Prophylaxis of Bordetella bronchiseptica infection in guinea pigs by vaccination   总被引：4，自引：0，他引：4  

M Nakagawa  H Yoda  T Muto  K Imaizumi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1974,36(1):33-42

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Seed germination responses to temperature and water availability in weedy rice     

Monrawee Fukuda  Toshiyuki Imaizumi  Akira Koarai 《Pest management science》2023,79(2):870-880

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Finding of a highly efficient ZFN pair for Aqpep gene functioning in murine zygotes     

Wataru FUJII  Asuka ONUMA  Shin YOSHIOKA  Keisuke NAGASHIMA  Koji SUGIURA  Kunihiko NAITO 《The Journal of reproduction and development》2015,61(6):589-593

The generation efficiencies of mutation-induced mice when using engineered zinc-finger nucleases (ZFNs) have been generally 10 to 20% of obtained pups in previous studies. The discovery of high-affinity DNA-binding modules can contribute to the generation of various kinds of novel artificial chromatin-targeting tools, such as zinc-finger acetyltransferases, zinc-finger histone kinases and so on, as well as improvement of reported zinc-finger recombinases and zinc-finger methyltransferases. Here, we report a novel ZFN pair that has a highly efficient mutation-induction ability in murine zygotes. The ZFN pair induced mutations in all obtained mice in the target locus, exon 17 of aminopeptidase Q gene, and almost all of the pups had biallelic mutations. This high efficiency was also shown in the plasmid DNA transfected in a cultured human cell line. The induced mutations were inherited normally in the next generation. The zinc-finger modules of this ZFN pair are expected to contribute to the development of novel ZF-attached chromatin-targeting tools.  相似文献   

Comparative study of dermal components and plasma TGF-β1 levels in Slc39a13/Zip13-KO mice     

Takuya HIROSE  Takayuki OGURA  Keisuke TANAKA  Jun MINAGUCHI  Takeshi YAMAUCHI  Toshiyuki FUKADA  Yoh-ichi KOYAMA  Kazushige TAKEHANA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1385-1389

Ehlers-Danlos syndrome (EDS) is a group of disorders caused by abnormalities that are identified in the extracellular matrix. Transforming growth factor-β1 (TGF-β1) plays a crucial role in formation of the extracellular matrix. It has been reported that the loss of function of zinc transporter ZRT/IRT-like protein 13 (ZIP13) causes the spondylocheiro dysplastic form of EDS (SCD-EDS: OMIM 612350), in which dysregulation of the TGF-β1 signaling pathway is observed, although the relationship between the dermis abnormalities and peripheral TGF-β1 level has been unclear. We investigated the characteristics of the dermis of the Zip13-knockout (KO) mouse, an animal model for SCD-EDS. Both the ratio of dermatan sulfate (DS) in glycosaminoglycan (GAG) components and the amount of collagen were decreased, and there were very few collagen fibrils with diameters of more than 150 nm in Zip13-KO mice dermis. We also found that the TGF-β1 level was significantly higher in Zip13-KO mice serum. These results suggest that collagen synthesis and collagen fibril fusion might be impaired in Zip13-KO mice and that the possible decrease of decorin level by reduction of the DS ratio probably caused an increase of free TGF-β1 in Zip13-KO mice. In conclusion, skin fragility due to defective ZIP13 protein may be attributable to impaired extracellular matrix synthesis accompanied by abnormal peripheral TGF-β homeostasis.  相似文献   

Monitoring saline intrusion in the Ayeyarwady Delta,Myanmar, using data from the Sentinel-2 satellite mission     

Sakai  Toru  Omori  Keisuke  Oo  Aung Naing  Zaw  Yan Naung 《Paddy and Water Environment》2021,19(2):283-294

Paddy and Water Environment - Summer rice cannot be grown near the coast of the Ayeyarwady Delta, Myanmar, because of the high salinity in river water during the dry season. This means that saline...  相似文献   

Toward the development of a weather index insurance for rice farmers in the coastal region of Myanmar     

Furuya  Jun  Omori  Keisuke  Aizaki  Hideo 《Paddy and Water Environment》2021,19(2):255-260

Paddy and Water Environment - Climate change is expected to exacerbate damage to agricultural production from natural disasters. Examination of measures to adapt to the damage represents an urgent...  相似文献   

Irradiation history of Itokawa regolith material deduced from noble gases in the Hayabusa samples     

Nagao K  Okazaki R  Nakamura T  Miura YN  Osawa T  Bajo K  Matsuda S  Ebihara M  Ireland TR  Kitajima F  Naraoka H  Noguchi T  Tsuchiyama A  Yurimoto H  Zolensky ME  Uesugi M  Shirai K  Abe M  Yada T  Ishibashi Y  Fujimura A  Mukai T  Ueno M  Okada T  Yoshikawa M  Kawaguchi J 《Science (New York, N.Y.)》2011,333(6046):1128-1131

Noble gas isotopes were measured in three rocky grains from asteroid Itokawa to elucidate a history of irradiation from cosmic rays and solar wind on its surface. Large amounts of solar helium (He), neon (Ne), and argon (Ar) trapped in various depths in the grains were observed, which can be explained by multiple implantations of solar wind particles into the grains, combined with preferential He loss caused by frictional wear of space-weathered rims on the grains. Short residence time of less than 8 million years was implied for the grains by an estimate on cosmic-ray-produced (21)Ne. Our results suggest that Itokawa is continuously losing its surface materials into space at a rate of tens of centimeters per million years. The lifetime of Itokawa should be much shorter than the age of our solar system.  相似文献   

Nitrogen isotope fractionation during nitrogen transport from ectomycorrhizal fungi,Suillus granulatus,to the host plant,Pinus densiflora     

Ayato Kohzu  Takahiro Tateishi  Akiyoshi Yamada  Keisuke Koba  Eitaro Wada 《Soil Science and Plant Nutrition》2013,59(3):733-739

The ¹⁵N natural abundance in Pinus densiflora Sieb. et Zucco that had been inoculated and not inoculated with ectomycorrhizal fungi (Suillus granulatus (L.:Fr.) O. Kuntze) was compared. The inoculated pine needles showed a lower δ¹⁵N value, while the uninoculated ones showed a higher δ¹⁵N value. Higher δ¹⁵N values in the mycelial mat of the ectomycorrhizal fungi compared to those of the inoculated pine needles were also observed. These facts indicate that nitrogen isotope fractionation occurred during the nitrogen transport from mycorrhizal fungi to the host plants.  相似文献   

Iron-deficiency response and expression of genes related to iron homeostasis in poplars     

Hiroshi Masuda  May Sann Aung  Keisuke Maeda  Takanori Kobayashi  Naoki Takata  Toru Taniguchi 《Soil Science and Plant Nutrition》2013,59(5):576-588

Iron (Fe) deficiency is a serious agricultural problem, especially in calcareous soils, which are distributed worldwide. Poplar trees are an important biomass plant, and overcoming Fe deficiency in poplars will increase biomass productivity worldwide. The poplar Fe-deficiency response and the genes involved in poplar Fe homeostasis remain largely unknown. To identify these genes and processes, we cultivated poplar plants under Fe-deficient conditions, both in calcareous soil and hydroponically, and analyzed their growth rates, leaf Soil and Plant Analyzer Development (SPAD) values, and metal concentrations. The data clearly showed that poplars have notable growth defects in both calcareous soil and a Fe-deficient hydroponic culture. They exhibited serious chlorosis of young leaves after 3 weeks of Fe-deficient hydroponic culture. The Fe concentrations in old leaves with high SPAD values were markedly lower in Fe-deficient poplars, suggesting that poplars may have good translocation capability from old to new leaves. The Zn concentration in new leaves increased in Fe-deficient poplars. The pH of the hydroponic solution decreased in the Fe-deficient culture compared to the Fe-sufficient culture. This finding shows that poplars may be able to adjust the pH of a culture solution to better take up Fe. We also analyzed the expression of Fe homeostasis-related genes in the roots and leaves of Fe-sufficient and Fe-deficient poplars. Our results demonstrate that PtIRT1, PtNAS2, PtFRO2, PtFRO5, and PtFIT were induced in Fe-deficient roots. PtYSL2 and PtNAS4 were induced in Fe-deficient leaves. PtYSL3 was induced in both Fe-deficient leaves and roots. These genes may be involved in the Fe uptake and/or translocation mechanisms in poplars under Fe-deficient conditions. Our results will increase a better understanding of the Fe-deficiency response of poplars and hence improve the breeding of Fe-deficiency-tolerant poplars for improved biomass production, the greening of high pH soils, and combatting global warming.  相似文献   

Internal fruit rot of sweet pepper caused by Fusarium lactis in Japan and fungal pathogenicity on tomato and eggplant fruits     

Sekiguchi  Hiroyuki  Masunaka  Akira  Nomiyama  Koji  Tomioka  Keisuke 《Journal of General Plant Pathology》2021,87(5):326-329

Journal of General Plant Pathology - A postharvest rot of ripe sweet pepper fruits was found in Kagawa Prefecture in western Japan in October 2002. A fungus, isolated repeatedly from the diseased...  相似文献