Laparoscopy vs. laparotomy for embryo transfer to produce transgenic goats (Capra hircus)     

Sang Tae Shin  Sung Keun Jang  Hong Suk Yang  Ok Keun Lee  Yhong Hee Shim  Won Il Choi  Doo Soo Lee  Gwan Sun Lee  Jong Ki Cho  Young Won Lee 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(1):103-107

This study was performed to produce transgenic Korean native goat (Capra hircus) by laparoscopic embryo transfer (ET) to overcome the limitations of ET performed by laparotomy. Transgenic embryos were produced by DNA pronuclear microinjection of in vivo zygotes. The recipient goats were synchronized for estrus by using an introvaginal progesterone devices as a controlled internal drug-releasing insert (CIDR) for 13 days and injection of 400 IU PMSG 48 h before removal of the insert. Embryos were transferred on day 3 and 4 after removal of the insert. Recipient goats were deprived of feed for 48 h, then suspended in a laparotomy cradle at an angle of 45°. After obtaining a sufficient pneumoperitoneum, the laparoscope and forceps were inserted abdominally through 5 mm trocar sleeves. Examination of the ovaries and uterus was performed and then 213 embryos were transferred into the oviducts via the infundibula of 76 recipient goats. To compare pregnancy rates, ET was also performed by laparotomy in 82 recipient goats. The pregnancies in the recipient goats were diagnosed by ultrasound on day 30 after embryo transfer. The pregnancy rate with laparoscopic ET was significantly higher than with ET performed by laparotomy (46.1% vs. 28.6%, p < 0.05). In addition, the pregnancy rates were compared between ovulated and non-ovulated ovaries of the recipient goats in the laparoscopic ET group. No significant difference was observed between the pregnancy rates of ovulated and non-ovulated ovaries (41.3% vs. 33.3%, p < 0.05) suggesting that ET may also be possible in non-ovulated recipients through artificial rupture of Graafian follicles. These results suggest that laparoscopic ET is a highly efficient method for the transfer of goat embryos.  相似文献   

Extracellular cyclic adenosine monophosphate‐dependent protein kinase A autoantibody and C‐reactive protein as serum biomarkers for diagnosis of cancer in dogs     

Min‐Ok Ryu  Byung‐Gak Kim  Ul‐Soo Choi  Kwan‐Hyuck Baek  Young‐Ki Song  Qiang Li  Kyoung‐Won Seo  Sandra Ryeom  Hwa‐Young Youn  Dong‐Ha Bhang 《Veterinary and comparative oncology》2019,17(1):99-106

Protein kinase A, a cyclic adenosine monophosphate (AMP)‐dependent enzyme, normally exists within mammalian cells; however, in cancer cells, it can leak out and be found in the serum. Extracellular cyclic AMP‐dependent protein kinase A (ECPKA) has been determined to increase in the serum of cancer‐bearing dogs. However, there have been no reports in the veterinary literature on serum ECPKA autoantibody (ECPKA‐Ab) expression in dogs with cancer. The aim of this study was to evaluate ECPKA‐Ab and C‐reactive protein (CRP) as serum biomarkers for cancer in dogs. ECPKA‐Ab and CRP levels were detected by an enzyme‐linked immunosorbent assay in serum samples from dogs with malignant tumours (n = 167), benign tumours (n = 42), or non‐tumour disease (n = 155) and from healthy control dogs (n = 123). ECPKA‐Ab and CRP levels were significantly higher in the dogs with malignant tumours than in those with benign tumours or non‐tumour diseases, as well as in the healthy controls (P < 0.001, Kruskal‐Wallis test). There was a significant positive correlation between the neoplastic index, which was developed using ECPKA‐Ab and CRP levels, and the presence of cancer in dogs (P < 0.001); the area under the receiver‐operating characteristic curve was estimated to be >0.85 (P < 0.001). In conclusion, ECPKA‐Ab is a potential serum biomarker for a broad spectrum of cancers. Combined measurement of CRP and ECPKA‐Ab levels in serum improves the sensitivity and accuracy of a diagnosis of cancer in dogs.  相似文献   

Effects of grass forage species and long‐term period of low quality forage diet feeding on growth performance,nutrient utilization and microbial nitrogen yield in growing wether lambs          下载免费PDF全文

Da‐Hye Kim  Ki‐Choon Choi  Sang‐Houn Song  Toshiyoshi Ichinohe 《Animal Science Journal》2016,87(2):202-208

Six growing lambs were used to evaluate the feeding value of two forage‐based diets in a long‐term feeding period by measuring body weight (BW) gain, digestibility, nitrogen (N) retention and microbial N (MBN) yield. The animals were fed imported low‐quality timothy hay (TH) with concentrate diet (THD) or imported low‐quality Italian ryegrass straw (IR) with concentrate diet (IRD) for 9 months. The forages were offered at 2% BW, and concentrate was fed at 40% of forage intake. The BW gain averaged 82.6 and 66.2 g/day for THD and IRD, respectively, without showing significant difference. Average forage intake (% BW) was significantly greater for IR than for TH, although it was not affected by feeding periods. The digestibility did not differ between diets or periods. The numerically greater (P = 0.06) ratio of retained N to absorbed N for IRD than that for THD was prominent. Neither diet nor period had significant effect on MBN supply and efficiency of MBN synthesis. The results suggest that the IR‐based diet can be also used for long‐term periods of feeding to growing ruminant animals as a grass hay‐based diet without any detrimental effects on nutrient utilization and growth performance.  相似文献   

ISOLATION OF BOVINE LEUCOSIS VIRUS FROM CATTLE     

Y.S. CHUNG 《Australian veterinary journal》1980,56(1):42-43

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Chemical and structural characteristics of conifer needles exposed to ambient air pollution     

Yoon Soo Kim  Jae Ki Lee 《Forest Pathology》1990,20(4):193-200

Conifer needles exposed to ambient air pollutants were observed by a scanning electron microscope (SEM). Predominant changes on needles were the breakdown and the aggregation of wax structures on stomatal chambers. However, the mode of destruction in stomatal wax structures was dependent on the pollutants. Ginkgo biloba seemed to be resistant to air pollutants despite higher amounts of sulfur in the foliage.  相似文献   

Biological evaluation of neopeptins isolated from a Streptomyces strain     

Kim YS  Kim HM  Chang C  Hwang IC  Oh H  Ahn JS  Kim KD  Hwang BK  Kim BS 《Pest management science》2007,63(12):1208-1214

BACKGROUND: Microbial secondary metabolites are a rich source of antifungal agents and have merit as alternatives to synthetic fungicides. To develop disease control agents against powdery mildew, the lipopeptide antibiotic neopeptins were identified from the culture broth of a Streptomyces sp., and in vivo control efficacy of the compounds was evaluated on cucumber plants under glasshouse conditions. RESULTS: The Streptomyces sp. KNF2047 antagonistic against powdery mildew development in cucumber plants was isolated from a soil sample. Antifungal compounds were purified from the culture broth and identified as neopeptin A and B. In vitro microtitre assays revealed the inhibitory activities of the compounds in the range 128-512 microg mL(-1) against the mycelial growth of Alternaria mali, Botrytis cinerea, Cladosporium cucumerinum, Colletotrichum lagenarium, Didimella bryoniae and Magnaporthe grisea. Although neither compound showed remarkable in vitro antifungal activity against other plant pathogenic fungi, a mixture of neopeptins (484 mg of neopeptin A and 290 mg of neopeptin B per gram of partially purified powder) showed potent protective and curative activity against cucumber powdery mildew in vivo. The disease control activity of the neopeptins at a concentration of 2.4 mg L(-1) was 92.1%, which was similar to that of the commercial fungicide fenarimol (89.3% at 63 mg L(-1)) and that of the commercial biocontrol agent Actinovate (67.4% at 2 x 10(7) cfu L(-1)). CONCLUSION: Neopeptin mixtures isolated from Streptomyces sp. KNF2047 showed potent disease control activity against powdery mildew development on cucumber plants. .  相似文献   

Novel life stage targets against plum curculio, Conotrachelus nenuphar (Herbst), in apple integrated pest management     

Wise JC  Kim K  Hoffmann EJ  Vandervoort C  Gökçe A  Whalon ME 《Pest management science》2007,63(8):737-742

In this study the authors employed the plant-insect-chemistry (PIC) triad to investigate two novel life stage targets against the plum curculio (PC), Conotrachelus nenuphar (Herbst), in apple integrated pest management (IPM). Laboratory treated apple bioassays were used to determine if the insect growth regulator (IGR) insecticides novaluron and tebufenozide have physiological effects on PC larvae following adult exposure. Curative activity bioassays were conducted for IGR, neonicotinoid, oxidiazine and organophosphate insecticides on PC larvae post-infestation, and fruit penetration profiles of insecticides were developed. The results revealed that novaluron exhibits activity on PC larvae via vertical transmission following exposure of mated females to treated substrate. Surface treatments of azinphos-methyl, thiacloprid and thiamethoxam to preinfested fruit resulted in significant reductions in larval emergence. For all compounds, 50% or more of the total recovered active ingredient was from apple skin, and for azinphos-methyl, indoxacarb and thiamethoxam it was greater than 80%. For azinphos-methyl, novaluron, methoxyfenozide and thiacloprid, however, active ingredient was recovered from every section of the apple, from skin to core. Implications for twenty-first century IPM are discussed.  相似文献   

In vivo anti-influenza virus activity of an immunomodulatory acidic polysaccharide isolated from Cordyceps militaris grown on germinated soybeans   总被引：2，自引：0，他引：2  

Ohta Y  Lee JB  Hayashi K  Fujita A  Park DK  Hayashi T 《Journal of agricultural and food chemistry》2007,55(25):10194-10199

An acidic polysaccharide (APS) was isolated from the extract of Cordyceps militaris grown on germinated soybeans. Analyses of sugar composition indicated that APS consisted of d-galactose, L-arabinose, D-xylose, L-rhamnose, and D-galacturonic acid. On the basis of the result of methylation analysis, APS was considered to be mainly composed of Araf-(1-->, -->5)-Araf-(1-->, -->4)-Galp-(1--> and -->4)-GalAp-(1--> residues. When the polysaccharide was intranasally administered, it decreased virus titers in the bronchoalveolar lavage fluid and the lung of mice infected with influenza A virus and increased survival rate. Furthermore, APS increased TNF-alpha and IFN-gamma levels in mice when compared with those of untreated mice. APS enhanced nitric oxide (NO) production and induced iNOS mRNA and protein expressions in RAW 264.7 murine macrophage cells. The induction of mRNA expression of cytokines including IL-1beta, IL-6, IL-10, and TNF-alpha was also observed. These results demonstrated that APS might have beneficial therapeutic effects on influenza A virus infection at least in part by modulation of the immune function of macrophages.  相似文献   

What is your diagnosis? Ascites fluid from an 11‐year‐old dog with epigastric bulging     

Jae‐Ik Han  Hye‐Jin Jang  Dong‐Woo Chang  Gon‐Hyung Kim  Byeongwoo Ahn  Ki‐Jeong Na 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(4):541-544

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Infrared spectroscopy characterization of normal and lung cancer cells originated from epithelium     

So Yeong Lee  Kyong-Ah Yoon  Soo Hwa Jang  Erdene Ochir Ganbold  Dembereldorj Uuriintuya  Sang-Mo Shin  Pan Dong Ryu  Sang-Woo Joo 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(4):299-304

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