Antimicrobial susceptibility of Campylobacter jejuni, Campylobacter coli, and Campylobacter laridis from cats, dogs, pigs, and seagulls     

C Kaneuchi  M Ashihara  Y Sugiyama  T Imaizumi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(3):685-691

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Reliability of the dye and modified hemagglutination tests for the latent infection of toxoplasma     

Y Katsube  T Hagiwara  K Imaizumi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1972,34(3):123-133

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Pathogenicity of Sendai virus in mice cage-mated with infectors and their offsprings     

M Nakagawa  M Saito  K Kinoshita  E Suzuki  K Imaizumi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1980,42(3):337-344

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Discovery of single-nucleotide mutations in acetolactate synthase genes by Ecotilling     

Guang-Xi Wang  Mui-Keng Tan  Hiromasa Saitoh  Toshiyuki Imaizumi  Tohru Tominaga 《Pesticide biochemistry and physiology》2007,88(2):143-148

TILLING (Targeting Induced Local Lesions IN Genomes) is a powerful reverse genetic technique that employs a mismatch-specific endonuclease to discover induced point mutations in genes of interest. The use of the TILLING technique to survey natural variation in genes is called Ecotilling. We report an adaptation of Ecotilling for rapid detection of single-nucleotide mutations in the acetolactate synthase (ALS) genes of sulfonylurea (SU)-resistant (R) Monochoria vaginalis (Pontederiaceae), a paddy weed, in Japan. Genomic DNA of a SU-R plant (target DNA) was mixed with the DNA of a SU-susceptible (S) plant (reference DNA). Ecotilling detected two nucleotide mutations in the ALS gene of SU-R M. vaginalis. These 2 mutations were confirmed by DNA sequencing. A single nucleotide mutation (C to A), in the codon CCT to CAT and another mutation (C to T), in the codon CCT to TCT were identified by sequencing. Both mutations result in the disruption of a Pro codon in the conserved Domain A region with the consequent substitution of a His residue in the first mutation and a Ser residue in the second. Substitution of the Pro residue in Domain A of the ALS gene has been reported to result in insensitivity to SUs in many weed biotypes. This study demonstrates that Ecotilling is a fast, reliable, economical method for detecting single-nucleotide mutations in genes arising from herbicide selection.  相似文献   

Gibberella ear rot of corn caused by <Emphasis Type="Italic">Fusarium asiaticum</Emphasis> in Japan     

Akira?KawakamiEmail author  Naoki?Kato  Takahide?Sasaya  Keisuke?Tomioka  Hiroyoshi?Inoue  Atsushi?Miyasaka  Kazuyuki?Hirayae 《Journal of General Plant Pathology》2015,81(4):324-327

Ear rot with white or pink mold was found on corn (Zea mays L.). A species of Fusarium, not registered previously as a pathogen causing Gibberella ear rot of corn in Japan, was isolated from the rotted ear. The isolates, identified as F. asiaticum based on morphological characteristics and nucleotide sequences, caused white or pink mold on corn ear after inoculation. Moreover, the 3-acetyl deoxynivalenol chemotype and the nivalenol chemotype were found in the isolates. We propose to include F. asiaticum as one of the pathogens causing Gibberella ear rot of corn in Japan.  相似文献   

Comparison of the behavior of skipjack (Katsuwonus pelamis), yellowfin (Thunnus albacares) and bigeye (T. obesus) tuna associated with drifting FADs in the equatorial central Pacific Ocean          下载免费PDF全文

Takayuki Matsumoto  Keisuke Satoh  Yasuko Semba  Mikio Toyonaga 《Fisheries Oceanography》2016,25(6):565-581

We evaluated the behavior of skipjack (Katsuwonus pelamis), yellowfin (Thunnus albacares) and bigeye tuna (T. obesus) associated with drifting fish aggregating devices (FADs) in the equatorial central Pacific Ocean. A total of 30 skipjack [34.5–65.0 cm in fork length (FL)], 43 yellowfin (31.6–93.5 cm FL) and 32 bigeye tuna (33.5–85.5 cm FL) were tagged with coded transmitters and released near two drifting FADs. At one of the two FADs, we successfully monitored the behavior of all three species simultaneously. Several individuals remained around the same FAD for 10 or more days. Occasional excursions from the FAD were observed for all three species, some of which occurred concurrently for multiple individuals. The detection rate was higher during the daytime than the nighttime for all the species, and the detection rate for bigeye tuna was higher than for yellowfin or skipjack tuna. The swimming depth was deeper during the daytime than nighttime for all species. The fish usually remained shallower than 100 m, but occasionally dived to around 150 m or deeper, most often for bigeye and yellowfin tuna during the daytime. The swimming depth for skipjack tuna was shallower than that for bigeye and yellowfin tuna, although the difference was not large, and is probably not sufficient to allow the selective harvest of skipjack and yellowfin tuna by the purse seine fishery. From the detection rate of the signals, bigeye tuna is considered to be more vulnerable to the FAD sets than yellowfin and skipjack tuna.  相似文献   

Immobilization of Antimony(III) in Oxic Soil Using Combined Application of Hydroxyapatite and Ferrihydrite     

Shouhei Ogawa  Masahiko Katoh  Chiya Numako  Keisuke Kitahara  Serika Miyazaki  Takeshi Sato 《Water, air, and soil pollution》2016,227(4):124

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Biochemical and genomic analysis of neoculin compared to monocot mannose-binding lectins     

Shimizu-Ibuka A  Nakai Y  Nakamori K  Morita Y  Nakajima K  Kadota K  Watanabe H  Okubo S  Terada T  Asakura T  Misaka T  Abe K 《Journal of agricultural and food chemistry》2008,56(13):5338-5344

Neoculin occurring in an edible tropical fruit is a heterodimeric protein which has both sweetness and a taste-modifying activity that converts sourness to sweetness. Both the primary and the overall tertiary structures of neoculin resemble those of monocot mannose-binding lectins. This study investigated differences in biochemical properties between neoculin and the lectins. Structural comparison between the mannose-binding sites of lectins and the corresponding regions of neoculin showed that there is at least one amino acid substitution at each site in neoculin, suggesting a reason for the lack of its mannose-binding ability. This was consistent with hemagglutination assay data demonstrating that neoculin had no detectable agglutinin activity. DNA microarray analysis indicated that neoculin had no significant influence on gene expression in Caco-2 cell, whereas kidney bean lectin (Phaseolus vulgaris agglutinin) greatly influenced various gene expressions. These data strongly suggest that neoculin has no lectin-like properties, encouraging its practical use in the food industry.  相似文献   

Radiation use efficiency,N accumulation and biomass production of high-yielding rice in aerobic culture   总被引：1，自引：0，他引：1  

Keisuke Katsura  Midori Okami  Hiroaki Mizunuma  Yoichiro Kato 《Field Crops Research》2010

The concept of aerobic culture is to save water resource while maintaining high productivity in irrigated rice ecosystem. This study compared nitrogen (N) accumulation and radiation use efficiency (RUE) in the biomass production of rice crops in aerobic and flooded cultures. The total water input was 800–1300 mm and 1500–3500 mm in aerobic culture and flooded culture, respectively, and four high-yielding rice cultivars were grown with a high rate of N application (180 kg N ha⁻¹) at two sites (Tokyo and Osaka) in Japan in 2007 and 2008. The aboveground biomass and N accumulation at maturity were significantly higher in aerobic culture (17.2–18.5 t ha⁻¹ and 194–233  kg N ha⁻¹, respectively) than in flooded culture (14.7–15.8 t ha⁻¹ and 142–173 kg N ha⁻¹) except in Tokyo in 2007, where the surface soil moisture content frequently declined. The crop maintained higher N uptake in aerobic culture than in flooded culture, because in aerobic culture there was a higher N accumulation rate in the reproductive stage. RUE in aerobic culture was comparable to, or higher than, that in flooded culture (1.27–1.50 g MJ⁻¹ vs. 1.20–1.37 g MJ⁻¹), except in Tokyo in 2007 (1.30 g MJ⁻¹ vs. 1.37 g MJ⁻¹). These results suggest that higher biomass production in aerobic culture was attributable to greater N accumulation, leading to higher N concentration (N%) than in flooded culture. Cultivar differences in response to water regimes were thought to reflect differences in mainly (1) early vigor and RUE under temporary declines in soil moisture in aerobic culture and (2) the ability to maintain high N% in flooded culture.  相似文献   

Isoprenoid-substituted flavonoids from wood of Artocarpus heterophyllus on B16 melanoma cells: Cytotoxicity and structural criteria     

Enos Tangke Arung  Keisuke Yoshikawa  Kuniyoshi Shimizu  Ryuichiro Kondo 《Fitoterapia》2010

As a result of cytotoxicity-guided fractionation, nine flavonoids, artocarpin (1), cudraflavone C (2), 6-prenylapigenin (3), kuwanon C (4), norartocarpin (5), albanin A (6), cudraflavone B (7), brosimone I (8) and artocarpanone (9) were identified from the methanol extract of the wood of Artocarpus heterophyllus, known commonly as Nangka in Indonesia. A structure–activity investigation of the effect of these isolated compounds (1–9) and structurally related compounds on B16 melanoma cells indicated that isoprenoid moiety substitutions in flavonoids enhance their cytotoxicity, and that the position of attachment and the number of isoprenoid-substituent moieties per molecule influence flavonoid cytotoxicity.  相似文献