Evaluation of several wild tuber-bearingSolanum species for scab resistance     

Kazuyoshi Hosaka  Hiroshi Matsunaga  Keiichi Senda 《American Journal of Potato Research》2000,77(1):41-45

One hundred accessions of 18 wild diploid potato species were screened for resistance to scab through three cycles of selection, from which a total of 322 clones were selected as resistant. The putative ancestral species of the cultivated potatoes (Solanum bukasovii, S. canasense andS. multidissectum) produced resistant clones with significantly higher rates. Thus, these are primary gene sources for conferring scab resistance to cultivated potatoes.  相似文献   

Erratum to: Hydrothermal decomposition of various crystalline celluloses as treated by semi-flow hot-compressed water     

Rosnah?Abdullah  Kazuyoshi?Ueda  Shiro?SakaEmail author 《Journal of Wood Science》2014,60(5):376-376

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Databases for technical aspects of immunohistochemistry: 2021 update     

Takayasu Moroki  Saori Matsuo  Hirofumi Hatakeyama  Seigo Hayashi  Izumi Matsumoto  Satoshi Suzuki  Takashi Kotera  Kazuyoshi Kumagai  Kiyokazu Ozaki 《Journal of toxicologic pathology》2021,34(2):161

With the aim of sharing information about the technical aspects of immunohistochemistry (IHC) and facilitating the selection of suitable antibodies for histopathological examination, this technical report describes the results of a questionnaire distributed during the period of 2018 to 2019 among members of the Conference on Experimental Animal Histopathology. Additionally, it describes the immunological properties and supplier details (clone, supplier, catalog number, species reactivity, etc.) as well as the IHC staining conditions (fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time, incubation temperature, positive control tissue, blocking condition, secondary antibody information, etc.) for a total of 509 primary antibodies (comprising 220 different types). These survey results were an update on the contents reported by CEAH in 2017.  相似文献   

Chloroplast DNA Variation in the Most Primitive Cultivated Diploid Potato Species Solanum stenotomum Juz. et Buk. and its Putative Wild Ancestral Species using High-Resolution Markers   总被引：1，自引：0，他引：1  

Thitaporn Sukhotu  Osamu Kamijima  Kazuyoshi Hosaka 《Genetic Resources and Crop Evolution》2006,53(1):53-63

Solanum stenotomum Juz. et Buk. (2n = 2x = 24) is considered to be the most primitive diploid cultivated species from which all the other Andean cultivated potatoes were originated (Hawkes 1990). To disclose chloroplast DNA (ctDNA) variability and the maternal origin of S. stenotomum, 36 accessions of S. stenotomum and 86 accessions of putative wild ancestral species were determined for ctDNA types and analyzed by high-resolution markers (seven ctDNA microsatellites and an H3 marker). High-resolution markers discriminated 57 different ctDNAs (haplotypes), which were classified into the W-type ctDNA group and C-, S- and A-type ctDNA group, and within the latter group S- and A-type ctDNAs were distinct from each other among many different haplotypes mostly having C-type ctDNA. This ctDNA relationship supported our previous findings obtained for mostly Andean cultivated species (Sukhotu et al. 2004). Compared with other putative ancestral wild species, S. stenotomum showed somewhat limited ctDNA diversity, having two major haplotypes 1 and 2 also found in different wild species in different places. Therefore, the ctDNA in S. stenotomum was of at least dual origins either by successive domestication from different species or else by introgression after initial S. stenotomum arose.  相似文献   

Net blotch resistance in wild species of Hordeum     

Kazuhiro Sato  Kazuyoshi Takeda 《Euphytica》1997,95(2):179-185

Resistance to net blotch was evaluated in 175 Hordeum vulgare subsp. spontaneum (H. spontaneum) accessions and 149 accessions of thirteen species or subspecies of wild Hordeum. Most H. spontaneum accessions showed resistance to each of the four Pyrenophora teres f. teres (P. teres) isolates tested. However, H. spontaneum accessions showed different resistance reactions, depending upon their origin. In particular, some accessions from Afghanistan and Russia showed a high level of resistance, and accessions from Morocco were susceptible. Among the four P. teres isolates, the virulence spectra on the H. spontaneum accessions were more different between isolates from different countries than between those from the same country. Hordeum spontaneum accessions susceptible to the Canadian isolate WRS102, but resistant to the other three isolates were found in Iraq suggesting the geographical differentiation of resistance genes in H. spontaneum. All accessions of the other wild Hordeum species, especially some accessions of H. marinum subsp. gussoneanum, showed high levels of resistance. These resistance genes may be useful candidates for incorporation into cultivated barley. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Artificial incubation of Japanese crayfish (Cambaroides japonicus) eggs by using a simple, easy method with a microplate     

Kazuyoshi Nakata  Hajime Matsubara  Seiji Goshima 《Aquaculture (Amsterdam, Netherlands)》2004,230(1-4):273-279

We developed a simple, easy method with a microplate to artificially incubate Japanese crayfish (Cambaroides japonicus) eggs for their cultivation. We prepared 6-, 12-, 24-, 48-, and 96-well microplates containing sterile water heated to 5, 10, 15, and 20 °C. Fourteen experimental groups for each water temperature were prepared for each of different water volumes (0.125–10 ml) in each well. One embryonic egg was placed in each well. Experiments were also conducted with water collected from the lake where the eggs were harvested from and held at 15 °C. In the microplates with sterile water, high proportions of eggs hatched (60–100%) at 15 °C in all volumes of water, although the proportions of hatching were low (0–20%) at 5, 10, and 20 °C. All eggs died in the experiments that used lake water. We conclude that the 96-well would be the most effective size to hatch crayfish eggs in, because of its convenience. This method using a microplate is simpler and easier compared with methods of previous studies to artificially incubate crayfish eggs, and therefore it might be useful to incubate eggs of other freshwater crayfish species.  相似文献   

Timing of egg hatch of amphidromous freshwater shrimps in a small river (the Nishida River), western Japan     

Keiko Ideguchi  Tatsuo Hamano  Kazuyoshi Nakata 《Fisheries Science》2007,73(4):961-963

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Chromosome arm location of the gene controlling leaf pubescence of a Chinese local wheat cultivar ‘Hong-mang-mai’     

Shin Taketa  C.L. Chang  Makoto Ishii  Kazuyoshi Takeda 《Euphytica》2002,125(2):141-147

The inheritance of the leaf pubescence character of a Chinese local wheat cultivar ‘Hon-mang-mai’ was investigated by monosomic and telosomic analyses. Leaf pubescence was evaluated by observation of the adaxial side of the penultimate leaf of adult plants. F₁ hybrids of ‘Hong-mang-mai’ with a non-pubescent cultivar ‘Chinese Spring’ had leaf pubescence, but its density was about a half of that of ‘Hong-mang-mai’. In the F₂ generation, the segregation ratio of pubescent to non-pubescent plants fitted a ratio of 3: 1, suggesting that leaf pubescence was controlled by one dominant gene. Monosomic analysis revealed that the gene for pubescence is located on chromosome 7B. Telosomic analysis showed that the gene is located on the short arm of chromosome 7B with a distance of 14.3%from the centromere. This gene is not allelic with the previously reported hairy leaf gene Hl on chromosome 4B, and therefore, is designated Hl2, hairy leaf 2. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

A surge-like increase in luteinizing hormone preceding musth in a captive bull African elephant (Loxodonta africana)     

Kaewmanee S  Watanabe G  Keio M  Yamamoto Y  Yamamoto T  Kishimoto M  Nagaoka K  Narushima E  Katayanagi M  Nakao R  Sakurai Y  Morikubo S  Kaneko M  Yoshihara M  Yabe T  Taya K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(3):379-383

This study was conducted to determine the correlation between reproductive hormones and musth in a male African elephant. Changes in circulating luteinizing hormone (LH), follicle stimulating hormone (FSH), testosterone and immunoreactive (ir-) inhibin and the degree of musth were evaluated for 4 years. LH increased 4 weeks before musth began. The highest concentrations of testosterone and ir-inhibin were observed from April to October. There were positive correlations among testosterone, ir-inhibin and musth behavior. These findings suggested that the surge-like LH in the pre-musth period might stimulate secretion of testosterone and ir-inhibin and thus initiate the musth behavior. This study also suggested that the high LH level before musth might be a useful biomarker for the beginning of the musth season.  相似文献   

Effects of growth factors on development of fetal islet B-cells in vitro   总被引：1，自引：0，他引：1  

Yasuda M  Yamamoto M  Ochiai H  Eguchi Y  Arishima K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(8):807-811

To investigate the role of growth factors (epidermal growth factor [EGF], betacellulin, and activin A) in the development of islet B cells of rat fetal pancreatic explants in vitro, pancreases from rat fetuses at day 18 of gestation were cultured for 96 hr, with or without these growth factors. Culture medium was changed every 24 hr, and the level of insulin released in the culture medium was measured. After 72 hr of culture, pancreases were examined histologically. As a result, EGF promoted cell proliferation, but reduced B cell volume. Whereas, betacellulin and activin A inhibited cell division, but promoted increased B cell volume and insulin secretion, especially activin A, which stimulated insulin release in a time dependent manner. These results suggest that EGF, betacellulin, and activin A promote pancreatic cell proliferation, islet B-cell differentiation, and islet B-cell differentiation and functional maturation, respectively, and that EGF, betacellulin, and activin A, in this order, regulate islet B-cell neogenesis.  相似文献