Effects of chitin and chitosan on collagen synthesis in wound healing   总被引：7，自引：0，他引：7  

Kojima K  Okamoto Y  Kojima K  Miyatake K  Fujise H  Shigemasa Y  Minami S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1595-1598

Collagen synthesis was evaluated by measuring prolyl hydroxylase (PHL) activity induced within rat granulation tissue by a polyester non-woven fabric (NWF, 1 x 1 cm, 0.6 mm in thickness) impregnated with a chitin or chitosan suspension ranging in concentration from 0.1 to 10 mg/ml. In addition, PHL activity induced in rat granulation tissue by a NWF impregnated with a phosphate buffer solution was examined as a control. The PHL activity in each group remained low until 4 days post-implantation (Day 4). However, in the 10 mg chitin group, the PHL activity increased rapidly without scatter of the data at Day 7 and remained at a plateau until Day 14. In other groups, PHL activity increased linearly until Day 14. The data varied widely at Day 7. Compared to chitosan, chitin at the higher concentration was found to induce stable collagen synthesis in the early wound healing process.  相似文献   

High genetic stability of TM1 and TM2 strains of subtype B feline immunodeficiency virus in long-term infection     

Ikeda Y  Miyazawa T  Nishimura Y  Nakamura K  Tohya Y  Mikami T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(3):287-289

To know the genetic changes of feline immunodeficiency virus (FIV) in long-term infection in cats, we inoculated three specific pathogen-free cats with FIV isolates and determined a partial env sequence covering the V3-V5 region. In 2 cats infected with subtype B strains TM1 and TM2, only one amino acid change in region V3 was observed at 9 years post infection (y.p.i.), and no nucleotide substitutions were observed between 9 and 10 y.p.i., indicating that these strains are genetically stable. On the other hand, in a cat infected with subtype A strain Petaluma at 8.7 y.p.i., 3 nucleotide insertions (one amino acid insertion) in region V5, and 1 synonymous nucleotide substitution and 2 non-synonymous nucleotide substitutions in region V5, were observed.  相似文献   

Tubulopapillary carcinoma with spindle cell metaplasia of the mammary gland in a cat     

Matsuda K  Kobayashi S  Yamashita M  Hirayama K  Kadosawa T  Taniyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(5):479-481

Sarcomatous proliferation of spindle cells was present in the mammary gland and many metastatic sites in a 10-year-old female domestic cat with tubulopapillary carcinoma in the mammary gland. Transition from neoplastic tubular structures to spindle cells in the primary site and fascicular proliferation of the spindle cells with or without coexistance of tubulopapillary carcinoma in the primary and metastatic sites were observed. Most of spindle cells were positive for cytokeratin CAM5.2 as well as the normal luminal epithelium but not the myoepithelium. From these results, this case was diagnosed as tubulopapillary carcinoma with spindle cell metaplasia and it was clarified that neoplastic luminal epithelial cells can transform to sarcomatous appearence.  相似文献   

CHORDOMA OF THE TAIL IN A DEGU (OCTODON DEGUS)     

Hirotaka Kondo  Kazuya Hara  Akihiro Sukegawa  Hisashi Shibuya 《Journal of Exotic Pet Medicine》2018,27(4):1-4

A 6-year-old female degu (Octodon degus) in good body condition was presented with a mass at the base of the tail. Following presentation and the owners’ approval, the tail of the degu was surgically amputated. The mass was submitted for pathological evaluation following the surgical procedure. Histologically, the mass was comprised of lobules and sheets of polygonal cells displaying moderate amounts of basophilic foamy cytoplasm (physaliferous cells). The pathological results of the tail mass were consistent with chordoma. Approximately 3 months following the tail amputation the patient died.  相似文献   

Molecular Demonstration of Hemotropic Mycoplasmas in Wild Japanese Monkeys (Macaca fuscata)     

Hinako SASHIDA  Yoshihisa SUZUKI  Sou ROKUHARA  Kazuya NAGAI  Ry? HARASAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(1):97-101

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Extranodal Lymphoma with Peripheral Nervous System Involvement in a Dog     

Hiroshi UENO  Kenjiro MIYOSHI  Sho FUKUI  Yumi KONDO  Kazuya MATSUDA  Tsuyoshi UCHIDE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(5):723-727

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Production of asexual and sexual offspring in the triploid sexual planarian Dugesia ryukyuensis     

Kazuya KOBAYASHI  Sachiko ARIOKA  Motonori HOSHI  Midori MATSUMOTO 《Integrative zoology》2009,4(3):265-271

Certain freshwater planarians reproduce asexually as well as sexually, and their chromosomal ploidies include polyploidy, aneuploidy and mixoploidy. Previously, we successfully performed an experiment in which a clonal population produced by asexual reproduction of the Dugesia ryukyuensis (OH strain) switched to the sexual mode of reproduction. Worms of this strain are triploid with a pericentric inversion on Chromosome 4. The worms were switched to sexual reproduction after being fed with sexually mature Bdellocephala brunnea, which is a sexually reproducing species. The resulting sexualized OH strain produced cocoons filled with several eggs. Two putative factors, Mendelian factor(s) and chromosomal control(s), have been proposed as determining the reproductive mode. The present study demonstrated that inbreeding of the resultant sexualized worms produced the following four types of offspring through sexual reproduction: diploid asexual worms, triploid asexual worms, diploid sexual worms and triploid sexual worms. The chromosomal mutation on Chromosome 4 was inherited by these offspring independent of their reproductive mode. These results provide two important pieces of information: (i) the putative genetic factor was not necessarily inherited in a Mendelian fashion; and (ii) the reproductive mode is not regulated by chromosomal changes such as polyploidy or chromosomal mutations. This suggests that asexuality in D. ryukyuensis is regulated by an unknown factor(s) other than a Mendelian factor or a chromosomal control.  相似文献   

Contrast harmonic imaging of canine hepatic tumors   总被引：2，自引：0，他引：2  

Kutara K  Asano K  Kito A  Teshima K  Kato Y  Sasaki Y  Edamura K  Shibuya H  Sato T  Hasegawa A  Tanaka S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(5):433-438

Six adult healthy Beagles were used to investigate the hepatic perfusion dynamics of Levovist, a contrast agent used in contrast harmonic imaging (CHI). In addition, 8 dogs with hepatocellular carcinoma (HCC) and 2 dogs with metastatic hepatic hemangiosarcoma (HSA) were used to characterize both the CHI findings with Levovist. In the Beagles, the start of intravenously injected Levovist into the aorta between the cranial mesenteric and renal arteries and the portal vein at the hepatic hilum were 5.47 +/- 1.52 sec and 16.03 +/- 3.39 sec, respectively. As a characteristic CHI finding in the 8 dogs with HCC, the early arterial phase showed a fine network of blood flow enhanced at the surrounding region and within the tumor in all the 8 dogs (100%), and the post vascular phase demonstrated a defect in the whole tumor and an enhancement of the surrounding hepatic tissues in 7 dogs (87.5%). In the 2 dogs with HSA, characteristic finding in which the early arterial and late vascular phases showed a rim contrast enhancement pattern, and the post vascular phase revealed that the whole tumor lacked contrast enhancement and the surrounding hepatic tissues was clearly enhanced. In dogs, the start of the early arterial and late vascular phases, and the characterizations of the CHI findings in HCC and HSA were suggested to be similar to those in humans. Therefore, CHI is thought to be useful for the diagnosis of HCC and metastatic hepatic HSA in dogs as well as in humans.  相似文献   

Application of laser-assisted zona drilling to in vitro fertilization of cryopreserved mouse oocytes with spermatozoa from a subfertile transgenic mouse     

Anzai M  Nishiwaki M  Yanagi M  Nakashima T  Kaneko T  Taguchi Y  Tokoro M  Shin SW  Mitani T  Kato H  Matsumoto K  Nakagata N  Iritani A 《The Journal of reproduction and development》2006,52(5):601-606

Development of assisted reproductive technologies is necessary to obtain fertilized oocytes in a subfertile transgenic mouse strain. Here, we showed the application of laser-assisted drilling of the zona pellucida to in vitro fertilization of cryopreserved mouse oocytes with sperm from subfertile transgenic mice (C57BL/6N-Tg(UCP/FAD2)U8 strain). After cryopreservation by vitrification, the recovery and survival rates of the zona-drilled mouse oocytes were 97% (97/100) and 94% (91/97), respectively. In vitro fertilization of the cryopreserved zona-drilled mouse oocytes with sperm from the subfertile transgenic mice was greatly facilitated (60%, 55/91) compared to that of the cryopreserved zona-intact mouse oocytes (11%, 81/768). In vitro fertilized embryos that developed to the 2-cell stage were again cryopreserved by vitrification, and after warming they were transferred into recipient females. Subsequently, six viable offspring were delivered, and all were confirmed to be transgenic mice. These results indicate that laser-assisted zona drilling of oocytes combined with cryopreservation by vitrification may be a useful approach for large-scale production of in vitro fertilized embryos for managing transgenic mouse strains with reproductive disabilities such as subfertile sperm.  相似文献   

Two species of philometrid nematodes (philometridae) from marine fishes off Japan, including Philometroides branchiostegi sp. n. from Branchiostegus japonicus (malacanthidae)     

Moravec F  Nagasawa K  Nohara K 《Folia parasitologica》2012,59(1):71-78

Gravid females of two species of philometrid nematodes (Philometridae) were collected from marine perciform fishes in Japanese waters, mainly from the southern Sea of Japan. Based on light microscopy and scanning electron microscopy examinations, the previously described but poorly known species Philometra cryptocentri Yamaguti, 1961 is redescribed from specimens recovered from the abdominal cavity of Acanthogobius flavimanus (Temminck et Schlegel), Pterogobius elapoides (Günther) and P. zonoleucus Jordan et Snyder (all Gobiidae) (all new host records); the number (14) and arrangement of cephalic papillae in this species are described for the first time. The new species, Philometroides branchiostegi sp. n. from head tissues of Branchiostegus japonicus (Houttuyn) (Malacanthidae), based on a single specimen, is mainly characterized by the embossment of the entire body except for the cephalic end, presence of four submedian pairs of large cephalic papillae of external circle and two small lateral single papillae of internal circle, pair of large papilla-like caudal projections, the oesophagus with a distinct anterior inflation, by a markedly small body (length about 18 mm) and the larvae 306-465 microm long.  相似文献