Expression of bovine cytokines in Escherichia coli   总被引：7，自引：0，他引：7  

Kashima T  Morishita A  Iwata H  Maeda K  Inoue T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(2):171-173

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Four-legged broiler chicken with two cloacae and three ceca     

Ebako GM  Morishita TY  Mattoon JS 《Avian diseases》2002,46(1):234-238

This case report examines an Arbor Acres female x Peterson male crossbred 6-wk-old female broiler chicken with four legs, two cloacae, and three ceca. The intestines occupied the caudodorsal portion of the abdominal cavity with three ceca attached to the terminal end of the ileum. The left lateral cecum was larger and had a divided distal end that terminated into 2-cm-long blind sacs. The rectum was dilated and divided into two cloacae that each opened on the dorsolateral aspect of both the right and left cloacal elevator muscles. The two extra legs were attached to the pygostyle by cartilage and skin tissue. Both femoral heads of the extraneous legs were fused together. The extra legs were smaller in size (approximately 17 cm in length) compared with the normal legs (approximately 22 cm). The femoral heads of the two normal legs were disarticulated from the acetabulum.  相似文献   

Evaluation of Pasteurella multocida isolated from rabbits by capsular typing, somatic serotyping, and restriction endonuclease analysis.     

Amna B El Tayeb  Teresa Y Morishita  Elisabeth J Angrick 《Journal of veterinary diagnostic investigation》2004,16(2):121-125

The goal of this study was to characterize Pasteurella multocida isolated from rabbits. Five hundred and fifty-three apparently healthy rabbits were sampled for this study. Nasal swabs were collected from each rabbit for P. multocida isolation and identification. Isolates were further characterized by capsular and somatic antigens and genomic DNA fingerprinting. Thirty-nine P. multocida isolates were recovered from 553 rabbits (7%). Capsular typing was done by depolymerization of P. multocida capsule by Staphylococcus aureus hyaluronidase and by disc diffusion with mucopolysaccharidase enzymes (heparinase III, chondroitinase AC, and hyaluronidase). Thirty-one (79%) of the isolates were capsular type A, and 8 isolates (21%) had untypable (UT) capsules. The gel-diffusion precipitin test was used to determine the somatic type of P. multocida isolates. Nineteen isolates were somatic serotype 3 (49%), 12 were serotype 1 (31%), 1 was serotype 2, 2 were serotype 5, 2 were serotype 12 with a weak reaction to antiserum raised against serotype 7 (5%), and 1 was serotype 4. Two of the isolates (5%) were UT. Restriction endonuclease analysis of the DNA of the isolates revealed 7 distinct profiles by digestion with HindIII, and 12 profiles were obtained with HpaII, whereas digestion with EcoRI did not differentiate between any of the P. multocida DNA isolates studied. The DNA restriction endonuclease enzyme HpaII was found more useful for differentiating between DNA fingerprints of P. multocida rabbit isolates. However, no correlation between capsular type, somatic serotypes, and DNA fingerprints was seen in this study.  相似文献   

Effects of elevated atmospheric CO2 concentration on the nutrient uptake characteristics of Japanese larch (Larix kaempferi)     

Shinano T  Yamamoto T  Tawaraya K  Tadokoro M  Koike T  Osaki M 《Tree physiology》2007,27(1):97-104

We evaluated the response of Japanese larch (Larix kaempferi Sieb. & Zucc.) to elevated atmospheric CO(2) concentration ([CO(2)]) (689 +/- 75 ppm in 2002 and 697 +/- 90 ppm in 2003) over 2 years in a field experiment with open-top chambers. Root activity was assessed as nitrogen, phosphorus and potassium uptake rates estimated from successive measurements of absorbed amounts. Dry matter production of whole plants was unaffected by elevated [CO(2)] in the first year of treatment, but increased significantly in response to elevated [CO(2)] in the second year. In contrast, elevated [CO(2)] increased the root to shoot ratio and fine root dry mass in the first year, but not in the second year. Elevated [CO(2)] had no effect on tissue N, P and K concentrations. Uptake rates of N, P and K correlated with whole-plant relative growth rates, but were unaffected by growth [CO(2)], as was ectomycorrhizal colonization, a factor assumed to be important for nutrient uptake in trees. We conclude that improved growth of Larix kaempferi in response to elevated [CO(2)] is accompanied by increased root biomass, but not by increased root activity.  相似文献   

Spatial and seasonal variations of radiocesium concentrations in an algae-grazing annual fish,ayu Plecoglossus altivelis collected from Fukushima Prefecture in 2014     

Morishita  Daigo  Wada  Toshihiro  Noda  Takuji  Tomiya  Atsushi  Enomoto  Masahiro  Sato  Toshiyuki  Suzuki  Shunji  Kawata  Gyo 《Fisheries Science》2019,85(3):561-569

Fisheries Science - To elucidate spatial and seasonal variations of radiocesium (134Cs and 137Cs) concentrations in ayu Plecoglossus altivelis, amphidromous fish samples were collected both from...  相似文献   

Sex discrimination of cultured greater amberjack Seriola dumerili using steroid hormones     

Aoki  Ryuichiro  Chuda  Hisashi  Washio  Youhei  Masuma  Shukei  Kato  Keitaro 《Fisheries Science》2020,86(1):57-64

Fisheries Science - The aquaculture of greater amberjack Seriola dumerili is of considerable research interest worldwide. The larviculture methods employed to culture this species, however, are...  相似文献   

Flow field control via aeration adjustment for the enhancement of larval survival of the kelp grouper Epinephelus bruneus (Perciformes:Serranidae)     

Ching Fui Fui  Akira Miura  Yoshizumi Nakagawa  Keitaro Kato  Shigeharu Senoo  Wataru Sakamoto  Kenji Takii  Shigeru Miyashita 《Aquaculture Research》2014,45(5):874-881

Flow field control via aeration adjustment for the enhancement of larval survival of the kelp grouper Epinephelus bruneus was examined. Aeration rate of 300 mL min^?1 was introduced during daylight (07:00–19:00 hours) and adjusted to 0, 300 and 900 mL min^?1 at night (19:00–07:00 hours). Larval sinking velocity±SD increased from 0.08 ± 0.05 to 0.26 ± 0.24 cm sec^?1 from 4 to 12 days after hatching (DAH), indicating their susceptibility to sink. Larvae reared in 300 mLmin^?1 attained the highest survival rate at 24.9 ± 3.4%, but remained significantly smaller in growth: 4.54 ± 0.56 mm compared with 4.82 ± 0.53 mm in 900 mL min^?1. The flow field in 300 and 900 mL min^?1 was at 10–20 and 15–25 cm above the bottom of the tank and 8.0 and 1.0 cm beneath the water surface. A favourable rearing condition was observed in 300 mL min^?1 as larvae were away from the bottom and surface areas, thus preventing them from dying due to sinking and surface tension‐related death (STRD). Although sinking death was decreased with an increasing aeration rate, the stronger flow had increased larval susceptibility to STRD. Our findings suggest that aeration at 300 mL min^?1 could enhance larval survival by reducing both sinking death and STRD.  相似文献   

Aeration rate adjustment at night to prevent sinking syndrome‐related death in the tiger grouper Epinephelus fuscoguttatus (Perciformes:Serranidae) larvae          下载免费PDF全文

Ching Fui Fui  Akira Miura  Yoshizumi Nakagawa  Keitaro Kato  Wataru Sakamoto  Kenji Takii  Shigeru Miyashita  Shigeharu Senoo 《Aquaculture Research》2016,47(1):165-175

The effects of different aeration rates at night to prevent sinking syndrome‐related death (SSRD) of the tiger grouper, Epinephelus fuscoguttatus were examined. The aeration rates were fixed at 300 mL min^?1 at daytime (07:00–19:00 hours) and regulated to 0, 300 and 900 mL min^?1 at night (19:00–07:00 hours). Larval survival, growth, feeding intake, sinking velocity, distribution and behaviour, stress level, surface tension‐related death (STRD) and flow velocity distribution were assessed. The occurrence of SSRD in the tiger grouper was observed through the accelerated sinking velocity (Vl) (from 0.15 ± 0.09 cm s^?1 at 4 days AH to 0.41 ± 0.09 cm s^?1 at 12 days AH) coupled with larval passive swimming behaviour at night‐time. On the final day of experiment (15 days AH), larvae reared in 900 mL min^?1 at night had attained significantly higher (P < 0.05) survival (34.4 ± 5.5%), growth (5.8 ± 0.5 mm) and feeding intake (60.46 ± 6.98 ind. larva^?1). A favourable flow field for the tiger grouper was produced in 900 mL min^?1 at night‐time, in which larvae were transported 15–25 cm above the tank bottom and 1.0 cm beneath the water surface. Under these night‐time rearing conditions, larval stress level and number of STRD reared in 900 mL min^?1 compared with those observed in 300 mL min^?1 remained insignificant, indicating that strong turbulence of flow velocity was not detrimental for larvae. Our findings recommend aeration at 900 mL min^?1 at night as this could improve larval survival by reducing SSRD.  相似文献   

Correction to: Spatial and seasonal variations of radiocesium concentrations in an algae-grazing annual fish,ayu Plecoglossus altivelis collected from Fukushima Prefecture in 2014     

Morishita  Daigo  Wada  Toshihiro  Noda  Takuji  Tomiya  Atsushi  Enomoto  Masahiro  Sato  Toshiyuki  Suzuki  Shunji  Kawata  Gyo 《Fisheries Science》2019,85(3):571-571

Fisheries Science - In the original publication the caption of Fig.&nbsp;6a and 6b is incorrectly published.  相似文献   

Involvement of neuropeptide Y in hyperphagia in human growth hormone transgenic rats     

Hozumi H  Yamanouchi K  Nishihara M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(9):959-965

We have previously produced human growth hormone (hGH) transgenic (TG) rats that show low circulating levels of both hGH and endogenous rat GH. Although body length of the TG rats is normal, they develop hyperphagia and severe obesity. The present study was undertaken to elucidate the causes of hyperphagia in the TG rats by focusing on temporal changes in plasma ghrelin levels and hypothalamic neuropeptide Y (NPY) contents. In both wild-type (WT) and TG rats, the highest value of plasma ghrelin levels was observed just before the dark phase, and thereafter plasma ghrelin levels were maintained higher in the TG than WT rats. Although NPY contents also showed the peak level just before the dark phase in both the arcuate (ARC) and paraventricular nuclei (PVN) of the hypothalamus, the values in the ARC, but not the PVN, of the TG rats was always lower than those of the WT rats, suggesting increased transport of NPY from the ARC to PVN in the TG rats. In addition, treatment with antagonists for Y1 and Y5 receptors for NPY reduced food intake much more effectively in the TG than WT rats. Intermittent treatment with recombinant hGH for a week significantly decreased food consumption, adipose tissue weight and plasma triglyceride concentrations in the TG rats. These results suggest that, in the TG rats, insufficiency in circulating GH stimulates the ghrelin-NPY system with a resultant increase in food intake.  相似文献