F-wave conduction velocity, persistence, and amplitude for the tibial nerve in clinically normal cats   总被引：9，自引：0，他引：9  

Okuno S  Kobayashi T  Orito K 《American journal of veterinary research》2008,69(2):261-264

OBJECTIVE: To establish a method of F-wave evaluation and to determine normative values of F-wave parameters, including F-wave conduction velocity, persistence, and amplitude for the tibial nerve in cats. ANIMALS: 30 clinically normal cats. PROCEDURES: F-waves elicited in the interosseous muscles by stimulation of the tibial nerve were recorded, and linear regression analyses of the shortest latency versus the length of the tibial nerve and the limb length were performed. F-wave persistence was calculated by dividing the number of recorded F-waves by the number of stimuli. RESULTS: The correlation coefficient between F-wave latency and nerve length was 0.92, and that between F-wave latency and limb length was 0.58. Mean +/- SD F-wave conduction velocity of the tibial nerve was calculated to be 97.1 +/- 5.0 m/s. Linear regression analysis yielded the regression equation as follows: F-wave latency (milliseconds) = 2.60 + (0.02 x nerve length [mm]). Mean F-wave persistence and amplitude were 98.7 +/- 2.3% and 1.01 +/- 0.62 mV, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that nerve length should be used for nerve conduction studies of F-waves in felids. The regression equation for F-wave latency, conduction velocity, persistence, and amplitude may contribute to the diagnosis of nervous system diseases or injury in cats, such as trauma to the spinal cord or diabetic neuropathy.  相似文献   

Bacteriocin production of probiotic Lactobacillus gasseri LA39 isolated from human feces in milk-based media     

Kensuke ARAKAWA  Yasushi KAWAI  Kenji FUJITANI  Junko NISHIMURA  Haruki KITAZAWA  Ken-ichi KOMINE  Kenzo KAI  Tadao SAITO 《Animal Science Journal》2008,79(5):634-640

The use of bacteriocins from Lactobacillus gasseri , a probiotic lactic acid bacterium, as bio-preservatives in the food industry and animal formulations has been limited because few strains of Lb. gasseri are cultivated and produce a bacteriocin in natural media such as milk and milk-based media. By the determination of the growth-supplements to milk among the 47 nutrients, Lb. gasseri JCM1131^T, LA39 and LA158 isolated from human feces were successfully cultured in reconstituted skim milk and cheese whey using proteose peptone as a nutrient supplement, where Lb. gasseri LA39 produced a useful bacteriocin, gassericin A, with effective growth-inhibiting activity against Gram-positive food-borne pathogens. The data suggest these developed low-cost safe media supporting enough production of bacteriocins by the probiotic Lb. gasseri LA39 could be used to improve the safe bio-preservation of foods and therapy of bovine mastitis, and extra cheese whey produced by cheese making industry is reused in the cultivation for probiotics effectively.  相似文献   

Evaluation of basal media for micropropagation of four highbush blueberry cultivars     

Takuya Tetsumura  Yasuyo Matsumoto  Makiko Sato  Chitose Honsho  Kensuke Yamashita  Haruki Komatsu  Yasuhiro Sugimoto  Hisato Kunitake 《Scientia Horticulturae》2008

Murashige and Skoog medium (MS), woody plant medium (WPM), and a mixture of equal parts of MS and WPM (MW) were compared for in vitro shoot proliferation and rooting of four highbush blueberry (Vaccinium spp.) cultivars. During the multiplication stage, the shoots on WPM showed worse growth than the other shoots. The MW produced the best shoot growth. The shoots on MS grew well but tended toward hyperhydricity in the multiplication stage, especially in the case of ‘Bluecrop’ shoots. Rooting was worst in the shoots multiplied on WPM, while the best rooting percentage of ‘Bluecrop’ shoots was obtained on MS, and that of ‘O’Neal’ on MW. The rooted shoots developed a good root system and were easily acclimatized after potting.  相似文献   

Diagnostic utility of cardiac troponin I in cats with hypertrophic cardiomyopathy          下载免费PDF全文

Yasutomo Hori  Masayuki Iguchi  Yasuhiro Heishima  Yohei Yamashita  Kensuke Nakamura  Atsushi Hirakawa  Akihito Kitade  Toshiki Ibaragi  Michio Katagi  Tamotsu Sawada  Masashi Yuki  Nobuyuki Kanno  Haruki Inaba  Noriko Isayama  Hideyuki Onodera  Naoki Iwasa  Mikio Kino  Mikihiro Narukawa  Syuhei Uchida 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):922-929

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Effects of correcting missing daily feed intake values on the genetic parameters and estimated breeding values for feeding traits in pigs          下载免费PDF全文

Tetsuya Ito  Kazuo Fukawa  Mai Kamikawa  Satoshi Nikaidou  Masaaki Taniguchi  Aisaku Arakawa  Genki Tanaka  Satoshi Mikawa  Tsutomu Furukawa  Kensuke Hirose 《Animal Science Journal》2018,89(1):12-20

Daily feed intake (DFI) is an important consideration for improving feed efficiency, but measurements using electronic feeder systems contain many missing and incorrect values. Therefore, we evaluated three methods for correcting missing DFI data (quadratic, orthogonal polynomial, and locally weighted (Loess) regression equations) and assessed the effects of these missing values on the genetic parameters and the estimated breeding values (EBV) for feeding traits. DFI records were obtained from 1622 Duroc pigs, comprising 902 individuals without missing DFI and 720 individuals with missing DFI. The Loess equation was the most suitable method for correcting the missing DFI values in 5–50% randomly deleted datasets among the three equations. Both variance components and heritability for the average DFI (ADFI) did not change because of the missing DFI proportion and Loess correction. In terms of rank correlation and information criteria, Loess correction improved the accuracy of EBV for ADFI compared to randomly deleted cases. These findings indicate that the Loess equation is useful for correcting missing DFI values for individual pigs and that the correction of missing DFI values could be effective for the estimation of breeding values and genetic improvement using EBV for feeding traits.  相似文献   

Production of a bacteriocin‐like inhibitory substance by Leuconostoc mesenteroides subsp. dextranicum 213M0 isolated from Mongolian fermented mare milk,airag          下载免费PDF全文

Kensuke Arakawa  Saki Yoshida  Hiroki Aikawa  Chihiro Hano  Tsognemekh Bolormaa  Sedkhuu Burenjargal  Taku Miyamoto 《Animal Science Journal》2016,87(3):449-456

Strain 213M0 was selected with productivity of a bacteriocin‐like inhibitory substance (BLIS) among 235 strains of lactic acid bacteria (LAB) isolated from Mongolian fermented milk ‘airag’. Strain 213M0 was species‐identified as Leuconostoc mesenteroides subsp. dextranicum by morphological observation, carbohydrate fermentation profiling and sequencing the 16S rRNA gene. Incubation temperature proper to produce the BLIS was 25°C rather than 30 and 37°C, and the production actively proceeded during the exponential growth phase of the producer cells. Antibacterial effect of BLIS 213M0 was limited to all nine strains of Listeria sp. bacteria and seven strains of LAB cocci among 53 tested strains, which corresponds to a typical feature of the class IIa pediocin‐like bacteriocins. BLIS 213M0 was not inactivated in every broad pH range solution (pH 2.0‐11.0), and was stable against storage at 25°C for 1 week and heating at 121°C for 15 min under pH 4.5. Peptide frame of BLIS 213M0 was confirmed by inactivation with some peptidases, and then its molecular weight was estimated to be 2.6‐3.0 kDa using an in situ activity assay following sodium dodecyl sulfate polyacrylamide gel electrophoresis. The estimated size was different from the other Leuconostoc bacteriocins already reported. These results suggest that BLIS 213M0 would be a novel listericidal bacteriocin.  相似文献   

Density effects on the conifer mortality in declining spruce-fir forest in northern Japan: Implication of bark beetle attack to, cause spruce decline     

Kenichi Ozaki  Kenji Fukuyama  Kaoru Maetô  Kensuke Itoh 《Journal of Forest Research》1998,3(4):231-235

We examined mortality patterns of two conifer species in relation to tree abundance and species composition in a declining spruce-fir forest in Akan National park, Hokkaido, northern Japan. The data taken from eleven 58 m × 58 m square study plots showed that percentage basal area (BA) of dead trees of canopy trees during the last decade was 49% forPicea jezoensis (Sieb. et Zucc.) Carr and 30% forAbies sachalinensis (Fr. Schm.) Masters. InP. jezoensis, percentage basal area (BA) of dead trees was positively related to both BA of its own species and proportion of it to BA of all species, whereas it was not significantly related to both of these variables inA. sachalinensis. Multiple logistic regression with dbh as a confounding factor also indicated that, inP. jezoensis, the proportion of dead stems was positively affected by the stem density of conspecific trees, whereas it was negatively affected by the stem density of the other species in each study plot. However, inA. sachalinensis, the proportion of dead stems was significantly related to neither of these factors. The different mortality pattern between two conifer species suggests that the spruce bark beetle,Ips typographus (L.), which is a serious pest of spruce, may have caused theP. jezoensis decline. Instead of wind-throw or thinning that usually initiate this bark beetle attack, some unknown factors seemed to predispose the trees to insect attack. These unknown factors may also explain the high mortality ofA. sachalinensis in this forest. This research was supported by FFPRI Project, Ministry of Agriculture, Forestry and Fishery.  相似文献   

Effects of three infusion fluids with different sodium chloride contents on steady‐state serum concentrations of bromide in dogs          下载免费PDF全文

Koya Fukunaga  Hikaru Matsumoto  Michiko Wate  Karin Misawa  Miyoko Saito  Hiroshi Matsuda  Kensuke Orito 《Journal of veterinary pharmacology and therapeutics》2018,41(5):684-690

Potassium bromide overdose (bromism) in the management of canine epilepsy has been known. However, a protocol to reduce bromide concentrations rapidly has not been previously established. The effects of three infusion fluids with different chloride contents on the steady‐state serum concentrations of bromide in beagles were determined. After stabilization of the serum bromide concentrations, seven dogs were infused with saline (Na⁺ 154 mmol/L; Cl^? 154 mmol/L), lactated Ringer's (Na⁺ 131 mmol/L; Cl^? 110 mmol/L), or maintenance solutions (Na⁺ 35 mmol/L; Cl^? 35 mmol/L) at a rate of 2 or 10 ml kg^?1 hr^?1 for 5 hr. Serum and urine were collected hourly, and the bromide concentrations were measured. When saline and lactated Ringer's solutions were infused at a rate of 10 ml kg^?1 hr^?1 for 5 hr, serum bromide concentrations were decreased by 14.24% and urine bromide concentrations by 17.63%, respectively. Of all compositions of infusion fluids, only sodium and chloride contents were associated with the decreased serum concentrations and the increased renal clearance of bromide. In summary, saline and lactated Ringer's solutions reduced serum bromide concentrations in a sodium chloride‐dependent manner in dogs were found when infused at 10 ml kg^?1 hr^?1 for 5 hr.  相似文献   

Antibody titers for canine parvovirus type-2, canine distemper virus, and canine adenovirus type-1 in adult household dogs     

Taguchi M  Namikawa K  Maruo T  Orito K  Lynch J  Sahara H 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(9):983-986

Serum antibody titers for canine parvovirus type-2 (CPV-2), canine distemper virus (CDV) and canine adenovirus type-1 (CAV-1) were investigated in 1031 healthy adult household dogs (2 to 18 years old) given an annual inoculation in the previous 11 to 13 months. The number of dogs retaining significant titers of antibodies against CPV-2, CDV, and CAV-1 were 888 (86%), 744 (72%), and 732 (71%), respectively. There were no differences between males and females in antibody titers against the 3 viruses. Antibody titer for CPV-2 was significantly higher in younger dogs than in older dogs, CDV antibody was significantly higher in older dogs than in younger dogs, and CAV titer was not associated with age.  相似文献   

Spontaneous Adenosquamous Carcinoma with Rapid Growth and EMT-like Changes in the Mammary Gland of a Young Adult Female BALB/c Mouse     

Katsumi Takaba  Teruyoshi Imada  Shigehisa Katsumata  Hiroshi Okumura  Sachiko Iwamoto  Yui Suzuki  Minami Imaizumi  Kensuke Myojo  Chie Takada  Naoya Kimoto  Koji Saeki  Itaru Yamaguchi 《Journal of toxicologic pathology》2012,25(4):265-271

This study histopathologically and immunohistochemically investigated a spontaneously occurring single mass subcutaneously located in the left lower abdomen of a female BALB/cAJcl−nu/+ mouse at 10 weeks of age. The mass was about 20 × 15 × 10 mm in size after formalin fixation; nevertheless, it was not detected by clinical observations at 9 weeks of age. H&E staining revealed the tumor origin was epithelial and probably arose from the mammary gland, and the tumor cells demonstrated a squamous, acinar or polyhedral/basal pattern. A cell kinetics analysis revealed that many of the tumor cells of the squamous, acinar or polyhedral/basal component were positive for PCNA and cyclin D1, although there were a few of TUNEL-positive tumor cells in all of the components. An epithelial/mesenchymal analysis demonstrated that most of the tumor cells of the squamous and acinar components contained keratin and E-cadherin; however, most of the tumor cells of the polyhedral/basal component were less or very weakly positive for these markers. The tumor cells of the squamous component were negative for vimentin and SMA; however, many of the tumor cells of the polyhedral/basal component exhibited vimentin. In addition, expression of SMA was confirmed in some tumor cells of the acinar and basal components. Based on the microscopic and immunohistochemical characterizations, the tumor was diagnosed to be adenosquamous carcinoma that originated from the mammary gland with rapid growth, and the tumor cells demonstrated epithelial-mesenchymal transition-like changes.  相似文献