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151.
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153.
Etsuko KASUYA Ken‐ichi YAYOU Tsutomu HASHIZUME Sayuki KITAGAWA Madoka SUTOH 《Animal Science Journal》2010,81(3):345-351
To clarify the role of serotonin (5‐HT) in the regulatory mechanism of L‐tryptophan (TRP)‐induced growth hormone (GH) secretion in cattle, changes in 5‐HT concentrations in the cerebrospinal fluid (CSF) in the third ventricle (3V) and GH in plasma before and after the peripheral infusion of TRP were determined simultaneously. The direct effect of TRP on GH release from the dispersed anterior pituitary cells was also assessed. A chronic cannula was placed in 3V by stereotaxic surgery, then CSF and blood were withdrawn under physiological conditions. TRP (38.5 mg/kg BW) was infused through an intravenous catheter from 12.00 to 14.00 hours and CSF and blood sampling were performed from 11.00 to 18.00 hours at 1‐h intervals. The concentration of 5‐HT in CSF was determined by high‐performance liquid chromatography with electrochemical detection. GH, melatonin (MEL), and cortisol (CORT) concentrations were measured by radio‐immunoassay and enzyme‐immunoassay. Concentrations of 5‐HT were increased by TRP infusion. The TRP infusion significantly increased GH release. On the other hand, TRP did not stimulate GH release from the bovine pituitary cells. MEL and CORT concentrations were not altered by TRP infusion. These results suggest that TRP induced GH release via the activation of serotonergic neurons in cattle. 相似文献
154.
Flavones are abundant in parsley and celery and possess unique anti-inflammatory properties in vitro and in animal models. However, their bioavailability and bioactivity depend in part on the conjugation of sugars and other functional groups to the flavone core. The effects of juice extraction, acidification, thermal processing, and endogenous enzymes on flavone glycoside profile and concentration in both parsley and celery were investigated. Parsley yielded 72% juice with 64% of the total flavones extracted, whereas celery yielded 79% juice with 56% of flavones extracted. Fresh parsley juice averaged 281 mg flavones/100 g and fresh celery juice, 28.5 mg/100 g. Flavones in steamed parsley and celery were predominantly malonyl apiosylglucoside conjugates, whereas those in fresh samples were primarily apiosylglucoside conjugates; this was apparently the result of endogenous malonyl esterases. Acidification and thermal processing of celery converted flavone apiosylglucosides to flavone glucosides, which may affect the intestinal absorption and metabolism of these compounds. 相似文献
155.
Alberta’s oil sands are located in the boreal forest where surface mining requires reconstruction of these landscapes using
waste saline and sodic overburden (SSOB) piles. The impact of these SSOB materials, however, on root development of planted
boreal species is unknown. The objective of this study was to examine the effect of SSOB material on the root distributions
of planted boreal species. Root distributions for planted mixedwood stands were measured using soil cores and compared with
soil physical and chemical properties on three reclaimed sites. Soil pH ranged from 6.1 to 7.5 across all three reclaimed
sites. Sodium adsorption ratio ranged from <30 in the SSOB at the youngest site to <4 at the oldest site while soil electrical
conductivity ranged from <12 and <4 dS m−1 in the SSOB at the youngest and oldest site, respectively. Root length distributions were concentrated in the upper 30 cm
of the soil profile and ranged from 0.96 to 7.99 cm cm−3. The roots were observed in the SSOB and accounted for 1.3% to 2.2% of the total root length in the profile. The root length
density was also negatively correlated with Na and EC at all sites. The root distributions on these young reclaimed sites
were similar to those from undisturbed boreal forest stands overlying saline soils, suggesting that root distributions on
these reclaimed sites appear to be unaffected by the SSOB; however, further monitoring will be required as the stand matures
to determine future impacts of the SSOB on forest productivity. 相似文献
156.
Effect of plastic-film mulching on leaching of nitrate nitrogen in an upland field converted from paddy 总被引:4,自引:0,他引:4
Tomokazu?HaraguchiEmail author Atsushi?Marui Kozue?Yuge Yoshisuke?Nakano Ken?Mori 《Paddy and Water Environment》2004,2(2):67-72
A lysimeter experiment was conducted to examine the effects of plastic film mulching on the leaching rate of nitrate nitrogen (NO3-N) from chemical fertilizer that was applied to an upland field that had been converted from paddy rice production. Leaching was monitored in two lysimeters filled with sandy loam soil, which contained low soil organic matter content, under different surface mulch conditions. One was mulched only on the ridge (ridge-mulch treatment) and another one was mulched fully, including the furrow, with black plastic film (full-mulch treatment). Chemical fertilizer was mixed into the top 0.2 m of soil in the two lysimeters before installing the mulch. After transplanting broccoli, the amount of subsurface discharge water and the NO3-N concentrations in the discharge water were measured every day. Larger NO3-N discharges occurred in the ridge-mulch treatment for three days after heavy rainfalls in which cumulative precipitation exceeded 10 mm, and the daily NO3-N load was twice as large as the full-mulch treatment. The differences in the amount of subsurface discharge water and NO3-N discharged between treatments were not significant when there was no rainfall. Cumulative NO3-N loads for the ridge- and full-mulch treatment during the last month of the experimental period were 0.246 and 0.195 g m–2, respectively. The effect of mulching on the reduction of NO3-N discharge rate was higher for the full-mulch treatment. This result showed that a plastic-film mulching system would be effective as an appropriate fertilizer management to reduce nitrate-leaching losses. 相似文献
157.
Sawai K Takahashi M Fujii T Moriyasu S Hirayama H Minamihashi A Hashizume T Onoe S 《The Journal of reproduction and development》2011,57(2):236-241
DNA methylation is an important factor for the regulation of gene expression in early embryos. It is well known that the satellite I sequence is more heavily methylated in bovine somatic cell nuclear transfer (NT-SC) embryos than in embryos derived from in vitro fertilization (IVF). However, the methylation status of bovine embryos obtained by other procedures is not well known. To clarify DNA methylation levels of bovine embryos obtained from various procedures, we examined satellite I sequences in bovine blastocyst (BC) embryos derived from NT-SC, NT using embryonic blastomeres (NT-EM), in vivo (Vivo), IVF and parthenogenetic treatment (PA). Furthermore, in order to evaluate the efficacy of DNA demethylation by the NT procedure, we determined the DNA methylation levels in bovine embryos in which NT was recapitulated (Re-NT). Although the DNA methylation levels in the NT-SC embryos were higher than those in the other embryos, the NT-EM embryos exhibited lower DNA methylation levels. The satellite I sequence in the NT-SC embryos was more demethylated than that in the donor cells. Although the DNA methylation level in the individual NT-SC embryos showed variation, the full-term developmental efficacy of these embryos were not different. These findings suggest that the methylation level of the satellite I sequence at the BC stage is not related to the abnormalities of bovine embryos produced by NT-SC. There was no difference in methylation levels between Re-NT and NT-SC embryos. Our results indicated that the DNA methylation status differed among embryos produced by various methods and that at least some of the demethylation of the donor cell genome occurred in the recipient cytoplast after NT-SC, but the demethylation ability of the NT procedure was noted in the first NT but not in the second NT. 相似文献
158.
Clarke JD Riedl K Bella D Schwartz SJ Stevens JF Ho E 《Journal of agricultural and food chemistry》2011,59(20):10955-10963
Increased consumption of cruciferous vegetables such as broccoli may reduce the risk of various cancers. Myrosinase is required to convert dietary glucosinolates from broccoli into bioactive isothiocyanates. We evaluated isothiocyanate excretion profiles in healthy subjects who consumed broccoli sprouts or broccoli supplement (no myrosinase) with equivalent glucosinolate content. Urinary metabolites of two major isothiocyanates, sulforaphane and erucin, were measured by liquid chromatography coupled with tandem mass spectrometry. Peak excretion of sulforaphane and erucin was higher and occurred sooner in subjects who consumed broccoli sprouts as compared to subjects who consumed the supplement. A subject-dependent shift in the ratio of urinary sulforaphane to erucin metabolites was observed in both groups, indicating conversion of sulforaphane to erucin. Lower histone deacetylase activity was observed in the peripheral blood mononuclear cells only in subjects consuming sprouts. Fresh broccoli sprouts differ from broccoli supplements in regards to excretion of isothiocyanates and bioactivity in human subjects. 相似文献
159.
Onda K Sugiyama M Niho K Sato R Arai S Kaneko K Ito S Muto M Suganuma T Wakao Y Wada Y 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(6):667-669
This study investigated the long-term survival of a calf with cervical ectopia cordis that grew normally, became pregnant, and calved normally. The cow showed normal cardiac function and absence of peripheral circulation abnormalities. This paper documents antemortem characteristics of the affected cow. 相似文献
160.