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排序方式: 共有231条查询结果,搜索用时 15 毫秒
91.
Mitsuo Horita Yasuhiro Suga Atsushi Ooshiro Kenichi Tsuchiya 《Journal of General Plant Pathology》2010,76(3):196-207
We assessed the geographic distribution, biovar, phylotype, DNA fingerprints (rep-PCR), and/or endoglucanase sequence of potato
bacterial wilt pathogen, Ralstonia solanacearum (Rs), in Japan. Rs has been isolated from potato fields in southwestern, warm, temperate regions. Of the 188 isolates, 74
belonged to biovar N2 (39%), 44 to biovar 3 (24%), and 70 to biovar 4 (37%). Biovars N2 and 4 strains were widely distributed,
from northern (Hokkaido) to southern (Okinawa) Japan. Based on the results of multiplex-PCR analysis, every potato strains
belonged to either phylotype I or IV. Phylotype I comprised both biovars 3 and 4 strains. On the other hand, phylotype IV
included biovar N2 strains. None of the strains belonged to phylotype II or III or biovar 1 or 2. Phylogenetic analysis based
on DNA fingerprints and endoglucanase gene sequences clarified the genetic diversity of the Japanese potato strains and the
close genetic relationship between the Japanese strains and the Asian strains in phylotypes I and IV. 相似文献
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94.
Rosemary Shrestha Kenichi Tsuchiya Su Jin Baek Hu Nam Bae Ingyu Hwang Jang Hyun Hur Chun Keun Lim 《Journal of General Plant Pathology》2005,71(3):211-220
Erwinia pyrifoliae, the causal pathogen of shoot blight in the Asian pear tree (Pyrus pyrifolia cv. Singo), is host-specific and endemic to Korea. To identify the genes associated with the hypersensitive response (HR) and pathogenicity, a genomic library of E. pyrifoliae WT3 was constructed, and the cosmid clone Escherichia coli (pCEP33) was selected. Sequence analysis of 19.7-kb pCEP33 determined disease-specific (dsp) region homolog and approximately 40% of the hrp genes, which included hrpW, hrpNEp, hrpV, hrpT, hrcC, hrpG, hrpF, and partial hrpE homologs, with respect to the cluster of Erwinia amylovora. Additionally, two open reading frames, ORFD and ORFE, were found downstream of the dspEF region. The results of the sequence analysis showed that the pCEP33 did not contain any hrp regulatory genes or most of the genes encoding components of the Hrp protein secretion system. The hrpNEp gene of E. pyrifoliae contained five intergenic nucleotide fragment insertions (INFIs) and produced the HR elicitor protein harpinEp, with a molecular mass of approximately 44kDa. The purified HrpNEp protein elicited faster and stronger HR when infiltrated into tobacco leaves than did HrpNEa from E. amylovora. To observe the role of the hrpL gene in the expression of HrpNEp, the pEL2 containing hrpL was used to transform E. coli (pCEP33). Expression of HrpNEp in E. coli (pCEP33 + pEPL2) was detected with an immunoblot using antiserum raised against HrpNEp, indicating a role of hrpL gene in enhancing the expression of HrpNEp. 相似文献
95.
Masuda K 《Veterinary immunology and immunopathology》2005,108(1-2):185-187
Sensitization to allergens of Japanese cedar pollen is known to cause canine atopic dermatitis as approximately 10% of atopic dogs in Japan were positive to the pollen allergen. Among the two major allergens of Japanese cedar pollen, since Cry j 1 is more important than Cry j 2 as an antigen to increase IgE in atopic dogs sensitized to Japanese cedar pollen, Cry j 1 can be a target for immunotherapy. In our study, efficacy of DNA vaccination with a plasmid containing the gene of a major allergen of Japanese cedar (Cryptomeria japnonica, CJ) pollen, Cry j 1, was examined using a dog model experimentally sensitized to CJ pollen allergen. Cry j 1 DNA plasmid and a vector plasmid (pCAGGS) were injected into six dogs and three dogs, respectively, five times with an interval of 1.5 month. After the treatment with Cry j 1 DNA plasmid, production of IgE against Cry j 1 decreased in four of the six dogs in the treatment group, whereas it increased in the three dogs of the control group. The reactivity to the pollen allergen in intradermal testing and provocation testing were obviously reduced in the treatment group, but not in the control group. The number of mast cells in alveolar area of the lung in the treatment group was smaller than that in the control group. Cry j 1 DNA plasmid was also injected into three atopic dogs sensitive to Cry j 1, resulting in improvement of clinical signs in the pollination season. These findings indicated that Cry j 1 DNA plasmid could regulate mast cell-mediated reaction against Cry j 1, which could be an alternative and effective treatment for CJ pollinosis. 相似文献
96.
Expression of LacZ gene in canine muscle by intramuscular inoculation of a plasmid DNA 总被引:4,自引:0,他引:4
Maeda S Ohmori K Kurata K Sakaguchi M Masuda K Ohno K Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(3):337-339
DNA immunization induces systemic humoral and cellular immune responses to the antigen encoded by cDNA in a plasmid DNA. In the present study, a plasmid DNA encoding cDNA of beta-galactosidase (beta-gal), pCAGGS-lacZ, was inoculated intramuscularly to a healthy dog in order to evaluate location and duration of the gene expression. On day 7, the plasmid DNA was found by PCR in the muscle where the plasmid was injected. Furthermore, beta-gal expression was detected in the same muscle sample by beta-gal staining. However, the plasmid DNA was not detected in any samples collected on days 14, 21 and 28. The present results suggest that duration of the gene expression of beta-gal by the plasmid DNA is limited in the muscle in dogs and an efficacy for a gene expression should be evaluated depending on the gene inserted in the plasmid DNA for immunotherapy. 相似文献
97.
Kurata K Maeda S Yasunaga S Masuda K Sakaguchi M Ohno K Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(1):25-29
Three dogs clinically diagnosed with allergic rhinitis (AR) were examined for their immunological findings. House dust mites (HDM) such as Dermatophagoides farinae (DF) and D. pteronyssinus (DP) were identified as positive allergens in the 3 dogs with both intradermal skin test and serum antigen-specific IgE test. Lymphocyte blastogenic response of peripheral blood mononuclear cells (PBMCs) under stimulation with DF antigen in dogs with AR was higher than that in 4 healthy control dogs. Expression level of IL-4 mRNA in PBMCs obtained from the 3 AR dogs was higher than that in PBMCs obtained from 4 healthy control dogs before and after stimulation with DF antigen. Expression level of IFN-gamma mRNA in PBMCs was not different between the AR and control dogs before and after stimulation with DF antigen. These results suggested that allergic reaction to HDM antigen and T(H)2-type immune response were associated with the development of AR in 3 dogs examined in this study. 相似文献
98.
99.
Shari M. Greenberg Caryn E. Plummer Dodd Sledge Misa Komine Serena L. Craft Julia A. Conway 《Veterinary ophthalmology》2016,19(4):332-339
A 1‐day–old miniature horse filly was presented to the University of Florida Veterinary Ophthalmology service for evaluation of multiple ocular anomalies which were present from birth. Protruding from the right orbit was a large, fluctuant, red, dry and variably ulcerated mass. A globe could not be appreciated clinically in the left orbit. Ocular ultrasound of both orbits was performed. This revealed a lobular, hypo‐echoic structure filling the right orbit, with multiple hyperechoic septations; normal ocular structures were not identified. Ultrasound of the left orbit revealed a microphthalmic eye, with a well‐defined, hyperechoic structure in the vitreous that was thought to be the lens. Due to irreversible blindness, the foal was humanely euthanized. Histopathology and immunohistochemistry of the orbital contents revealed bilateral microphthalmos with cyst, a congenital defect rarely reported in the veterinary literature. 相似文献
100.
YE Jing LIU Te-si JIN Xian-guo PIAO Ying-shi ZHAO Yuan-yuan HAN Jing-yi LIN Zhen-hua HARADA Kenichi REN Xiang-shan 《园艺学报》2016,32(5):886-891
AIM: To explore the effect of dual PI3K/Akt/mTOR inhibitor NVP-BEZ235 on autophagy of polycystic kidney (PCK) rat cholangiocytes. METHODS: The protein levels of p-mTOR and p-Akt in the bile duct epithelial cells were examined by immunohistochemistry. The effect of NVP-BEZ235 on the viability of cholangiocytes was detected by WST-1 assay. The levels of PI3K/Akt/mTOR signaling pathway and autophagy-related proteins with NVP-BEZ235 treatment were determined by Western blot. The effects of LC3 and Beclin 1 silencing, and authophagy-specific inhibitor 3-methyladenine (3-MA) on the cell viability were analyzed by WST-1 assay. RESULTS: The protein levels of p-Akt and p-mTOR were highly increased in the bile duct epithelium of the PCK rats. NVP-BEZ235 significantly inhibited the viability of the cholangiocytes in a dose- and time-dependent manner (P<0.05). NVP-BEZ235 significantly reduced the levels of PI3K/Akt/mTOR signaling pathway-related proteins in the PCK rat cholangiocytes. NVP-BEZ235 upregulated the autophagy-specific proteins LC3 II and Beclin 1. The inhibitory effect of NVP-BEZ235 on the cell viability was weakened by treatment with 3-MA and knockdown of LC3 and Beclin 1 (P<0.01).CONCLUSION: The PI3K/Akt/mTOR inhibitor NVP-BEZ235 suppresses the viability of PCK rat cholangiocytes, and the mechanism is closely related with autophagy. 相似文献