Effect of fast freezing then thaw‐aging on meat quality attributes of lamb M. longissimus lumborum     

Prabhu Balan  Yuan H. Brad Kim  Adam D. Stuart  Robert Kemp  Maryaan Staincliffe  Cameron Craigie  Mustafa M. Farouk 《Animal Science Journal》2019,90(8):1060-1069

The objective of this study was to determine the effect of two different freezing rate then thaw‐aging regimens on the quality attributes of lamb loins. The loins were randomly allocated to one of five different freezing/thawing/aging regimes: fast‐(FF1A0) and slow‐(SF1A0) frozen only; fast‐(FF1A2) and slow‐(SF1A2) frozen then thaw‐aged for 14 days; aged for 14 days never frozen (A2). FF1A2 samples had a significantly higher water‐holding capacity compared to the slow frozen regardless of further aging periods. FF1A2 samples had lower (p < 0.05) shear force values than A2 and higher (p < 0.05) water‐holding capacity compared to the SF1A2. Fast freezing resulted in more intracellular cryo‐damage, whereas slow freezing resulted in extracellular cryo‐damage. FF1A0 and SF1A0 samples had lower (p < 0.05) myofibrillar proteins degradation. This study demonstrated that fast freezing then thaw‐aging can result in an improved water‐holding capacity and tenderness through the minimization of extracellular ice crystal formation, reduction in purge and drip losses, and improved proteolysis in thawed lamb.  相似文献   

Effects of electrical stimulation and pre‐rigor conditioning temperature on aging potential of hot‐boned beef M. longissimus lumborum     

Prabhu Balan  Mustafa M. Farouk  Adam D. Stuart  Robert Kemp  Maryaan Staincliffe  Cameron Craige  Yuan H. B. Kim 《Animal Science Journal》2019,90(8):1050-1059

The objective of this study was to create various pH/temp decline rates in hot‐boned bull beef M. longissimus lumborum (LL) through a combination of electrical stimulation (ES) and pre‐rigor holding temperature. The relationship between the pre‐rigor interventions, the activities of µ‐calpain and small heat shock proteins (sHSP), and the impacts on meat product quality were determined. Paired LL loins from 13 bulls were hot‐boned within 40 min of slaughter, immediately ES and subjected to various holding temperatures (5, 15, 25, and 35°C) for 3 hr. The rate of muscle pH decline, sarcomere length, shear force, and proteolysis of muscle proteins were measured. ES‐25°C had a longer sarcomere length compared to non‐electrical stimulation samples. ES‐25°C and ES‐35°C samples had lower shear force values, higher µ‐calpain activity and higher desmin, troponin‐T, and sHSP degradation. The above findings suggest that pH/temp decline rates created in hot‐boned muscle impacted muscle protein proteolysis by increasing the activity of proteases and degradation of sHSP.  相似文献   

Presence of chlorogenic acid during in vitro maturation protects porcine oocytes from the negative effects of heat stress     

Thanh‐Van Nguyen  Lanh Thi Kim Do  Tamas Somfai  Takeshige Otoi  Masayasu Taniguchi  Kazuhiro Kikuchi 《Animal Science Journal》2019,90(12):1530-1536

Chlorogenic acid (CGA) is known to protect oocytes from oxidative stress. Here we investigated the effects of CGA on porcine oocyte maturation under heat stress and subsequent embryonic development after parthenogenetic activation. For in vitro maturation (IVM) at 41.0°C (hyperthermic condition), supplementation of the maturation medium with 50 μM CGA significantly improved the percentage of matured oocytes and reduced the rate of apoptosis relative to oocytes matured without CGA (p < .05). CGA treatment of oocytes during IVM under hyperthermia tended to increase (p < .1) percentage of blastocyst formation after parthenogenesis and significantly increased (p < .05) the total cell number per blastocyst relative to oocytes matured without CGA. For IVM at 38.5°C (isothermic condition), CGA significantly improved the rate of blastocyst development compared with oocytes matured without CGA (p < .05), but did not affect oocyte maturation, apoptosis rate or the number of cells per embryo. Omission of all antioxidants from the IVM medium significantly reduced the rate of oocyte maturation, but the rate was restored upon addition of CGA. These results demonstrate that CGA is a potent antioxidant that protects porcine oocytes from the negative effects of heat stress, thus reducing the frequency of apoptosis and improving the quality of embryos.  相似文献   

Functional efficacy analysis of Angelica gigas Nakai on chicken myoblast cells through cell‐based in vitro assay     

Jeong Hyo Lee  Jeong‐Woong Park  Eun Sol Seo  Hoy‐Ung Kim  Seo Woo Kim  Ji Seon Han  Hyun Sik Jun  Sung‐Jo Kim  Tae Sub Park  Byung‐Chul Park 《Animal Science Journal》2019,90(7):903-912

The value‐added products in livestock industry is one of the key issues in order to maximize the revenue and to create a new business model. Numerous studies have suggested application of herbal plants as feed additives to increase health, productivity, and/or high‐quality product in livestock. In this study, the first experiment was designed to develop in vitro evaluation system by using primary chicken myoblast (pCM) cells isolated from pectoralis major of 10‐day‐old male embryos. Subsequently, to evaluate effects of Korean Danggui Angelica gigas Nakai (AGN), we optimized the concentration of AGN root extract for treatment of primary pCM cells. After the treatment of AGN root extract, we compared proliferation and differentiation capacity, and also examined the gene expression. In the second experiment, the next generation sequencing analysis was performed to compare the different patterns of the global gene expression in pCM cells treated with AGN extract. Three up‐regulated (pancreas beta cells, fatty acid metabolism and glycolysis) and one down‐regulated (adipogenesis) gene sets were characterized suggesting that the AGN extract affected the metabolic pathways for the utilization of fat and glucose in chicken muscle cells. Furthermore, we validated the expression patterns of the up‐regulated genes (GCLC, PTPN6, ISL1, SLC25A13, TGFBI, and YWHAH) in the AGN‐treated pCM cells by quantitative RT‐PCR. These results demonstrated that the treatment of AGN extract decreased proliferation and differentiation of pCM cells, and affected the metabolic pathways of glucose and fatty acids. Moreover, AGN extract derived from byproducts such as stem and leaf also showed the reduced proliferation patterns on AGN‐treated pCM cells. Taken together, pCM cell‐based in vitro assay system could be primarily and efficiently applied for evaluating the biofunctional efficacy of various feed additive candidates.  相似文献   

Extracellular cyclic adenosine monophosphate‐dependent protein kinase A autoantibody and C‐reactive protein as serum biomarkers for diagnosis of cancer in dogs     

Min‐Ok Ryu  Byung‐Gak Kim  Ul‐Soo Choi  Kwan‐Hyuck Baek  Young‐Ki Song  Qiang Li  Kyoung‐Won Seo  Sandra Ryeom  Hwa‐Young Youn  Dong‐Ha Bhang 《Veterinary and comparative oncology》2019,17(1):99-106

Protein kinase A, a cyclic adenosine monophosphate (AMP)‐dependent enzyme, normally exists within mammalian cells; however, in cancer cells, it can leak out and be found in the serum. Extracellular cyclic AMP‐dependent protein kinase A (ECPKA) has been determined to increase in the serum of cancer‐bearing dogs. However, there have been no reports in the veterinary literature on serum ECPKA autoantibody (ECPKA‐Ab) expression in dogs with cancer. The aim of this study was to evaluate ECPKA‐Ab and C‐reactive protein (CRP) as serum biomarkers for cancer in dogs. ECPKA‐Ab and CRP levels were detected by an enzyme‐linked immunosorbent assay in serum samples from dogs with malignant tumours (n = 167), benign tumours (n = 42), or non‐tumour disease (n = 155) and from healthy control dogs (n = 123). ECPKA‐Ab and CRP levels were significantly higher in the dogs with malignant tumours than in those with benign tumours or non‐tumour diseases, as well as in the healthy controls (P < 0.001, Kruskal‐Wallis test). There was a significant positive correlation between the neoplastic index, which was developed using ECPKA‐Ab and CRP levels, and the presence of cancer in dogs (P < 0.001); the area under the receiver‐operating characteristic curve was estimated to be >0.85 (P < 0.001). In conclusion, ECPKA‐Ab is a potential serum biomarker for a broad spectrum of cancers. Combined measurement of CRP and ECPKA‐Ab levels in serum improves the sensitivity and accuracy of a diagnosis of cancer in dogs.  相似文献   

Effect of intra‐abdominal hypertension on plasma exogenous creatinine clearance in conscious and anesthetized dogs     

Min Jang  Won‐Gyun Son  Sang‐Min Jo  Hyunseok Kim  Chi Won Shin  Inhyung Lee 《Journal of Veterinary Emergency and Critical Care》2019,29(4):366-372

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US Sheep Industry and the Public Grazing Fee     

Ryan Feuz  Man-Keun Kim 《Strength and Conditioning Journal》2019,72(3):415-424

Since the mid-1940s US sheep inventory has experienced dramatic declines, which has weakened the sheep industry significantly, making it increasingly important to analyze potential policy implications that could affect US sheep inventories in the future. Public grazing lands are often used in sheep production, especially within the western United States. The public grazing fee is, therefore, a cost within the production of sheep. A US sheep model applying capital stock inventory accounting methodology is developed to model both the supply and demand within the joint sheep and wool industries. The model is used to create a baseline projection for the next several years. Various public grazing fee policies are created to demonstrate the effects of the policies on the levels of sheep inventory and sheep and wool production within the country. Results indicate removing the public grazing fee (set at $0/animal unit month) may slow the rate of decline but would not be effective at reversing the declining trend. This suggests reducing the public grazing fee is not a viable policy option to help bring stability to the sheep and wool industries. However, projections indicate raising the grazing fee would have a substantial adverse effect on the industries. Consideration should be given to these results as grazing fee policy is adjusted moving forward.  相似文献   

Fumigant toxicity of volatile natural products from Korean spices and medicinal plants towards the rice weevil,Sitophilus oryzae (L)     

Sung‐Eun Lee  Byoung‐Ho Lee  Won‐Sik Choi  Byeoung‐Soo Park  Jeong‐Gyu Kim  Bruce C Campbell 《Pest management science》2001,57(6):548-553

The fumigant toxicity of various volatile constituents of essential oils extracted from sixteen Korean spices and medicinal plants towards the rice weevil, Sitophilus oryzae L (Coleoptera: Curculionidae), was determined. The most potent toxicity was found in the essential oil from Mentha arvensis L var piperascens (LC₅₀ = 45.5 µl litre^?1 air). GC–MS analysis of essential oil from M arvensis showed it to be rich in menthol (63.2%), menthone (13.1%) and limonene (1.5%), followed in abundance by β‐pinene (0.7%), α‐pinene (0.6%) and linalool (0.2%). Treatment of S oryzae with each of these terpenes showed menthone to be most active (LC₅₀ = 12.7 µl litre^?1 air) followed by linalool (LC₅₀ = 39.2 µl litre^?1 air) and α‐pinene (LC₅₀ = 54.9 µl litre^?1 air). Studies on inhibition of acetylcholinesterase activity of S oryzae showed menthone to have a nine‐fold lower inhibitory effect than menthol, despite menthone being 8.1‐fold more toxic than menthol to the rice weevil. Different modes of toxicity of these monoterpenes towards S oryzae are discussed. © 2001 Society of Chemical Industry  相似文献   

Response     

Kim NW  Harley CB  Prowse KR  Weinrich SL  Piatyszek MA  Wright WE  Shay JW 《Science (New York, N.Y.)》1995,268(5214):1194-1117

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The effects of canthaxanthin on porcine oocyte maturation and embryo development in vitro after parthenogenetic activation and somatic cell nuclear transfer          下载免费PDF全文

A Taweechaipaisankul  JX Jin  S Lee  GA Kim  BC Lee 《Reproduction in domestic animals》2016,51(6):870-876

The objective of this study was to examine the effects of canthaxanthin (Cx) treatment during in vitro maturation (IVM) of porcine oocytes on embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT), on intracellular glutathione (GSH) and reactive oxygen species (ROS) levels in mature oocytes, and on gene expression in both PA‐ and SCNT‐derived blastocysts. To determine the optimal effective concentration of Cx, porcine oocytes were cultured in IVM medium supplemented with various concentrations (0, 20, 40 and 80 μM) of Cx for 22 hr. Compared to other groups, supplementation with 40 μM Cx significantly improved blastocyst formation rates after PA (p < .05), but no significant differences were observed among groups in total blastocyst cell numbers. Subsequently, oocytes were cultured in IVM medium supplemented with or without 40 μM Cx. Oocytes treated with 40 μM Cx showed significantly increased cleavage and blastocyst formation rates after SCNT compared to the control group (p < .05). Moreover, significantly increased intracellular GSH and reduced ROS levels were observed in the Cx‐treated group (p < .05). In addition, both PA‐ and SCNT‐derived blastocysts from the 40 μM Cx‐treated group showed significantly increased mRNA expression of Bcl2 and Oct4 and decreased Caspase3 expression level (p < .05), when compared with the control group. PA‐derived blastocysts from the 40 μM Cx‐treated group also exhibited significantly decreased expression of Bax (p < .05). Our results demonstrated that treatment with 40 μM Cx during IVM improves the developmental competence of PA and SCNT embryos. Improvement of embryo development by Cx is most likely due to increased intracellular GSH synthesis, which reduces ROS levels in oocytes, and it may also positively regulate apoptosis‐ and development‐related genes.  相似文献