Characteristic upregulation of glucose-regulated protein 78 in an early lesion negative for hitherto established cytochemical markers in rat hepatocarcinogenesis     

Sukata T  Uwagawa S  Ozaki K  Sumida K  Kushida M  Kakehashi A  Wanibuchi H  Miyata K  Ogata K  Fukushima S 《Journal of toxicologic pathology》2009,22(4):281-288

Previously, we reported α(2)-macroglobulin (α(2)M) to be a novel marker characteristic of rat hepatocellular preneoplastic and neoplastic lesions negative for hitherto well-established markers. In the present study, we further examined other candidate markers with specificity for the same type of lesions. Glutathione S-transferase-placental form (GST-P)-negative hepatocellular altered foci (HAF) were generated using a two-stage (initiation and promotion) carcinogenesis protocol with N,N-diethylnitrosamine (DEN) and either Wy-14,643 or clofibrate, two peroxisome proliferators. Microarray analysis using total RNAs isolated from laser-microdissected GST-P-negative HAF (amphophilic cell foci) and adjacent normal tissues was conducted along with immunohistochemistry and real-time RT-PCR. Staining for glucose-regulated protein 78 (GRP78) was detected in GST-P-negative HAF and hepatocellular adenomas, and slightly increased GRP78 mRNA expression was observed in the lesions by real-time RT-PCR analysis. Thus, an early increase of GRP78 expression in hepatocarcinogenesis is likely a feature of the amphophilic subset of HAF.  相似文献   

Effect of feeding rotifers enriched with taurine on the growth and survival of larval amberjack Seriola dumerili     

Hiroyuki Matsunari  Hiroshi Hashimoto  Takashi Iwasaki  Kentaro Oda  Yoshitsugu Masuda  Hitoshi Imaizumi  Kazuhisa Teruya  Hirofumi Furuita  Takeshi Yamamoto  Kazuhisa Hamada  Keiichi Mushiake 《Fisheries Science》2013,79(5):815-821

The effect of feeding rotifers enriched with taurine on the growth performance and survival of larval amberjack Seriola dumerili was investigated. Rotifers were enriched with a commercial taurine supplement at four levels (0, 200, 400, and 800 mg/l). The larvae were fed the enriched rotifers in triplicate from 3 days post-hatch for 7 days under static conditions. The average taurine contents of the rotifers were 1.5, 2.7, 4.2, and 7.2 mg/g dry matter, respectively. The growth of the fish fed rotifers enriched with the taurine supplement at 800 mg/l was significantly (P < 0.05) improved compared with that of the fish fed the rotifers without taurine enrichment. The survival rate improved proportionally up to a taurine supplement level of 400 mg/l, but no significant differences in survival were observed among treatments. The fraction of the larvae with inflated swim bladders did not vary significantly between treatments. Taurine content in the whole fish body increased with the taurine level in the rotifers. These results suggest that taurine enrichment of rotifers is an effective method of enhancing the growth of amberjack larvae.  相似文献   

Effects of docosahexaenoic acid on growth,survival and swim bladder inflation of larval amberjack ( Seriola dumerili, Risso)     

Hiroyuki Matsunari  Hiroshi Hashimoto  Kentaro Oda  Yoshitsugu Masuda  Hitoshi Imaizumi  Kazuhisa Teruya  Hirofumi Furuita  Takeshi Yamamoto  Kazuhisa Hamada  Keiichi Mushiake 《Aquaculture Research》2013,44(11):1696-1705

The effect of docosahexaenoic acid (DHA) on the growth performance, survival and swim bladder inflation of larval Seriola dumerili during the rotifer feeding period was investigated in two feeding experiments. Amberjack larvae at 3 day post hatching were fed rotifers enriched with (1) freshwater C hlorella (Chlo), (2) a mixture (2:1, v/v) of Chlo and DHA‐enriched C hlorella (DHA‐Chlo), (3) DHA‐Chlo and (4) DHA‐Chlo and commercial DHA emulsion, in triplicate for 7 days. The average DHA contents of the rotifers were 0.0, 0.4, 1.0 and 1.9 mg g^?1 DM respectively. The survival rate was improved by the enrichment of rotifers with DHA‐Chlo alone, and DHA‐Chlo and emulsion. Growth and swim bladder inflation of fish fed rotifers enriched with DHA‐Chlo were significantly (P < 0.05) improved, however, with increased levels of DHA further improvement was not found. DHA content in the larval whole body proportionally increased with the DHA level in the rotifers. These results suggest that DHA enrichment of rotifers is effective to improve the growth, survival rate and swim bladder inflation of amberjack larvae. The DHA requirement of amberjack larvae is estimated to be 1.5 mg g^?1 on a dry matter basis of rotifers.  相似文献   

Temperature dependences of the dynamic viscoelastic properties of wood and acetylated wood swollen by water or organic liquids     

Yuka Miyoshi  Ai Sakae  Nao Arimura  Keisuke Kojiro  Yuzo Furuta 《Journal of Wood Science》2018,64(2):157-163

In this study, to summarize the changes of thermal-softening behaviors of wood and acetylated wood due to differences in the kinds of swelling liquids, the following measurements were conducted. Untreated and acetylated wood samples were swollen by various liquids and the temperature dependences of the dynamic viscoelastic properties were measured after the heating and cooling histories were unified among the samples. The results obtained are as follows. Untreated samples swollen by high-polarity liquid had lower peak temperature of tanδ, however acetylated samples had higher peak temperature of tanδ than those of untreated wood. On the other hand, untreated wood samples swollen by low-polarity liquid had higher peak temperature of tanδ, however acetylated samples had lower peak temperature of tanδ than those of untreated wood. The amount of swelling is determined by interaction between wood and liquid due to proton-accepting power and molar volumes of liquid and so on, therefore the peak temperature of tanδ and degree of reduction in dynamic elastic modulus (E´) with increasing temperature were corresponded to the amount of swelling.  相似文献   

Pore structure and the properties of electric double layer capacitor electrode of bamboo-derived activated carbon prepared by superheated steam     

Keisuke Kikuchi  Rie Yamashita  Satoshi Sakuragawa  Keigo Hasumi  Yasuhiro Mukai  Hiroya Kobayakawa  Shojiro Wakabayashi  Yukie Saito 《Journal of Wood Science》2018,64(5):642-649

Bamboo-derived activated carbon prepared by superheated steam (BAC) exhibited performance for utilization as an electric double layer capacitor (EDLC) electrode. Pore structure and EDLC performances were investigated by comparison with phenol resin-derived activated carbon (MSP-20), which is commercially available and often used for the purpose. The nitrogen adsorption isotherm showed that BAC had a large BET-specific surface area of 1268 g/m² with a developed pore structure, especially of the mesopore, in comparison with MSP-20. It is considered that inherent ash in bamboo promoted activation, in addition to physical activation by superheated steam. Capacitance per electrode volume (C_V) was 52 F/cm³ with BAC. Because the density of BAC is high (0.78 g/cm³) compared with that of MSP-20 (0.58 g/cm³), sufficient C_V for usage was obtained, although the capacitance per electrode mass (C_M) at 5 mA/cm² itself of BAC (67 F/g) was lower than that of MSP-20 (126 F/g). With IR drop, the resistance value of BAC (9.7 Ω) was lower than that of MSP-20 (10.5 Ω). Especially, the diffusion resistance of BAC disclosed to be smaller than that of MSP-20. These results indicated that BAC produced by steam activation is a promising material with a pore structure suitable for ion transfer in EDLC.  相似文献   

Effects of density and anatomical feature on mechanical properties of various wood species in lateral tension     

Yuka Miyoshi  Keisuke Kojiro  Yuzo Furuta 《Journal of Wood Science》2018,64(5):509-514

This study is focused on what factor mainly affects the mechanical properties of each wood species in the lateral direction. At first, the influence of the density which is closely related to mechanical properties in the longitudinal direction was also researched in the lateral direction. Thus, the elastic modulus, strength, and failure strain in the lateral tension were measured using thin cross-sectional samples of softwoods and hardwoods, having wide varieties in the density and anatomical features. The results obtained are as follows. The linear relationship between the density and the elastic modulus which has been verified in the longitudinal direction was not observed in the lateral tension for the samples with the annual ring inclination of 90°, which samples were influenced by ray arranged parallel to the tensile direction. However, samples with the annual ring inclination of 45° showed the high correlation between the density and the elastic modulus due to the shearing deformation of the cell shape. On the other hand, the proportional relationship between the elastic modulus and strength which has been verified in the longitudinal direction was not observed in the lateral tension except for the samples with the annual ring inclination of 90°. From the results obtained, it was revealed that the mechanical properties of wood in the lateral direction were significantly affected not only by the density but also by the structural features such as deformation of cell shapes, arrangement of ray or vessels, and the degree of the transition from the earlywood to the latewood.  相似文献   

Effects of β‐carotene‐enriched dry carrots on β‐carotene status and colostral immunoglobulin in β‐carotene‐deficient Japanese Black cows          下载免费PDF全文

Yoshitaka Nishijima  Saya Taniguchi  Shuntaro Ikeda  Kenichi Yoshitani  Takashi Hamano  Hiroshi Tani  Masahiko Fujita  Kenji Murakami  Keisuke Kogusa  Kenji Sato  Miki Sugimoto  Shinichi Kume 《Animal Science Journal》2017,88(4):653-658

Data from 18 β‐carotene‐deficient Japanese Black cows were collected to clarify the effects of feeding β‐carotene‐enriched dry carrots on β‐carotene status and colostral immunoglobulin (Ig) in cows. Cows were assigned to control or carrot groups from 3 weeks before the expected calving date to parturition, and supplemental β‐carotene from dry carrots was 138 mg/day in the carrot group. Plasma β‐carotene concentrations in the control and carrot groups at parturition were 95 and 120 μg/dL, and feeding dry carrots slightly improved plasma β‐carotene at parturition. Feeding dry carrots increased colostral IgA concentrations in cows and tended to increase colostral IgG₁, but colostral IgM, IgG₂, β‐carotene and vitamin A were not affected by the treatment. Feeding dry carrots had no effects on plasma IgG₁, IgA and IgM concentrations in cows, but plasma IgG₁ concentrations decreased rapidly from 3 weeks before the expected calving date to parturition. These results indicate that feeding β‐carotene‐enriched dry carrots is effective to enhance colostral IgA and IgG₁ concentrations in β‐carotene‐deficient cows.  相似文献   

Site preference and occurrence patterns of <Emphasis Type="Italic">Picea jezoensis</Emphasis> and <Emphasis Type="Italic">Abies sachalinensis</Emphasis> on decayed logs in natural coniferous forests in Hokkaido,northern Japan     

Keisuke Yano  Masato Shibuya 《Journal of Forest Research》2010,15(2):108-114

The objectives of this study were to investigate differences in the site preferences of seedlings of Picea jezoensis and Abies sachalinensis on decayed logs, and to examine the occurrence patterns of seedlings and saplings of the two species and whether they occur together or separately on logs. We characterized the habitats of 1–2-year-old seedlings of the two species on logs and examined the relationship of the two species on logs by growth stages in two plots. One plot had been disturbed about 50 years ago whereas the other had not for a long time. Although the thickness of moss and the litter layer in the habitats of 1–2-year-old seedlings were significantly different between the two species, the two species could occur together. In one study plot, seedlings and saplings of the two species occurred together. The initial occurrence pattern of the seedlings affected the occurrence patterns of the saplings on logs. The occurrence patterns of the seedlings and saplings of the two species on logs seemed to be affected by the abundance of seed trees. In the other study plot saplings of the two species occurred separately, but one species was not always competitively superior to the other species. Disturbance history affected the occurrence patterns of the saplings of the two species on decayed logs at the two study plots. Consequently, it is concluded that seed dispersal and the abundance of available logs, which are usually affected by disturbance, are significant factors in the natural regeneration of conifers in Hokkaido.  相似文献   

Effects of muscle type on beef taste‐traits assessed by an electric sensing system     

Koichi CHIKUNI  Mika OE  Keisuke SASAKI  Masahiro SHIBATA  Ikuyo NAKAJIMA  Koichi OJIMA  Susumu MUROYA 《Animal Science Journal》2010,81(5):600-605

To assess the role of muscle fiber type in beef taste‐traits, we analyzed cooked meats from bovine masseter, diaphragm, psoas major, longissimus thoracis, and semitendinosus muscles with an electric taste sensing system (INSENT SA402B). The system is composed of five taste sensors of polymer membranes fixing different lipids. The sensors, CT0, CA0, AAE, C00 and AE1 are designed to respond to the individual tastes of salty, sour, umami, bitter and astringent, respectively. The system found significant differences in the converted outputs of CA0 (cvCA0), C00 (cvC00) and AE1 (cvAE1) among the bovine muscles. The slow‐type muscles (masseter and diaphragm) showed lower cvCA0, higher cvC00, and higher cvAE1 than did the fast‐type muscles (psoas major, longissimus thoracis, and semitendinosus). Lactic acid content was different among muscle types and was highly related to the cvCA0 output and pH. carbonyl compounds and free fatty acids were higher in the slow‐type muscles. Free fatty acids were major components causing the difference in the C00 output among the muscle types. Iron content was also different among the muscle types and related to the cvC00 and cvAE1 outputs. These results suggested that the muscle fiber type affects the beef taste characteristics.  相似文献   

Anthracnose of Madagascar periwinkle caused by species belonging to the Colletotrichum gloeosporioides species complex     

Keisuke Tomioka  Junji Nishikawa  Jouji Moriwaki  Toyozo Sato 《Journal of General Plant Pathology》2013,79(5):374-377

Severe blight of stems was found on Madagascar periwinkle (Catharanthus roseus) in Shizuoka and Okinawa prefectures, Japan, in July 2003 and September 2004, respectively. Similar fungi were isolated from the diseased plants. The isolated fungi caused the disease after inoculation and subsequently were reisolated from diseased tissues. The fungi were identified as species closely related to C. siamense or C. tropicale according to sequences of the β-tubulin-2 gene, indicating that they belong to the Colletotrichum gloeosporioides species complex. This is the first report specifying the genetic relationships of the pathogens of Madagascar periwinkle anthracnose in the complex.  相似文献