Visceral urate deposits in chicks inoculated with avian nephritis virus     

J Shirai  K Nakamura  M Narita  K Furuta  H Hihara  H Kawamura 《The Veterinary record》1989,124(25):658-661

Avian nephritis virus, G-4260 strain, was inoculated orally into one-day-old specific-pathogen-free chicks of two lines. Approximately 20 per cent of the chicks of both lines died with visceral urate deposits from eight to 12 days after infection, and the virus was isolated from the kidneys of the dead chicks. At 14 or 15 days of age the mean liveweight of the surviving infected chicks was approximately 16 per cent less than that of the uninfected control chicks.  相似文献   

Determination of canine beta2-microgloblin in plasma and urine by enzyme-linked immunosorbent assay     

Nakajima Y  Hoshi F  Higuchi S  Kawamura S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):343-345

An enzyme-linked immunosorbent assay was developed for the determination of canine beta2-microglobulin (beta2-m) in plasma and urine. The detectable sensitivity for pure canine beta2-m was 0.05 microg/l and the analytical range was 0.1 to 50 microg/l. The mean analytical recovery when pure canine beta2-m was added to normal plasma was 101.9%. The mean analytical recovery in the urine was 102.1%. The intra-day variation coefficient was 3.1% in plasma, 4.3% in serum and 1.9% in urine. No difference was found between the concentration of beta2-m in plasma and serum (n=17). The concentration of beta2-m in the plasma of normal dogs was 1.82 +/- 0.57 mg/l (n=31). The mean excretion in 24 hr urine collected from normal dogs was 17.6 +/- 9.2 microg/l, 0.22 +/- 0.12 microg/kg of body weight or 14.2 +/- 9.4 microg/g of urine creatinine. The beta2-m creatinine index of random urine samples was 23.5 +/- 16.6 microg/g (n=26). There was a close correlation between the beta2-m creatinine index of 24 hr urine samples and that of random urine samples (r=0.872).  相似文献   

Reduced Efficacy of Ivermectin Treatments in Gastrointestinal Nematode Infections of Grazing Cattle in Japan     

Noboru KUDO  Toshiro YOSHIOKA  Yasushi WATANABE  Yuki TERAZONO  Shino TAKENOUCHI  Takuto DONOMOTO  Kensuke NAKAJIMA  Kaori HITOSUGI  Ryusuke TSUKADA  Hiromi IKADAI  Takashi OYAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(11):1487-1491

  相似文献   

Variability of Micro-elevation,Yield, and Protein Content within a Transplanted Paddy Field     

K.?ShojiEmail author  T.?Kawamura  H.?Horio  K.?Nakayama  N.?Kobayashi 《Precision Agriculture》2005,6(1):73-86

Due to the nature of waterlogged fields used for rice production, we hypothesized that micro-elevation (micro-relief, micro-topography, or differences in elevation) is an important factor for site-specific management within rice fields. A 0.5-ha transplanted and weed-free paddy field was selected as the observation site, where there was micro-elevation in a range of 100 mm within the field. Combine-monitored grain yield and the surveyed micro-elevation were compared at 96 locations in the field, and 60 hand-taken grain samples were analysed for protein content. Grain yield and protein content showed significant negative correlations with micro-elevation (r=-0.50^*** and -0.67^***, respectively), indicating that at lower elevations, grain yield increased gradually with protein content. Spatial variation in yield and protein content was attributed to availability of water and nutrient uptake at locations with different micro-elevation. Therefore, micro-elevation is expected to be one of the important factors for managing spatial variation in a small paddy field.  相似文献   

Urinary free metanephrines measurement in dogs with adrenal gland diseases using a new simple liquid chromatography tandem mass spectrometry method     

Noboru SASAKI  Yoshinori IKENAKA  Yumiko INOUE  Takahiro ICHISE  Noriyuki NAGATA  Mayumi ISHIZUKA  Syouta MM NAKAYAMA  Kensuke NAKAMURA  Mitsuyoshi TAKIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):648

Measurement of urinary metanephrines in spot samples is used for the diagnosis of canine pheochromocytoma (PC). We describe a simple analytical method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for measuring free metanephrine (MN) and normetanephrine (NMN) in spot urine samples. Using the developed method, we evaluated the stability of urinary free-MN and free-NMN at various storing conditions. In addition, we assessed the feasibility of urinary free-MN and -NMN measurement for diagnosing PC. Urine samples were mixed with stable isotope internal standards and thereafter purified by ultrafiltration. The purified samples were analyzed by LC-MS/MS in the multiple reaction monitoring mode after separation on a multimode octa decyl silyl column. The coefficient of variation of free-MN and -NMN measurement was 7.6% and 5.5%, respectively. The linearity range was 0.5–10 µg/l for both analytes. Degradation was less than 10% for both analytes under any of the storage conditions. The median free-NMN ratio to creatinine of 9 PC dogs (595, range 144–47,961) was significantly higher (P<0.05) than that of 13 dogs with hypercortisolism (125, range 52–224) or 15 healthy dogs (85, range 50–117). The developed method is simple and may not require acidification of spot urine. The results of this preliminary retrospective study suggest that the measurement of urinary free metanephrines is a promising tool for diagnosing canine PC.  相似文献   

Screening method for determination of high levels of cadmium, lead, and copper in foods by polarized Zeeman atomic absorption spectrometry using discrete nebulization technique     

I Saito  H Oshima  N Kawamura  M Yamada 《Journal of the Association of Official Analytical Chemists》1988,71(4):829-832

A screening method for determination of cadmium, lead, and copper in foods was developed. The sample (1-3 g) is digested with HNO3-H2SO4-HClO4 in a centrifuge tube attached to a straight glass tube that prevents loss of HNO3 by volatilization. After digestion, potassium iodide, H2SO4, and MIBK (4-methyl 2-pentanone) are added, and the metals are extracted with MIBK as metal iodides. The MIBK solution is injected and the metals are determined by flame polarized Zeeman atomic absorption spectrometry using a discrete nebulization technique. Recoveries of metals from fortified milk powder, unpolished rice, fish, beef, peanut butter, apple, and cabbage were satisfactory. The analytical results for NBS Oyster Tissue and NIES Pepperbush, Chlorella, and Mussel agreed with certified or reference values except lead in Pepperbush. The limits of quantitation for cadmium, lead, and copper were 0.01, 0.09, and 0.02 ppm, respectively. This method is simple and safe for routine analysis of high levels of cadmium, lead, and copper in foods.  相似文献   

Plasma Membrane Lipids Are the Powerful Components for Early Stage Aluminum Tolerance in Triticale   总被引：1，自引：0，他引：1  

Tadao Wagatsuma  Satoru Ishikawa  Matsuo Uemura  Wataru Mitsuhashi  Takeshi Kawamura  Md.  Shahadat Hossain Khan  Keitarou Tawaraya 《Soil Science and Plant Nutrition》2005,51(5):701-704

The order of aluminum (Al) tolerance in triticale lines (ST2>ST22) after re-elongation in an Al-free 0.2 mM calcium (Ca) solution for 9 h (Ca period) following 1 h pretreatment with 20 μM Al (Al period) agreed with that after 24 h of Al treatment. Permeability of the plasma membrane (PM) of root-tip cells after the Ca period was significantly increased in Al-sensitive ST22. Al was accumulated more heavily in the root-tip portion of ST22 than in that of ST2, although similar amounts of malic and citric acid anions were released from both triticale lines. We established a new system examining lipid permeability using synthesized nylon-2,8 ultrathin and porous capsules trapped previously with 0.1% (w/v) methylene blue solution and coated thereafter with PM lipid isolated from root tips by a newly developed technique. Permeability of the PM lipid measured with time in 0.2 mM Ca with or without 50 μM Al photometrically ( A ₆₈₀) was significantly greater in Al-sensitive ST22 after 5 min of Al treatment. This is the first report to directly show the primary and early role of PM lipid in Al tolerance in triticale.  相似文献   

Limited survey of residual tetracyclines in tissues collected from diseased animals in Aichi Prefecture, Japan.     

H Oka  Y Ikai  N Kawamura  J Hayakawa 《Journal of the Association of Official Analytical Chemists》1991,74(6):894-896

Tissues were collected to survey the actual conditions of tetracycline antibiotics (TCs) residues in slaughtered animals that did not pass inspection at slaughterhouses in Aichi Prefecture, Japan, because of the presence of disease symptoms. Tissues were analyzed by liquid chromatography. Among 271 samples, 49 (18.1%) were positive for oxytetracycline (OTC), 5 (1.8%) for chlortetracycline (CTC), and 5 (1.8%) for doxycycline (DC), respectively. One sample (cattle kidney) was positive for both OTC and DC. However, tetracycline was not detected in any samples. Percentage frequencies of TCs residues were 29.1% (37/127) and 15.2% (22/144) for cattle and hogs, respectively. Kidney samples showed higher incidence of TCs residues and 1.5-7 times higher residual concentrations than liver and miscellaneous samples.  相似文献   

The effect of cortisol administration on blood plasma immunoglobulin M (IgM) concentrations in masu salmon (Oncorhynchus masou)     

Masaki Nagae  Hirotoshi Fuda  Kazuhiro Ura  Hiroshi Kawamura  Shinji Adachi  Akihiko Hara  Kohei Yamauchi 《Fish physiology and biochemistry》1994,13(1):41-48

Immunoglobulin M (IgM) is known as a main factor in the humoral immune system of teleosts. In the present study, the effect of cortisol on plasma IgM concentrations was investigated using a specific antibody to IgM in masu salmon (Oncorhynchus masou). Cortisol was orally administered each day for 2 weeks at a dose of 1 mg g⁻¹ in the diet, and for the following week the fish were fed a non-treated diet. Blood plasma samples were collected at 0, 1, 2 and 3 weeks after the initiation of treatment. Oral administration of cortisol elevated plasma cortisol concentrations to about 40 ng/ml for 2 weeks after administration and slightly reduced plasma IgM concentration; the suppression was statistically significant one week after the period of hormone administration. However, treatment with cortisol did not affect plasma concentrations of total protein or α1-protein, one of the major serum proteins, during the experimental period. These results indicate that cortisol specifically suppresses plasma IgM concentrations.  相似文献   

Radula development in abalone Haliotis discus hannai from larva to adult in relation to feeding transitions   总被引：1，自引：0，他引：1  

Tomohiko Kawamura  Rodney D Roberts  Yoh Yamashita 《Fisheries Science》2001,67(4):596-605

ABSTRACT: The radula morphology of Haliotis discus hannai was examined by scanning electron microscope from the larval to the adult stage. The radula of competent larvae contained 11–13 transverse rows of teeth after 6–7 days at 20°C. The number of rows increased to 25–30 during the first several days after settlement, but then remained approximately constant throughout the post-larval period, increasing again in abalone larger than 4 mm in shell length (SL). In post-larvae < ~1 mm SL, only two pairs of lateral teeth (L1, L2) were present in the larval radula. An additional three pairs of lateral teeth (L3–L5) were added progressively as post-larvae grew from 0.9 mm to 1.9 mm SL. Marginal teeth were added steadily from one pair in larvae to 30–40 pairs at 3–4 mm SL, 70–80 pairs in 30–40 mm juveniles, and 70–90 pairs in 90–100 mm adults. The serrations on the working edges of the rachidian (R) and lateral teeth became less pronounced as the abalone grew. Nearly all serrations disappeared from the rachidian (R) and inner lateral teeth (L1, L2) by ~2 mm SL, and from the outer lateral teeth (L3–L5) by 20 mm SL. For abalone larger than 1.5 mm SL, the L3–L5 teeth became longer and more pointed, which increased the space between adjacent rows of teeth. Post-larvae < 1 mm SL had highly curved teeth with clearance angles of approximately or less than zero, whereas larger abalone had positive clearance angles. These radula developments appear to be related to transitions in feeding habits from microbial to macroalgal diets.  相似文献