuki  Kazutaka Sakiyama  Shinsuke Morioka  Atsushi Ohno 《Fisheries Science》2010,76(3):473-480

The Goto station of the Japan Sea-Farming Association conducted marine ranching experiments with striped jack Pseudocaranx dentex from 1988 to 1999 in the Goto Islands, Nagasaki, Japan. Marine ranching is a stocking method where released fish are fed for some period after release in order to decrease initial mortality from starvation. Ranched fish were fed for 0, 90, and 15 days after stocking in 1997, 1998, and 1999, respectively. During the experiment, herons (grey heron Ardea cinerea, black-crowned night heron Nycticorax nycticorax, and great egret Egretta alba) were often observed preying on released fish in the ranching area. The present study estimated the number of fish preyed upon by herons during observation periods in 1997, 1998, and 1999 from the number of herons that flew into the ranching area and the predation rate on ranched fish by herons. The former was counted during sightings. The latter was estimated from the sighting observations and heron regurgitations. The number of herons that flew into the ranching area increased with the feeding period. The numbers of fish lost to predation by herons in 1997, 1998, and 1999 were estimated to be 0, 5,741, and 829, respectively.  相似文献   

Determination of plasma vitamin C concentration in fattening cattle     

Lin HAIYING  Licza PADILLA  Kazutaka YOSHIMATSU  Tohru MATSUI  Masayuki KITAGAWA  Hideo YANO 《Animal Science Journal》2003,74(1):7-10

Plasma vitamin C (ascorbic acid + dehydroascorbic acid) concentration is a good index of the nutritional status of vitamin C. However, the methodologies for storage and analyses have not been investigated in bovine plasma. The validity of an analytical method for bovine plasma using high performance liquid chromatography (HPLC) with a spectrophotometric detector was examined. Exogenous dehydroascorbic acid was almost completely converted to ascorbic acid during the preparation for analysis with a reducing reagent, dithioerythritol. The analytical recoveries of ascorbic acid were high. Ascorbic acid was not detected after treatment with ascorbic acid oxidase. Thus, the specificity of this method is considered to be high. Although vitamin C was stable in plasma treated by dithioerythritol at ?20°C for 6 days, vitamin C in untreated plasma significantly decreased during 3‐day storage at ?20°C. These results indicate that the HPLC method is suitable for the determination of plasma vitamin C in cattle and that the storage conditions are important for determination of plasma vitamin C. Plasma vitamin C concentration ranged between 1.49 mg/L and 3.33 mg/L in fattening cattle. This result suggests that fattening cattle show large individual variation in plasma vitamin C concentration.  相似文献   

Prevalence of Giardia intestinalis and other zoonotic intestinal parasites in private household dogs of the Hachinohe area in Aomori prefecture, Japan in 1997, 2002 and 2007     

Naoyuki Itoh  Kazutaka Kanai  Yasutomo Hori  Fumio Hoshi  Seiichi Higuchi 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(4):305-308

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Pathogenicity evaluation of Ralstonia pseudosolanacearum race 4 on ginger by leaf-clipping inoculation     

Iiyama  Kazuhiro  Imamura  Momoka  Inoue  Tomomi  Kyaw  Htet Wai Wai  Yano  Kazutaka  Horita  Mitsuo  Tsuchiya  Kenichi  Furuya  Naruto 《Journal of General Plant Pathology》2021,87(5):269-272

Journal of General Plant Pathology - An easy, rapid method has been needed to test the pathogenicity of strains of Ralstonia pseudosolanacearum, the cause of serious bacterial wilt on ginger...  相似文献   

Effect of Biodegradable Gelatin β-Tri Calcium Phosphate Sponges Containing Mesenchymal Stem Cells and Bone Morphogenetic Protein-2 on Equine Bone Defect     

Nao Tsuzuki  Shougo Nakao  Jong-pil Seo  Kazutaka Yamada  Shingo Haneda  Hidefumi Furuoka  Yasuhiko Tabata  Naoki Sasaki 《Journal of Equine Veterinary Science》2014

This study evaluated the osteoanagenetic effects of administering biodegradable gelatin β-tri calcium phosphate sponges containing mesenchymal stem cells (MSCs) and bone morphogenetic protein-2 (BMP-2) on equine bone defect. Six healthy Thoroughbred horses were used in this study. Horses were anesthetized, and skin incisions were made on all the limbs. Splint bones were exposed and a 1-cm bone defect was created in each exposed bone. Gelatin β-tri calcium phosphate sponges containing MSC and BMP-2 (MSC + BMP-2 sponge), MSC only (MSC sponge), BMP-2 only (BMP-2 sponge), or saline (saline sponge) were implanted into each bone defect at random. Defects were monitored for 16 weeks by radiography followed by computed tomography (CT) and histologic analyses. At 16 weeks, radiographic scores of MSC + BMP-2 sponge-treated defects were significantly higher than those of saline-treated defects (P = .027). Moreover, the CT value of the MSC + BMP-2 sponge group was significantly higher than that of the other groups (P = .027; P = .046; and P = .027, respectively), and the histologic score of the MSC + BMP-2 sponge group was significantly greater than that of the saline sponge group (P = .041). We conclude that MSC + BMP-2 sponge administration to bone defects accelerates bone regeneration in equines.  相似文献