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101.
A conservation experiment is described in which the losses in ensiling heavily-wilted herbage, of approximately 50% dry matter, in lined trench silos, with and without a polyethylene film seal, are compared. The composition and digestibility of the silages produced in the sealed silos were comparable with those of the original herbage. The content of organic acids in the sealed silos was low, suggesting that fermentation was suppressed by the partially gas-tight seal. In the unsealed silos there was considerable spoilage, 70% of the material being inedible. In the 2 sealed silos losses of dry matter were 8·2 and 5·2% and losses of SE 11·5 and 7·8%, respectively. The losses recorded are compared with those usually encountered in lined trench silos. 相似文献
102.
Effect of Oil Overlay on Inhibition Potential of Roscovitine in Sheep Cumulus‐Oocyte Complexes 下载免费PDF全文
LF Crocomo WC Marques Filho CMV Ulian NS Branchini DT Silva CL Ackermann FC Landim‐Alvarenga SD Bicudo 《Reproduction in domestic animals》2015,50(3):410-416
Inhibitors of cyclin‐dependent kinases, as roscovitine, have been used to prevent the spontaneous resumption of meiosis in vitro and to improve the oocyte developmental competence. In this study, the interference of oil overlay on the reversible arrest capacity of roscovitine in sheep oocytes as well as its effects on cumulus expansion was evaluated. For this, cumulus‐oocyte complexes (COCs) were cultured for 20 h in TCM 199 with 10% foetal bovine serum (Control) containing 75 μm roscovitine (Rosco). Subsequently, they were in vitro matured (IVM) for further 18 h in inhibitor‐free medium with LH and FSH. The culture was performed in Petri dishes under mineral oil (+) or in 96 well plates without oil overlay (?) at 38.5°C and 5% CO2. At 20 and 38 h, the cumulus expansion and nuclear maturation were evaluated under stereomicroscope and by Hoechst 33342 staining, respectively. No group presented cumulus expansion at 20 h. After additional culture with gonadotrophins, a significant rate of COCs from both Control groups (+/?) exhibited total expansion while in both Rosco groups (+/?) the partial expansion prevailed. Among the oocytes treated with roscovitine, 65.2% were kept at GV in the absence of oil overlay while 40.6% of them reached MII under oil cover (p < 0.05). This meiotic arrest was reversible, and proper meiosis progression also occurred in the Control groups (+/?). So, the culture system without oil overlay improved the meiotic inhibition promoted by roscovitine without affecting the cumulus expansion rate or the subsequent meiosis progression. 相似文献
103.
Pawson S. M. Kerr J. L. Kimberley M. O. Meurisse N. Somchit C. Wardhaugh C. W. 《Journal of pest science》2021,94(4):1375-1392
Journal of Pest Science - Ecologically and economically harmful wood borers and bark beetles, which have the capacity to expand geographically through the international log trade, require... 相似文献
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Two experiments were conducted to study the effect on nutrient loss and feeding value of making hay by different means, using a barn-drier, a forage-harvester, a crimper and conventional tedding. Both forage-harvesting and crimping of the herbage significantly increased the rate of drying, compared with tedding alone. In Exp. 2 this effect enabled the crimped and forage-harvested hays to be baled for storage 3 days earlier than the tedded hay. The use of the forage-harvester significantly reduced the yield of herbage compared with conventional mowing. Nutrient losses from cutting until storage were highest for the forage-harvested hays, the loss of dry matter between cutting and storage in the forage-harvested hays being 39·9 and 19·3%, respectively, in Expt 1 and 2 with corresponding losses in the conventionally made hays of 7·1% and 9·3%. Crimping the hay resulted in higher nutrient losses than tedding. In Expt 2 there was a loss of dry matter of 17·5% in the conventionally made hay during an 18-week storage period, compared with 3·6% in barn-dried hay, 10·8% in forage-harvested hay and 8·8% in crimped hay.
In Expt 2, in which digestibility determinations were made, conservation decreased the total digestible nutrients and starch equivalent in all treatments. The use of the barn-drier gave the most efficient conservation of total digestible nutrients, followed by the crimped, forage-harvested and conventional treatments in that order. The starch equivalent values of hays made from the same sward were 43·0 when barn-dried, 39·8 when crimped, 38·6 after forage-harvesting and 31·6 after tedding. 相似文献
In Expt 2, in which digestibility determinations were made, conservation decreased the total digestible nutrients and starch equivalent in all treatments. The use of the barn-drier gave the most efficient conservation of total digestible nutrients, followed by the crimped, forage-harvested and conventional treatments in that order. The starch equivalent values of hays made from the same sward were 43·0 when barn-dried, 39·8 when crimped, 38·6 after forage-harvesting and 31·6 after tedding. 相似文献
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