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排序方式: 共有325条查询结果,搜索用时 15 毫秒
31.
Tsunehisa Miki Hiroyuki Sugimoto Keisuke Kojiro Yuzo Furuta Kozo Kanayama 《Journal of Wood Science》2012,58(4):300-308
The thermal properties and transitions of solid and ground wood samples conditioned at different humidity conditions were investigated by temperature-modulated differential scanning calorimetry. A time-dependent transition was detected as an endothermic peak in the total and non-reversing heat flows and as a step change in the reversing heat flow during the first heating run of samples with moisture contents above 5?%, but it disappeared in the second heating run. These different thermal behaviors indicate that the effect of heat and moisture on the thermal properties of wood is history dependent. This step change in the reversing heat flow is considered to be a glass transition of moist wood. Other relaxation processes (e.g., enthalpy relaxation) occur simultaneously with this glass transition. The temperature ranges of the transition and the relaxation decreased drastically as the moisture content increased up to 11?%, while they remained almost constant at higher moisture contents. In addition, the transitions of the ground wood occurred at lower temperatures than those of the solid wood at similar moisture contents. Kissinger plots revealed that the apparent activation energy for the glass transition of the solid wood with a moisture content of 11?% was about 600?kJ/mol, whereas that of the ground wood was 220?kJ/mol. 相似文献
32.
Shotaro Takeuchi Minoru Saito Keisuke Imaizumi Toshio Kaidoh Hiroki Higuchi Sachiko Inubushi 《Veterinary microbiology》2002,84(1-2):135-142
The gene (aur) encoding the metalloprotease (aureolysin) of Staphylococcus aureus from domestic animals was analyzed by polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism (PCR-RFLP) and sequencing. The aur gene was detected in all 74 isolates from cows, pigs and chickens by PCR amplification and was classified into types I and II by PCR-RFLP patterns. The type II aur gene was contained in 36 (94.7%) of 38 protease-positive isolates as judged by skim milk agar plate culture, while type I was contained in 28 (77.8%) of 36 protease-negative isolates. The deduced amino acid sequences of aureolysins from type I and II isolates were almost identical with those of the published data. Subsequently, the two aureolysins were purified from the culture supernatants of type I and II isolates. The molecular weights of purified type I and II aureolysins were both estimated at 34kDa by SDS-polyacrylamide gel electrophoresis. These aureolysins had maximum proteolytic activity at 30-50 degrees C and pH 7.0-8.0. Their activity was inhibited by metal- and zinc-specific inhibitors, such as EDTA, EGTA and 1,10-phenanthroline. Specific activity (activity/protein) of type II aureolysin was two times higher than that of type I. These results indicated that the aur gene is highly conserved with two allelic forms (types I and II) among bovine, porcine and avian isolates of S. aureus. 相似文献
33.
The effects of laser incising on the surface of heat-affected zones (HAZs) and liquid uptake of wood were examined. Deep pin holes were incised on the tangential section of the wood with a laser beam of 1 kW in power with varied pulse widths, and the HAZs of the holes were investigated using scanning electron microscopy. The length of liquid uptake passing through HAZs surfaces was then measured. It was observed that the HAZs were unable to maintain normal porous structure, and smooth surfaces such as that of wood charcoal were also not observed. This is because the composite of the middle lamella completely melted, which led to a decrease in the length of liquid uptake. In addition, the release of high gas-pressure and thermal energy during the laser-incising process did not damage the tylose and the aspirated pit in the longitudinal direction. Because of this, the liquid movement remained blocked. However, the incising holes provided additional liquid-intake points, resulting in greater intake capacity. Moreover, it was observed that improvement of liquid impregnation is related to hole diameter. 相似文献
34.
Itaru Yamaguchi Kensuke Myojo Hiroko Sanada Eri Sudo Sayaka Ootsuka Hiroshi Okumura Atsuko Takami Tomomi Yoneshige Yui Suzuki Minami Imaizumi Chie Takada Naoya Kimoto Koji Saeki Katsumi Takaba 《Journal of toxicologic pathology》2013,26(3):301-307
We histopathologically and immunohistochemically investigated a case of malignant
lymphoma that spontaneously developed in a male common marmoset at two years of age.
Beginning at two years four months of age, the animal had an enlargement of the
submandibular and inguinal lymph nodes, small subcutaneous nodules near the right breast
and an approximately fivefold increase in peripheral lymphocyte count compared with the
previous examination value. The postmortem findings at two years eight months of age
showed lymphadenopathy with enlargement of the thymus and spleen. Small- to
intermediate-sized neoplastic lymphocytes had diffusely proliferated in the enlarged
nodes. The neoplastic cells were pleomorphic and had irregularly shaped nuclei. The
nuclear chromatin staining revealed hyperchromatism in the small-sized cells, and the
intermediate-sized cells exhibited vesicular staining. An immunohistochemical examination
indicated that the neoplastic lymphocytes were positive for CD3 and negative for CD20,
thus suggesting that they had originated from T cells. In addition, the proliferation of
high endothelial venules and reactive epithelioid histiocytes was observed. Scattered
tingible body-laden macrophages were infrequently detected. Neoplastic lymphocytes were
also observed in the thymus, spleen, heart, lungs, liver, kidneys, adrenal glands and
femoral and sternal bone marrow. This malignant lymphoma in a young male common marmoset
was considered to fit the category of “peripheral T-cell lymphoma, not otherwise specified
(PTCL-NOS)” according to the new WHO system of classification. 相似文献
35.
Duangjai Pisuttharachai Motoshige Yasuike Hideaki Aono Keisuke Murakami Hidehiro Kondo Takashi Aoki Ikuo Hirono 《Fisheries Science》2009,75(1):195-206
Two complementary DNA (cDNA) libraries were constructed from phyllosomas and hemocytes of adult Japanese spiny lobster Panulirus japonicus and a total of 2,673 expressed sequence tags (ESTs) were obtained. After assembly and clustering, 450 and 458 unique sequences
were found from the phyllosoma and hemocyte cDNA libraries, respectively. Of these, 114 and 220 ESTs showed significant homologies
with known genes in the National Centre for Biotechnology Information (NCBI) database. The remaining sequences were of unknown
function. Immune-related genes found in this study include lectin, proteinase inhibitor, prophenoloxidase, heat-shock protein,
antimicrobial peptide, and a few putative defense-related proteins. 相似文献
36.
37.
Tamaki Masahiko Kobayashi Fumiyuki Suehiro Keisuke Ohsato Shuichi Sato Michio 《农业科学学报》2018,17(9):2024-2030
We investigated the possibility to reduce the usage of Blasin®Flowable (BF), a disinfectant inhibiting the germination and appressorium formation of Pyricularia oryzae Cavara conidia, by using carbon dioxide microbubbles (CO2MB). Germination was significantly inhibited by 10 000-fold diluted BF solution containing CO2MB generated by the decompression-type generator compared to CO2 millibubbles (CO2MMB) and CO2MB generated by the gas-water circulating-type generator. Appressorium formation in the 10 000-fold diluted BF solution containing both CO2MBs was less than that in CO2MMB. Scanning electron microscopy showed wrinkles and dents on the surface of conidia treated with 5 000-fold diluted BF solution containing both CO2MBs. Via transmission electron microscopy, we observed the expansion of the vacuole and the intracellular space and bloated or absent lipid granules in the conidia treated with BF solution containing both CO2MBs. Our results show that inhibition of the conidium germination and appressorium formation of P. oryzae Cavara by 10 000-fold diluted BF solution could be achieved by using the decompression-type CO2MB. 相似文献
38.
Yohei Kawasaki Yu Tanaka Keisuke Katsura Larry C. Purcell Tatsuhiko Shiraiwa 《Plant Production Science》2016,19(2):257-266
The difference in yields of cultivars may be causing difference in soybean yield between Japan and the USA. The objective of this study was to identify the effect of the cultivar on dry matter production and to reveal the key factors causing the differences in yield by focusing utilization of solar radiation in recent Japanese and US soybean cultivars. Field experiments were conducted during two seasons in Takatsuki, Japan (34°50′), and in a single season in Fayetteville (36°04′), AR, USA. Five Japanese and 10 US cultivars were observed under near-optimal conditions in order to achieve yields as close to their physiological potential as possible. The seed yield and total aboveground dry matter (TDM) were measured at maturity as long as radiation was intercepted by the canopy. The seed yield ranged from 3.10t ha?1 to 5.91t ha?1. Throughout the three environments, the seed yield of US cultivars was significantly higher than that of Japanese cultivars. The seed yield correlated with the TDM rather than the HI with correlation coefficients from .519 to .928 for the TDM vs. .175 to .800 for the HI, for each of the three environments. The higher TDM of US cultivars was caused by a higher radiation use efficiency rather than higher total intercepted radiation throughout the three environments. The seasonal change in the TDM observed in four cultivars indicated that dry matter productivity was different between cultivars, specifically during the seed-filling period. 相似文献
39.
40.
Imtiaj Hasan Shigeki Sugawara Yuki Fujii Yasuhiro Koide Daiki Terada Naoya Iimura Toshiyuki Fujiwara Keisuke G. Takahashi Nobuhiko Kojima Sultana Rajia Sarkar M. A. Kawsar Robert A. Kanaly Hideho Uchiyama Masahiro Hosono Yukiko Ogawa Hideaki Fujita Jiharu Hamako Taei Matsui Yasuhiro Ozeki 《Marine drugs》2015,13(12):7377-7389
MytiLec; a novel lectin isolated from the Mediterranean mussel (Mytilus galloprovincialis); shows strong binding affinity to globotriose (Gb3: Galα1-4Galβ1-4Glc). MytiLec revealed β-trefoil folding as also found in the ricin B-subunit type (R-type) lectin family, although the amino acid sequences were quite different. Classification of R-type lectin family members therefore needs to be based on conformation as well as on primary structure. MytiLec specifically killed Burkitt''s lymphoma Ramos cells, which express Gb3. Fluorescein-labeling assay revealed that MytiLec was incorporated inside the cells. MytiLec treatment of Ramos cells resulted in activation of both classical MAPK/ extracellular signal-regulated kinase and extracellular signal-regulated kinase (MEK-ERK) and stress-activated (p38 kinase and JNK) Mitogen-activated protein kinases (MAPK) pathways. In the cells, MytiLec treatment triggered expression of tumor necrosis factor (TNF)-α (a ligand of death receptor-dependent apoptosis) and activation of mitochondria-controlling caspase-9 (initiator caspase) and caspase-3 (activator caspase). Experiments using the specific MEK inhibitor U0126 showed that MytiLec-induced phosphorylation of the MEK-ERK pathway up-regulated expression of the cyclin-dependent kinase inhibitor p21, leading to cell cycle arrest and TNF-α production. Activation of caspase-3 by MytiLec appeared to be regulated by multiple different pathways. Our findings, taken together, indicate that the novel R-type lectin MytiLec initiates programmed cell death of Burkitt’s lymphoma cells through multiple pathways (MAPK cascade, death receptor signaling; caspase activation) based on interaction of the lectin with Gb3-containing glycosphingolipid-enriched microdomains on the cell surface. 相似文献