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91.
Akira IGUCHI Masahiro UENO Tsuneo MAEDA Takashi MINAMI Isao HAYASHI 《Fisheries Science》2004,70(4):569-572
ABSTRACT: The purpose of the present paper was to examine the genetic population structure of Buccinum tsubai in the Japan Sea. Mitochondrial 16SrRNA gene sequence analysis was performed with specimens from various stations in the Japan Sea. Mitochondrial lineages were clearly patterned geographically in four separate areas: the Hokkaido area, the Yamagata–Toyama area, the Yamato Bank area, and the San'in area. The main distribution depth range of B. tsubai is between 200 m and 1000 m isodepths, and the horizontal distance between the 200 m and 1000 m isodepth lines represents the specific spatial scale of the habitat (SSSH). These four areas were separated either by the complete discontinuity of the SSSH area or by its narrow spatial extension. Genetic distances between the main haplotypes of each area were calculated as Jukes–Cantor distances, the value of which ranged between 0.012 and 0.017. This value seemed to be unrelated to the geographic distance. There was no tendency for clustering according to depth. In future, the morphological characters of the four lineages of B. tsubai should be compared in detail in order to elucidate significant genetic differences among them. 相似文献
92.
Junko SUZUKI Yohei NISHIO Yuki KAMEO Yutaka TERADA Ryusei KUWATA Hiroshi SHIMODA Kazuo SUZUKI Ken MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1457-1463
In 2007–2008, a canine distemper virus (CDV) epidemic occurred among wild animals in
Wakayama Prefecture, Japan, and many mammals, including the wild boar and deer, were
infected. In this study, CDV prevalence among wild animals was surveyed before and after
the epidemic. At first, an enzyme-linked immunosorbent assay (ELISA) with horseradish
peroxidase-conjugated protein A/G was established to detect CDV antibodies in many
mammalian species. This established ELISA was available for testing dogs, raccoons and
raccoon dogs as well as virus-neutralization test. Next, a serological survey of wild
mammalians was conducted, and it was indicated that many wild mammalians, particularly
raccoons, were infected with CDV during the epidemic, but few were infected before and
after the epidemic. On the other hand, many raccoon dogs died during the epidemic, but CDV
remained prevalent in the remaining population, and a small epidemic occurred in raccoon
dogs in 2012–2013. These results indicated that the epidemic of 2007–2008 may have been
intensified by transmission to raccoons. 相似文献
93.
Most of the acid lakes and rivers in Japan have been influenced by acid waters of volcanic origin. Acidophilic and acidotolerant organisms are observed in these natural acid lakes and rivers, each of which has its own ecosystem and history. Lake Usoriko (pH 3.4–3.8) is a lake which.has been acidified by strongly acid water containing sulfuric acid and hydrochloric acid. The ecosystem of Lake Usoriko consists of aquatic vascular plants, aquatic bryophytes (Drepanocladus fluitans, etc.), algae, fish (Tribolodon hakonensis), insects, Zooplankton, fungi and bacteria. The paleosediment distributed along Lake Usoriko's northern shore is partly silicified and it contains fossils of a moss (Drepanocladus fluitans) and diatoms such as Pinnularia braunii var. amphicephala which are also distributed in the present lake. 14C dating of the specimens in the paleosediment, shoots of D. fluitans and the wood and cones of Picea glehnii gave values in the range 11500–34000y B.P. This record shows how long Lake Usoriko has been an acid lake. 相似文献
94.
Development of Automatic Continuous Measurement System of Chemical Constituents in the Precipitation
Yasushi Narita Kei Satoh Kenichi Hayashi Shigeru Tanaka 《Water, air, and soil pollution》2001,130(1-4):1523-1528
Nowadays, acid rain is generally noticed as a global environmental problem. While acid rain has very much to do with the air pollutants, the relation between air pollution and chemical constituents in precipitation is not understood clearly yet. It is important to measure a variation of ion concentration in precipitation in short term for understanding the formation mechanism of acid rain. Therefore, an automatic continuous measurement system of chemical constituents in precipitation was developed and put into practical use in this study. The developed system was able to collect automatically every 1mm of precipitation and analyze major ions within 20 minutes. 相似文献
95.
Antioxidative activity of green tea polyphenol in cholesterol-fed rats 总被引:17,自引:0,他引:17
This study investigated the effects of green tea polyphenol on the serum antioxidative activity and cholesterol levels of cholesterol-fed rats and compared them with those of probucol, an antioxidant hypocholesterolemic agent. To evaluate the antioxidative activity, the susceptibility to oxidative modification of low-density lipoprotein (LDL) isolated from the serum of cholesterol-fed rats was measured, as was the serum antioxidative activity using the spontaneous autoxidation system of brain homogenate. Administration of green tea polyphenol effectively inhibited LDL oxidation and elevated serum antioxidative activity to the same degree as probucol. However, higher amounts of polyphenol than probucol needed to be administered to reduce the total, free, and LDL cholesterol levels. Furthermore, green tea polyphenol increased the levels of high-density lipoprotein (HDL) cholesterol, leading to dose-dependent improvement of the atherogenic index, an effect that was not seen with probucol. Thus, green tea polyphenol may exert an antiatherosclerotic action by virtue of its antioxidant properties and by increasing HDL cholesterol levels. 相似文献
96.
Mst. Parvin MOSTARI Nahoko IEDA Chikaya DEURA Shiori MINABE Shunji YAMADA Yoshihisa UENOYAMA Kei-ichiro MAEDA Hiroko TSUKAMURA 《The Journal of reproduction and development》2013,59(3):266-272
Accumulating evidence suggests that the arcuate nucleus (ARC) kisspeptin/neurokinin B
(NKB)/dynorphin (KNDy) neurons play a role in estrogen negative feedback action on
pulsatile gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) release. The
present study aimed to determine if dynorphin (Dyn) is involved in estrogen negative
feedback on pulsatile GnRH/LH release. The effect of the injection of nor-binaltorphimine
(nor-BNI), a kappa-opioid receptor (KOR) antagonist, into the third cerebroventricle (3V)
on LH pulses was determined in ovariectomized (OVX) adult female rats with/without
replacement of negative feedback levels of estradiol (low E2). The mean LH
concentrations and baseline levels of LH secretion in nor-BNI-injected, low
E2-treated rats were significantly higher compared with vehicle-treated
controls. On the other hand, the nor-BNI treatment failed to affect any LH pulse
parameters in OVX rats without low E2 treatment. These results suggest that Dyn
is involved in the estrogen negative feedback regulation of pulsatile GnRH/LH release. The
low E2 treatment had no significant effect on the numbers of ARC
Pdyn (Dyn gene)-,Kiss1- and
Tac2 (NKB gene)-expressing cells. The treatment also did not affect
mRNA levels of Pdyn and Oprk1 (KOR gene) in the
ARC-median eminence region, but significantly increased the ARC kisspeptin
immunoreactivity. These findings suggest that the negative feedback level of estrogen
suppresses kisspeptin release from the ARC KNDy neurons through an unknown mechanism
without affecting the Dyn and KOR expressions in the ARC. Taken together, the present
result suggests that Dyn-KOR signaling is a part of estrogen negative feedback action on
GnRH/LH pulses by reducing the kisspeptin release in female rats. 相似文献
97.
Tsuji T Kanai K Yokoyama A Tamura T Hanamure K Sasaki K Takata R Yoshida S 《Journal of agricultural and food chemistry》2012,60(24):6197-6203
"Fishy aftertaste" is sometimes perceived in wine consumed with seafood. Iron in wine has been reported to be a key compound that produces fishy aftertaste. However, cost-effective methods to remove iron from wine have not been developed. Here, we describe a cost-effective and safe iron adsorbent consisting of alcohol-treated yeast (ATY) cells based on the observation that nonviable cells adsorbed iron after completion of fermentation. Treatment of cells with more than 40% (v/v) ethanol killed them without compromising their ability to adsorb iron. Drying the ATY cells did not reduce iron adsorption. Use of ATY cells together with phytic acid had a synergistic effect on iron removal. We term this means of removing iron the "ATY-PA" method. Sensory analysis indicated that fishy aftertaste in wine-seafood pairings was not perceived if the wine had been pretreated with both ATY cells and phytic acid. 相似文献
98.
Different thermotolerances in in vitro‐produced embryos derived from different maternal and paternal genetic backgrounds
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Miki Sakatani Kenichi Yamanaka Ahmed Zaky Balboula Masashi Takahashi 《Animal Science Journal》2017,88(12):1934-1942
The present study evaluated the effects of genetic backgrounds on the developmental competence and thermotolerance of bovine in vitro‐produced (IVP) embryos. First, Holstein (Hol) and Japanese Black (JB) oocytes were fertilized with sperm from Hol, JB and a thermotolerant breed (Brahman), and in vitro development was evaluated when the embryos were exposed to heat shock on Day 2 (Day 0 = day of fertilization). Sperm genetic backgrounds affected the developmental competence in controls (P < 0.05). Second, the effect of sperm pre‐incubation for 4 h on subsequent in vitro fertilization was assessed using different sperm genetic backgrounds. The pre‐incubation of sperm did not decrease the embryonic development regardless of the breed of the sperm. A milder heat shock (40.0°C) effect on parthenotes (Hol and JB) and IVP embryos were evaluated. JB parthenotes showed developmental arrest after Day 4, and the rate of development to the blastocyst stage decreased by heat shock, but not in Hol parthenotes. Heat shock decreased developmental competence after cleavage of IVP embryos regardless of genetic background. The thermotolerance of IVP embryos would be controlled by both maternal and paternal factors but genetic involvement was still unclear. Further evaluation is needed to reveal the genetic contribution to thermotolerance. 相似文献
99.
Correlation between the proportion of stained eggs and the number of mites (Dermanyssus gallinae) monitored using a ‘non‐parallel board trap’
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Makiko Odaka Kazumasa Ogino Michitaka Shikada Kenichi Asada Syoujirou Kasa Takahiro Inoue Ken Maeda 《Animal Science Journal》2017,88(12):2077-2083
The poultry red mite (Dermanyssus gallinae) is a serious problem for the poultry industry worldwide. However, the relationship between the mite population and the damage that they cause is still unclear. In this study, the mite population in poultry houses was examined using an established trap method, and the risk of blood‐stained eggs caused by the mites was assessed. Traps were placed once a week outside the egg channels and/or on the floor in two poultry farms in Fukuoka Prefecture, Japan, from April 2012 to July 2014. The numbers of blood‐stained eggs and total eggs were counted at weekly intervals. The results showed that the number of mites increased from April to May, and reached a peak around the beginning of June when the average temperature and humidity were >24°C and 70–90%, respectively. In the segmented model, the correlation between the proportion of blood‐stained eggs and the number of mites or temperature was positive over a threshold. In conclusion, our established trap method is useful for monitoring mites and can be used to predict when poultry farms should be treated to prevent appearance of blood‐stained eggs. 相似文献