Improvement of growth and nitrogen utilization in sheep using sugar beet pulp treated with Trichoderma reesei or urea     

Okab AB  Ayoub MA  Samara EM  Abdoun KA  Al-Haidary AA  Koriem AA  Hassan AA 《Tropical animal health and production》2012,44(7):1623-1629

Twenty-five intact Barki lambs with mean body weight of 24.81 ± 0.16?kg were used to investigate the effect of including in the diet sugar beet pulp (SBP) treated biologically with Trichoderma reesei or chemically with urea 4 % on nutrients digestibility, growth performance, nitrogen (N) utilization, and hematological and biochemical parameters. Two experiments were conducted. In the growth experiment, five lambs were randomly assigned to one of five dietary treatments. Lambs were offered isonitrogenous and isoenergetic concentrate feed mixture containing on dry matter basis 0 % SBP (D0), 50 % SBP (D1), 50 % SBP treated with 4 % urea (D2), 50 % SBP treated with T. reesei (D3), and 25 % SPB treated with 4 % urea plus 25 % SPB treated with T. reesei (D4). In the metabolism experiment, five rams were used in a 5?×?5 Latin square design and housed in metabolism crates for 21?days. The present study showed that inclusion of SBP at the level of 50 % (D1) negatively affected diet digestibility coefficients of crude protein, crude fiber, and ether extract, in addition to average daily gain, feed conversion, and N utilization. However, treatment of SBP with urea (D2), T. reesei (D3), or the combination (D4) of both had improved (P?相似文献   

Geometry of the femoral condyles in dogs     

Ocal MK  Sevil-Kilimci F  Yildirim IG 《Veterinary research communications》2012,36(1):1-6

The stifle joint is one of the most important joints in dogs from the orthopaedic point of view. The aim of this study was to document the morphometric values of femoral condyles, given the close relationship between the shape and function of an anatomic structure. The left femora of 16 mid-sized dogs were used, and diameter and nine radii as well as cranial and caudal bow lengths from each condyle were measured. The photographs were taken of the distal femora from both sides. All measurements were obtained from these images by using software. Additionally, the rotation angle was calculated from the intercondylar distance and the difference between lateral and medial bow lengths. In addition to the rotation angle, the difference of diameter and nine radii between the lateral and medial condyles was determined. All radii except getting at 90° were significantly different between the medial and lateral condyles. The greatest values were determined in the caudal part of the medial condyle. This results the smaller contact area and a greater pressure on the underlying surface, and therefore the meniscus and articular cartilage of the caudal part of the medial side suggests the possibility of a risk of injury as the stifle joint flexes. The mean rotational angle of the femur was also calculated to be 2.18° laterally and 1.02° medially in the caudal and cranial parts of condyles, respectively. The result of this study showed that the lateral and medial condyles had different shapes in canine femur.  相似文献   

Soya-lecithin in extender improves the freezability and fertility of buffalo (Bubalus bubalis) bull spermatozoa     

Akhter S  Ansari MS  Andrabi SM  Rakha BA  Ullah N  Khalid M 《Reproduction in domestic animals》2012,47(5):815-819

Egg yolk is routinely used as a cryoprotectant in semen extenders. However, it may contain cryoprotective antagonists, and there are hygienic risks associated with its use. Proteins of plant origin, like soya-lecithin, lack these hazards. The aim of this study was to use soya-lecithin as a cryoprotectant in extender and to investigate its effects on in vitro quality and in vivo fertility of buffalo semen. Semen from three buffalo bulls was frozen in tris-citric extender containing 5.0%, 10% or 15% soya-lecithin or 20% egg yolk. Sperm motility, plasma membrane integrity and viability were assessed post-dilution, pre-freezing and post-thaw. In Post-dilution and pre-freezing, the values for motility, plasma membrane integrity and viability remained higher (p ≤ 0.05) in extenders containing 10% soya-lecithin and control compared with extender containing 5% and 15% soya-lecithin. However, motility, plasma membrane integrity and viability were higher (p < 0.05) in extender containing 10% soya-lecithin compared with control and extenders containing 5% and 15% soya-lecithin. Semen from two buffalo bulls was frozen in tris-citric extender containing either 10% soya-lecithin or 20% egg yolk. Higher (p < 0.05) fertility rate was recorded in buffaloes inseminated with semen containing 10% soya-lecithin (56%) compared with 20% egg yolk (41.5%). The results suggest that 10% soya-lecithin in extender improves the freezability and fertility of buffalo bull spermatozoa and can be used as an alternate to egg yolk in cryopreservation of buffalo semen.  相似文献   

Regional and circadian variations of sweating rate and body surface temperature in camels (Camelus dromedarius)     

Khalid A. ABDOUN  Emad M. SAMARA  Aly B. OKAB  Ahmed A. AL‐HAIDARY 《Animal Science Journal》2012,83(7):556-561

It was the aim of this study to investigate the regional variations in surface temperature and sweating rate and to visualize body thermal windows responsible for the dissipation of excess body heat in dromedary camels. This study was conducted on five dromedary camels with mean body weight of 450 ± 20.5 kg and 2 years of age. Sweating rate, skin and body surface temperature showed significant (P < 0.001) circadian variation together with the variation in ambient temperature. However, daily mean values of sweating rate, skin and body surface temperature measured on seven regions of the camel body did not significantly differ. The variation in body surface temperature compared to the variation in skin temperature was higher in the hump compared to the axillary and flank regions, indicating the significance of camel's fur in protecting the skin from daily variation in ambient temperature. Infrared thermography revealed that flank and axillary regions had lower thermal gradients at higher ambient temperature (T_a) and higher thermal gradients at lower T_a, which might indicate the working of flank and axillary regions as thermal windows dissipating heat during the night. Sweating rate showed moderate correlation to skin and body surface temperatures, which might indicate their working as potential thermal drivers of sweating in camels.  相似文献   

Development of ergosterol, microbial biomass C, N, and P after steaming as a result of sucrose addition, and Sinapis alba cultivation     

Khalid Saifullah Khan  Tanja Müller  Jens Dyckmans  Rainer Georg Joergensen 《Biology and Fertility of Soils》2010,46(4):323-331

A pot experiment was carried out to monitor the recovery of a steaming-reduced microbial biomass (C, N, and P) and fungal ergosterol by sucrose addition. The second objective was to investigate the recovery of a steaming-reduced microbial biomass by white mustard (Sinapis alba) cultivation and its interactions with microbial residues, freshly formed from sucrose addition. Thirty days after steaming, the soil microbial biomass C and N was still significantly reduced by 80%, leading to a rather constant microbial biomass C/N ratio around 7 throughout the experiment. The steaming-induced decreases of microbial biomass P and ergosterol were only roughly 50%, leading to a decrease in the microbial biomass C/P ratio and an increase in the ergosterol-to-microbial biomass C ratio. Sucrose addition led to a 25% reduction in the ergosterol-to-microbial biomass C ratio. Mustard cultivation had significant positive effects on microbial biomass C, N, P, and ergosterol, but the effects were smaller than those of sucrose addition. Cultivating mustard had no significant effects on the C loss or on the incorporation of sucrose C into the microbial biomass. In contrast, the application of sucrose led to a significant decrease in the mustard shoot biomass and especially in the mustard root biomass.  相似文献   

Effect of Phosphorus and Irrigation Levels on Yield,Water Productivity,Phosphorus Use Efficiency and Income of Lowland Rice in Northwest Pakistan   总被引：1，自引：0，他引：1  

Khalid USMAN 《水稻科学》2013,20(1):61-72

With decreasing availability of water for agriculture and increasing demand for rice production, an optimum use of irrigation water and phosphorus may guarantee sustainable rice production. Field experiments were conducted in 2003 and 2004 to investigate the effect of phosphorus and irrigation levels on yield, water productivity (WP), phosphorus use efficiency (PUE) and income of low land rice. The experiment was laid out in randomized complete block design with split plot arrangements replicated four times. Main plot consisted of five phosphorus levels, viz. 0 (P0), 50 (P50), 100 (P100), 150 (P150), and 200 (P200) kg/hm2, while subplots contained of irrigation times, i.e. 8 (I8), 10 (I10), 12 (I12), and 14 (I14) irrigation levels, each with a water depth of 7.5 cm. Mean values revealed that P150 in combination with I10 produced the highest paddy yield (9.8 t/hm2) and net benefit (1 231.8 US$/hm2) among all the treatments. Phosphorus enhanced WP when applied in appropriate combination with irrigation level. The highest mean WP [13.3 kg/(hm2?mm)] could be achieved at P150 with I8 and decreased with increase in irrigation level, while the highest mean PUE (20.1 kg/kg) could be achieved at P100 with I10 and diminished with higher P levels. The overall results indicate that P150 along with I10 was the best combination for sustainable rice cultivation in silty clay soil.  相似文献   

The effect of photoperiod on selected parameters of boar semen     

Damian Knecht  Sebastian Środoń  Karolina Szulc  Kamil Duziński 《Livestock Science》2013,157(1):364-371

The objective of the present research was to determine the effect of increasing and decreasing natural photoperiods on selected parameters of boar ejaculates. The study material consisted of 17 boars: six Polish Large White (PLW) breed, five Polish Landrace (PL) breed, and six Duroc×Pietrain (D×P) crossbreed, all aged between 8 and 12 months at the beginning of the research. Analyses were conducted on 612 ejaculates, which were collected in two experimental periods: an increasing photoperiod (IP) (January–June) and a decreasing photoperiod (DP) (July–December). A statistically proven impact of photoperiod on the volume of semen was observed in all the studied breeds (P=0.004). During the decreasing photoperiod the mean volume of semen was 261.16±75.20 ml and this was almost 17 ml higher than that for the increasing period. For boars involved in the experiment, day length also had a significant impact on the total number of motile spermatozoa (P=0.037). In the increasing photoperiod the mean number was 3.26×10⁹ lower. A decreasing photoperiod has a positive affect on both boars and the parameters of the collected ejaculates, which were observed in the higher number of insemination doses per ejaculate. Between the different breeds, the reactions of boars to photoperiod differed and the most significant influence of photoperiod on semen parameters was noted among D×P breed boars. Least susceptible to changes in day length were PLW breed boars.  相似文献   

Development and Characterization of Calcium-Alginate Beads of Apigenin: In Vitro Antitumor,Antibacterial, and Antioxidant Activities     

Mohammed F. Aldawsari  Mohammed Muqtader Ahmed  Farhat Fatima  Md. Khalid Anwer  Prakash Katakam  Abdullah Khan 《Marine drugs》2021,19(8)

The objective of this work was to develop sustained-release Ca-alginate beads of apigenin using sodium alginate, a natural polysaccharide. Six batches were prepared by applying the ionotropic gelation technique, wherein calcium chloride was used as a crosslinking agent. The beads were evaluated for particle size, drug loading, percentage yield, and in vitro drug release. Particle size was found to decrease, and drug entrapment efficiency was enhanced with an increase in the polymer concentration. The dissolution study showed sustained drug release from the apigenin-loaded alginate beads with an increase in the polymer proportion. Based on the dissolution profiles, BD6 formulation was optimized and characterized for FTIR, DSC, XRD, and SEM, results of which indicated successful development of apigenin-loaded Ca alginate beads. MTT assay demonstrated a potential anticancer effect against the breast cancer MCF-7 cell lines. The antimicrobial activity exhibited effective inhibition in the bacterial and fungal growth rate. The DPPH measurement revealed that the formulation had substantial antioxidant activity, with EC50 value slightly lowered compared to pure apigenin. A stability study demonstrated that the BD6 was stable with similar (f2) drug release profiles in harsh condition. In conclusion, alginate-based beads could be used for sustaining the drug release of poorly water-soluble apigenin while also improving in vitro antitumor, antimicrobial, and antioxidant activity.  相似文献   

Effects of mycorrhizal fungi on root-hair growth and hormone levels of taproot and lateral roots in trifoliate orange under drought stress     

Fei Zhang  Peng Wang  Ying-Ning Zou  Kamil Kuča 《Archives of Agronomy and Soil Science》2019,65(9):1316-1330

A pot experiment was used to evaluate the effects of an arbuscular mycorrhizal fungus (AMF) Funneliformis mosseae on plant growth performance, root-hair growth, and root hormone levels in trifoliate orange (Poncirus trifoliata) seedlings under well-watered (WW) and drought stress (DS). A 9-week mild DS treatment significantly reduced mycorrhizal colonization of 2nd- and 3rd-order lateral roots. Root mycorrhizal colonization was relatively higher in the 2nd- and 3rd-order lateral roots than in the taproot and the 1st-order lateral root under WW and DS. AMF seedlings exhibited significantly higher root-hair density, length (except for the taproot) and diameter in taproot and 1st-, 2nd-, and 3rd-order lateral roots under WW, and considerably higher root-hair density (except for 1st-order lateral root), length (except for 2nd-order lateral root) and diameter under DS. Mycorrhizal inoculation remarkably increased root abscisic acid (ABA), indole-3-acetic acid (IAA), methyl jasmonate, and brassinosteroids (BRs) concentrations under DS, in company with the decrease in root zeatin riboside and gibberellins levels and root IAA effluxes. Root-hair traits were significantly positively correlated with root colonization and root ABA and BRs levels. It is concluded that mycorrhizal plants possessed better root-hair growth to adapt mild DS, which is associated with mycorrhizal colonization and endogenous hormone changes.  相似文献   

The first report on serotyping of bluetongue virus in small ruminants of Khyber Pakhtunkhwa province,Pakistan     

Malik  Amir Iftikhar  Ijaz  Muhammad  Yaqub  Tahir  Shabir  Muhammad Zubair  Avais  Muhammad  Ghaffar  Awais  Ali  Ahmad  Farooqi  Shahid Hussain  Mehmood  Khalid 《Tropical animal health and production》2019,51(4):977-982

Bluetongue virus (BTV), a member of Orbivirus genus (family Reoviridae), is a non-contagious infection of domestic and wild ruminants. The current study was designed to detect various serotypes of BTV in small ruminants of Khyber Pakhtunkhwa (KPK) province of Pakistan, along with their effects on hemato-biochemical parameters. A total of 408 serum samples in four districts (Mansehra, Abbottabad, Swabi, and Kohat) of KPK from small ruminants were screened based on competitive ELISA (cELISA). A total of 204 (50%) samples were found positive for BTV group–specific antibodies. The seropositive samples were processed for the detection of BTV serotypes through real-time polymerase chain reaction (qPCR). Out of 204 cELISA-positive samples, 60 (29.41%) were found positive through qPCR. Three serotypes [6, 8, 9] were detected from Mansehra District and two from Kohat [2, 8] and Abbottabad [6, 8], while only one from Swabi [8]. The serotype “8” was found consistently in all the four study districts. A significant (p?<?0.05) increase in the level of blood urea nitrogen (BUN) and alkaline phosphatase (ALP) was recorded in goats, whereas aspartate aminotransferase (AST) in sheep infected with BTV, compared to healthy animals. The hematological parameters showed significantly (p?<?0.05) raised total leucocyte count (TLC) in both sheep and goats, whereas only hematocrit (HCT) value was increased significantly (p?<?0.05) in infected sheep. This is the first report on serotyping of BTV among small ruminants in Pakistan.

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