全文获取类型
收费全文 | 4863篇 |
免费 | 274篇 |
国内免费 | 2篇 |
专业分类
林业 | 199篇 |
农学 | 233篇 |
基础科学 | 13篇 |
816篇 | |
综合类 | 362篇 |
农作物 | 666篇 |
水产渔业 | 401篇 |
畜牧兽医 | 2139篇 |
园艺 | 69篇 |
植物保护 | 241篇 |
出版年
2023年 | 37篇 |
2022年 | 43篇 |
2021年 | 162篇 |
2020年 | 114篇 |
2019年 | 121篇 |
2018年 | 157篇 |
2017年 | 144篇 |
2016年 | 151篇 |
2015年 | 156篇 |
2014年 | 222篇 |
2013年 | 283篇 |
2012年 | 342篇 |
2011年 | 393篇 |
2010年 | 237篇 |
2009年 | 217篇 |
2008年 | 310篇 |
2007年 | 303篇 |
2006年 | 271篇 |
2005年 | 226篇 |
2004年 | 222篇 |
2003年 | 194篇 |
2002年 | 150篇 |
2001年 | 136篇 |
2000年 | 99篇 |
1999年 | 62篇 |
1998年 | 41篇 |
1997年 | 29篇 |
1996年 | 23篇 |
1995年 | 14篇 |
1994年 | 8篇 |
1993年 | 19篇 |
1992年 | 27篇 |
1991年 | 29篇 |
1990年 | 38篇 |
1989年 | 33篇 |
1988年 | 15篇 |
1987年 | 16篇 |
1986年 | 10篇 |
1985年 | 8篇 |
1984年 | 7篇 |
1983年 | 12篇 |
1982年 | 4篇 |
1980年 | 5篇 |
1978年 | 11篇 |
1974年 | 5篇 |
1972年 | 5篇 |
1971年 | 3篇 |
1970年 | 3篇 |
1969年 | 3篇 |
1968年 | 4篇 |
排序方式: 共有5139条查询结果,搜索用时 156 毫秒
991.
992.
993.
Dong-Kun Yang Byoung-han Kim Chang-Hee Kweon Jin-Ju Nah Hyun-Joo Kim Kyung-Woo Lee Young-Jin Yang Kyu-Whan Mun 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(4):381-385
Recent global warming trends may have a significant impact on vector-borne viral diseases, possibly affecting vector population dynamics and disease transmission. This study measured levels of hemagglutination-inhibition (HI) antibodies against Japanese encephalitis virus (JEV) and neutralizing antibodies against Akabane virus (AKAV) and Aino virus (AINV) for Thoroughbred horses in Korea. Blood samples were collected from 989 racehorses in several provinces, between October 2005 and March 2007. Sera were tested using either an HI assay or a virus neutralization test. Approximately half (49.7%; 492/989) of the horses tested were antibody-positive for JEV. The HI titer against JEV was significantly correlated with racehorse age (p < 0.05). Horses with an HI antibody titer of 1:160 or higher accounted for 3.9% of the animals tested, indicating that vectors transmitting arthropod-borne viruses bit relatively few horses. In contrast, 3.8% (19/497) and 19.5% (97/497) of horse sera collected in March 2007 were positive against AKAV and AINV, respectively. The presence of antibodies against AKAV and AINV may indicate the multiplication of AKAV and AINV in these horses. 相似文献
994.
Joon-Seok Chae Do-Hyeon Yu Smriti Shringi Terry A. Klein Heung-Chul Kim Sung-Tae Chong In-Yong Lee Janet Foley 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(3):285-293
A total of 1,618 ticks [420 individual (adults) and pooled (larvae and nymphs) samples], 369 rodents (Apodemus agrarius, Rattus norvegicus, Tscherskia triton, Mus musculus, and Myodes regulus), and 34 shrews (Crocidura lasiura) that were collected in northern Gyeonggi-do near the Demilitarized Zone (DMZ) of Korea during 2004-2005, were assayed by PCR for selected zoonotic pathogens. From a total of 420 individual and pooled tick DNA samples, Anaplasma (A.) phagocytophilum (16), A. platys (16), Ehrlichia (E.) chaffeensis (63), Borrelia burgdorferi (16), and Rickettsia spp. (198) were detected using species-specific PCR assays. Out of 403 spleens from rodents and shrews, A. phagocytophilum (20), A. platys (34), E. chaffeensis (127), and Bartonella spp. (24) were detected with species-specific PCR assays. These results suggest that fevers of unknown causes in humans and animals in Korea should be evaluated for infections by these vector-borne microbial pathogens. 相似文献
995.
Min-Won Baek Seung-Hyeok Seok Hui-Young Lee Dong Jae Kim Byoung-Hee Lee Young-Tae Ahn Kwang-Sei Lim Chul-Sung Huh Jae-Hak Park 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(1):31-37
An herbal extract mixture and yogurt added to the herbal extract mixture were tested for their protective and therapeutic effects on ethanol-induced liver injury. The herbal extract mixture, yogurt and commercial drugs were used for treatment for two weeks prior to administering a single oral dose of ethanol (3 g/kg body weight). The herbal extract mixture and yogurt added to the herbal extract mixture were found to provide protection against ethanol-induced toxicity comparable to the commercial drug treatment, according to the serum and histopathological analysis. It was also shown that co-treatment with herbal extract mixture and yogurt against a triple oral dose of ethanol (2 g/kg body weight, over one week) provided protection against ethanol toxicity. After the initial set of experiments, the herbal extract mixture and yogurt treatments were extended for three more weeks. When compared to the positive control, further treatment with both the herbal extract and yogurt significantly reduced liver injury and resulted in a lower grade of lipid deposition. 相似文献
996.
Dayoung Oh Siheon Lee Seungji Kim Sangkyung Choen Mincheol Choi Junghee Yoon 《Veterinary radiology & ultrasound》2019,60(1):28-37
Bronchomalacia and bronchial collapse are important causes of chronic coughing in dogs. The current reference standard diagnostic tests for these problems are flexible bronchoscopy and biopsy. Previous human studies have also supported inspiration/expiration computed tomography (CT) as a diagnostic test. The current prospective, pilot study aimed to determine whether inspiration/expiration CT is also a feasible test for quantifying bronchial collapsibility in dogs. Thoracic CT images were acquired using a 64‐row multidetector CT for 10 healthy Beagle dogs during maximal inspiration and expiration. For each scan, one observer measured transverse sectional areas of the mainstem and lobar bronchi, and the dorsal and ventral segmental bronchi of the left cranial lobar bronchus. Diameters for each bronchus were also measured in transverse, sagittal, and dorsal planes. Bronchial collapsibility (%) was calculated as the difference between inspiration/expiration transverse sectional areas divided by the inspiration transverse sectional areas. Mean bronchial collapsibility of all bronchi was 38.20 ± 15.17%. A collapsibility of over 50% was found in the dorsal (n = 7) and ventral (n = 4) segmental bronchi of the left cranial lobar bronchus, and the left caudal (n = 5) and right middle (n = 2) lobar bronchus. Bronchial collapsibility measurements were greater in the dorsal and ventral segmental bronchi of the left cranial lobar bronchus and the left caudal lobar bronchus (P < 0.001). Findings supported inspiration/expiration CT as a modality to noninvasively assess bronchial collapse in dogs and a bronchial collapsibility value greater than 50% for detecting pathologic bronchial collapse in clinically affected dogs. 相似文献
997.
Prabhu Balan Yuan H. Brad Kim Adam D. Stuart Robert Kemp Maryaan Staincliffe Cameron Craigie Mustafa M. Farouk 《Animal Science Journal》2019,90(8):1060-1069
The objective of this study was to determine the effect of two different freezing rate then thaw‐aging regimens on the quality attributes of lamb loins. The loins were randomly allocated to one of five different freezing/thawing/aging regimes: fast‐(FF1A0) and slow‐(SF1A0) frozen only; fast‐(FF1A2) and slow‐(SF1A2) frozen then thaw‐aged for 14 days; aged for 14 days never frozen (A2). FF1A2 samples had a significantly higher water‐holding capacity compared to the slow frozen regardless of further aging periods. FF1A2 samples had lower (p < 0.05) shear force values than A2 and higher (p < 0.05) water‐holding capacity compared to the SF1A2. Fast freezing resulted in more intracellular cryo‐damage, whereas slow freezing resulted in extracellular cryo‐damage. FF1A0 and SF1A0 samples had lower (p < 0.05) myofibrillar proteins degradation. This study demonstrated that fast freezing then thaw‐aging can result in an improved water‐holding capacity and tenderness through the minimization of extracellular ice crystal formation, reduction in purge and drip losses, and improved proteolysis in thawed lamb. 相似文献
998.
Prabhu Balan Mustafa M. Farouk Adam D. Stuart Robert Kemp Maryaan Staincliffe Cameron Craige Yuan H. B. Kim 《Animal Science Journal》2019,90(8):1050-1059
The objective of this study was to create various pH/temp decline rates in hot‐boned bull beef M. longissimus lumborum (LL) through a combination of electrical stimulation (ES) and pre‐rigor holding temperature. The relationship between the pre‐rigor interventions, the activities of µ‐calpain and small heat shock proteins (sHSP), and the impacts on meat product quality were determined. Paired LL loins from 13 bulls were hot‐boned within 40 min of slaughter, immediately ES and subjected to various holding temperatures (5, 15, 25, and 35°C) for 3 hr. The rate of muscle pH decline, sarcomere length, shear force, and proteolysis of muscle proteins were measured. ES‐25°C had a longer sarcomere length compared to non‐electrical stimulation samples. ES‐25°C and ES‐35°C samples had lower shear force values, higher µ‐calpain activity and higher desmin, troponin‐T, and sHSP degradation. The above findings suggest that pH/temp decline rates created in hot‐boned muscle impacted muscle protein proteolysis by increasing the activity of proteases and degradation of sHSP. 相似文献
999.
Thanh‐Van Nguyen Lanh Thi Kim Do Tamas Somfai Takeshige Otoi Masayasu Taniguchi Kazuhiro Kikuchi 《Animal Science Journal》2019,90(12):1530-1536
Chlorogenic acid (CGA) is known to protect oocytes from oxidative stress. Here we investigated the effects of CGA on porcine oocyte maturation under heat stress and subsequent embryonic development after parthenogenetic activation. For in vitro maturation (IVM) at 41.0°C (hyperthermic condition), supplementation of the maturation medium with 50 μM CGA significantly improved the percentage of matured oocytes and reduced the rate of apoptosis relative to oocytes matured without CGA (p < .05). CGA treatment of oocytes during IVM under hyperthermia tended to increase (p < .1) percentage of blastocyst formation after parthenogenesis and significantly increased (p < .05) the total cell number per blastocyst relative to oocytes matured without CGA. For IVM at 38.5°C (isothermic condition), CGA significantly improved the rate of blastocyst development compared with oocytes matured without CGA (p < .05), but did not affect oocyte maturation, apoptosis rate or the number of cells per embryo. Omission of all antioxidants from the IVM medium significantly reduced the rate of oocyte maturation, but the rate was restored upon addition of CGA. These results demonstrate that CGA is a potent antioxidant that protects porcine oocytes from the negative effects of heat stress, thus reducing the frequency of apoptosis and improving the quality of embryos. 相似文献
1000.
Jeong Hyo Lee Jeong‐Woong Park Eun Sol Seo Hoy‐Ung Kim Seo Woo Kim Ji Seon Han Hyun Sik Jun Sung‐Jo Kim Tae Sub Park Byung‐Chul Park 《Animal Science Journal》2019,90(7):903-912
The value‐added products in livestock industry is one of the key issues in order to maximize the revenue and to create a new business model. Numerous studies have suggested application of herbal plants as feed additives to increase health, productivity, and/or high‐quality product in livestock. In this study, the first experiment was designed to develop in vitro evaluation system by using primary chicken myoblast (pCM) cells isolated from pectoralis major of 10‐day‐old male embryos. Subsequently, to evaluate effects of Korean Danggui Angelica gigas Nakai (AGN), we optimized the concentration of AGN root extract for treatment of primary pCM cells. After the treatment of AGN root extract, we compared proliferation and differentiation capacity, and also examined the gene expression. In the second experiment, the next generation sequencing analysis was performed to compare the different patterns of the global gene expression in pCM cells treated with AGN extract. Three up‐regulated (pancreas beta cells, fatty acid metabolism and glycolysis) and one down‐regulated (adipogenesis) gene sets were characterized suggesting that the AGN extract affected the metabolic pathways for the utilization of fat and glucose in chicken muscle cells. Furthermore, we validated the expression patterns of the up‐regulated genes (GCLC, PTPN6, ISL1, SLC25A13, TGFBI, and YWHAH) in the AGN‐treated pCM cells by quantitative RT‐PCR. These results demonstrated that the treatment of AGN extract decreased proliferation and differentiation of pCM cells, and affected the metabolic pathways of glucose and fatty acids. Moreover, AGN extract derived from byproducts such as stem and leaf also showed the reduced proliferation patterns on AGN‐treated pCM cells. Taken together, pCM cell‐based in vitro assay system could be primarily and efficiently applied for evaluating the biofunctional efficacy of various feed additive candidates. 相似文献