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排序方式: 共有325条查询结果,搜索用时 15 毫秒
271.
272.
Keisuke Yamano Atushi Fujiwara Akifumi Nakamura Michiyasu Yoshikuni 《Fisheries Science》2013,79(5):823-832
We describe the process of cubifrin-induced in vitro oocyte maturation in the Japanese sea cucumber Apostichopus japonicus, and we propose a procedure for practical hatchery application. Oocyte maturation occurred 40–50 min after stimulation of the ovaries by cubifrin. Concurrently, eggs were released from the ovarian tubules, initially by slow peristalsis, followed by active release after about 60 min. The first and second polar bodies were released from the eggs at 90–100 min and about 140 min, respectively. Cubifrin was more effective in inducing oocyte maturation at 1–100 nM than at 100 pM. Cubifrin at 1 pM–10 nM in rearing seawater had no effect on larval development. Delayed fertilization reduced the developmental ability of eggs. Broodstock could be maintained at 15–16 °C for up to about 50 days without affecting the developmental ability of eggs. Eggs matured in vitro and in vivo were similar in their developmental ability. In conclusion, the in vitro induction of oocyte maturation is effective in Japanese sea cucumber and represents a viable alternative to the induction of spawning by cubifrin injection. 相似文献
273.
Takumi YOSHIDA Md Emtiaj ALAM Keisuke HANAFUSA Yasunori TSUJIMOTO Masaya TSUKAMOTO Ryoji KANEGI Toshio INABA Kikuya SUGIURA Shingo HATOYA 《The Journal of reproduction and development》2022,68(2):160
We examined the effectiveness of saline, Euro-Collins solution (EC), and ET-Kyoto solution (ET-K) as preservation media for the cold storage of feline ovaries. Ovaries were maintained in these media at 4°C for 24, 48, or 72 h until oocyte retrieval. The ET-K group exhibited a higher oocyte maturation rate than the saline group after 72 h of storage. Moreover, ET-K could sustain the competence of the feline oocytes to cleave after 48 h, and the morula formation rate of the ET-K group was higher than that of the other groups after 24 and 48 h. Furthermore, the ET-K group exhibited a higher blastocyst formation rate than the other groups after storage for 24 h, and only ET-K retained the developmental competence in blastocysts after 48 h of storage. In addition, regarding the cell numbers of the blastocysts, there was no significant difference among the tested groups. In conclusion, our results indicate that ET-K is a suitable preservation medium for feline ovaries. 相似文献
274.
Kumiko NAGAYAMA Keisuke OGUMA Hiroshi SENTSUI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1531-1534
Bovine viral diarrhea virus (BVDV) isolation and antibody survey were performed using
2,758 fetal bovine sera (FBS) collected from slaughterhouses in New Zealand, Australia and
the Dominican Republic, and then sent to Japan to manufacture commercial serum for cell
culture use. FBS in the Dominican Republic were pooled for each several individuals, and
those collected in other countries were separated according to each individual and
subjected to the tests. BVDV was isolated from 25 (0.91%) FBS, and the BVDV antibody was
detected in 44 (1.60%) FBS. The survey on 139 sets of paired sera of a dam and her fetus
revealed that neither the BVDV antibody nor BVDV was detected in all FBS from BVDV
antibody-positive dams. 相似文献
275.
Hakimullah HAKIM Chanathip THAMMAKARN Atsushi SUGURO Yuki ISHIDA Akinobu KAWAMURA Miho TAMURA Keisuke SATOH Misato TSUJIMURA Tomomi HASEGAWA Kazuaki TAKEHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):211-215
Hypochlorous acid (HOCl) solutions were evaluated for their virucidal ability against a low pathogenic avian influenza virus (AIV), H7N1. HOCl solutions containing 50, 100 and 200 ppm chlorine (pH 6) or their sprayed solutions (harvested in dishes placed at 1 or 30 cm distance between the spray nozzle and dish) were mixed with the virus with or without organic materials (5% fetal bovine serum: FBS). Under plain diluent conditions (without FBS), harvested solutions of HOCl after spraying could decrease the AIV titer by more than 1,000 times, to an undetectable level (< 2.5 log10TCID50/ml) within 5 sec, with the exception of the 50 ppm solution harvested after spraying at the distance of 30 cm. Under the dirty conditions (in the presence of 5% FBS), they lost their virucidal activity. When HOCl solutions were sprayed directly on the virus on rayon sheets for 10 sec, the solutions of 100 and 200 ppm could inactivate AIV immediately after spraying,
while 50 ppm solution required at least 3 min of contact time. In the indirect spray form, after 10 sec of spraying, the lids of the dishes were opened to expose the virus on rayon sheets to HOCl. In this form, the 200 ppm solution inactivated AIV within 10 min of contact, while 50 and 100 ppm could not inactivate it. These data suggest that HOCl can be used in spray form to inactivate AIV at the farm level. 相似文献
276.
Makoto Sato Setsuko Todoriki Tetsuyuki Takahashi Ezar Hafez Chie Takasu Hisanori Uehara Kohji Yamakage Takashi Kondo Kozo Matsumoto Masakazu Furuta Keisuke Izumi 《Journal of toxicologic pathology》2015,28(2):99-107
A 90-day oral toxicity test in rats was performed to evaluate the toxicity of
2-tetradecylcyclobutanone (2-tDCB), a unique radiolytic product of stearic acid.
Six-week-old male and female F344 rats (n=15/group) were given 2-tDCB at concentrations of
0, 12, 60 and 300 ppm in a powder diet for 13 weeks. Slight dose-dependent increases in
serum total protein and albumin in male rats were found, but these changes were not
considered to be a toxic effect. The fasting, but not non-fasting, blood glucose levels of
the male rats in the 300 ppm group and female rats in the 60 and 300 ppm groups were lower
than those of the controls. Gas chromatography-mass spectrometry analysis showed
dose-dependent accumulation of 2-tDCB in adipose tissue, notably in males. Next, we
performed an azoxymethane (AOM)-induced two-stage carcinogenesis study. After injection of
6-week-old male F344 rats (n=30/group) once a week for 3 weeks, the animals received
2-tDCB at concentrations of 0, 10, 50 and 250 ppm in a powder diet for 25 weeks. The
incidences of colon tumors for the 2-tDCB dosages were 34%, 45%, 40% and 37%,
respectively, and were not statistically significant. These data suggest that 2-tDCB shows
no toxic or tumor-modifying effects under the present conditions, and that the
no-observed-adverse-effect level for 2-tDCB is 300 ppm in both sexes, equivalent to 15.5
mg/kg b.w./day in males and 16.5 mg/kg b.w./day in females. 相似文献
277.
Mitsutoshi Abe Tetsuhiko Yoshimura Naoki Yasukawa Keisuke Koba Kazuyuki Moriya Tetsuro Sakai 《Journal of Forest Research》2005,10(1):43-50
Field education programs have been conducted throughout the world because learning through experience is an important process in environmental and forest education. We developed a support system for forest education that consists mainly of a Global Positioning System (GPS) and personal digital assistant (PDA). Moreover, field experiments were conducted to evaluate educational efficacy as well as usability of the developed system. Through a post-questionnaire the developed system was highly evaluated in terms of educational efficacy and enjoyment. On the other hand, the questionnaire revealed the need for technical improvements. For example, the drawing function, which involves the use of a stylus pen, was the most poorly evaluated because a drawing could not be partially erased as with an eraser and representation was not sufficiently accurate. In addition, positions where information on nature appeared were sometimes inaccurate due to positional errors caused by using the autonomous GPS. Some participants answered that walking with the developed system was also troublesome due to its shape and size. In fact, they did not feel comfortable about the cables connecting the GPS to the PDA and GPS to its antenna. Although there were some problems with the system, we believe that the field education program was successful because 95% of the participants answered that they would join a similar program again. 相似文献
278.
Hair growth is a highly regulated cyclical process. Three distinct phases have been defined for the mammalian cycle: anagen (growing phase), catagen (regression phase), and telogen (resting phase). Although little is known about the mechanism that regulates the hair cycle, it is believed that dermal papillae (DP) derived from mesenchymal cells play an essential role in controlling the established hair follicle and hair cycle. The purpose of this investigation was to find the components of woods and fungi that exert a proliferative activity on DP cells. Results show that the fungus YL161 (ethyl acetate extract, 1ppm),Agaricus blazei (ethyl acetate extract, 0.1 ppm), and the bark ofCamptotheca cuminata (methanol extract of bark, 0.1 ppm) exhibit higher growth-promoting activity than pentadecanoic acid. These components that have a proliferative effect on DP cells may be useful hair growth-stimulating materials and can be used to understand the mechanism of hair growth. 相似文献
279.
Mitsutoshi Abe Tetsuhiko Yoshimura Satoshi Koizumi Naoto Hasegawa Tomohiro Osaki Naoki Yasukawa Keisuke Koba Kazuyuki Moriya Tetsuro Sakai 《Journal of Forest Research》2005,10(3):189-197
Forest education is generally conducted in a real forest to provide learners a direct experience with reality. However, often it is not easy to participate in forest education programs carried out in remote places due to distance, limited time, or economic or physical restrictions. Therefore, we developed a walk-through system for forest education in a virtual forest and evaluated its educational efficacy as well as usability through indoor experiments. The virtual forest was constructed with omnidirectional images taken in a forest using a digital camera with an omnidirectional vision sensor. The results of the postquestionnaire showed that the developed system was highly evaluated by the participants in the experiments in terms of educational efficacy and enjoyment. Also, comparison of results of prequestionnaire and postquestionnaire showed that it had educational value. On the other hand, some technical problems in the developed system were revealed. For example, 45.0% of the participants answered that the walk-through in the virtual forest did not proceed with sufficient smoothness, and encountered unexpected slowdowns and stops. In addition, image quality was not high enough to identify small objects in the virtual forest. Regardless of these problems, we believe that the computer-based forest education was successful because 88.3% of the participants answered that they would like to join a similar program again and 90.0% answered that they would like to visit the real forest that the virtual forest imitated. 相似文献
280.
Keisuke Tomioka Yuuri Hirooka Akane Takezaki Takayuki Aoki Toyozo Sato 《Journal of General Plant Pathology》2011,77(2):132-135
Rotting of roots and stem bases and wilting of entire plants were found on a gentianaceous flowering plant, prairie gentian
(Eustoma grandiflorum), grown in Kagawa Prefecture in the southwest region of Japan in April 2001. A mitosporic fungus, isolated repeatedly from
the diseased plants, was identified as a species belonging to the clade 3 of Fusarium solani species complex based on the morphology and the sequence of the translation elongation factor gene. It was demonstrated to
cause the disease by inoculating potted plants and reisolating the fungus from the diseased plants. We propose the name “Fusarium
root rot of prairie gentian” for this disease. 相似文献