Variations in dark respiration and mitochondrial numbers within needles of Pinus radiata grown in ambient or elevated CO2 partial pressure     

Griffin KL  Anderson OR  Tissue DT  Turnbull MH  Whitehead D 《Tree physiology》2004,24(3):347-353

Within-leaf variations in cell size, mitochondrial numbers and dark respiration rates were compared in the most recently expanded tip, the mid-section and base of needles of Pinus radiata D. Don trees grown for 4 years in open-top chambers at ambient (36 Pa) or elevated (65 Pa) carbon dioxide partial pressure (p(CO2)a). Mitochondrial numbers and respiratory activity varied along the length of the needle, with the highest number of mitochondria per unit cytoplasm and the highest rate of respiration per unit leaf area at the base of the needle. Regardless of the location of the cells (tip, middle or basal sections), needles collected from trees grown in elevated p(CO2)a had nearly twice the number of mitochondria per unit cytoplasm as those grown in ambient p(CO2)a. This stimulation of mitochondrial density by growth at elevated p(CO2)a was greater at the tip of the needle (2.7 times more mitochondria than in needles grown in ambient CO2) than at the base of the needle (1.7 times). The mean size of individual mitochondria was unaffected either by growth at elevated p(CO2)a or by position along the needle. Tree growth at elevated p(CO2)a had a variable effect on respiration per unit leaf area, significantly increasing respiration in the tip of the needles (+25%) and decreasing respiration at the mid-section and base of the needles (-14% and -25%, respectively). Although a simple relationship between respiration per unit leaf area and mitochondrial number per unit cytoplasm was found within each CO2 treatment, the variable effect of growth at elevated p(CO2)a on respiration along the length of the needles indicates that a more complex relationship must determine the association between structure and function in these needles.  相似文献   

An AHP Assessment Of Barriers in Adopting Sustainable Forest Management Practices in The Context Of Carbon Emission Reductions in Cameroon     

Eugene L. Chia  Didier Hubert  Kevin Enongene  Yitagesu T. Tegegne 《Journal of Sustainable Forestry》2020,39(4):379-391

ABSTRACT

As the role of forests in climate change mitigation is explicitly recognized in the Paris Agreement, the need to enhance the adoption of Sustainable Forest Management (SFM) practices is crucial. Therefore, this paper aims at identifying and evaluating barriers in adopting SFM practices in the context of forest carbon emission reductions. A total of 15 barriers in adopting SFM practices are listed through literature and expert inputs. Using Cameroon as a case study, the listed barriers are then evaluated by experts to determine their relative importance using the Analytical Hierarchy Process (AHP) method. According to our findings, the ‘Regulatory and Legislative frameworks’ barrier category was attributed to the highest importance among other categories, in adopting SFM practices in the forestry sector. ‘Inadequate political will and incentive to enforce regulations’ appears to be the major obstacle in adopting SFM practices in Cameroon. As carbon emission reduction initiatives are being developed, there is need to move from broad to concrete suggestions that will overcome these barriers. However, proper diagnosis is necessary in order to target barriers with the right incentives and enabling conditions that will support carbon programs and projects to deliver effective emission reductions.  相似文献   

Early height development and species stratification across five climax series in the eastern Washington Cascade range     

Kevin L. O'Hara 《New Forests》1995,9(1):53-60

Reconstruction in twenty single-cohort stands across five climax series on the eastern slope of the Washington Cascade Range found a variety of species combinations and development patterns. Western larch (Larix occidentalis Nutt.) and lodgepole pine (Pinus contorta Dougl. ex Loud) were found to be very competitive species that usually occupied a dominant position in stands in the Abies grandis, Abies lasiocarpa, and Tsuga mertensiana climax series. Interior Douglas-fir (Pseudotsuga menziesii var. glauca (Mirb.) Franco) was found in all five climax series and, although its height growth was less than that of western larch or the lodgepole pine, it was usually found in the upper stratum. These results suggest that site classification based on climax potential should be used cautiously when applied to young stand management decisions regarding seral species. For example, the difference between an Abies grandis, and a Tsuga mertensiana climax series is quite large in terms of potential productivity but in either case western larch, if present, will likely dominate these stands.  相似文献   

Stomatal and non-stomatal limitations to photosynthesis in four tree species in a temperate rainforest dominated by Dacrydium cupressinum in New Zealand     

Tissue DT  Griffin KL  Turnbull MH  Whitehead D 《Tree physiology》2005,25(4):447-456

We assessed the relative limitations to photosynthesis imposed by stomatal and non-stomatal processes in Dacrydium cupressinum Lamb. (Podocarpaceae), which is the dominant species in a native, mixed conifer-broad-leaved rainforest in New Zealand. For comparison, we included three co-occurring broad-leaved tree species (Meterosideros umbellata Cav. (Myrtaceae), Weinmannia racemosa L.f. (Cunoniaceae) and Quintinia acutifolia Kirk (Escalloniaceae)) that differ in phylogeny and in leaf morphology from D. cupressinum. We found that low foliage phosphorus content on an area basis (P(a)) limited light-saturated photosynthesis on an area basis (A(sat)) in Q. acutifolia. Depth in the canopy did not generally affect A(sat) or the relative limitations to A(sat) because of stomatal and non-stomatal constraints, despite reductions in the ratio of foliage mass to area, foliar nitrogen on an area basis (N(a)) and P(a) with depth in the canopy. In the canopy-dominant conifer D. cupressinum, A(sat) was low, consistent with low values of the maximum rate of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) carboxylation (V(cmax)). In comparison, the A(sat) response of the three broad-leaved tree species was quite variable. Although A(sat) was high in the canopy-dominant M. umbellata, it was low in the sub-canopy trees W. racemosa and Q. acutifolia. Relative stomatal limitation to photosynthesis was more pronounced in W. racemosa (40%) than in the other three species (28-33%). Despite differences in degree, non-stomatal limitation to A(sat) predominated in all tree species.  相似文献   

The historical development of uneven-aged silviculture in North America   总被引：2，自引：0，他引：2  

O'Hara  Kevin L. 《Forestry》2002,75(4):339-346

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Assessment of allometric algorithms for estimating leaf biomass, leaf area index and litter fall in different-aged Sitka spruce forests   总被引：1，自引：0，他引：1  

Tobin  Brian; Black  Kevin; Osborne  Bruce; Reidy  Brian; Bolger  Tom; Nieuwenhuis  Maarten 《Forestry》2006,79(4):453-465

The relationship between leaf area and diameter at breast height(d.b.h.) or sapwood area (AS) has been used to estimate standleaf area or biomass of forest canopies. It has been suggestedthat intra-specific variations in the relationship between standleaf area and d.b.h. or AS can introduce a systematic errorin these estimates for younger and older stands unless additionalparameters relating to canopy structure are included in allometricfunctions. We collected data from a Sitka spruce chronosequenceto parametrize and test different algorithms for the estimationof foliar biomass (FB) and litter inputs over a range of forestages. FB estimates were significantly improved when additionalbiometric information relating to crown structure (canopy opennessand height of live crown) was included in the models. Althoughthe use of the relationship between leaf area and AS for theestimation of leaf area is justified by theoretical considerations(pipe model theory), we show that d.b.h. and other canopy parametersprovided the most robust estimation of leaf area across different-agedstands. Our results also suggest that the accuracy of litterinput estimates depends on needle retention time and annualturnover rate, particularly immediately before and after canopyclosure.  相似文献   

Overstory and shrub effects on natural regeneration processes in native Pinus radiata stands     

Michael J. O&#x;Brien  Kevin L. O&#x;Hara  Nadir Erbilgin  David L. Wood 《Forest Ecology and Management》2007,240(1-3):178-185

Native Monterey pine (Pinus radiata D. Don) stands on the Monterey peninsula have been significantly modified by natural and anthropogenic disturbances. The exclusion of fire and the introduction of pitch canker (caused by Fusarium circinatum Nirenberg & O’Donnell) created a need for the examination of these changing ecosystems with respect to regeneration, especially as these stands reach mature ages. We established 210 plots on 35 transects distributed throughout five stands in order to describe the current stand structure and quantify the extent and condition of regeneration. The results indicated that Monterey pine seedling establishment varies throughout the peninsula depending on percent canopy cover, duff and litter depth, and percent shrub cover while seedling growth is influenced by percent shrub cover. Canopy cover was also found to inversely influence shrub cover. Our study highlights the importance of understory removal to increase Monterey pine regeneration and seedling growth as canopy cover decreases, especially in areas where coast live oak (Quercus agrifolia Nee) and poison-oak (Toxicodendron diversilobum E. Greene) are abundant.  相似文献   

不同提取方法山苍子油的化学成分与抗氧化活性分析     

李芳  Yaru Song  Howard Holness  Kevin O’Shea 《林业科学》2015,(3):124-131

【目的】比较不同提取方法对山苍子油提取效率与抗氧化活性的影响,通过 GC-MS分析,研究山苍子油成分构成与抗氧化活性的内在关联,分析山苍子油抗氧化活性的主要物质基础。【方法】采用液液萃取(分别加以超声波与磁化辅助处理)、二氧化碳超临界萃取以及水蒸气蒸馏法提取山苍子油,DPPH 法比较不同提取方式对山苍子油抗氧化活力的影响,GC-MS分析山苍子油的主要成分,并以1,4－二溴苯为内标,采用面积归化法解析主要成分的相对含量。【结果】山苍子油具有显著的抗氧化活性。液液萃取法的提取效率与山苍子油抗氧化活性最高,超声波辅助处理油得率最高,为26.3%,磁化辅助处理抗氧化活性最优,达到31.22 mg·mL －1( IC50),二氧化碳超临界萃取法次之,抗氧化活性为56.95 mg·mL －1( IC50),水蒸气蒸馏法抗氧化活性最弱,为64.95 mg·mL －1( IC50); GC-MS分析检测出122种以上的化合物,包括12种脂肪酸,16种萜烯,18种含氧萜烯以及其他微量化合物包括烯烃、醇、酮类与烷烃等。GC-MS分析结果表明：山苍子干果主要成分为饱和脂肪酸(月桂酸等)、不饱和脂肪酸(9,12－十八碳二烯酸、9－十八碳烯酸等)以及α蒎烯、β蒎烯、柠檬烯等萜烯、氧化萜烯类物质。液液萃取法得到的山苍子油脂肪酸含量(53.51%～66.61%)明显高于二氧化碳超临界萃取(17.64%)与水蒸气蒸馏法(8.1%),而二氧化碳超临界萃取与水蒸气蒸馏法得到的萜烯、氧化萜烯类含量分别达到29.37%和17.69%,明显高于液液萃取法(6.49%～9.75%)。【结论】磁化辅助处理有利于抗氧化活性物质的提取,且不饱和脂肪酸的含量最高;超声波辅助处理可以提升油的得率;水蒸气蒸馏法和超临界流体萃取法更有利于萜烯类化合物的获取;联系到水蒸气蒸馏法得到的山苍子油富含萜烯化合物却显示较低的抗氧化活性(64.95 mg·mL －1 ),色谱级的柠檬醛抗氧化活性最低(74.33 mg·mL －1),因此推测脂肪酸成分(特别是不饱和脂肪酸)是山苍子油抗氧化活性的主要物质基础。  相似文献   

The Ontario Veterinary College after World War II: 1945–1953     

Kevin Woodger  Elizabeth A. Stone 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(4):399-404

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Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time     

Ben C Maddison  John Spiropoulos  Christopher M Vickery  Richard Lockey  Jonathan P Owen  Keith Bishop  Claire A Baker  Kevin C Gough 《Veterinary research》2015,46(1)

Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation on soil, two PrP^Sc phenotypes were present: G₃₃₈ or Apl₃₃₈ii. Thirteen months later some divergent PrP^Sc phenotypes were seen: a mixture of Apl₃₃₈ii with either G₃₃₈ or P_338, and a completely novel PrP^Sc deposition, designated Cag₃₃₈. The data show that prolonged ageing of scrapie prions within an environmental matrix may result in changes in the dominant PrP^Sc biological/biochemical properties.  相似文献