Anti-inflammatory potency of oral disulfiram compared with dexamethasone on endotoxin-induced uveitis in rats     

Kanai K  Itoh N  Ito Y  Nagai N  Hori Y  Chikazawa S  Hoshi F  Higuchi S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(4):517-520

To investigate potency of oral disulfiram (DSF) compared with that of dexamethasone (Dexa), on endotoxin-induced uveitis (EIU) in rats. The oral administration with 750 mg/kg DSF suppressed the number of inflammatory cells, protein concentration, and levels of tumor necrosis factor (TNF)-α, Nitric oxide (NO) and prostaglandin (PG) E2 in the aqueous humor and improved the histiologic status of the ocular tissue at 24 hr after lipopolysaccharide (LPS) injection. The anti-inflammatory potency of DSF oral administration was as strong as that observed with 0.5 mg/kg Dexa in the present study. The results suggest that DSF might pave the way for a novel therapeutic agent for the management of uveitis.  相似文献   

Quantification of genetically modified soybean by quenching probe polymerase chain reaction     

Tani H  Noda N  Yamada K  Kurata S  Tsuneda S  Hirata A  Kanagawa T 《Journal of agricultural and food chemistry》2005,53(7):2535-2540

Quenching probe (QProbe) polymerase chain reaction (PCR) is a simple and cost-effective real-time PCR assay in comparison with other real-time PCR assays such as the TaqMan assay. We used QProbe-PCR to quantify genetically modified (GM) soybean (Roundup Ready soybean). We designed event-specific QProbes for Le1 (soy endogenous gene) and RRS (recombinant gene), and we quantified certified reference materials containing 0.1, 0.5, 1, 2, and 5% GM soybean. The TaqMan assay was also applied to the same samples, and the results were compared. The accuracy of QProbe-PCR was similar to that of TaqMan assay. When GM soybean content was 0.5% or more, the relative standard deviations of QProbe-PCR were less than 20%. QProbe-PCR is sensitive enough to monitor labeling systems and has acceptable levels of accuracy and precision.  相似文献   

Hemolymph levels of vertebrate-type steroid hormones in female kuruma prawn Marsupenaeus japonicus (Crustacea: Decapoda: Penaeidae) during natural reproductive cycle and induced ovarian development by eyestalk ablation   总被引：1，自引：0，他引：1  

Takuji  OKUMURA  Kazutaka  SAKIYAMA 《Fisheries Science》2004,70(3):372-380

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The Effects of Inclination (Up and Down) of the Treadmill on the Electromyogram Activities of the Forelimb and Hind limb Muscles at a Walk and a Trot in Thoroughbred Horses     

Toshiyuki TAKAHASHI  Akira MATSUI  Kazutaka MUKAI  Hajime OHMURA  Atsushi HIRAGA  Hiroko AIDA 《Journal of Equine Science》2014,25(4):73-77

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Effect of Platelet-Rich Plasma-Incorporated Gelatin Hydrogel Microspheres and Subchondral Drilling on Equine Cartilage Defects     

Nao Tsuzuki  Noe Oshita  Jong-pil Seo  Kazutaka Yamada  Shingo Haneda  Hidefumi Furuoka  Yasuhiko Tabata  Naoki Sasaki 《Journal of Equine Veterinary Science》2014

We evaluated the effect of platelet-rich plasma-incorporated gelatin hydrogel microsphere (PRP/GM) together with subchondral drilling for cartilage regeneration in horses. Cartilage defects (diameter, 4.5 mm; depth, 3.0 mm) were created in both third carpal bones of six Thoroughbred horses. A subchondral hole was drilled at the center of this cartilage defect (diameter, 2.0 mm; depth, 35 mm) in one randomly selected carpal joint (test joint), whereas no subchondral hole was made in the contralateral joint (control joint). Thereafter, the test joint was injected with PRP/GM and the control joint with saline-infused GM. Additional injections of PRP/GM were given 14 and 28 days after surgery. After 16 weeks, histologic analysis was conducted. The macroscopic and histologic scores of the test group were significantly higher than those of the control group (P < .05). These data indicated that the application of PRP/GM together with subchondral drilling accelerated cartilage regeneration in horses.  相似文献   

Change in Serum Ferritin Concentration in Experimentally Induced Anemia of Chronic Inflammation in Dogs     

Seishiro CHIKAZAWA  Takafumi NAKAZAWA  Yasutomo HORI  Fumio HOSHI  Kazutaka KANAI  Naoyuki ITO  Koichi ORINO  Kiyotaka WATANABE  Seiichi HIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(11):1419-1426

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The survival of multidrug‐resistant bacteria in thermophilic and mesophilic anaerobic co‐digestion of dairy manure and waste milk     

Nilmini Beneragama  Masahiro Iwasaki  Suraju A. Lateef  Takaki Yamashiro  Ikko Ihara  Kazutaka Umetsu 《Animal Science Journal》2013,84(5):426-433

Anaerobic digestion is considered as a promising method to manage animal waste with antibiotic‐resistant bacteria. Current research was conducted to investigate the survival of multidrug‐resistant bacteria (MDRB) resistant to three groups of antibiotics: (i) cefazolin, neomycin, vancomycin, kanamycin (group 1); (ii) penicillin, oxytetracycline, ampicillin, streptomycin (group 2); and (iii) cefazolin, neomycin, vancomycin, kanamycin, penicillin, oxytetracycline, ampicillin, streptomycin (group 3), in anaerobic digestion of dairy manure and co‐digestion of dairy manure and waste milk at 37°C and 55°C for 22 days, respectively. The population densities of three groups of MDRB on peptone, tryptone, yeast and glucose agar plates incubated at 30°C for 7 days before and after digestion showed 100% destruction in both digestates at thermophilic temperature. Overall reduction of more than 90% of three groups of MDRB was observed in mesophilic digestion with no significant differences (P > 0.05) between manure and milk mixture. Co‐digestion of dairy manure and waste milk always produced significantly (P < 0.05) higher total gas and methane gas than digestion of manure alone at both temperatures. Gas production in each case was significantly (P < 0.05) higher in thermophilic digestion than in mesophilic digestion. The results demonstrate that thermophilic co‐digestion of dairy manure and waste milk offers more benefits in terms of the environment and economy.  相似文献   

Propofol-ketamine anesthesia for internal fixation of fractures in racehorses   总被引：2，自引：0，他引：2  

Ohta M  Oku K  Mukai K  Akiyama K  Mizuno Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1433-1436

To assess the clinical usability of propofol-ketamine anesthesia for internal fixation of fractures in racehorses, hemodynamics, blood pH and gases, and vital responses to the continuous intravenous anesthesia in 7 surgical cases were analyzed. The quality of induction with propofol was variable for individual horses. The vital signs reflecting circulation, breath, and anesthetic depth were kept good without any troubles throughout the surgery. Mean time from the end of anesthesia to standing up was prolonged, however recovery from anesthesia was calm and smooth in all cases. Propofol-ketamine anesthesia may be a clinically usable technique for internal fixation of fractures in racehorses, however induction with propofol alone is not recommended.  相似文献   

Effect of aeration in reducing phytotoxicity in anaerobic digestion liquor of swine manure     

Dai HANAJIMA  Kazutaka KURODA  Yasuyuki FUKUMOTO  Tomoko YASUDA  Kazuyoshi SUZUKI  Kiyonori HAGA 《Animal Science Journal》2007,78(4):433-439

Numerous reports have accumulated concerning the quality of solid compost. In contrast, there are few for the residue of anaerobic digestion. In this study, the fertilizer value of anaerobic digestion liquor (ADL) was evaluated, and the effect of aeration on the reduction of the phytotoxicity was examined by a germination assay. Low or high aeration (100 or 400 mL/min) was added to 3 L of ADL obtained from a mixture of pig manure and garbage by using 5‐L jar fermenters under a controlled temperature of 30°C. During the 14‐day aeration period, a high aeration rate improved the germination index (GI) score from 5.7% to nearly 80%, while a low aeration rate did not. Although organic matter decomposition, determined as chemical oxygen demand, did not differ with aeration intensity, remarkable differences were observed in the time course of changes in pH, electric conductivity and ammonium‐nitrogen (NH₄‐N) and total nitrogen (T‐N) content. Among these parameters, the NH₄‐N concentration correlated highly with the GI score (r = 0.986). The primary phytotoxic element in ADL was considered to be NH₄‐N, and the stripping of ammonia (NH₃) by high aeration resulted in the improvement of the GI score. Although the ratio of major nutrients N : P₂O₅ : K₂O (1:0.41:0.94) in ADL was at nearly same level as conventional liquid fertilizer, special attention should be paid to the high concentration of NH₃ when drawing up a fertilization plan.  相似文献   

EXTRA-ABDOMINAL CHONDROLIPOMA IN A DOG     

Shigeyuki Tanabe  Kazutaka Yamada  Yoshiyasu Kobayashi  Koudai Suzuki  Miho Yamaguchi  Yuji Uzuka  Takao Sarashina  Michiyo Inoue 《Veterinary radiology & ultrasound》2005,46(4):306-308

A 10-year-old male maltise dog was presented for evaluation of a gradual abdominal enlargement, which had developed over 2 years. (On the radiograph of the abdomen, a significant distention of the abdomen with a large accumulation of fat could be seen.) The mass effect was noticeable because of the rightward displacement of the viscera. There was multifocal amorphous mineralization, mostly in the right abdomen. Computed tomography (CT) identified a 13 x 13 x 10 cm mass, most likely an intra-abdominal lipoma, by which the digestive tract had been displaced in the abdomen. It was only during surgery, however, that the mass was found to be located between the peritoneum and transversus abdominis muscles and the rectus abdominus muscle. It was completely resected. Histopathological examination revealed that the mass was chondrolipoma. The amorphous radiopaque lesion on the images corresponded to cartilaginous tissues with calcification. There has been no recurrence for 10 months. This is the first report of an extra-abdominal chondrolipoma in dogs. Cartilaginous metaplasia of lipoma, located in the abdominal area, should be included in the differential diagnosis for multifocal amorphous mineralization.  相似文献