Analysis of the structure of microbial community in soils with different degrees of salinization using T-RFLP and real-time PCR techniques     

E. E. Andronov  S. N. Petrova  A. G. Pinaev  E. V. Pershina  S. Zh. Rakhimgalieva  K. M. Akhmedenov  A. V. Gorobets  N. Kh. Sergaliev 《Eurasian Soil Science》2012,45(2):147-156

Molecular methods were used to study variation in the taxonomic structure of bacterial, archaeal, and fungal communities in soil samples taken along a salinity gradient from a solonchak in the vicinity of Lake Akkol’ (Shingirlau, Kazakhstan). Soils from arable fields located 195 km from the solonchak served as the control. Total DNA was isolated from every sample and analyzed by T-RFLP and real-time PCR. Salinization was found to be the main ecological factor determining the structure of soil microbial community in the study region. The values of Simpson’s index characterizing the diversity of this community proved to be similar in all the samples, which, however, significantly differed in the taxonomic composition of microorganisms. A significantly increased content of archaea was revealed in the sample with the highest salinity. The results of this study show that the structure of soil microbial community reflects specific features of a given soil and can be used as an indicator of its ecological state.  相似文献   

Microsatellite mapping of the genes for sham ramification and extra glume in spikelets of tetraploid wheat     

Y. Amagai  A. J. Aliyeva  N. Kh. Aminov  P. Martinek  N. Watanabe  T. Kuboyama 《Genetic Resources and Crop Evolution》2014,61(2):491-498

The loci for expression of prolonged spikelet rachilla named ‘sham ramification’ in three tetraploid wheat accessions, two accessions of Triticum jakubzineri Udacz. et Schachm. (2n = 4× = 28, genome BBAA) ‘PI 585014’ and ‘R-101-03’ and one sample of Triticum turgidum L. (2n = 4× = 28, genome BBAA) ‘PI 67339’, were mapped by genotyping F₂ populations using microsatellite markers. The segregation analysis confirmed that sham ramification was controlled by recessive, epistatic genes, shr1 (sham ramification 1) and shr2 (sham ramification 2). The genotypes of ‘sham ramification’ were shr1shr1Shr2Shr2 for T. jakubzineri and Shr1Shr1shr2shr2 for T. turgidum (PI 67339). Normal rachilla was determined by Shr1Shr1Shr2Shr2. The shr1 gene and the gene for extra glume (exg) were completely linked; and, the shr1/exg gene complex was bracketed by the markers Xbarc319 and Xbarc232 on the long arm of chromosome 5A. In the F₂ of PI 67339/LD222 the gene shr2 was bracketed by Xwmc819 and Xwmc794 on the long arm of chromosome 2A.  相似文献   

Influence of extruded carbamide concentrates on nitrogen exchange and meat efficiency of bull calves     

V. I. Levakhin  B. Kh. Galiev  R. F. Mangutov  I. A. Rakhimzhanova  R. Sh. Abdulgazizov 《Russian Agricultural Sciences》2014,40(4):278-281

Extrusion of granular white with carbamide (20%) allows us to get a concentrate with crude protein content of 64.8% in dry substance. It is reasonable to balance mixed fodders by protein carbamide concentrates. For conducting the experiment on bull calves of Simmental breed, three compound feed formulae have been developed. Mixed fodders of two formulae contain carbamide concentrate. In one of the mixed fodder formulae, 10% of wheat coat is replaced with elementary sulphur and beet molasses was added to the other one. The usage of these concentrates increased nitrogen deposits in organisms, meat productivity, meat quality and profitability level of beef production.  相似文献   

Means of prevention of trichodesmotoxicosis     

N Kh Shevchenko  F I Ibadullaev 《Veterinariia》1974,(10):113-115

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Effects of thiamine preparations of biocomplexes of the blood in calves     

A F Rush  R Kh Karmoliev 《Veterinariia》1974,(10):115-116

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Piroplasmosis and measures for its control     

I S Umarov  I Kh Rasulov  S N Khuda?nazarova  B S Ovezmuradov  B M Divanov 《Veterinariia》1974,(9):64-67

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Effective preparations in colibacillosis of calves and piglets]     

V M Petrov  P K Anan'ev  V A Gushul  E B Solov'ev  Kh R Shol' 《Veterinariia》1978,(5):53-56

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Assessing the efficacity of Cyazid in dictyocauliasis in sheep     

A A Vasil'ev  Iu Ia Dol'nikov  M A Vorob'ev  M Kh Gafarov  I A Buldakovski? 《Veterinariia》1969,46(6):55-56

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Ecological soil science     

A. Kh. Sheudzhen  G. A. Galkin 《Eurasian Soil Science》2006,39(12):1370-1370

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Physicochemical principles of the fractal organization of soil colloids     

G. N. Fedotov  G. V. Dobrovol’skii  V. I. Putlyaev  E. I. Pakhomov  A. I. Kuklin  A. Kh. Islamov 《Eurasian Soil Science》2007,40(7):740-746

An electron-microscopic study was conducted of gel films collected from aggregates from humus-accumulative horizons of chernozem and soddy-podzolic soils. The aggregates were dried and then capillary-wetted and immersed in water, Solutions obtained by pressing from these soils were also studied. Based on the results obtained, a hypothetical mechanism was proposed for the development of fractal organization of soil colloids, which involves the fixation of micron-size mineral particles in the humus gel and their transformation under the effect of aggressive substances with the formation of colloidal particles of reaction products diffusing in the humus gel. Humus macromolecules contain many polar groups; therefore, the colloidal particles pass some distance and are then fixed on these groups. The greater the distance from a coarse particle in the center of a cluster the smaller the number of colloidal particles capable of traversing it. Therefore, the concentration of colloidal particles decreases when going from the cluster center to its periphery according to an exponential law, which results in the development of the fractal organization in the colloidal soil component. Results of soil studies using the small-angle neutron scattering method were analyzed in terms of the hypothesis proposed.  相似文献