Immunoblot analysis for IgE-reactive components of fetal calf serum in dogs that developed allergic reactions after non-rabies vaccination     

Ohmori K  Masuda K  DeBoer DJ  Sakaguchi M  Tsujimoto H 《Veterinary immunology and immunopathology》2007,115(1-2):166-171

We previously demonstrated the presence of IgE directed to fetal calf serum (FCS) in the sera from dogs that developed allergic reactions after vaccination. In this study, by an immunoblot analysis, we investigated the IgE-reactive components of FCS using sera from 16 dogs that exhibited allergic reactions after vaccination. The immunoblot analysis revealed that several FCS proteins of approximately 25-, 50-, 66-, 75-, 120-, and 175-kDa strongly reacted with IgE in the sera from dogs that showed post-vaccination allergic reactions. The 66-kDa band was detected in the sera from 14 of the 16 dog serum samples analyzed in the immunoblot analysis for FCS, and it was speculated to be albumin based on its molecular weight; however, serum IgE reactivity to bovine serum albumin could be detected in only four of the 14 dog samples. These findings demonstrated that a variety of FCS components including albumin could function as allergens in dogs that developed allergic reactions after vaccination.  相似文献   

Food allergens inducing a lymphocyte-mediated immunological reaction in canine atopic-like dermatitis     

Akemi SUTO  Yukinori SUTO  Nozomi ONOHARA  Yu TOMIZAWA  Yukiko YAMAMOTO-SUGAWARA  Taro OKAYAMA  Kenichi MASUDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):251-254

Canine atopic-like dermatitis (ALD) is suspected to be associated with food allergies, particularly those mediated by lymphocytes. In this study, 54 cases were included as ALD dogs, based on the negative IgE test results. In the dogs, the percentage of activated cells in helper-T lymphocytes was measured by flow cytometry using cultured peripheral lymphocytes under food allergen stimulation. We observed that 49 of the 54 ALD dogs (90.7%) had positive lymphocyte reactions against one or more food allergens. The most common food allergen was soybean, showing positive results in 21 dogs (42.9%), while the allergen to cause the lowest number of reactions was catfish (only 5 dogs, 10.2%). These results may be useful in considering elimination diets for ALD dogs.  相似文献   

Prevalence of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in piglets after weaning on a commercial pig farm in Japan     

Kenichi SASAKI  Takamitsu TSUKAHARA  Osamu TAIRA  Kotaro TSUCHIYA  Mitsugi ITOH  Kazunari USHIDA 《Animal Science Journal》2010,81(1):135-141

To investigate the transition in concentration of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) and antibody for these viruses in serum, serum samples were collected from 29 pigs on weaning day and at 7, 14, 21, 28, 53, 84, and 120 days after weaning. The concentration of circulated PRRSV and PCV2 in serum was measured by real-time RT-PCR and real-time PCR, respectively. The specific antibody for PRRSV and PCV2 was measured using ELISA. PRRSV was not detected on 0 days post-weaning (dpw). The specific antibody for PRRSV began to increase as the concentration of PRRSV in serum increased, and the level of PRRSV then tended to decrease. PCV2 was detected in 12 of 28 pigs on 0 dpw. The concentration of PCV2 and the specific antibody for PCV2 showed a similar tendency to those of PRRSV. The correlation analysis suggests that a decline in the daily weight gain coincided with an increase in the PRRSV concentration. Pigs with a higher antibody titer against PRRSV or PCV2 on 0 dpw showed the lower level of PRRSV or PCV2, respectively.  相似文献   

Genetic diversity and expression analysis of granule bound starch synthase I gene in the new world grain amaranth (Amaranthus cruentus L.)     

Young-Jun Park  Kazuhiro Nemoto  Tomotaro Nishikawa  Kenichi Matsushima  Mineo Minami  Makoto Kawase 《Journal of Cereal Science》2011

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Production and properties of Japanese oriented strand board I: effect of strand length and orientation on strength properties of sugi oriented strand board     

Shigehiko Suzuki  Kenichi Takeda 《Journal of Wood Science》2000,46(4):289-295

Oriented strand boards (OSB) were made using sugi wood strand with different lengths at different free fall distance conditions. Strand alignment and mechanical properties of sugi OSB were evaluated. Results obtained can be summarized as follows. The alignment angle distribution was greatly affected by both free fall distance and strand length. It was found that the standard deviation of the angles can be a measure for predicting the distribution when employing the von Mises distribution function with concentration parameter. The Monte Carlo simulation showed an agreement between the theoretical considerations and the experimental results on the strand alignment. The mechanical properties as affected by both strand length and layer structure were determined. Bending properties could be equal in both directions at 25% face layer ratio. Young's modulus obtained by the in-plane vibration method showed almost linear relation to the face layer ratio. No significant differences or only a slight difference was observed for the internal bond strength, plate-shear modulus, and nail resistance properties. Further studies are necessary.  相似文献   

Genetic diversity of Ralstonia solanacearum and disease management strategy     

Kenichi Tsuchiya 《Journal of General Plant Pathology》2014,80(6):504-509

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Pathology of female mice experimentally infected with an in vitro cultured strain of Trypanosoma equiperdum     

Yusuke TANAKA  Keisuke SUGANUMA  Kenichi WATANABE  Yoshiyasu KOBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1212

Dourine, caused by infection with Trypanosoma equiperdum, is one of the trypanosomiasis in equids. The clinical course of dourine is long-term, ranging from 1–2 months to several years. Since the pathogenesis of dourine has not yet been elucidated, experimental studies using mouse infection models are needed. Although mice are not susceptible to most T. equiperdum strains, some strains can infect mice. Even in such strains, infected mice develop rapidly transient parasitemia and die within 2–8 days. Therefore, mice experimentally infected with these T. equiperdum strains are not suitable for mouse infection models to analysis the pathogenesis of dourine. A sequential method of isolating parasites from dourine-affected horses and adapting them to in vitro cultures using soft agarose media was recently developed. Various T. equiperdum strains adapted to in vitro conditions have been established using this technique. We used one of these strains, the T. equiperdum IVM-t2 strain. In the present study, T. equiperdum IVM-t2 strain inoculated mice developed periodic parasitemia during the experimental period of 60 days. Histopathologically, vaginitis and dermatitis were observed. These findings were comparable to those of dourine-affected horses. Therefore, mice infected with T. equiperdum IVM-t2 strain may be a valuable tool for pathological, immunological, and parasitological in vivo research, and will contribute to investigations on the mechanisms underlying the disease process and the host-parasite relationship.  相似文献   

Field method to monitor the mycoparasitic fungus Coniothyrium minitans     

Ryo Shukunami  Yutaka Iwamoto  Shinji Sugiura  Kentaro Ikeda  Hitohsi Nakayashiki  Kenichi Ikeda 《Journal of General Plant Pathology》2016,82(1):51-56

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The 100-year history of the Phytopathological Society of Japan     

Kenichi Tsuchiya 《Journal of General Plant Pathology》2015,81(6):483-486

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Henneguya pseudorhinogobii n. sp. (Myxozoa: Myxosporea) parasitizing the gills of the freshwater goby Rhinogobius sp. OR from the Nagara River and redescription of Henneguyarhinogobii     

Tetsushi Kageyama  Tetsuya Yanagida  Kenichi Ohara  Hiroshi Yokoyama 《Fisheries Science》2009,75(3):657-663

Two types of infection with myxosporean parasites, which were different in cyst size, were found from the gill of Rhinogobius sp. OR collected from the Nagara River, Gifu Prefecture, Japan. A myxosporean forming large-type cysts in the gill filament was morphologically identified as Henneguya rhinogobii. Another myxosporean forming small-type cysts was found to be parasitic in the gill lamella. Average spore sizes of the latter myxosporean were 15.8 (range 14.2–17.8) μm in length, 5.3 (4.7–5.8) μm in width, 6.5 (5.9–7.6) μm in polar capsule length and 34.9 (25.3–42.9) μm in caudal appendage length, which were almost identical to those of H. rhinogobii. However, the two were distinguishable by the location in the gill (intrafilamental or intralamellar) and by the difference in the sequence of SSU rDNA (about 96% similarity). We propose the myxosporean forming intralamellar small-type cysts as Henneguya pseudorhinogobii n. sp. and redescribe H. rhinogobii by morphological and molecular studies.  相似文献