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991.
Wood formation of trees that grow along the seasons has an annual rhythm. Due to this rhythm, physiological research on the mechanism of wood formation has been difficult to conduct in a typical experimental room. In the present study, we observed the wood tissue formation in a shortened annual cycle system, which was developed for poplar trees grown in a growth chamber with dormant and non-dormant cycles. Poplar trees were grown in this system by repeating the cycle three times. The resulting wood tissue consisted of three growth rings and very similar structures were observed around the ring boundary of the wood in a field-grown stem. This result suggests that the shortened annual cycle system can be adopted as a model for physiological, cell biological and molecular research of wood and annual ring formation. 相似文献
992.
Chimeric mice with humanized liver as a model for testing organophosphate and carbamate pesticide exposure
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Hiroshi Suemizu Kenji Kawai Norie Murayama Masato Nakamura Hiroshi Yamazaki 《Pest management science》2018,74(6):1424-1430
BACKGROUND
Diagnosis of acute intoxication with organophosphate (OP) or carbamate (CM) pesticides in humans is achieved by measuring plasma butyrylcholinesterase (BuChE) activity. However, BuChE activity is not an ideal biomarker in experimental animal models. The aim of this study was to establish an experimental mouse model for evaluating exposure to OP and CM pesticides by monitoring BuChE activity using chimeric mice in which the liver was reconstituted with human hepatocytes.RESULTS
A single oral administration of acephate (300 mg/kg), chlorpyrifos (10 mg/kg), fenobucarb (300 mg/kg) or molinate (250 mg/kg) in chimeric mice led to inhibition of >95%, > 95%, 28% and 60% of plasma BuChE activity after 7, 0.5, 0.5 and 7 h, respectively. Dose‐dependent decreases in plasma BuChE activity were also observed for acephate and chlorpyrifos. A 5‐day repeated‐dose study with 10 or 30 mg/kg acephate found a constitutive reduction in plasma BuChE activity to 80% and 70% of pre‐dose levels, respectively.CONCLUSION
Changes in plasma BuChE activity in chimeric mice with humanized liver clearly reflected the exposure levels of OP and CM pesticides. These results suggest that the humanized‐liver mouse model may be suitable for estimating levels of exposure to these pesticides in humans. © 2017 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献993.
Kenji Ono Akane Komoriya Ryuichi Tachibana Akihiro Imaya Satoru Suzuki Hironori Noguchi 《Soil Science and Plant Nutrition》2018,64(2):168-180
ABSTRACT To successfully afforest coastal forest belts ensuring high disaster prevention, growth bases for them have been constructed by piling up soil in the low wetlands along the Kujukuri coastline. Ground surfaces in such bases are often covered with water because of soil compaction, leaving them susceptible to stagnant water. Water stagnation in soil is problematic, potentially interfering with afforesting coastal forests. Therefore, row deep tillage was conducted for parts of growth bases to combat the poor physical properties of the existing soil. Here, we surveyed soil profiles and measured vertical soil hardness distribution in two forest stands with piling up soil to evaluate the effectiveness of tillage for man-made soil. Soil hardness measurements indicated that the vertical areas with ‘soft’ and ‘hard/consolidated’ soil alternately appeared in growth base profiles. Generally, soil of the dense and very hard layers was apt to be formed by strong compaction of the filled-up soil because of heavy machinery usage during growth base preparation. Such dense and hard soils in the untilled areas of the profiles were also observed in this study. By contrast, it was confirmed that row deep tillage drastically improved soil physical properties, i.e., decreased hardness and increased water permeability, because the sequential hardened subsoil layers were well-broken-up. Moreover, it was observed many thick and large roots penetrated deeper layers with deep tillage areas. These results suggest that row deep tillage of hardened soil is quite effective at securing the areas and providing the physical conditions for deeply penetrated roots into deeper soils, which increases healthy root development. They show the effects of soil hardness reduction and water permeability improvement have been maintained for two decades, at least, after construction. These findings will be useful for alleviating some problems of soil compaction, water stagnation, and tree-growth hindrance that have been encountered on afforestation sites with man-made soil. 相似文献
994.
Kenji Ishihara Chiaki Oyamada Yoko Sato Toshiyuki Suzuki Masaki Kaneniwa Hiromi Kunitake Toshihiko Muraoka 《Fisheries Science》2010,76(6):1015-1021
Glycerol galactoside (GG; floridoside: 2-O-glycerol-α-d-galactopyranoside, and isofloridoside: 1-O-glycerol-α-d-galactopyranoside) is known to be a component of edible red seaweed nori. Recently, we have found that low-quality nori (color-faded
nori), which has a low protein content, contains a large quantity of GG. From further studies, we have also found that GG
has prebiotic characteristics in vitro. In this study, we evaluated the in vivo prebiotic activity of GG in rats. Dietary
GG selectively increased the cecal Bifidobacterium count in rats. Other indices of prebiotics, such as pH of cecal content, organic acid concentrations, and fecal weight, also
supported the existence of prebiotic activity of GG. The present data will also contribute to the development of a new method
of utilizing color-faded nori as a health-promoting foodstuff. 相似文献
995.
Upgrading of urea formaldehyde-bonded reed and wheat straw particleboards using silane coupling agents 总被引:3,自引:0,他引:3
Guangping Han Changwu Zhang Dongmei Zhang Kenji Umemura Shuichi Kawai 《Journal of Wood Science》1998,44(4):282-286
Reed and wheat straw particleboards bonded with urea formaldehyde (UF) resin were manufactured from two different material configurations (i.e., fine and coarse particles). The board densities were in the range of 0.550–.90g/cm3. The effects of particle size and board density on the board properties were examined. The properties of particleboard produced from fine particles were better than those made from coarse particles. An increase in board density resulted in a corresponding improvement in the board properties. The properties of OF bonded reed and wheat straw particleboards were relatively lower than those of commercial particleboards. Three silane coupling agents were used to improve the bondability between the reed and wheat particles and OF resin. Results of this study indicate that all the board properties were improved by the addition of silane coupling agent. The degree of improvement achieved from each coupling agent was different; epoxide silane was found to be more effective for reed straw particleboard, and amino silane was better for wheat straw particleboard. 相似文献
996.
Ichiro?Nagano Seiko?Inoue Kenji?Kawai Syun-ichirou?OshimaEmail author 《Fisheries Science》2009,75(3):707-714
Pseudotuberculosis is a bacterial septicaemia caused by Photobacterium damselae subsp. piscicida in several marine fish species. Yellowtail Seriola quinqueradiata is the most sensitive fish species to this disease. The internal organs of naturally infected yellowtail exhibit whitish
spots, tubercle-like tissue structures, consisting of bacterial accumulations. There have been many trials for experimental
infection, however adequate method of infection that reproduces moderate mortality and primary clinical signs has not yet
established. Present investigation evaluated an immersion infection method by using logarithmic culture-phase bacteria resulting
in higher mortality than that using stationary culture-phase bacteria. Typical white spots on the spleen and kidney were also
observed constantly in dead fish. Transmission electron microscopy and fluorescent antibody microscopy showed bacterial clusters
not only in the spleen and kidney but also in the blood channels in the secondary gill filaments. These results were confirmed
repeatedly by plural experiments. The use of logarithmic-phase bacteria in immersion infection is an appropriate technique
to reproduce moderate mortality and primary clinical signs, which will be a reliable infection method also for the challenge
test of pseudotuberculosis vaccine. 相似文献
997.
Seung-Cheol Ji Osamu Takaoka Si-Woo Lee Jae-Ho Hwang Yang-Su Kim Katsuya Ishimaru Manabu Seoka Gwan-Sik Jeong Kenji Takii 《Fisheries Science》2009,75(3):665-672
The effect of dietary medicinal herbs on lipid metabolism and stress recovery was investigated in red sea bream Pagrus major. Fish (mean body weight 24.0 ± 0.2 g) were fed on test fish meal diets supplemented with either Massa Medicata (Mm), Crataegi
Fructus (Cf), Artemisia capillaries (Ac), or Cnidium officinale (Co), or with a mixture of the four herbs (HM) for 12 weeks. A control group was fed a diet without herbs in the same manner.
A high survival rate was observed in the herbal diet groups. The final mean body weight, feed efficiency, protein efficiency
ratio, and apparent protein and lipid retention in the Ac, Co, and HM groups were higher than those in the control and Mm
groups. Final carcass, hepatic lipid and triglyceride contents, and plasma triglyceride and nonesterified fatty acid levels
were lower in the Ac, Co, and HM groups compared to those of control and Mm groups. However, final hepatic phospholipid, plasma
phospholipid, and high density lipoprotein cholesterol levels were higher in the Ac, Co, and HM diets groups than in the control
and Mm groups. The Cf, Co, Ac, and HM groups showed faster recovery time in the 2-phenoxyethanol anesthesia test and a higher
recovery rate in the 10-min air exposure test than those of the control and Mm groups. Moreover, the Cf, Ac, Co, and HM diet
groups had a significantly lower plasma cortisol level than the control and Mm diet group, but the glucose level in the herbal
diet groups was higher than that in the control group after a 1-h air exposure. These results indicate that the addition of
medicinal herbs to the fish diet improved lipid utilization and stress recovery in red sea bream. 相似文献
998.
Kenji Fujino Mari Obara Hitoshi Kiuchi Hiroshi Shinada Tsutomu Nishimura Toshihiko Maekawa Yuji Hirayama 《Plant Breeding》2020,139(5):845-852
A series of DNA markers for various agronomic traits may accelerate the success of marker-assisted selection in practical plant breeding programmes. Here, we developed DNA markers for the blast resistance gene Pi-cd. In this study, we examined the effects of the Pi-cd locus on not only blast resistance but also agronomic traits in agriculture. We developed three pyramiding lines (PLs) coupling Pi-cd with three blast resistance genes, pi21, Pi35 and Pi39. The effect of Pi-cd on blast resistance was dependent on the coupled resistance genes. Then, we evaluated the effects of Pi-cd on 13 agronomic traits. Amylose content and 1,000-grain weight showed significant differences between the PLs and current commercial varieties, which had no negative effects on agronomic trait values. Furthermore, we investigated the distribution of genotype for the Pi-cd locus among varieties of upland rice. The KT genotype specific to rice blast resistance may be predominant in the varieties. The results suggested that Pi-cd has the potential to be useful for improving blast resistance in rice breeding programmes. 相似文献
999.
Pre‐challenge and post‐challenge haemato‐immunological changes in Oreochromis niloticus (Linnaeus, 1758) fed argan oil against Lactococcus garvieae
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Esin Baba Ümit Acar Sevdan Yılmaz Canan Öntaş Osman Sabri Kesbiç 《Aquaculture Research》2017,48(8):4563-4572
The present study investigated the effects of argan oil, obtained from Argania spinosa, on pre‐ and post‐challenge immuno‐haematological and biochemical responses of Nile tilapia, Oreochromis niloticus. For this purpose, the fish were fed diets containing 0, 0.5%, 1% or 2% argan oil for 45 days. Following 45 days of feeding, fish were challenged with Lactococcus garvieae and mortality was recorded for 15 days. During the pre‐challenge period, significantly higher respiratory burst activity, total white blood cell (WBC), serum lysozyme activity and myeloperoxidase activity were determined in the argan oil‐fed groups. The serum glucose and cholesterol levels decreased whilst total protein and albumin did not change in the groups fed with argan oil‐supplemented diets. After challenge with Lactococcus garvieae, the percentage survival (%) was found to be the highest in the 1% and 2% argan oil‐supplemented feeding groups. Also, there was a significant increase in weight gain, specific growth rate and feed conversion ratio in those fish fed argan oil. The results of this study indicated that after the supplementation of fish diets with argan oil, especially at 1% and 2% concentrations, the immunological, haematological and biochemical values remained similar in both the pre‐ and post‐challenge periods and the immune response against L. garvieae in Nile tilapia was modulated. 相似文献
1000.
Xiaoqiang Li Chen Shi Jidong Wang Jian Wang Mengjuan Li Hua Qiu Hong Sun Kenji Ogino 《Fibers and Polymers》2017,18(1):50-56
A simple and practical strategy has been developed for preparing polyaniline(PANi)-doped TiO2/poly(l-lactide) (P@TiP-C) fibers by a combination of coaxial-electrospinning and in-situ polymerization. The TiO2/PLLA composite fibers with TiO2 located on the surface were fabricated by coaxial-electrospinning, with PLLA as the core phase and a dispersion of TiO2 particles, a well-known photocatalyst, in the sheath phase. The aniline monomers were also located in the core phase and in-situ polymerized by ammonium persulfate (APS) after electrospinning. SEM images show that TiO2 particles were located on the surface of PLLA fibers. Photocatalytic degradation tests show that the P@TiP-C fibers exhibit enhanced photocatalytic activity for degradation of methyl orange under visible light, likely due to the synergistic effect of PANi and TiO2. 相似文献